双斑东方鲀循环水养殖水体细菌群落结构分析

利用16S rRNA高通量基因测序技术研究了双斑东方鲀(Fugu bimaculatus)循环水养殖系统养殖水体细菌种群结构特征,并比较养殖患病(溃疡症)的双斑东方鲀的水体细菌群落结构与健康的之间的差异,探讨细菌群落结构与双斑东方鲀细菌性病害发生的相关性。研究结果表明,双斑东方鲀循环水养殖水体细菌群落的优势细菌门为变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes),优势细菌纲为γ-变形菌纲(Gammaproteobacteria)、黄杆菌纲(Flavobacteriia)、α-变形菌纲(Alphaproteobacteria),优势细菌目为黄杆菌目(Flavobacteriales)、硫发菌目(Thiotrichales)、红细菌目(Rhodobacterales)、交替单胞菌目(Alteromonadales)、弧菌目(Vibrionales),优势细菌科为黄杆菌科(Flavobacteriaceae)、硫发菌科(Thiotrichaceae)、红细菌科(Rhodobacteraceae)、弧菌科(Vibrionaceae)、交替假单胞菌科(Pseudoalteromonadaceae)。患病的和健康的循环水养殖水体细菌群落种类组成相似度高,细菌群落多样性指数无较大差异,在同一分类水平上各细菌群落丰度占比差异显著;二者核心微生物(属)的差异明显,健康的循环水养殖水体核心细菌群落以Polaribacter属为主;患病的循环水养殖水体核心细菌群落以弧菌科的发光杆菌属(Photobacterium)和弧菌属(Vibrio)为主,而发光杆菌属和弧菌属的多数种类属于病原菌或条件致病菌。双斑东方鲀患病(溃疡症)与养殖水体细菌群落的弧菌属丰度占比高有关。该研究结果对双斑东方鲀循环水养殖细菌性病害的监测、预警、诊断及管理控制具有重要的意义。     

两种三疣梭子蟹(Portunus trituberculatus)养殖塘中浮游细菌的季节变化

结合基于细菌16S rRNA基因的T-RFLP技术与克隆测序技术,对象山港三疣梭子蟹、脊尾白虾混养模式下改良塘M1(塘底铺网四周铺砂)以及传统塘M2(土塘)水体不同季节细菌群落结构和多样性进行分析。结果显示,M1、M2养殖塘水体细菌群落主要由变形纲门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、蓝藻门(Cyanobacteria)组成。养殖过程中,细菌群落结构随时间发生了显著变化,不同养殖塘水体细菌群落结构变化的方向不同。M1养殖塘水体由放线菌门主导的群落结构向蓝藻菌门以及变形菌门主导的群落结构发展,M2养殖水体由蓝藻菌门主导的向变形纲门主导的群落结构发展,说明养殖后期改良塘水体有利于蓝藻细菌的生长。无论是M1还是M2,拟杆菌门在8-9月含量最高,且养殖过程中在M2水体的含量始终高于M1。随着养殖时间的推移,M1养殖塘水体细菌群落由多样性指数高的稳定性结构向多样性指数低的不稳定性结构转变,而M2水体养殖后期仍保持较高的多样性指数。PCA分析结果显示,M1养殖塘水体细菌群落差异性大于M2,说明M2水体细菌群落对环境变化的抵御能力大于M1。相关性分析结果显示,不同养殖塘水体细菌群落分布受环境的影响效应不同。     

循环水养殖系统生物滤池细菌群落的PCR-DGGE分析

通过模拟实验对循环水养殖系统中不同初始NH ₄N浓度的生物滤池中生物膜上和水中的细菌数量及群落种类组成进行了研究。对成熟生物膜及水体样品中的异养菌、氨氧化菌、亚硝酸盐氧化菌的培养计数结果表明,随着生物滤池初始氨氮浓度增大,除异养细菌数量逐渐下降外,生物膜上的氨氧化菌和亚硝酸盐氧化菌数量呈逐渐增加趋势,且均高出水样3~4个数量级;同时对上述样品的16S rRNA基因片段的PCR扩增产物进行变性梯度凝胶电泳(DGGE)分析及其序列同源性分析的结果表明,生物膜和水中都有较高的细菌多样性,同一初始氨氮浓度的滤池中生物膜上的细菌多样性高于水中的。生物滤池中的细菌主要由拟杆菌门的黄杆菌纲和变形菌门的α-、β-、γ-变形菌纲的15种细菌组成。生物膜上的优势菌包括奥雷氏菌属、湖饲养者菌属、泥滩杆菌属、沉积杆菌属、雷辛格氏菌属、冷蛇形菌属和亚硝化单胞菌属等;水体中的优势菌则有明显差异,主要有蛋黄色杆菌属、Nautella,玫瑰杆菌属和一种硫氧化菌等。初始氨氮越高的滤池中,亚硝化单胞菌属的细菌在生物膜上所占比例越高,逐渐成为优势菌之一。实验证实,挂膜初期,提高水体中初始氨氮浓度,有利于硝化细菌的富集和固着,提高生物滤池的除氮效率。     

