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本试验从新疆石河子地区某规模化猪场采集疑似由副猪嗜血杆菌引起的病变组织,进行实验室分离鉴定,确诊为副猪嗜血杆菌致病。对患病的3头猪进行治疗发现,恩诺沙星、氟苯尼考、林克霉素对该病都有很好的效果,对于感染严重的猪只建议使用头孢喹肟、头孢噻呋,同时建议对全群用恩诺沙星和替米考星配合拌料或用氟苯尼考和替米考星配合拌料进行药物预防。规模化猪场为防止该病的发生,还应选择正规厂家的药物进行治疗,注意猪场的严格消毒,做好猪繁殖与呼吸综合征和猪圆环病毒2型的免疫,防止继发感染。 相似文献
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为了解猪胸膜肺炎放线杆菌对达氟沙星的敏感性和达氟沙星的杀菌效果,收集和分离48株猪胸膜肺炎放线杆菌,采用微量肉汤稀释法和菌落计数法对体外药物敏感性试验和生长曲线、杀菌曲线进行了研究。猪胸膜肺炎放线杆菌对氨苄西林、磺胺异恶唑、头孢噻呋、大观霉素、黏菌素耐药率高,对阿莫西林/克拉维酸、复方新诺明、氟苯尼考、多西环素、庆大霉素、四环素、恩诺沙星、氧氟沙星、达氟沙星敏感。达氟沙星最小抑菌浓度(MIC)集中在0.0078μg/m L。达氟沙星浓度在MIC及以上时,随浓度升高,杀菌时间缩短,为典型的浓度依赖型抗生素。该试验结果可用于指导猪传染性胸膜肺炎的临床预防和治疗。 相似文献
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《养殖与饲料.饲料世界》2018,(1)
正1)治疗细菌全身感染的药物。头孢噻呋、阿莫西林、阿米卡星、沙拉沙星等。2)治疗败血型大肠杆菌的药物。头孢类、氟苯尼考、阿米考星、沙拉沙星、二氟沙星等。3)治疗肠炎型大肠杆菌的药物。安普霉素、新霉素、粘杆菌素、烟酸诺氟沙星、庆大霉素等。4)治疗支原体的药物。罗红霉素、泰乐菌素、泰妙菌素、替米考星、红霉素、氧氟沙星、强力霉素等。 相似文献
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为了观察地锦草与阿莫西林等抗菌药物联用对鸡致病性大肠杆菌的体外抑菌效果,试验采用琼脂稀释法分别测定地锦草与10种抗菌药物联用的抑菌作用,并与地锦草和抗菌药物单独应用时的抑菌效果相比较。结果表明:12株大肠杆菌对抗菌药物呈现多重耐药性,地锦草对大肠杆菌的最小抑菌浓度范围为7.812 5~31.250 mg/mL;地锦草与头孢曲松、头孢噻呋、氟苯尼考、多西环素联用时的抑菌协同率为58.3%~83.3%,与阿米卡星、大观霉素、恩诺沙星、黏菌素联用时的抑菌颉颃率为58.3%~100%,与阿莫西林、安普霉素联用时显示无关或颉颃作用。说明地锦草可以增强头孢曲松、头孢噻呋、氟苯尼考、多西环素对大肠杆菌的抑菌作用,可以颉颃阿米卡星、大观霉素、恩诺沙星、黏菌素对大肠杆菌的抑菌作用。 相似文献
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《畜牧与兽医》2016,(4):116-118
从闽北地区16个规模化猪场分离到5株病原菌,经过分离纯化、形态学观察、细菌生化鉴定、PCR鉴定以及测序分析,分离菌株与已知的副猪嗜血杆菌(Haemophilus parasuis,Hps)菌株16S rRNA序列同源性均在96%以上,确定5株病原菌均为副猪嗜血杆菌。通过致病性试验表明:分离菌株均有一定的致病性,其中Hps-jo株分离菌致死率达83.3%。细菌药敏试验表明:分离菌株对头孢噻呋、头孢氨苄、氟苯尼考敏感;对万古霉素、强力霉素、四环素、多黏菌素中度敏感;对环丙沙星、阿莫西林、恩诺沙星低、链霉素、TMP、新霉素耐药率达100%。试验结果为闽西地区副猪嗜血杆菌病的防治提供了参考。 相似文献
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广东地区鸭疫里氏杆菌的血清型及抗药性情况调查 总被引:4,自引:0,他引:4
对2006~2008年间从广东省规模化鸭场罹患鸭传染性浆膜炎的病鸭中分离的鸭疫里氏杆菌的血清型和抗药性流行情况进行了调查和分析。在122个鸭场中共分离鉴定出鸭疫里氏杆菌86株。平板凝集试验结果表明血清1型菌株有81株,占总分离株的94.18%;其他血清型有8型2株,4型、3型和10型各1株,提示血清1型仍是广东省流行的优势血清型。以纸片法测定所分离菌株对氟苯尼考等13种常用抗菌药物的敏感性,结果显示广东地区鸭疫里氏杆菌抗药性严重,其中仅对阿莫西林、氨苄青霉素、头孢噻吩和氟苯尼考相对敏感,敏感菌株的比例分别为82.55%、77.91%、82.55%和81.39%,对其他抗菌药物均有抗性,75.58%的菌株同时对5种以上的抗菌药物具有多重抗药性,且不同地区鸭疫里氏杆菌分离株的药物敏感性也存在明显差异。 相似文献
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对安徽省某鸭场病鸭的临床症状及剖检病变进行分析和细菌分离,并对分离的细菌进行形态培养、PCR检测和克隆测序,初步鉴定疑似为鸭疫里氏杆菌的有2株,分别命名为AH-1和AH-2,同时将测序结果与GenBank上公布的序列AY606207进行比对,结果发现AH-1和AH-2与其同源性分别为97.0%和100.0%。给健康鸭注射该分离茵可复制出心包炎、肝周炎、气囊炎等典型病理变化并引起死亡。药敏试验结果表明,这2株分离菌对头孢唑林、阿莫西林、氟苯尼考、氨苄青霉素高度敏感,对罗红霉素、利福平中度敏感,对庆大霉素、丁胺卡那、红霉素、氧氟沙星、强力霉素不敏感,对链霉素、恩诺沙星、环丙沙星完全耐药。 相似文献
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甘肃省CSF、PCV-2、PRRS、PR和PCP的流行病学调查与分析 总被引:1,自引:0,他引:1
对甘肃省内规模和散养临床发病猪病料,采用PCR和细菌分离鉴定方法进行CSFV、PR、PRRS和PCV-2四种主要病毒病病原学检测和PCP放线杆菌分离鉴定。5年共检测病死猪病料1213份,CSF、PCV-2、PRRS、PR、PCP的平均阳性率分别为42.8%、34.3%、31.8%、30.2%、33.5%;5年共检测养殖场/户326个,5种病的检出率分别为62.6%、15.3%、14.7%、18.4%、21.5%。养殖场/户猪群疫病的感染以混合感染为主,混合感染占检测病例总数的62.6%,五种病混合感染分别占各自感染例数的为54.9%、92.0%、75.0%、80.0%、82.9%。其中二重感染占80.4%。形式以CSF+PCP、CSF+PR、CSF+PRRS和PCV-2+PRRS居多,CSF单独感染占所有单独感染病例的75.4%。 相似文献
