Cultivar Specific Response of CO2 Fertilization on Two Tropical Mung Bean (Vigna radiata L.) Cultivars: ROS Generation,Antioxidant Status,Physiology, Growth,Yield and Seed Quality

Increasing atmospheric carbon dioxide concentration (CO₂) is an important component of global climate change that will have a significant impact on the productivity of crop plants. In recent years, growth and yield of agricultural crop plants have been shown to increase with elevated CO₂ (EC) and have enticed considerable interest due to variation in the response of crop plants. In this study, comparative response of two mung bean cultivars (HUM‐2 and HUM‐6) was evaluated against EC at different growth stages under near‐natural conditions for two consecutive years. The plants were grown in ambient as well as EC (700 ppm) in specially designed open‐top chambers. Under elevated CO₂, marked down‐regulation of reactive oxygen species (ROS) levels, membrane disruption and activities of superoxide dismutase and catalase were noticed in both the cultivars, but the extent of reduction was more in HUM‐6. As compared to ambient CO₂, EC increased total chlorophyll, photosynthetic rate, growth and yield parameters. Cultivar‐specific response was noticed as HUM‐6 showed higher increase in yield attributes than HUM‐2. Under CO₂ treatment, soluble protein and reducing sugars decreased while total soluble sugars and starch showed an opposite trend. Principal component analysis showed that both the cultivars responded more or less similarly to EC in their respective groupings of physiological and growth parameters, but the magnitude of ROS and antioxidative enzymes was variable. The experimental findings depict that both the cultivars of mung bean showed contrasting response against EC and paved the way for selecting the suitable cultivar having higher productivity in a future high‐CO₂ environment.     

重组白细胞介素-2高效纯化方法建立

依次通过离子交换法、凝胶过滤法纯化重组白细胞介素-2(IL-2)粗品,利用SDS-PAGE电泳和hIL-2 Elisa kit检测纯化后重组IL-2的纯度和浓度。结果表明,纯化后的重组IL-2纯度和浓度高。本纯化方法简单、稳定、纯化效果好,可为重组IL-2的生产工艺提供技术支持和依据。     

野生动物非法交易犯罪案件信息特征分析

对野生动物非法交易案件进行信息特征分析,可以把握案件的发生规律,提供决策者参考,以便有针对性预防和打击犯罪,更好地保护野生动物资源。本研究应用大数据分析的方法,对野生动物非法交易犯罪案件的文本数据进行结构化处理,建立了word2vec模型。从犯罪因素的角度统计分析了本类案件的信息特征,包括侵害人的特征、作案行为特征、侵害的动物或动物制品、侵害的时间和案件发生的地点等。在梳理基本特征的基础上应用关联算法构建了关联规则。根据特征分析和关联规则,提出了开展季节性防控、对特定群体和场所进行有针对性预防、对上游下游犯罪进行联动打击、与网管等多部门合作,运用现代科技成果进行打击和防范的对策建议。     

广西主要动物源性产品中磺胺二甲嘧啶残留调查与分析

为了解广西动物源性产品中磺胺二甲嘧啶（SM₂）残留情况，本研究采用磺胺二甲嘧啶化学发光酶免疫分析法（SM₂-CLEIA）对2013-2017年采集的4 637份动物源性产品进行检测，共检出超标样品122份，涉及鲜乳、猪肉、水产品和猪尿，其中超标猪尿92份，SM₂含量100.51~860.44 μg/kg，猪尿中抗生素残留会污染水体、土壤，再通过动植物的富集作用进入食物链，危害人类健康，应引起重视；而本次受检的水牛奶、鸡肝脏、鸭肝脏、鸭肉均合格。所有受检样品超标率逐年下降，同时规模化养殖场样品合格率高于农村散养户、超市高于农贸市场。不同年份超标样品含量以2014年最高。采用食品安全指数（IFS）对广西动物源性食品中SM₂残留风险进行评估，结果均远小于1，说明在广西地区，现有的SM₂残留对人体的潜在危害较低。     

