共查询到19条相似文献,搜索用时 265 毫秒
1.
紫苏叶注射液的制备(英文) 总被引:1,自引:0,他引:1
[Objective] To extract the effective component rosmarinic acid from Folium perillae,study preparation technology and quality control of rosmarinic acid injection and provide test basis for hemorheological drug development and clinic application.[Method]The coarse powder of Folium perillae(40 μm)was lixiviated with hot water and acidized to make aqueous extracts.Rosmarinic acid from the aqueous mixture was extracted with Ethyl Acetate.And Et Ac was evaporated to obtain primary product.The injection was purified by pH adjustment.Concentration of rosmarinic acid in the injection was detected by high performance liquid chromatography(HPLC).Contents of tannin,resin and oxalate were eliminated according to the Chinese Pharmacopeia(2005 edition).Stability,irritation,haemolyticus,LD50 and thermogenic substance of this injection were also detected.[Result]The content of rosmarinic acid in Folium perillae injection is 2.81 mg/ml,and the content of LD50 was 406.82 mg/kg.This injection was consistent with the stipulation of Chinese Pharmacopeia(2005 edition).[Conclusion]The preparation technology of this experiment was reasonable.The stable qualities of the prepared injection meet the injection requirement. 相似文献
2.
基于均匀设计优化牛皮杜鹃嫩叶直接再生芽苗及植株再生体系(英文) 总被引:1,自引:0,他引:1
[Objective] The experimental was aimed to screen the optimum regeneration shoot induction media and rooting media for tender leaves of Rhododendron chrysanthum Pall.[Method] The tender leaves of Rhododendron chrysanthum Pall were taken as explants to select the optimum bud induction media and rooting media through uniform design and the screening results were verified.[Result] The optimum media for regeneration shoot of Rhododendron chrysanthum Pall contained 1/4 MS,3.70 mg/L ZT, 0.02 mg/L IAA and 1.00 mg/L KT and its induction rate was 95.5% and the rooting media contained modified MS, 0.10 mg/L IAA and 0.07 mg/L NAA and its rooting rate was 98%. [Conclusion] Through this experiment, regeneration systems for regeneration shoot and regenerated plant from tender leaves of Rhododendron chrysanthum Pall were created successfully. 相似文献
3.
铜绿假单胞菌脂肪酶Lipase基因的原核表达(英文) 总被引:4,自引:1,他引:3
[Objective] The aim of this study was to investigate the prokaryotic expression of pseudomonas aeruginosa Lipase gene.[Method]Lipase gene was amplified by PCR from the genome DNA of pseudomonas aeruginosa,and its nucleotide sequence was determined.The prokaryotic expression vector of Lipase gene was constructed by the gene recombination technique.The protein expression was induced for 4 hours by IPTG with the final concentration of 1.0 mmol/L,and then SDS-PAGE electrophoresis was analyzed.[Result]The sequence of mature peptides in Lipase gene cloned from pseudomonas aeruginosa had a 99.36% homology with that of pseudomonas aeruginosa lipase submitted in NCBI,so the prokaryotic expression vector of Lipase gene pET32a-Lip was successfully constructed.Furthermore,the results of SDS-PAGE electrophoresis showed that the target gene was expressed highly and effectively.[Conclusion]The cloned pseudomonas aeruginosa lipase with its signal peptide could be normally expressed in E.coli and also used for further study. 相似文献
4.