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Bosi P Merialdi G Scandurra S Messori S Bardasi L Nisi I Russo D Casini L Trevisi P 《Journal of animal science》2011,89(12):4043-4053
The aim of this study was to determine the effects of 3 antibiotics used for pulmonary pathologies added in the feed of weaned pigs on growth performance, commensal microbiota, and immune response. At weaning, a total of 72 pigs were randomly assigned by BW and litter to 1 of the following diets: control (typical weaning diet), control + 400 mg of tilmicosin/kg, control + 600 mg of amoxicillin/kg, and control + 300 mg of doxycycline/kg. Individually penned pigs were slaughtered after 3 wk (12 pigs/treatment) or 4 wk (6 pigs/treatment). During the fourth week, all pigs received the control diet to test the residual effect of the antimicrobial supplementation. The antibiotic supplementation increased growth and feed intake during the first week (P < 0.01) and over the first 3 wk combined (P < 0.05). Gain-to-feed ratio tended to improve during the first week (P = 0.076) by the antibiotics compared with the control. Among the antibiotic treatments, no difference was observed in ADG and feed intake, which were also unchanged by the diet in the fourth week. The fecal enterobacteria counts were increased by amoxicillin on d 14 and 21 (P < 0.05 and 0.01, respectively) and were decreased by tilmicosin (P < 0.001) compared with the control. Amoxicillin decreased lactic acid bacteria (P < 0.01) counts compared with the control. The antibiotic supplementation tended to decrease total bacteria variability in the jejunum (Shannon index, P = 0.091) compared with the control. The antibiotic treatment decreased the mean total serum IgM concentration (P = 0.016) after 3 wk and did not change the mucosal histomorphometry of the small intestine. For tilmicosin, the observed positive action on piglet performance and feed intake can originate by the decreased costs of immune activation determined by the action on intestinal microbiota. For amoxicillin and doxycycline, the observation on intestinal and fecal microbiota seems to be not sufficient to explain their growth-promoting effect. 相似文献
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Yoshimura H Ishimaru M Endoh YS Kojima A 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(7):555-560
Minimum inhibitory concentrations (MICs) of 10 antimicrobial agents were determined for Pasteurella multocida from cattle and pigs (72 and 68 isolates, respectively). Higher MICs were observed with oxytetracycline, doxycycline, tilmicosin and thiamphenicol for porcine isolates than for bovine isolates. Enrofloxacin was the most active, with an MIC for 90% of the isolates (MIC90) of 0.05 microg/ml for both bovine and porcine isolates. Aspoxicillin exhibited the same excellent activity against penicillin-susceptible isolates as ceftiofur, with MICs ranging from < or = 0.025 to 0.1 microg/ml. Aminoglycosides were less active, with an MIC90 of > 100 microg/ml for both bovine and porcine isolates. 相似文献