黄岛近海岸贝类养殖区细菌群落结构多样性及与环境因子响应

为了研究近海贝类开放养殖水域中细菌种群分布规律以及微生物群落多样性对环境因子的响应及与水产动物发病的关系,本实验于2019年7—11月采集黄岛某开放养殖水域不同样点海水样本,分析不同水质因子(水温、盐度、pH、DO、叶绿素、氨氮、磷酸盐、硝酸盐和亚硝酸盐)的动态变化,并利用高通量测序的方法分析了不同月份水样和栉孔扇贝组织样本中微生物群落结构和多样性的差异,探讨了环境因子与微生物群落结构的相关性。结果显示,水样本中细菌群落结构共检测到42个门,94个纲。由变形菌门、拟杆菌门、蓝细菌门、放线菌门及厚壁菌门等组成,其中变形菌门为优势菌门,主要包括γ-变形菌纲和α-变形菌纲。不同月份,细菌OTU水平多样性分析结果为11月>9月>7月>10月>8月;弧菌OTU水平多样性分析结果显示,弧菌的多样性呈现先升高后降低的变化,与水温的变化趋势一致,8月养殖区多样性高于对照区。RDA分析表明,叶绿素含量(μg/L)和亚硝酸盐的浓度(mg/L)对门水平的物种组成和群落结构具有显著的影响。磷酸盐含量(mg/L)和DO (mg/L)对弧菌属水平的群落结构具有显著影响。扇贝组织样本细菌群落...     

凡纳滨对虾不同养殖密度高位池水体细菌群落动态

通过对养殖水体环境基因组DNA中细菌16S rRNA基因V4–V5区的高通量测序和生物信息学分析,研究了两种养殖密度的凡纳滨对虾(Litopenaeus vannamei)高位池水体中细菌群落在养殖过程中的动态。结果显示,养殖过程中各菌群相对丰度变化明显,细菌多样性随时间逐渐提高,优势菌群为变形菌门(Proteobacteria)、蓝藻门(Cyanobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和浮霉菌门(Planctomycetes)。随着养殖时间增长,蓝藻丰度所占比例逐渐减少,而变形菌、拟杆菌和浮霉菌丰度逐渐增大,同时养殖前期高密度池浮霉菌丰度显著高于低密度池(P0.01),而其他菌群无显著差异。结果表明,养殖期前50 d不同养殖密度水体细菌群落差异较大,而后30 d内细菌群落的时间异质性大于空间异质性,这意味着高位池水体菌相被划分为两类,到养殖后期菌相快速转变,养殖密度所带来的影响被减弱。     

浒苔腐烂过程中水体细菌群落结构变化的PCR-DGGE分析

通过生态模拟实验,利用分子生态学技术,对浒苔(Enteromorpha prolifera)腐烂过程中水体细菌群落结构的变化进行了研究。将3.0 kg完全死亡的浒苔置于容积为500 L的实验容器中,定期取水样,进行化学需氧量(COD)、溶解氧(DO)、好氧性异养菌数量测定及细菌种群结构的分析。实验结果表明,随着浒苔腐烂程度的增加,水中的DO呈逐渐减少趋势,COD和好氧性异养菌数量均呈先增加后减少的变化趋势;水体细菌主要由拟杆菌门黄杆菌纲和变形菌门α-、γ-、ε-变形菌纲的细菌组成,其优势菌群在浒苔腐烂不同时期有较大程度的改变,初期黄杆菌纲细菌为主要优势细菌,后期则以α-、γ-、ε-变形菌纲细菌为主要优势细菌。本研究结果为研究浒苔爆发对海洋环境的影响提供了理论依据。     

