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采用高效液相色谱法测定不同干燥方法以及不同花梗长度下款冬花中款冬酮和绿原酸的含量。结果表明:采用传统阴干法,款冬花中款冬酮与绿原酸的含量均达到最高,不同干燥方法下款冬酮含量由高到低依次为60℃烘干70℃烘干40℃烘干晾晒50℃烘干30℃烘干;总体来看,绿原酸含量随着烘干温度升高而降低,款冬酮、绿原酸的含量随着花梗长度的增加而降低。综上所述,款冬花最佳干燥方法为传统阴干法。若需采用烘干法,应综合考虑温度对款冬酮、绿原酸含量的影响,最佳烘干温度为40℃;建议用款冬酮、绿原酸2个指标综合评价款冬花药材质量,一级款冬花花梗长度≤1 cm,二级款冬花花梗长度≤2 cm,统货花梗长度≤3 cm。 相似文献
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为研究励珂健喷剂(主要成分:杨树花提取物)对10日龄内仔猪腹泻和免疫力的影响,将18头PIC母猪随机分成3组,每组6头,分别为蒸馏水组、土霉素组和励珂健喷剂组,蒸馏水组仔猪1、3、5日龄时皮肤喷洒蒸馏水1 m L/头/d,土霉素组仔猪3、7日龄时分别肌肉注射20%长效土霉素注射液0.2 m L/头/d、0.5m L/头/d,励珂健喷剂组仔猪1、3、5日龄时皮肤喷洒励珂健喷剂1 m L/头/d。结果表明,励珂健喷剂可有效降低10日龄内仔猪的腹泻率和发病率,且励珂健喷剂组腹泻仔猪的治愈速度快于土霉素组和蒸馏水组。由此证明:励珂健喷剂对10日龄内仔猪腹泻有防治作用,并且还可以增强仔猪的免疫力。 相似文献
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四大药用菊花功能成分的比较研究 总被引:7,自引:1,他引:7
为了比较4种药用菊花的品质特征,采用比色法测定总黄酮含量,HPLC测定绿原酸含量,GC-MS分析挥发油的成分.结果表明,贡菊、滁菊、亳菊和杭菊中总黄酮的含量分别为5.608%、4.619%、4.372%和3.017%;绿原酸含量分别为0.345%、0.207%、0.337%和0.131%;挥发油总量分别为0.243%、0.524%、0.388%和0.212%;GC-MS分离鉴定出的化合物中贡菊有18种,滁菊有46种,亳菊有35种,杭菊有6种.可见贡菊的主要功能成分较其他3种菊花含量高,并且具有较好的香气品质,认为贡菊可能不仅更适合药用,同时适于作为饮料、功能和保健食品的原料. 相似文献
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四种杀菌剂对金银花白粉病的防治效果及对金银花品质的影响 总被引:1,自引:0,他引:1
采用随机区组试验比较四种杀菌剂对金银花白粉病的防治效果,用高效液相色谱法测定各处理样品的绿原酸含量,以筛选出符合金银花规范化种植需要的药剂。结果表明,施药后14天以15%三唑酮600倍液防治效果最好,达77.8%,其次是15%三唑酮1200倍、50%多菌灵500倍液处理,防治效果分别为75.7%和70.8%,极显著地高于3%中生菌素500和1000倍及2%农抗1201000倍液处理的防效。2%农抗120500倍液的防治效果达60.3%,与前三种处理差异不显著,显著好于后三种处理,但未达极显著水平。15%三唑酮1200倍防治处理的绿原酸含量最高,达1.683%,其次是50%多菌灵500倍处理,绿原酸含量达1.539%,符合《中华人民共和国药典》要求,可作为金银花规范化管理中白粉病防治优先采用的药剂。 相似文献
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Flos Chrysanthemi (the flower of Chrysanthemum morifolium Ramat.) is widely used in China as a food and traditional Chinese medicine for many diseases. Luteolin and apigenin are two main bioactive components in Flos Chrysanthemi, and chrysoeriol and diosmetin are two methylated metabolites of luteolin in vivo by cathechol-O-methyltransferase (COMT). However, there was lack of pharmacokinetic information of chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract (FCE). The present study aimed to develop an HPLC-UV method for simultaneous determination of rat plasma concentration of luteolin, apigenin, chrysoeriol and diosmetin and utilize it in pharmacokinetic study of the four compounds after orally giving FCE to rats. The method was successfully validated and applied to the pharmacokinetic study when oral administration of FCE to rats with or without co-giving a COMT inhibitor, entacapone. Chrysoeriol and diosmetin were detected in rat plasma after oral administration of FCE and their concentrations were significantly decreased after co-giving entacapone. Furthermore, AUC of luteolin was significantly increased by entacapone, while that of chrysoeriol was decreased by entacapone, which revealed COMT might play an important role in the disposition of luteolin in rats after dosing of FCE. In conclusion, a sensitive, accurate and reproducible HPLC-UV method for simultaneous determination of luteolin, apigenin, chrysoeriol and diosmetin in rat plasma were developed, pharmacokinetics of chrysoeriol and diosmetin combined with luteolin and apigenin were characterized after oral administration of FCE to rats, which gave us more information on pharmacokinetics and potential pharmacological effects of FCE in vivo. 相似文献