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91.
92.
This paper presents a model for freeway traffic flow simulation and prediction. The model uses cellular automation theory to model complex traffic behavior. The advantage of the cellular automata approach is that the roadway to be modeled is quantized into simple homogeneous cells, time is quanitzed into discrete steps, and physical quantities take on a finite set of values. Also, the state of the cells is updated at each discrete timestep by using a vehicle update algorithm that combines a few vehicle motion models, governed by a relatively small set of parameters. Then vehicles just move one or several cells at each discrete timestep according to the self-defined rule. This approach makes the computer operation feasible. At last, the paper puts forward a suppose that if the simulation system is equiped with the self-study system of NN (neural network) module according to the statistical data from the transducer fixed on the freeway, it can predict the traffic status ahead of 10 minutes. 相似文献
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Anagawa A Okazaki Y Murakami Y Tsubota K Ono M Matsumoto M Nakatsuji S Oishi Y 《Journal of toxicologic pathology》2009,22(3):205-208
A firm, tan, well-circumscribed mass that measured 25 × 30 × 35 mm was observed in the thoracic cavity of a 53-week-old male Crl:CD(SD) IGS rat. Histologically, the mass was encapsulated by fibrous tissue and contained fibrovascular septae. Tumor cells were compactly arranged, and most were oval to polygonal in shape with multivacuolated cytoplasm and a centrally located nucleus. In some parts of the tumor, marked cellular atypia and frequent mitoses were evident. Vacuoles in cytoplasm were positive for oil red O. The tumor cells were characterized ultrastructurally by abundant, round to oval mitochondria with transverse closely-packed cristae. Tumor cells were immunohistochemically positive for uncoupling protein 1 (UCP-1). Several thrombi and hemorrhagic or necrotic foci were also observed within the tumor mass. Vascular invasion of the tumor capsule was observed; however, invasion of surrounding tissues or metastases were not observed. Based on the pathology findings, this case was diagnosed as a malignant hibernoma. 相似文献
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猪胎儿成纤维细胞SOD1基因差异表达研究 总被引:1,自引:0,他引:1
在研究模式生物衰老机制的过程中,发现衰老受某些特定基因的调控。为研究细胞内铜锌超氧化物歧化酶(Cu/ZnSOD,SOD1) 在不同代数的猪胎儿成纤维细胞的差异表达,本试验收集正常传代生长的5,10,15,20,25,30,35,40,45,50,55,60,65,70代的猪胎儿成纤维细胞,分别提取细胞总RNA;采用RT-PCR技术,检测不同代数猪胎儿成纤维细胞SOD1的表达。结果表明猪SOD1在第5代猪胎儿成纤维细胞中表达水平最高,随着细胞代数的增加,表达量逐渐下降,到第70代表达量为最低。结果将为进一步揭示细胞衰老的分子机制提供有价值的参考,同时为丰富猪的分子生物学提供新素材。 相似文献
97.
研究脑炎原虫病兔外周免疫器官的形态学变化,采集20只病兔和12只对照兔的淋巴结、脾脏和圆小囊,通过临床检查,病理剖检,病理切片的HE染色、吉姆萨染色和免疫组织化学染色进行了比较观察。结果表明病兔淋巴结内的淋巴小结数量增多,生发中心明显,髓索增粗,副皮质区增宽。脾脏的脾小结增多,变大,具有明显的生发中心,边缘区明显增宽,动脉周围淋巴鞘明显变大,聚集大量淋巴细胞。圆小囊的淋巴小结增大,数量增多,生发中心明显,淋巴小结周围弥散大量淋巴细胞。通过α-ANAE和sABC染色,在淋巴结、脾脏和圆小囊中均可检出大量T细胞、巨噬细胞和IgG阳性细胞。据此认为患脑炎原虫病时,病兔的体液免疫和细胞免疫均增强,但以细胞免疫反应更强。 相似文献
98.
