共查询到20条相似文献,搜索用时 140 毫秒
1.
2.
3.
从山东省分离了6株鸡传染性鼻炎菌株,经过抗原比较,攻毒交叉保护试验,发现HPG-HZ株的保护性最佳,利用HPG-HZ株与标准A型菌株HPG-0083株和标准C型菌株HPG-Modesto株结合,成功研制了鸡传染性鼻炎多价油乳剂灭活苗。将该疫苗在山东省、河北省、河南省等地共4500万只鸡进行田间免疫试验和与同类疫苗的对比试验,结果表明,鸡传染性鼻炎多价油乳剂灭活苗对鸡群安全、有效,无副作用,免疫期可达6个月以上。特别对山东分离株的免疫保护比进口和国产同类疫苗均有优势。 相似文献
4.
从山东省分离了6株鸡传染性鼻炎菌株,经过抗原比较,我叉保护试验,发现HPG-HZ株的保护性最佳,利用HPG-HZ株与标准AG力株HPG-0083株和标准C型菌标HPG-Modesto株结合成功研制了鸡传染性鼻炎多价油乳剂灭活苗。将该疫苗在山东省、河北省、河南省等地共4500万只鸡进行田间免疫试验类疫苗的对比试验,结果表明,鸡传染性鼻炎多介油乳剂灭活苗对鸡群安全、有效,无副作用,期可达6个月以上,特 相似文献
5.
传染性鼻炎 ( IC)由鸡副嗜血杆菌引起 ,菌型分为 page血清型 A、B、C,发病鸡主要表现鼻粘膜发炎、流鼻涕、眼睑水肿和打喷嚏 ,造成部分鸡死亡和产蛋下降 ,给养鸡业带来较大的经济损失。疫苗免疫对该病控制起到良好的作用 ,原用疫苗有福尔马林灭活苗或硫柳汞灭活苗 ,但该类苗免疫期较短 ,近年来以 A型副嗜血杆菌做成的单价鼻炎油乳剂灭活苗在山东也得到较普遍应用 ,但没能得到很好地控制。为了更好地对本病进行防疫 ,研制了鸡传染性鼻炎双价油乳剂灭活苗。1 材料与方法1 .1 菌种鸡副嗜血杆菌 ,page血清型 A和 C,购自中国兽药监察所。菌… 相似文献
6.
7.
本研究采用酶联免疫吸附试验(ELISA)间接法检测鸡葡萄球菌免疫及感染后体内抗体水平的变化,此法特异性强,重复性好。运用此法对接种葡萄球菌油佐剂灭活苗和葡萄球菌氢氧化铝灭活苗的雏鸡血清抗体的动态进行检测,结果显示葡萄球菌油佐剂苗免疫后,抗体水平稳步上升,第3周达到高峰,并一直维持到第6周以后,葡萄球菌氢氧化铝灭活苗免疫后的抗体动态与之相似。文中还探讨了免疫鸡在不同免疫阶段血清抗体水平与葡萄球菌攻毒时保护率之间的关系。 相似文献
8.
《养殖与饲料.饲料世界》2015,(6)
从一鸡场患病鸡体内分离出1株鸡致病性大肠杆菌,将分离的菌株扩增、灭活,制备成油乳剂灭活苗。对灭活苗进行物理性状、安全性、保护力等检验,确认合格后,对鸡场进行免疫,效果良好。该油乳剂灭活苗对本场鸡有较好的保护作用。 相似文献
9.
在分离的6株传染性鼻炎茵株中择优选取其中4株,分别制成油乳剂灭活苗,免疫7周龄SPF鸡,免疫3周后分别用分离茵株攻毒。结果HPG-HZ株对各茵株的保护效果最好,平均保护率达76%以上,而用标准株HPG-0083株制成的疫苗,对各分离株的平均保护率只有60%。 相似文献
10.
