| 重组白细胞介素-2高效纯化方法建立 |
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| 引用本文： | 王 坤,等. 重组白细胞介素-2高效纯化方法建立[J]. 特产研究, 2014, 0(1): 30-34 |
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| 作者姓名： | 王 坤 等 |
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| 作者单位： | 中国农业科学院特产研究所,长春130112 |
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| 基金项目： | 吉林省科技厅项目（20130102080JC） |
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| 摘 要： | 依次通过离子交换法、凝胶过滤法纯化重组白细胞介素-2(IL-2)粗品,利用SDS-PAGE电泳和hIL-2 Elisa kit检测纯化后重组IL-2的纯度和浓度。结果表明,纯化后的重组IL-2纯度和浓度高。本纯化方法简单、稳定、纯化效果好,可为重组IL-2的生产工艺提供技术支持和依据。
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| 关 键 词： | 重组白细胞介素-2 离子交换法 凝胶过滤法 纯化策略 |
Establishing Efficient Purification Method of Recombinant Interleukin- 2 |
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| Affiliation: | WANG Kun, LI Hai- tao, ZHANG Run- xiang, ZHU Yan- zhu, LIU Yan - huan, FENG Zhuo, MIAO Li - guang ( Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Changchun 130112, China) |
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| Abstract: | The sample of recombinant inlerleukin- 2wits purified by Ion exchange method and Gel filtration respectively. The purity and the concentration of RIL - 2 were determined by SDS - Page and hIL - 2 Elisa kit. The results showed that purity and the coneentration of RIL - 2 were high.This method is simple and stable, and the efficient purity of RIL - 2 can be obtained, it can provide evidence to produce RIL - 2 for industry. |
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| Keywords: | Recombinant interleukin - 2 Ion exchange method Gel filtration purified method |
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