基因枪与肌肉注射猪繁殖与呼吸综合征病毒ORF5基因疫苗诱导小鼠体液及细胞免疫的比较研究 总被引:8,自引:2,他引:8
猪繁殖与呼吸综合征病毒ORF5基因疫苗(pcDNA—PRRSV—ORF5)以不同免疫途径(基因枪和肌肉注射)免疫BALB/c小鼠,以PBS和空载质粒pcDNA3.1(+)为对照,采用流式细胞仪(FACS)、淋巴细胞增殖试验(MTT法)及间接ELISA试验分别对小鼠外周血中CD4^+、CD8^+T淋巴细胞数、T淋巴细胞的转化功能及小鼠血清中特异性PRRSV血清抗体IgG动态变化进行了检测。结果表明,pcDNA—PRRSV-ORF5基因疫苗接种小鼠后外周血对ConA有明显的反应性,试验组与对照组比较差异极显著(P〈0.01)或显著(P〈0.05),CD4^+T淋巴细胞数在免疫后7d高于对照组(P〈0.01),CD8^+T淋巴细胞在免疫后28d高于对照组,不同途径基因疫苗接种小鼠后均诱导小鼠产生PRRSV特异性IgG。在诱导细胞免疫方面,基因枪和肌肉注射各组间无明显差异;在诱导体液免疫方面,基因枪法优于肌肉注射。研究表明制备的pcDNA—PRRSV—ORF5基因疫苗免疫小鼠能够诱导其机体产生良好的体液和细胞免疫应答,基因枪法较肌肉注射更能诱导体液免疫应答的产生。 相似文献
99.
Immunophenotyping of Leukocyte Subsets in Peripheral Blood and Palatine Tonsils of Prefattening Pigs
Terzić S Sver L Valpotić I Lojkić M Miletić Z Jemersić L Lacković G Kovsca-Janjatović A Orsolić N 《Veterinary research communications》2002,26(4):273-283
The quantitative and distribution patterns of porcine peripheral blood and tonsillar lymphoid/myeloid cell subsets were assessed in order to establish the immune status of farm pigs prior to their transfer to fattening units. Peripheral blood and tonsillar samples were taken from clinically healthy, nonvaccinated, 12-week-old pigs, either ex vivo or following euthanasia. Single-colour flow cytometry, using monoclonal antibodies (mAbs) reactive with the swine leukocyte cluster of differentiation (CD) antigens, gave the proportions of lymphoid (9.7% CD4+, 8.0% CD8+, 36.9% CD5a+, 20.3% CD16+, 6.9% CD21+, 86.3% CD45+, 41.8% CD45RA+, 48.3% CD45RC+), null cells (6.9%) and myeloid cells (23.7% CD11b+ and 5.4% SWC3a+) in peripheral blood. In situ identification and distribution of lymphoid cells in the tonsils (CD3a+, CD21+, CD45RA+, CD45RC+) was performed with anti-CD mAbs using the avidin–biotin complex method. Most CD3a+ cells were in the parafollicular areas, with many cells in the follicles. CD21+ cells were scattered throughout the parafollicular area, with only a few cells inside lymphoid follicles. CD45RA+ cells were mostly concentrated in the follicles but many positive cells were present in the parafollicular area. Many CD45RC+ cells were visible in the parafollicular area, a few positive cells were in the crypt epithelium, and single cells were inside the follicles. 相似文献
100.
为了构建Rep蛋白真核表达质粒,研究PCV2复制过程中的亚细胞分布。本研究借助PCR方法扩增PCV2的Rep基因,并将其亚克隆到真核表达载体pEGFP-C3中,并转染PK15细胞和Vero细胞。结果显示,重组的pEGFP—PCV2-Rep融合蛋白能够在PK15细胞和Vero细胞中表达。结论认为pEGFP—PCV2-Rep质粒转染PK15细胞和Vero细胞后48h,PCV2的Rep蛋白主要定位在PK15细胞和Vero细胞的细胞核。 相似文献