| 牛皮杜鹃RAPD反应体系的优化 |
| |
| 引用本文： | 宫宇,刘宪虎,李美善,许明子,张春影,吴琼.牛皮杜鹃RAPD反应体系的优化[J].安徽农业科学,2010,38(12):6134-6135,6196. |
| |
| 作者姓名： | 宫宇 刘宪虎 李美善 许明子 张春影 吴琼 |
| |
| 作者单位： | 延边大学农学院农学系,吉林龙井,133400;延边大学农学院农学系,吉林龙井,133400;延边大学农学院农学系,吉林龙井,133400;延边大学农学院农学系,吉林龙井,133400;延边大学农学院农学系,吉林龙井,133400;延边大学农学院农学系,吉林龙井,133400 |
| |
| 基金项目： | 延边大学研究生创新基金 |
| |
| 摘 要： | 目的]建立牛皮杜鹃RAPD反应体系。方法]以野生牛皮杜鹃的叶片为材料,采用改进CTAB法提取基因组DNA,并对影响RAPD扩增反应的各因素进行优化。结果]适合牛皮杜鹃的RAPD反应体系为：在20μlPCR反应体积中加模板DNA10ng,Mg2＋浓度1.5mmol/L,引物OPC0515pmol/μl,dNTP0.15mmol/L,TaqDNA聚合酶1.0U。结论]该研究为牛皮杜鹃的遗传多样性分析提供了有益的参考。
|
| 关 键 词： | 牛皮杜鹃 RAPD 优化 |
Optimization for RAPD Reaction System of Rhododendron chrysanthum Pall |
| |
| Institution: | GONG Yu et al(Department of Agronomy,Agricultural College of Yanbian University,Longjing,Jilin 133400) |
| |
| Abstract: | Objective] To establish a RAPD reaction system of Rhododendron chrysanthum Pall.Method] leaves of Rhododendron chrysanthum Pall were used as experimental materials.Genomic DNA was extracted by the improved CTAB method,and the various factors affecting the RAPD amplification were also optimized.Results] The RAPD program of Rhododendron Chrysanthum Pall in the 20 μl PCR reaction solution were as follows:template DNA 10 ng,Mg2+ 1.5 mmol/L,OPC05 primer 15 pmol/μl,dNTP 0.15 mmol/L,Taq DNA polymerase 1.0 U.Conclusion] This study provides a useful reference for analyzing the genetic diversity of Rhododendron chrysanthum Pall. |
| |
| Keywords: | RAPD |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