分子筛凝胶浓缩新城疫病毒疫苗的研究 总被引:1,自引:0,他引:1
本文采用新型分了筛凝胶作为浓缩剂对新城疫毒感染鸡胚的尿囊进行了浓缩实验,结果表明,该浓缩胶可使新城疫病毒浓缩96.24倍,病毒的损失量为8.65%,用浓缩胶浓缩新城疫毒后制成灭活苗,浓缩苗进行鸡的免疫实验,以研究浓缩疫苗的免疫效果,浓缩新城疫抗原后灭活苗免疫后8天抗体水平迅速上升,HI为5.6,并持续上升,均高于对照组,说明浓缩疫苗可以使鸡产生较高的抗体水平。 相似文献
11.
An evaluation of the cross-protection afforded by inactivated infectious coryza vaccines 总被引:2,自引:0,他引:2
PJ BLACKALL 《Australian veterinary journal》1991,68(8):266-267
The cross-protection afforded by three inactivated infectious coryza vaccines was evaluated. Each vaccine contained one of the following strains of Haemophilus paragallinarum, HP31, HP60 and HP14. Strain HP31 belongs to Kume serovar C-2, strain HP60 belongs to Kume serovar C-4 while strain HP14 belongs to Kume serovar A-4. Four groups of twenty six-week-old specific-pathogen-free chickens were either given a single dose of an aluminium-hydroxide based vaccine (three groups) or left as unvaccinated controls. Three weeks after vaccination, all four groups were challenged with virulent H paragallinarum. One half of each group was challenged with the strain HP31 and the other half with strain HP60. The efficacy of the vaccines was assessed in terms of the prevention of the typical clinical signs and macroscopic lesions of infectious coryza as well as the prevention of any colonisation by the challenge organism. The serovar C-2 and serovar C-4 vaccines protected more than 50% of birds against either a serovar C-2 or C-4 challenge while the serovar A-4 vaccine protected less than 50% of birds. 相似文献
12.
鸡传染性鼻炎三价灭活疫苗安全性试验和效力检验初报 总被引:1,自引:1,他引:0
利用PageA、B、C型副鸡禽杆菌,研制鸡传染性鼻炎(IC)三价灭活疫苗。用6批IC疫苗进行了大剂量单次接种和单剂量多次接种的安全性试验,结果表明,IC三价灭活疫苗安全无副作用;用3批疫苗进行SPF鸡和普通鸡的免疫效力试验,结果对A、B、C型副鸡禽杆菌攻毒的保护率≥80%;商品鸡42日龄首免,110日龄二免,二免后约9个月对A、B、C型副鸡禽杆菌攻毒的保护率≥80%;用4℃~8℃保存一年的3批疫苗进行了SPF鸡的近期免疫效力试验,结果对A、B、C型副鸡禽杆菌攻毒的保护率≥80%。 相似文献
13.
为研制牛传染性鼻气管炎病毒(IBRV)灭活疫苗,本研究以国内分离鉴定的IBRV LN01/08株为种毒,优化病毒增殖条件,获得病毒滴度达108.0TCID50/mL,将其灭活制备疫苗.为比较不同免疫佐剂的效果,分别以矿物质白油和Montanide ISA206佐剂配制灭活疫苗,进行牛体免疫试验.临床观察和血清中和抗体检测结果表明,Montanide ISA206佐剂乳化的疫苗在降低副反应和增强免疫效果方面优于矿物质白油佐剂.应用Montanide ISA206佐剂制备3批灭活疫苗,并对其安全性和免疫保护效果进行测定.结果表明该疫苗安全可靠,对强毒攻击可产生较好的抵抗力,攻毒保护率达80%.疫苗在2℃~8℃保存12个月后仍能保持良好的免疫效果. 相似文献
14.
15.
16.