牛瑟氏泰勒虫P23基因的克隆与生物信息学分析(英文) 总被引:3,自引:0,他引:3
[Objective] The aim of this study is to provide basis for developing genetic engineering vaccine and diagnostic kit for Theileria sergenti infection. [Objective] P23 gene of Theileria sergenti was amplified from its genomic DNA by PCR amplification, and cloned into the pGEM-Easy vector; then the sequencing result was analyzed with bioinformatics methods. [Result] Whole length of the P23 gene from Theileria sergenti is 684 bp containing a 672 bp open reading frame. The deduced amino acid sequence (223 amino acid residues) contains a signal peptide of 19 amino acid residues and two fragments of transmembrane domains, with relative molecular weight of the 25.886 kD and with the pI of 9.22. The homology between the yielded sequence and Chitose of Theileria sergenti P23 gene(TS-Chitose type, D84446), Ikeda of Theileria sergenti P23 gene(TS-Ikeda type, D84447) reached 99% and 90%, respectively. The sequence has been accessed in GenBank(EU573168). [Conclusion] The protein encoded by the P23 gene has better stability and immunogenicity, thus can be used as the antigen candidate for preparing genetic engineering vaccine for Theileria sergenti. 相似文献
5.
低叶绿素b水稻突变体的抗氧化酶系统研究(英文) 总被引:9,自引:2,他引:7
[Objective] The mitigative effect of antioxidase system of a rice mutant with low chlorophyll b on photooxidative damage was studied.[Method] A rice mutant with low chlorophyll b and its wild type were taken as experimental materials to comparatively research their peroxide (H2O2) contents, the activity and isozymes of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in chloroplast.[Result] Compared with the wild type, there were many kinds of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant, and the activity of SOD, POD and CAT isozymes in leaf cells and chloroplast cell of mutant was also correspondingly higher. Under intense light condition, the H2O2 content of chloroplast in mutant was less than that in the wild type. [Conclusion] The higher activity of scavenging active oxygen can relieve the photooxidative damage made by excessive light energy of intense light on photosynthetic membrane, which is an important reason for higher photosystem Ⅱ (PS II) stability of this mutant. 相似文献
6.
[Objective] This study was to use reverse genic male sterile FHS better in field production. [Method] Low energy nitrogen ion beam was taken as a mutation source to conduct mutagenic treatment for indica rice 99-02 and the mutant FHS with special fertility that was isolated from their offspring. Some characteristics such as flowering habit and stigma exsertion rate of FHS were observed in this experiment. [Result] The reverse genic sterile rice FHS had an obvious peak flowering stage from 10:00 to 10:30, while the second peak flowering was from August 5 to August 10. Compared with PA64S, FHS flowered early and its flowering time was concentrated, showing that it is for seed propagation. The stigma exsertion rate of FHS was 85.8% and low exsertion rate was good for the purity of seed. [Conclusion] The reverse genic sterile rice FHS had good value in use, besides, it could also be used as comparison material for studying fertility alternation mechanism of photoperiod sensitive genic male sterile rice. 相似文献
7.
[Objective] The insecticidal and antibacterial bioactivity of Wikstroemia chamaedaphne Meissn were screened and bioactive substances in it were separated and purified. [Method]The Wikstroemia chamaedaphne Meissn was conducted ultrasonic extraction in petroleum ether, ethyl acetate and methanol. The insecticidal activity of Wikstroemia chamaedaphne Meissn to Mythimna separata walker and aphid were determined. The antibacterial activity of Wikstroemia chamaedaphne Meissn to Fusarium graminearu, Glomerella cingulata, F.oxysporium f.sp niveum, Alternaria solani and Fusarium oxysporium were also determined. The bioactivity-guided methods such as opencolumn chromatography and Pre-HPLC method were used to separate active components in petroleum ether extract from Wikstroemia chamaedaphne Meissn. [Result] When the concentration was 500 mg/L, 3 kinds of extracts from Wikstroemia chamaedaphne Meissn didn’t show obvious antibacterial bioactivity to 5 kinds of test samples. When the concentration was 5%, petroleum ether extract show certain topical toxicity to aphids. The ethyl acetate extract showed certain antifeedant activity to 3rd instar Larvae of Mythimna separata Walker. The fraction F4 of petroleum ether extract possessed highest topical toxicity to aphids and the lethality was 60.00%.[Conclusion] Wikstroemia chamaedaphne Meissn contained many insecticidal constituents whose active parts and mechanism were needed further researches. 相似文献
8.
