The relationship between serum IGF-1, IGFBP-3 levels,bone mineral density and bone metabolic markers in postmenopausal patients with osteoporosis

AIM:To study the relationship between serum insulin-like growth factor- 1 (IGF-1), insulin-like growth factor binding protein-3 (IGFBP-3) levels, bone mineral density(BMD) and bone metabolic markers in postmenopausal women. METHODS:Serum IGF-1, IGFBP-3, osteocalcin(BGP), isomeric C-telopeptide of type I collagen (β-CTX), estradiol(E₂), calcitonin(CT), parathormone(PTH), calcium (Ca), and phosphorus(P) were measured in 90 postmenopausal osteoporosis patients and 70 healthy postmenopausal women. BMD of lumbar vertebra and left femoral neck were determined by dual energy X-ray absorptiometry.RESULTS:BMD of lumbar vertebra and left femoral neck decreased significantly (P<0.01), serum IGF-1, IGFBP-3, E₂, CT and BGP decreased significantly (P<0.01), serum β-CTX and PTH increased significantly in postmenopausal osteoporosis group (P<0.01). There were no significantly differences in serum Ca, P between two groups (P>0.05). BMD of lumbar vertebra and left femoral neck were positively correlated with serum IGF-1, IGFBP-3, E₂, CT and BGP, but negatively correlated with β CTX and PTH. There were no correlation with serum Ca, P and BMD.CONCLUSION:Serum IGF-1, IGFBP-3, E₂, CT, BGP, β-CTX and PTH level were correlated with BMD of lumbar vertebra and left femoral neck. These markers can be one of the valuable evidences for screening osteoporosis in postmenopausal women.     

依普黄酮对骨质疏松症笼养蛋鸡OPG和RANKL表达的影响

选用200只24周龄健康海兰褐蛋鸡,随机分为4组:对照组(基础日粮中含钙3.55％)、低钙组(日粮合钙1.27％)、试验Ⅰ组(依普黄酮8 mg/kg,低钙日粮)和试验Ⅱ组(依普黄酮20 mg/kg,低钙日粮),观察依普黄酮对骨质疏松症笼养蛋鸡血清及骨骼骨保护素(Osteo protegerin,OPG)/核因子-相受体活化因子配体(Receptor activatorof NF-κB ligand,RANKL)表达的影响.结果显示:与低钙组相比,30 d时,试验Ⅰ组和Ⅱ组血清OPG分别显著升高33.12％和60.19％(P＜0.05)；60 d时,试验Ⅰ组和Ⅱ组血清OPG分别显著升高35.89％和61.59％ (P＜0.05)；30 d时,试验Ⅰ组和Ⅱ组血清RANKL分别极显著降低63.85％和70.87％(P＜0.01)；60 d时,试验Ⅰ组和Ⅱ组血清RANKL分别极显著降低69.98％和72.85％ (P＜0.01).与低钙组相比,试验Ⅰ组和Ⅱ组骨组织OPG平均光密度分别显著(P＜0.05)和极显著(P＜0.01)升高,试验Ⅰ组和Ⅱ组蛋鸡骨组织RANKL平均光密度极显著低于低钙组蛋鸡(P＜o.01).试验Ⅰ组和Ⅱ组OPG/RANKL值高于低钙组.结果表明,低钙日粮中添加依普黄酮对蛋鸡骨质疏松症可起到一定改善作用,这可能与OPG上调,RANKL下调,影响RANKL信号通路,使其骨量增加,骨结构改善密切相关.     

Bone morphological analyses in Spontaneously Diabetic Torii (SDT) fatty rats

The Spontaneously Diabetic Torii (SDT) fatty rat, a model for obese type 2 diabetes, shows bone quantitative abnormalities, namely low bone mineral density (BMD). The objective of this study was to evaluate bone morphological changes, in particular identifying the bone qualitative abnormalities, in the SDT fatty rat. Male SDT fatty rats showed increases in total trabecular area and trabecular number and decreases in trabecular thickness in cancellous bones of the proximal tibia, indicating trabecular miniaturization. The SDT fatty rat is useful for investigation of pathophysiological changes in bone quality in diabetic osteoporosis.     

利用烟草表达一种新型鲑鱼降钙素的初步研究

利用烟草表达一种新型鲑鱼降钙素,以期为利用植物系统表达鲑鱼降钙素的研究提供理论参考。首先构建含外源基因“p il-m sCT”的植物双元表达载体p35S-2300-tw in T-DNA s∶p il-m sCT∶NO S-ter,然后通过农杆菌介导法转化烟草,获得卡那霉素抗性烟草128株,其中PCR阳性植株14株(阳性率10.94%);Sou thern b lotting分析表明外源基因已整合到烟草基因组中,拷贝数为2~8个;半定量RT-PCR结果显示,转基因烟草株间“p il-m sCT”转录水平存在较大差异。     

骨质疏松症治疗药物的研究进展

骨质疏松症是以骨量减少、骨的微观结构退化为特征,致使骨的脆性增加并易于发生骨折的一种全身性骨骼疾病,是当前严重危害人类特别是绝经妇女和老人的疾病之一.近年来出现了许多预防和治疗骨质疏松症的药物,极大地推动了骨质疏松症理论基础的完善和临床治疗研究.就该领域的研究进展进行了简要综述.     