珠江河网冬季浮游细菌群落结构及其影响因素

为了解珠江河网冬季浮游细菌群落结构,探讨影响其群落结构的环境因素,于2017年11月和2018年1月在珠江河网(112.18°E～113.51°E,22.38°N～23.17°N)采集表层水样,借助IlluminaMiSeq高通量测序平台,运用16S rDNA扩增子技术研究珠江河网冬季浮游细菌群落结构,使用R(3.5.2)软件包以及SPSS进行统计分析及作图。结果表明,变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)、蓝细菌门(Cyanobacteria)是珠江河网丰度较高的门类,细菌群落优势种群依次为γ-变形菌纲(γ-Proteobacteria)、放线菌纲(Actinobacteria)和α-变形菌纲(α-Proteobacteria)。珠江河网冬季浮游细菌具有较高的菌群多样性(两次样品的Shannon指数分别为6.78±0.29、6.23±0.71), NMDS分析表明,浮游细菌群落结构之间不存在显著的地域差异。Pearson相关性分析表明,温度与γ-变形菌纲(P0.05)、放线菌纲(P0.05)以及α-变形菌纲(P0.01)的丰度显著正相关,pH与放线菌纲(P0.05)、α-变形菌纲(P0.01)的丰度以及多样性指数(Shannon:P0.01; Simpson:P0.01)显著负相关,溶解氧与α-变形菌纲的丰度显著负相关(P0.05)。冗余分析(RDA)结果显示,温度、pH是影响浮游细菌群落的主要因子。     

复合池塘循环水养殖系统微生物群落结构分析

于2008年6至10月逐月调查研究了新型复合池塘循环水养殖系统各塘水体理化和微生物分子特征.通过提取水样中细菌总DNA,扩增其16S rDNA基因V3区,再经DGGE分析获得图谱,选择其中主要的13条特征条带进行克隆测序.研究结果表现为,随水流方向(Pl→P5),各循环塘溶氧(DO)和透明度(SD)依次呈明显下降趋势,而NH4~+-N,TP和COD_Mn水平呈明显上升趋势;与对照塘相比,循环塘DO和SD升高,COD_Mn和营养盐水平降低.DGGE图谱分析显示,各养殖塘微生物种类丰富,循环塘与对照塘的群落结构存在明显差异,且差异主要表现在较弱的条带上;13条特征条带的测序结果表明,池塘优势菌群分属4个门:放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、蓝细菌门(Cyanobacteria)和拟杆菌门(Bacteroidetes),其中有2条属于蓝细菌门的条带特定分布于对照塘.典型对应分析(CCA)排序结果显示,DGGE图谱条带分布与理化因子密切相关.与传统池塘养殖相比,该养殖模式有助于改善养殖池塘微生态环境.本研究旨为池塘微生物群落结构及其功能关系研究提供借鉴.     

中华绒螯蟹生态育苗池塘细菌群落分析

为探究江苏省射阳地区沿海滩涂中华绒螯蟹(Eriocheir sinensis)生态育苗池塘细菌群落结构,分别对养殖池塘水体(S7,S8,S9)和池塘底泥(N7,N8,N9)细菌群落16SrDNA的V3、V4、V5可变区进行扩增,检测合格的文库进行高通量测序;利用Qiime(v1.7)软件,对序列阈值97%相似性水平进行聚类分析;分别以门、纲、目、科和属的分类水平对样本进行α-多样性指数分析;以Chaol指数和Shannon指数评估菌种丰富度和多样性。结果表明,3个生态育苗池塘得到OTU分别为8 881、7 406、8 566个。S7微生物归属于16门、29纲、58目、97科、191属;S8为9门、18纲、38目、68科、132属;S9为17门、32纲、60目、111科、227属。N7为41门、103纲、198目、325科、572属;N8为42门、103纲、203目、318科、534属;N9为40门、99纲、196目、323科、578属。养殖水体优势菌群以变形菌门(Proteobacteria)为主,其次是拟杆菌门(Bacteroidetes);池塘底泥优势菌群以变形菌门为主,其次为拟杆菌门、绿弯菌门(Chloroflexi)和浮霉菌门(Planctomycetes)。养殖池塘底泥中细菌群落的丰富度和物种多样性明显高于养殖水体,水体中细菌群落的丰富度S9S7S8,物种的多样性S7S9S8。池塘底泥中细菌群落的丰富度N7N9N8,物种的多样性N7 N9N8。     

节能型半滑舌鳎循环水养殖车间优化设计

优化设计建设了半滑舌鳎循环水养殖车间,车间建筑面积2 880 m2,养殖水面1 340 m2.优化设计主要包括节能型养鱼车间、循环水处理工艺、补充水及外排废水处理工艺、养鱼池、生物滤池及渠道式紫外线消毒池设计.该养鱼车间比一般低拱车间节能30%以上.生物滤池为4段流水式结构.养鱼池能自动清污,倒U形管控制水位.渠道式紫外线消毒池消毒效率高,细菌去除率99%以上.通过养鱼工程优化设计及基地建设,提出了我国未来海水循环水养殖的三级推广模式.     