H3N2亚型猪流感病毒HA和HA1蛋白的原核表达

为原核表达H3N2亚型猪流感病毒(SIV)的HA和HA1蛋白,通过RT-PCR方法获得SIV的HA和HA1基因,克隆至原核表达载体pMAL-c5X,并转化至原核表达菌Rosetta(DE3)中,表达菌经IPTG诱导表达后进行SDS-PAGE和Western blot分析。结果显示,成功表达了H3亚型SIV的HA和HA1蛋白,目的蛋白均以可溶性蛋白和包涵体两种形式存在,并与H3N2亚型SIV的HA单克隆抗体反应,说明HA和HA1蛋白具有良好的反应原性。     

钼镉联合诱导对麻鸭肾功能及肾Bcl-2基因mRNA表达的影响

旨在研究钼镉联合诱导对麻鸭肾功能及肾脏中Bcl-2基因mRNA表达量的影响。选用360羽健康的11日龄"江南2号"麻鸭并随机分成6组,每组60只(公母各30羽)。(NH_4)_6Mo_7O_(24)·4H_2O和3CdSO_4·8H_2O分别作为本试验的钼源和镉源,在每千克基础日粮中添加不同剂量的Mo、Cd,各组的处理情况分别为:对照组(Mo 0 mg,Cd 0mg)、低钼组(Mo 15 mg,Cd 0 mg)、高钼组(Mo 100 mg,Cd 0 mg)、镉组(Mo 0 mg,Cd 4 mg)、低钼镉组(Mo 15mg,Cd 4mg)、高钼镉组(Mo 100mg,Cd 4mg)。试验期120d。在试验期第30、60、90、120天时采集血样分离血清用于检测血清中Cr、UA、BUN、Na~+、K~+、Cl-、Mg~(2+)的含量;于60、120d采集鸭肾组织检测Bcl-2 mRNA的表达量。结果显示,120d高钼镉组Cr和BUN显著高于对照组(P0.05);120d试验组血清中UA低于对照组但差异不显著(P0.05);120d试验组血清Na~+、Cl-显著低于对照组(P0.05);试验组血清K~+低于对照组但差异不显著(P0.05);90、120d高钼组和高钼镉组的血清Mg~(2+)显著低于对照组(P0.05);在60、120d,除低钼组外,其余试验组鸭肾中Bcl-2mRNA表达量均极显著低于对照组(P0.01),高钼镉组极显著高于其他组(P0.01);钼镉联合组变化比单独组更显著。此结果表明钼、镉及其联合诱导可导致麻鸭肾功能损伤、肾脏中Bcl-2 mRNA表达量下调,且钼镉呈协同作用。     

纳米TiO_2对鸡肉源微生物的光催化抑菌效果研究

[目的]研究不同作用因素对纳米TiO_2的光催化活性影响,为纳米TiO_2光催化杀菌技术的开发与应用提供参考。[方法]以菌液初始浓度、光照强度和纳米TiO_2浓度为主要变量因素,研究不同因素条件下纳米TiO_2对鸡肉源微生物P.fluorescens和M.caseolyticus的光催化抑菌作用效果。[结果]纳米TiO_2浓度增加对P.fluorescens和M.caseolyticus的抑菌作用呈先升高后降低的趋势,最佳浓度值为0.4 g/L;增高光照强度,降低初始菌液浓度,纳米TiO_2的光催化抑菌效果增强;相同试验条件下,纳米TiO_2对P.fluorescens和M.caseolyticus的光催化抑菌作用一致,对M.caseolyticus的抑菌率始终低于P.fluorescens。[结论]M.caseolyticus对纳米TiO_2的光催化作用比P.fluorescens更加敏感。     