绵羊实验性钼中毒研究Ⅶ.骨骼病变的X线观察

通过X线摄影检查,日服钼60、30mg/kg和日服钼15mg/kg另加服7g硫酸钠的羊,骨骼病变主要发生在四肢长管骨(以股骨为最多,肱骨次之),其实质为骨化性骨膜炎。根据病变波及的范围,有限局性和广泛性骨化性骨膜炎。前者常以骨赘的形式存在,后者多为周围性骨化性骨膜炎。骨骼病变出现的时间,最早为服药后68d,最晚为150d。经X线照片密度测定和骨骼的组织学检查证实,骨骼病变属骨质疏松。     

Musculoskeletal responses of 2-year-old Thoroughbred horses to early training. 8. Quantitative back-scattered electron scanning electron microscopy and confocal fluorescence microscopy of the epiphysis of the third metacarpal bone

AIM: To characterise and explain the increase in density evident by computerised tomography (CT) and radiography in companion studies as a response to training, in bone in the palmar and dorsal regions of the condyles of the third metacarpal bone (Mc3) of 2-year-old Thoroughbred horses.

METHODS: Compositional back-scattered electron (BSE) imaging in scanning electron microscopy (SEM) and confocal scanning laser microscopy (CSLM) were conducted on polymethyl methacrylate (PMMA)-embedded mediolateral slices of the right distal Mc3 from seven 2-year-old Thoroughbred horses trained on a racetrack and seven untrained horses kept at pasture. One left Mc3 from each group was studied in transverse section planes. This study focussed on regions of Mc3 found to differ in density between the trained and untrained horses in companion studies using CT and radiography.

RESULTS: The increase of bone density in the condyles of Mc3 in trained horses compared with untrained horses occurred, without prior osteoclastic resorption, via the deposition of new bone on pre-existing internal surfaces. Within prior marrow spaces of cancellous bone, there was also rapid formation of immature strands and fronds of bone which were more cellular and mineralised, and more lamellar bone tissue was deposited on these new scaffolding elements in the trained horses. Both resulted in increased bone volume fraction (BVF). The microscopic mineralisation density of the bulk of the new tissue was lower than in pre-existing bone, and CT and radiography underestimated the increase in BVF. The new tissue was thus probably less stiff at the microscopic scale than pre-existing bone, though its addition would stiffen the global structure.

CONCLUSIONS: In Mc3 of all the trained horses, there were obvious differences in microscopic structure compared with those from the untrained horses. Moderate, industry-standard levels of exercise used to prepare young horses for racing induced the formation of new bone in non-bone spaces in bone tissue, such that the bone organ should better withstand later increased levels of exercise.     

骨质疏松症的兽医临床研究进展

骨质疏松症是养殖业中危害畜禽健康的重要骨骼疾病之一,给养殖业带来了巨大的经济损失.从发病原因、临床症状、预防措施等几个方面对笼养蛋鸡、奶牛和羊的骨质疏松症进行了综述,以期为骨质疏松症的研究和兽医临床防治提供参考.     

中药复方制剂对大鼠和母猪骨质疏松症的防治效果试验

探讨中药复方对实验性大鼠骨质疏松症的预防和治疗作用,并在此基础上探索该中药对成年经产高龄母猪骨质疏松症的预防效果。对102只3月龄SPF级SD雌性大鼠用维甲酸造模并进行中药防治试验,造模结束和药物治疗结束后对大鼠进行骨密度检测、骨组织形态检测与血液生化指标(Ca、P、BALP和TRACP-5b)检测。同时,将35头6胎~8胎经产高龄母猪,4岁~5.5岁体重220kg^300kg长大二元杂母猪随机分为空白组、中药复方高、中、低(B1、B2、B3)3个剂量组,连续给药20d,检测母猪血液生化指标(Ca、P、TRACP-5b、CTX-Ⅰ、BALP和PINP)。结果表明,与模型组相比,中药组大鼠血清水平有显著降低,骨密度增加,骨微观结构得到改善;与空白组相比,中药中剂量组和高剂量组母猪血清BALP及PINP水平显著升高,TRACP-5b及CTX-Ⅰ水平显著降低。结果表明该中药复方制剂对大鼠和猪骨质疏松症的治疗有良好效果。