Differently formulated inactivated infectious coryza vaccines were administered to 6-week-old chickens as a single dose of 10(8) colony-forming units of Haemophilus paragallinarum HP31. After 3 weeks, all chickens were challenged by intrasinus inoculation of HP31. Two vaccines, one containing an aluminum-hydroxide adjuvant and the other a combined aluminum-hydroxide + mineral-oil adjuvant, gave the best protection (means of 80% and 90%, respectively). Two vaccines that contained mineral oil as the sole adjuvant gave less protection (50% and 35%). The Quil A vaccine gave no significant protection. Granulomatous swellings developed at the site of injection in birds given mineral-oil adjuvant but not in those that received other adjuvants. 相似文献
17.
Laboratory trials were carried out with an O2:K1 vaccine prepared with either the Freund's complete or incomplete adjuvant. Both types of vaccine administered subcutaneously were highly effective against a challenge with the vaccine strain within three to four weeks after vaccination at two to three weeks of age. The complete adjuvant vaccine was more effective than the incomplete adjuvant vaccine when administered to chickens of an earlier age, and in the rate of development and duration of immunity. The efficacy of both vaccines was unimpaired by their incorporation with the Newcastle disease oil adjuvant (inactivated) vaccine (Newcadin). The use of an oil adjuvant vaccine was not found to affect the rate of growth adversely or to produce any other reaction prejudicial to its commerical application. The efficacy of the vaccines was unimpaired by their incorporation with Newcastle disease oil adjuvant (inactivated) vaccine (Newcadin) thus demonstrating the possibility of producing a combined Escherichia coli/Newcastle disease virus vaccine. 相似文献
18.
M M Rweyemamu O Umehara D de Lucca-Neto M C Baltazar F E Vicente R da R Medeiros-Neto 《American journal of veterinary research》1986,47(6):1243-1248
Avridine, a lipoidal amine with interferon-inducing and adjuvant properties, was an effective adjuvant for Newcastle disease antigen (NDA) in chickens. Eleven vaccine lots were evaluated: 2 commercial water-in-oil vaccines, 4 experimental oil emulsion vaccines, 4 avridine-containing vaccines, and a control lot of nonadjuvanted antigen. Avridine significantly enhanced the immunologic responses of chickens against NDA. Chickens vaccinated with the avridine-containing vaccines had significantly higher antibody titers (hemagglutination inhibition) than did chickens vaccinated with the commercial vaccines. Experimental oil emulsion vaccines prepared from the same antigens as avridine-adjuvanted vaccines induced higher hemagglutination inhibition antibody titers after primary but not after booster vaccination. Use of avridine as an adjuvant for NDA in vaccines for chickens induced immunologic protection rates similar to those induced by oil emulsion vaccines, without causing the reactogenic and tissue residue problems associated with the use of oil vaccines in chickens. 相似文献
19.
应用3批鸡传染性鼻炎二价油乳剂灭活疫苗进行了保存期检验,结果表明,二价苗在4-8℃保存12个月和18个月后免疫鸡群,鸡群对A、C型强致病力菌株的保护率在二免后4周左右分别为100%和88.5%,血清SPA、阻断ELISA和HI抗体阳性检出率较高,但疫苗保存18个月后所免疫鸡的血清C型HI抗体效价的几何平均数有显著的下降。因此,该二价苗的保存期以12个月为宜。 相似文献
20.
为筛选出理想的鸭TMUV-AIV(H9亚型)二联灭活疫苗免疫佐剂,将灭活的坦布苏病毒液和禽流感病毒(H9亚型)尿囊液分别与白油佐剂、卡波姆佐剂、铝胶佐剂、蜂胶佐剂制成抗原含量相同的TMUV-AIV二联灭活疫苗。应用阻断ELISA方法和血凝抑制方法测定免疫鸭血清中坦布苏病毒和禽流感病毒抗体,比较不同佐剂疫苗对试验鸭的免疫效力。结果表明,以白油作为佐剂的灭活疫苗组免疫效果最好,抗体产生快、抗体水平优于其他各组,免疫2周后60%(6/10)血清样品HI抗体可达1∶8以上,80%血清样品呈TMUV抗体阳性,是制备鸭TMUV-AIV(H9亚型)二联灭活疫苗的理想佐剂。 相似文献