In order to develop an efficient method about protein extraction which is suitable for apple proteomic analysis, four protocols of total protein extraction from apple leaves, which are trichloroacetic acid/acetone precipitation method (TCA), phenol extraction methanol/ammonium acetate precipitation method, Tris-HCl extraction method and modified Tris-HCl extraction method were compared. The results showed that the modified Tris-HCl extraction method was the most suitable method in protein extraction for two-dimensional electrophoresis (2-DE) from apple leaves based on the highest resolution and more informative spots of 2-DE gels with no apparent vertical or horizontal streaking. 相似文献
9.
华山松疱锈病的重寄生真菌(深绿木霉)中几丁质酶基因cDNA片段的克隆(英文) 总被引:2,自引:0,他引:2
[Objective] The aim of this study was to isolate chitinase gene from Trichoderma atroviride strain SS003. [Method] With the aeciospore wall of armandii pine blister rust as inducer, chitinase gene was induced to express in Trichoderma atroviride cells. The cDNA fragment of chitinase gene was cloned by RT-PCR approach. [Result] The activity of chitinase induced reached 40.17 μg/10 min; and the specific fragment amplified was 834 bp in length and proved to be the fragment of chitinase gene by sequencing and sequence analysis. [Conclusion] The result showed the feasibility of isolating the full length of chitinase gene and its transformation, and further producing chitinase. 相似文献
10.
WANG Dan-mei REN Da-ming LI Xiu-na SHI Jiao College of Biological Science Technology Shenyang Agricultural University Shenyang 《(《农业科学与技术》)编辑部》2008,(1)
[Objective] The purpose was to study the optimum conditions of Pycnoporus sanguineus for producing ligninase.[Method] A strain of lignin-degrading white-rot fungus was selected from 5 strains of collected fungi and its ligninase production and the optimum conditions for producing ligninolytic enzyme were measured.[Result]It could produce two kinds of ligninase with good thermal stability. Different temperatures, carbon sources, nitrogen sources,acidities, as well as the additions of surfactant had distinct influence on the development of lignin-degrading enzymes of the fungus. The optimum condition was drawn out: 38 ℃, pH=4.5, 10.0 g/L glucose, 1.0 g/L tartaric acid ammonium.[Conclusion] The aim of research was to provide a basis for lignin degradation in practical production. 相似文献
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[目的]全面分析不同花色杜鹃花的色素成分与稳定性。[方法]以4种不同花色的杜鹃花花瓣(浅红、紫色、白色和粉红色)为试材,对其花色素提取液进行紫外-可见光光谱扫描检测、特征显色反应和稳定性分析。[结果]试验表明,杜鹃不同花色色素不含叶绿素和类胡萝卜素;粉色、浅红色和紫色杜鹃花色素主要由花色素苷和类黄酮化合物组成;白色杜鹃花色素则含非红色的类黄酮化合物以及其他化合物,其花色素不具备邻二酚羟基或邻三酚羟基结构,揭示了杜鹃花色呈现差异的内在本质。紫外-可见光光谱扫描的结果表明,不同花色素溶液中的花色素种类与含量不同。稳定性研究表明,食品添加剂、低价金属离子对色素色泽无不良影响,但碱性环境、氧化还原剂以及Fe3+、Al3+均对其稳定性影响较大,探明了有利于杜鹃花色素稳定的环境条件。[结论]研究可为杜鹃花色素的分离鉴定奠定基础,同时也为天然杜鹃花色素的科学利用提供理论依据。 相似文献
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微胶囊化喀什小檗花色素抗氧化特性研究 总被引:1,自引:0,他引:1
[目的]研究喀什小檗花色素及其喷雾干燥制取微胶囊的抗氧化活性,以期为工业中应用微胶囊花色素提供一定的指导。[方法]采用DPPH自由基清除法和ABTS自由基清除法测定体外抗氧化活性,研究花色素在不同状态下清除DPPH自由基和ABTS自由基的作用。[结果]不同样品对DPPH自由基的清除能力依次为花色素〉微胶囊花色素;不同样品对ABTS自由基的清除能力依次为花色素〉微胶囊花色素。花色素微胶囊化前后对DPPH自由基和ABTS自由基均有一定的清除效果,但均低于维生素E的清除能力。[结论]花色素抗氧化能力较好,微胶囊花色素因为微胶囊化后花色素含量降低,协同作用降低,所以其抗氧化能力有一定程度的降低,但是花色素微胶囊化后便于贮存,由于花色素被包埋而隔离外界环境,延长了保存时间。 相似文献
15.