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对甘肃省健康规模猪场和发病(非流感)规模猪场进行H1N1和H3N2猪流感(SI)感染抗体检测,健康规模猪场H1N1SI和H3N2SI混合感染抗体阳性率为25.45%(28/110);发病(非流感)规模猪场H1N1SI和H3N2SI混合感染抗体阳性率为25.97%(20/77)。对两个规模猪场1、6、7和10月份的抗体检测结果,平均抗体阳性率分别为21.27%、56.14%、75%和15.62%。H1N1和/或H3N2SI阳性猪场猪传染性胸膜肺炎(APP)、猪繁殖与呼吸综合征(PARS)、猪圆环病毒病(PCV-2)和猪支原体肺炎(Mhyo)感染抗体平均阳性率依次分别为50.79%、40.32%、43.82%和33.33%;猪传染性胸膜肺炎(APP)免疫抗体平均保护率为49.5%,猪繁殖与呼吸综合征(PRRS)、猪圆环病毒病(PCV-2)和猪支原体肺炎(Mhyo)免疫抗体阳性率依次分别为56.52%、47.92%和38.04%。 相似文献
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为确诊贵州省都匀市某养殖场的猪死亡病因,采集病死猪病料进行猪繁殖与呼吸综合征病毒、猪瘟病毒、猪伪狂犬病病毒、猪圆环病毒2型核酸检测,细菌分离鉴定及药物敏感试验。结果:猪繁殖与呼吸综合征病毒实时荧光定量RT-PCR检测和猪圆环病毒2型PCR检测核酸均为阳性,其余病毒核酸检测均为阴性。细菌分离培养表明存在细菌感染,分子生物学鉴定为奇异变形杆菌;分离菌对硫酸安普霉素、硫酸粘菌素、盐酸大观霉素+盐酸林可霉素、氟苯尼考较敏感,对多种药物产生耐药。结论:确诊病例为猪繁殖与呼吸综合征病毒、猪圆环病毒2型、奇异变形杆菌混合感染。 相似文献
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H J Cho B McNab C Dubuc L Jordan A Afshar R Magar S Prins K Eernisse 《Canadian journal of veterinary research》1997,61(3):161-166
A comparison was made of serological diagnostic methods used for the detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus. In the "phase I" PRRS test panel comparison, a panel of sera collected from 135 pigs of various ages, from North American herds with and without PRRS histories, were sent to 4 different laboratories and tested by an indirect immunofluorescent assay (IFA), an immunoperoxidase monolayer assay (IPMA) and an indirect enzyme-linked immunosorbent assay (iELISA). In the "phase II" PRRS test panel comparison, a panel of 382 sera collected from pigs of various ages, PRRS histories, and from various locations in North America and France, were divided into 2 panels (A & B) and sent to 3 Canadian laboratories and tested by the IFA and iELISA. In the phase I comparison, agreement between the IFA of laboratory 4 and the iELISA and IPMA of laboratory 3 was excellent (kappa values of 95% and 98%, respectively). This contrasted with the poor agreement between these laboratories and the IFA results of laboratories 1 and 2 in the phase I trial. In the phase II comparison, the results demonstrated good agreement between various tests both within and between laboratories. The overall performance of the iELISA was superior in the combination of sensitivity (96.1%) and specificity (100%) relative to the reference classification of the serum samples and repeatability (kappa value 98%). The iELISA is technically superior to IFA and IPMA, time efficient, cost effective and suitable for testing of a large number of samples over a short period of time. Thus, the iELISA may be a better alternative to IFA or IPMA for routine detection of PRRS viral antibodies in swine sera. 相似文献