脑炎原虫病兔免疫器官的病理形态学观察

研究脑炎原虫病兔外周免疫器官的形态学变化,采集20只病兔和12只对照兔的淋巴结、脾脏和圆小囊,通过临床检查,病理剖检,病理切片的HE染色、吉姆萨染色和免疫组织化学染色进行了比较观察。结果表明病兔淋巴结内的淋巴小结数量增多,生发中心明显,髓索增粗,副皮质区增宽。脾脏的脾小结增多,变大,具有明显的生发中心,边缘区明显增宽,动脉周围淋巴鞘明显变大,聚集大量淋巴细胞。圆小囊的淋巴小结增大,数量增多,生发中心明显,淋巴小结周围弥散大量淋巴细胞。通过α-ANAE和sABC染色,在淋巴结、脾脏和圆小囊中均可检出大量T细胞、巨噬细胞和IgG阳性细胞。据此认为患脑炎原虫病时,病兔的体液免疫和细胞免疫均增强,但以细胞免疫反应更强。

Pathomorphologic Observation on Immune Organs of Rabbits with Encephalitozoon cuniculi

to research the morphological changes on peripheral immune organs of rabbits with encephalitozoon cuniculi,the lymph nodes,spleens and sacculus rotundus on 20 cases and 12 control rabbits were comparatively observed by clinic examination,the pathological anatomic inspection,the pathological sections stained with HE,Giemsa and immunohistochemistry.The results indicated that in rabbits with encephalitozoon cuinculi the lymph nodules in lymph node increased with an obvious germinal center,medullary cords become thicker and paracortex zone broadened.The splenic nodules in spleen increased and largened with an obvious germinal center.The marginal zone was widened obviously.Lots of lymphocytes gathered around the periarterial lymphatic sheath largened evidently.The lymph nodules in sacculus rotundus increased and largened with an obvious germinal center.A number of lymphocytes were diffused among lymph nodules.Staining with α-ANAE and sABC,A great number of T cells,macrophages and IgG-positive cells were detected in lymph nodes,spleens and sacculus rotundus.In view of the above,it was considered that in rabbits with encephalitozoon cuniculi the humoral immune and cellular immune were enhanced,but the cellular immune response was stronger.