[目的]建立高山杜鹃的ISSR—PCR反应体系。[方法]运用正交试验分析模板DNA、TaqDNA聚合酶、Primer、Mg^2+和dNTPs5个因子对杜鹃ISSR-PCR反应影响,建立高山杜鹃ISSR-PCR反应体系。[结果]杜鹃ISSR—PCR25m反应体系中5个因子较适水平为:DNA模板浓度为1.6ng/μl,TaqDNA聚合酶浓度为0.040U/μl,Primer浓度为0.64mmol/L,dNTPs浓度为0.68mmol/L,Mg^2+浓度为2.08mmol/L。[结论]研究结果为利用ISSR分子标记技术研究杜鹃提供了理论支持。 相似文献
16.
[目的]研究黑莓果酒发酵过程中功能性成分的变化规律。[方法]以黑莓原汁复合20%梨汁为原料酿造黑莓果酒,跟踪考察黑莓果酒发酵过程中总糖、pH、单宁、总酚和花青素含量的变化。[结果]试验表明,至发酵结束黑莓果酒总糖降至约5g/L;pH主发酵期间在3.50~3.45范围内波动,陈酿期间降低至3.35;单宁在主发酵期间呈上升趋势,陈酿期间略有降低,陈酿180d后单宁含量稳定在5.58g/L;总酚在主发酵期间呈缓慢下降趋势,陈酿180d后总酚含量为0.88g/L;花青素在发酵前2d迅速下降,第3天起下降速度变慢,陈酿期间花青素含量呈缓慢下降趋势。[结论]研究可为酿造优质的黑莓果酒提供科学依据。 相似文献
17.
海南杜鹃扦插繁殖技术初探 总被引:1,自引:0,他引:1
[目的]研究海南杜鹃最佳扦插繁殖技术。[方法]以NAA和IBA为生长调节剂,于2009~2010年对海南杜鹃进行嫩枝扦插试验。[结果]在冬季扦插中,以600 mg/L NAA处理的生根率最高,为86.7%;以800 mg/L NAA处理的平均根长最长,为37.1 mm。在春季扦插中,以400 mg/L IBA处理的生根率最高,为75.0%;以800 mg/L NAA处理的平均根长最长,为40.3 mm。[结论]扦插季节对海南杜鹃扦插生根率的影响不显著,该研究结果为海南杜鹃的实际生产提供了参考。 相似文献
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[目的]研究云锦杜鹃组织培养再生植株的最佳培养基配方条件,为其快速繁殖及体细胞杂交再生植株提供基础技术参考。[方法]以云锦杜鹃幼叶为材料,接种在MS、WMP、6,7-V3种基本培养基以及不同的激素组合、培养基不同磷素用量等各种处理中,重复4次进行离体培养,筛选其脱分化和再分化效果。[结果]发现WMP有利于诱导叶出愈伤,不定芽分化最佳的基本培养基为6,7-V;继代和增殖培养基为6,7-V+0.5mg/LZT+0.1mg/LNAA+0.1mg/LIAA,平均增殖系数可达12.25;试管苗最适生根培养基为1/26,7-V+0.1mg/LNAA+0.1mg/LIAA,30d的平均生根率达到91.20%。研究还发现,在培养基中提高磷素用量有利于再生芽的伸长。[结论]6,7-V培养基在云锦杜鹃幼叶不定芽发生中起显著作用,该研究建立了最适的云锦杜鹃离体再生体系。 相似文献