Comparison of bacterial communities in channel catfish Ictalurus punctatus culture ponds of an industrial ecological purification recirculating aquaculture system

The industrial aquaculture pond system has gradually replaced the use of traditional earthen pond, as it causes less pollution and is more economical. In this study, an industrial ecological purification recirculating aquaculture system consisting of the water source pond, high‐density culture ponds, a deposit pond, and ecological purification ponds for channel catfish cultivation was established. Twelve water samples from different ponds were sequenced, and the bacterial communities were analysed. The abundances of Cyanobacteria and Merismopedia varied in different functional ponds of the system. The water quality was stable after two months of cultivation at 1.89 ± 0.22 mg/L total nitrogen, 1.1 ± 0.08 mg/L NH₄‐N and 0.43 ± 0.1 mg/L total phosphorus. The fish weight increased in a nearly linear manner, reaching 237.63 ± 23.8 per fish at day 120. An analysis of the environmental parameters, water quality and fish weight suggested that the system had an effective water purification process. Canonical correspondence analysis showed that the community was affected at the genus and phylum levels by different environmental parameters. We identified several dominant beneficial bacteria with nutrient removal abilities. Overall, our results demonstrated that the ecological purification recirculating aquaculture system had notable effects on water quality improvement and promoted changes in bacterial populations. These results provide important information on the microbial ecology of pond industrial eco‐aquaculture systems.     

Microbial communities in sea cucumber (Apostichopus japonicus) culture pond and the effects of environmental factors

This study investigated microbial community composition as well as their correlation with environmental factors of Apostichopus japonicus culture ponds in northern China by 16S rRNA gene amplicon sequencing. The results showed that microbiota richness varied consistently with diversity in the pond ecosystem. Microbiota richness and diversity were highest in sediment, followed by gut of A. japonicus and water. The dominant bacterial phylum in the pond ecosystem is Proteobacteria. Gammaproteobacteriaeria and Flavobacteria are two dominant bacterial classes in the ecosystem. There is significant difference (p < 0.05) between dominant bacterial communities at the levels of order, family and genus. There is also remarkable regional difference (p < 0.05) between microbial community composition in the pond ecosystems. Specifically, microbial community composition in Changhai and Yingkou show a high similarity, so do those of Laoting and Rushan. According to the redundancy analysis of the microbial community composition and pond environmental factors, chemical oxygen demand is the dominant environmental factor determining microbial community composition in pond water; sulphide has the greatest influence on the microbial community composition in pond sediment; the rest of environmental factors have varied influence on microbial community composition in pond ecosystems.     

冻融期刺参养殖池塘沉积物菌群结构特征及其影响因素

本研究以我国北方典型岸基半开放刺参(Apostichopus japonicus)养殖池塘为对象,利用高通量测序技术构建冻融期刺参养殖池塘沉积物菌群16S rRNA基因测序文库,解析封冰期、融冰期和化冰期刺参养殖池塘沉积物菌群结构特征,并查明影响菌群结构的主导环境因子。结果显示,冻融期刺参养殖池塘沉积物菌群丰度和多样性表现为整体下调趋势,在融冰初期呈现显著性波动(P<0.05)。冻融期刺参养殖池塘沉积物菌群结构呈现显著性差异,封冰期、融冰期和化冰期差异菌群分别隶属于厚壁菌门(Firmicutes)、酸杆菌门(Acidobacteria)和软壁菌门(Tenericutes)。尽管不同阶段微生物相对丰度比例不同,但第一优势菌门均隶属于变形菌门(Proteobacteria),相对丰度高于49.04%;次优势菌门则呈现出显著性变化,其中,封冰期为拟杆菌门(Bacteroidetes),融冰期为绿弯菌门(Chloroflexi)和放线菌门(Actinobacteria),化冰期为浮霉菌门(Planctomycetes)。环境因子与菌群相关性分析表明,冻融期刺参养殖池塘沉积物菌群结构与环境因子具有显著相关性(P<0.05),温度、盐度、总氮和总有机碳是沉积物菌群的主导环境因子。本研究将为刺参养殖池塘精细化管理提供理论依据。     

