| H3N2亚型猪流感病毒HA和HA1蛋白的原核表达 |
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| 引用本文： | 纪方晓,陈济铛,石庆伟,王衡,张桂红.H3N2亚型猪流感病毒HA和HA1蛋白的原核表达[J].动物医学进展,2016(6):22-25. |
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| 作者姓名： | 纪方晓 陈济铛 石庆伟 王衡 张桂红 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 基金项目： | 现代农业产业技术体系建设专项资金项目(CARS-36) |
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| 摘 要： | 为原核表达H3N2亚型猪流感病毒(SIV)的HA和HA1蛋白,通过RT-PCR方法获得SIV的HA和HA1基因,克隆至原核表达载体pMAL-c5X,并转化至原核表达菌Rosetta(DE3)中,表达菌经IPTG诱导表达后进行SDS-PAGE和Western blot分析。结果显示,成功表达了H3亚型SIV的HA和HA1蛋白,目的蛋白均以可溶性蛋白和包涵体两种形式存在,并与H3N2亚型SIV的HA单克隆抗体反应,说明HA和HA1蛋白具有良好的反应原性。
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| 关 键 词： | H3N2亚型猪流感病毒 HA蛋白 HA1蛋白 原核表达 |
Prokaryotic Expression of HA and HA1 Genes of H3N2 Swine Influenza Virus |
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| Abstract: | To express the HA and HA1 proteins of H3N2 swine influenza virus,the HA and HA1 genes were amplified by RT-PCR and cloned into prokaryotic expression vector pMAL-c5X.Recombinant plasmid pMAL-c5X-HA and pMAL-c5X-HA1 were transformed to E.coli Rosetta (DE3)and induced with IPTG. The HA and HA1 proteins were expressed successfully in the forms of soluble proteins and inclusion bodies.What′s more,both of the HA and HA1 proteins could be recognized by HA antibody of H3N2 sub-type,it revealed that the HA and HA1 proteins have good antigenicity. |
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| Keywords: | H3N2 swine influenza virus HA protein HA1 protein prokaryotic expression |
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