传染性法氏囊病病毒主要结构蛋白VP2、VP1和VP2-VP1串联基因的原核表达及其初步应用

为获得传染性法氏囊病病毒(IBDV)特异性抗体检测用抗原VP2、VP1及VP2-VP1蛋白,分别设计引物扩增IBDV野毒株NN1172的VP2和VP1基因,并扩增VP2和VP1基因中抗原性和亲水性较好的重要区域,通过PCR扩增基因串联方法对截短的VP2和截短的VP1基因进行串联,首次获得VP2-VP1串联基因,并对VP2、VP1和VP2-VP1串联基因进行了原核表达和鉴定。结果成功构建了原核表达载体pET-VP2、pET-VP1和pET-VP2-VP1;诱导表达条件显示,3个重组质粒分别转入BL21菌株后经0.05 mmol/L IPTG诱导表达,分别得到分子量为69、114和63 kDa的VP2、VP1和VP2-VP1重组蛋白,且均以包涵体形式表达,3个重组蛋白分别于诱导后5、3和6 h时表达量最多。Western blot结果显示,表达的VP2、VP1和VP2-VP1蛋白与鸡抗IBDV阳性血清均具有良好的反应原性。以纯化的VP2、VP1和VP2-VP1蛋白作为包被抗原对传染性支气管炎病毒(IBV)、呼肠孤病毒(ReoV)、禽白血病病毒(ALV)和新城疫病毒(NDV)4种阳性血清检测均为阴性,表明所获得的纯化蛋白具有高度的特异性;对免疫了IBD灭活疫苗,IBD基因工程疫苗和IBD弱毒疫苗的商业鸡群进行抗体检测,结果均能显示疫苗免疫后机体抗体水平的变化趋势。本研究表明利用该原核表达系统所表达的3个蛋白均具有良好的免疫反应活性,为IBDV特异性抗体的检测和新型亚单位疫苗的研发奠定基础。     

Periplaneta americana peptide decreases apoptosis of pig-ovary granulosa cells induced by H2O2 through FoxO1

Oxidative stress can induce apoptosis of granulosa cells and lead to follicular atresia, thereby reducing the number of pigs giving birth. The aim of this study was to investigate the protective effect of Periplaneta americana peptide (PAP) on the apoptosis of the granulosa cells of pig ovaries (PGCs) induced by hydrogen peroxide (H₂O₂) via FoxO1. PGCs were treated with H₂O₂ to establish a cell apoptosis model. Cell viability was measured using the cell counting kit-8 (CCK-8) assay, and cell apoptosis was detected using flow cytometry. The malondialdehyde (MDA) level and nitric oxide (NO) content were detected to reflect the oxidative stress. Western blotting, qRT-PCR and overexpression were undertaken to determine the expression of FoxO1 and caspase-3, and immunofluorescence was used to detect FoxO1 in the nucleus and cytoplasm. PGCs were treated with 100 μM H₂O₂ for 6 hr, which resulted in oxidative damage and apoptosis and an apoptosis rate for PGCs of 32.95%. Next, PGCs were treated with 400 μg/ml PAP for 24 hr to repair the apoptosis induced by H₂O₂. PAP improved cell viability in H₂O₂-stimulated PGCs, the increased MDA level and NO content caused by H₂O₂ stimulation were reversed and the apoptotic rate of PGCs was reduced. The qRT-PCR and Western blotting results indicated that PAP decreased the H₂O₂-induced apoptosis and the expression of FoxO1 and caspase-3 in PGCs. The effect of PAP was the same following FoxO1 overexpression. FoxO1 was expressed in the nucleus when stimulated by H₂O₂ or overexpression; however, it migrated to the cytoplasm following PAP treatment. PAP decreased the apoptosis of PGCs induced by H₂O₂ by regulating FoxO1 expression and nuclear translocation.     

Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis

IntroductionCystic echinococcosis (CE) is a chronic zoonotic disease caused by the larval stage of Echinococcus granulosus (E. granulosus), which affects domestic and wild carnivores as the definitive host and ungulates as intermediate hosts. In intermediate hosts, both Th1 and Th2 cells are involved in the immune responses to an echinoccocal infection. This study aimed to investigate production of IL-4, IL-10, and IFN-γ cytokines in peripheral blood mononuclear cells (PBMCs) of CE patients before and after surgical treatment.MethodsTo evaluate cytokine production in response to E. granulosus antigens, we investigated IL-4, IL-10, and IFN-γ production in PBMCs of 20 CE patients in response to hydatid cyst fluid antigen (HCF-Ag) before and after surgical treatment using ELISA.ResultsThe mean IL-4 production from HCF-Ag stimulated PBMCs was significantly decreased (p < 0.05), while IFN-γ was significantly increased in HCF-Ag stimulated PBMCs in patients after surgery (p = 0.005).Furthermore, our results showed that there is no significant difference between IL-10 production in patients before and after treatment (p = 0.562).ConclusionsOur data Indicated production of IL-4 in cultured PBMCs of CE patients stimulated with HCF-Ag was decreased significantly. While, production of IFN-γ was increased significantly in responses to HCF Ag after surgery. We concluded that the evaluation of IL-4 and IFN-γ in HCF-Ag stimulated PBMCs of CE patients should be considered as a useful marker in the follow up of patients with cystic echinococcosis.