微生物制剂对养殖池塘细菌群落影响的PCR-DGGE分析

为了解微生物制剂在水产养殖环境修复中的作用机制,采用聚合酶链式反应—变性梯度凝胶电泳(PCR-DGGE)方法,研究不同微生物制剂对养殖池塘上覆水和沉积物中细菌群落的影响。结果显示,施用微生物制剂前后,试验塘上覆水样品中细菌的DGGE优势条带无明显变化,细菌群落组成没有明显差异,提示微生物制剂对试验塘上覆水中的细菌群落组成影响很小。相对而言,施用过微生物制剂的池塘相对于对照池塘其细菌群落结构发生的变化较大;泼洒EM菌原粉3 000 g/hm2加芽孢杆菌原粉750 g/hm2的试验塘沉积物中细菌群落2 d内发生了较大变化,之后相对稳定,对照池塘和泼洒EM菌原粉3 000 g/hm2加光合细菌原粉1 500 g/hm2的试验塘沉积物中细菌群落组成变化很小。     

Bacterial composition,abundance and diversity in fish polyculture and mussel–fish integrated cultured ponds in China

Bacterial community and abiotic environmental parameters in twelve freshwater aquaculture ponds were analysed. According to the major component of stocked animals, the ponds were grouped into four types: black carp Mylopharyngodon piceus, largemouth bass Micropterus salmoides, yellow catfish Pelteobagrus fulvidraco and pearl mussel Hyriopsis cumingii ponds. Each type of pond was stocked with three species of Chinese carps (silver carp, bighead carp and gibel carp) to form a unique mode of fish polyculture or mussel–fish integrated culture. The bacterial composition was identified using 16S rDNA sequencing. Totally, 3701 and 11 150 operational taxonomic units (OTUs) were identified from the water and sediment samples respectively. The number of OTUs, abundance‐based coverage estimator, Chao1 index and Shannon diversity index were lower in the water column than in the sediment, suggesting that diversity and stability of bacterial community were higher in the sediment. In the water column, Proteobacteria, Actinobacteria and Bacteroidetes dominated at the phylum level, and 26 dominant genera were identified. In the sediment, Proteobacteria, Chloroflexi, Bacteroidetes, Acidobacteria and Nitrospirae dominated at the phylum level, and 25 dominant genera were identified. Bacterial compositions between the ponds with different aquaculture modes were similar at the phylum levels, but varied at the genus levels. The bacterial composition in the ponds was correlated with hardness, ammonia and total nitrogen in the water column. This study indicates that the type of aquaculture mode is a factor regulating the microbial community, which provides an insight towards microbial management through probiotic manipulation in pond culture.     

不同鱼类和采样时间的养殖池塘环境微生物群落研究

微生物群落是养殖池塘生态系统重要的组成部分。本研究在不同月份采集尼罗罗非鱼和斑点叉尾鮰池塘水体样品,分析水样中的硝酸盐氮、亚硝酸盐氮、氨氮、总氮和总磷等理化指标,利用Biolog-Eco微平板技术分析水体中微生物对各类碳源代谢的平均颜色变化率,利用高通量测序技术分析水体中的菌群结构,以期了解养殖鱼种类和季节对养殖池塘环境及菌群结构的影响。结果表明：冬季淡水养殖池塘水质和菌群结构不同于其他季节,养殖鱼类种类对池塘水体理化指标和水体微生物菌群结构的影响不大。不同采样时间的池塘水体理化指标差异显著,同一采样时间不同养殖鱼类池塘水体理化指标之间无显著差异。其中,1月尼罗罗非鱼养殖池塘水样中的NH4-N含量高于其他月份,显著高于4月和7月的水样(P<0.05)；1月TP含量显著高于1月、4月和7月(P<0.05)。1月斑点叉尾鮰养殖池塘水样中的TP和NO₃-N含量显著高于其他三个月份(P<0.05)。Biolog-ECO微平板技术检测到尼罗罗非鱼和斑点叉尾鮰养殖池塘水样中的微生物群落对碳水化合物和氨基酸的利用能力较强,对酚胺类化合物的利用能力较弱,4月斑点叉尾鮰养殖池塘水体微生物对碳源的利用率高于尼罗罗非鱼养殖池塘。高通量测序结果发现厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)是尼罗罗非鱼和斑点叉尾鮰养殖池塘水体中的绝对优势菌门。hgcI clade、CL500-29 marine group和C39等参与氮磷循环的菌属为淡水池塘养殖水体中的主要菌属。本研究结果为了解淡水养殖环境中微生物群落结构与变化规律、更有针对性的构建淡水养殖池塘尾水生态化处理方法提供科学依据。     

不同季节刺参养殖池塘沉积物菌群结构及其影响因素

本研究以辽宁省营口地区刺参(Apostichopus japonicus)养殖池塘为研究对象,利用高通量测序技术,构建不同季节刺参养殖池塘沉积物菌群16S rRNA基因测序文库,解析刺参养殖池塘沉积物菌群的季节性差异和共性,查明影响沉积物菌群构成的主导环境因子。结果显示,营口地区刺参养殖池塘沉积物菌群的丰度和多样性以夏季最高,春秋次之,冬季最低。不同季节刺参养殖池塘沉积物差异菌群呈显著性季节演替特征(P<0.05)。其中,春季差异菌群主要隶属于拟杆菌门(Bacteroides),夏季差异菌群主要隶属于酸杆菌门(Acidobacteria)、疣微菌门(Verrucomicrobia)和浮霉菌门(Planctomycetes),秋季差异菌群主要隶属于厚壁菌门(Firmicutes),冬季差异菌群主要隶属于放线菌门(Actinobacteria)。尽管不同季节沉积物细菌组分比例不同,但第一优势菌门均隶属于变形菌门(Proteobacteria)(相对丰度>43.19%)。环境因子中,影响刺参养殖池塘沉积物菌群的主导环境因子为温度、总有机碳、总氮和pH。本研究将为刺参养殖池塘微生态调控提供理论参考。     

Spatial and temporal succession of bacterial communities in three artificial fishponds

Fishponds are artificial yet dynamic environments. Microbes play a vital role in the conversion and metabolism of organic matter in aquaculture environments such as fishponds. Changes in bacterial community composition in three fishponds over a 5‐month period were investigated by sequencing the 16S rRNA gene through Illumina Miseq. A total of 2,195,879 sequences and 9,975 operational taxonomic units (OTUs) were obtained. Further analysis showed that the dominant phyla in three ponds were similar, including Proteobacteria, and Bacteroidetes. A comprehensive analysis of the diversity, redundancy analysis (RDA) and partial redundancy analysis (pRDA) showed a spatial‐temporal structural pattern in the bacterial communities. The bacterial community composition in the three fishponds was shaped more by spatial variation than by temporal variation. The effect of pond isolation was emphasized by the significant differences observed in the three fishponds. However, the temporal succession of bacterial communities in each pond exhibited a similar trend. Correlation analysis and RDA also indicated that temporal variation was significantly associated with TOC, TN and NO₂^?. This study would improve our understanding of the assembly mechanisms of a microbial structure in artificial pond ecosystems, and provide useful information for fishpond management.     

The dynamics of bacterial community in a polyculture aquaculture system of Penaeus chinensis,Rhopilema esculenta and Sinonovacula constricta

Polyculture with Chinese shrimp Penaeus chinensis, jellyfish Rhopilema esculenta and razor clam Sinonovacula constricta (S.J.R) is a common aquaculture system in Dandong, China. Investigation on the composition and function of microbial communities and their relationship with environmental factors in aquaculture systems have crucial implications. Therefore, the sequencing of 16S rRNA gene was applied to profile the bacterial community composition of water and sediment in S.J.R polyculture system in the present study. Proteobacteria was the dominant phylum in S.J.R polyculture ponds following with Actinobacteria and Bacteroidetes in water; in contrast, more diverse phyla were detected in sediments. Water temperature, pH and dissolved oxygen were determined the bacterial community composition in the S.J.R polyculture systems. Specially, Planktomarina, PS1 clade, Candidatus Aquiluna and Fibrobacteres, which can resist the phytoplankton blooms or degrade the cellulose, were more abundant in water at the early stage of cultivation in the S.J.R polyculture system. Moreover, the functions of bacterial communities in water were mainly associated with cell communication mediated, while the functions related to basal metabolism were enriched in sediments. The findings of this study allowed us to better understand the bacterial community in the S.J.R polyculture system and accumulated data for the improvement of cultural management.