产紫杉醇内生真菌O60B1的分离及鉴定

从曼地亚红豆杉(Taxus x media)树皮内表皮中分离得到1株产紫杉醇的内生真菌O60B1,并通过高效液相色谱法(HPLC)和质谱法(MS)分析其发酵产物,其紫杉醇产量为2.5～3.0 μg/g(紫杉醇/菌丝干重),并通过形态学特征和18 S rDNA序列分析,将其初步鉴定为毛霉属(Mucor sp.)真菌.     

Preliminary Studies on Antifungal Activity of Xanthium sibiricum and the Endophytic Fungi

[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization.     

苍耳提取液与植物内生真菌抗菌活性研究(英文)

[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization.     

苍耳提取液与植物内生真菌抗菌活性研究(英文)

[Objective] The study was aimed to provide theoretical foundation for developing a new fungicide with high activity,low toxicity and low residua.[Method] Based on the plant growth rate and inhibition zone methods,the antifungal activity of extracts of every organ of Xanthium sibiricum,the fermentation liquid of endophytic fungi MC4-23,and the extract of mycelium were tested with six kinds of pathogenic fungi and three kinds of pathogenic bacteria respectively.[Result] The extract of every organ of Xanthium sibiricum,the fermentation liquid of endophyic fungi MC4-23,and the extract of mycelium all had antifungal activities.The acetone extracts of Xanthium sibiricum had stronger antifungal activities than the ethanol extracts of Xanthium sibiricum.The extracts of leaf have the strongest antifungal activities against fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 47.8%,and the inhibition diameters of the three pathogenic bacteria were all above 0.5 centimeter.The fermentation liquid of endophytic fungi also had strong activities against the fungi and bacteria.The inhibition rates of the six pathogenic fungi were all above 68.1%.The extracts of root of Xanthium sibiricum and the mycelium of endophytic fungi were all had antifungal activities.[Conclusion] Xanthium sibiricum and endophyic fungi MC4-23 had great value of development and utilization.     

Isolation and Identification of an Endophytic Strain with Antibiosis Ability from Davidia involucrate Brail.

[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate Brail by using cylinder-plate method.Then,it was identified through physiological and biochemical tests,16S rDNA homology analysis as well as some gene-specific sequence analysis.[Result]B221 stain had antimicrobial activity against a variety of rice plant pathogens,and it was identified as Bacillus subtilis.[Conclusion]This study enriches the research on endophyte within D.involucrate Brail,application of Bacillus bio-control,and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens.     

链霉素抗性筛选吸水链霉菌高产农用抗生素菌株研究（英文）

[Objective] The research aimed to screen Streptomyces hygroscopicus strains with high production of agricultural antibiotics.[Method] A strain of S.hygroscopicus was screened from the soil of Hainan Island.After natural screening and consecutive ultraviolet induced mutation twice,S6-7 strain was obtained as the original strain then treated by UV irradiation and streptomycin resistance screening,and finally rescreened through shake-flask fermentation.[Result] 7 better strains were selected by primary screening from 62 single colonies which were picked out randomly.After 3 generations of consecutive cultivation on slant media and rescreening,5 strains presented obvious forward mutation.The forward mutation rate reached 8.06%,and the largest production increasing rate came up to 25.11%.[Conclusion] By combining streptomycin resistance screening and conventional ultraviolet induced mutation,both the antibiotic-producing capacity and forward mutation screening efficiency of the original strain were greatly enhanced.     

Isolation and Screening of Black Spot-Resistant Endophytic Fungi Strains from Rosa chinensis

[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.     

Optimization of Solid State Fermentation Conditions Using a Mixture of Bean Curd Residue and Marc with Bacillus natto

[Objective] The aim was to optimize the appropriate solid state fermentation(SSF)conditions.[Method] The optimization of solid state fermentation using a mixture substrate of bean curd residue and the marc with Bacillus natto was developed.[Result] The best fermentation condition optimized by the test of single factor and the orthogonal design respectively was mixing ratio of bean curd residue to marc 2∶1,substrate pH value 6,fermentation temperature 39 ℃,inoculum volume 10% and fermentation time 48 h.Under this optimized fermentation condition,the content of crude fiber in the substrate decreased from 107.8 mg/g before SSF to 56.2 mg/g after SSF,and the degeneration rate of crude fiber was 47.87%.[Conclusion] The bean curd residue in its palatability was enormously improved by SSF with Bacillus natto strain,which could be expected to be widely used as raw material of health foodstuff.     

Improvement of Ramoplanin Production by Mutation and Medium Optimization

The aim of this work was to develop a mutation and selection procedure to select the high-yield strain, and this strain could alleviate the inhibition in the biosynthesis of ramoplanin caused by L-leucine. In this experiment, the strain Actinoplanes sp. C6-78-14 that produced 384.8 mg of total productivity per liter was isolated. Its ramoplanin productivity was 4.13-fold higher than that of the parent strain A ctinoplanes sp. C2-9-45. The subculture experiments showed that the hereditary character of C6-78-14 was stable. Subsequent media studies with strain Actinoplanes sp. C6-78-14 showed that adding 0.001% FeSO4· 7H2O, 0.001% CoCl2· 6H2O and 0.001% CuSO4· 5H2O to fermentation medium respectively could enhance the ramoplanin production, and the production of each group was increased by 10%, 15% and 20% compared with the control group.     

高产谷胱甘肽酵母菌株的筛选及其抗乙硫氨酸诱变研究(英文)

[Objective] The aim of this study was to screen Saccharomyces for glutathione over-production. [Method] Ethionine-resistant mutants were obtained through UV mutagenesis and rational screening. [Result] A high GSH-producing strain HSJB1 was isolated from soil, and the biomass for this strain by flask shaking fermentation was 3.87 g/L while the GSH yield was 91.87 mg/L. According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. An ethionine-resistant mutant YBS77 was obtained through UV mutagenesis of the original strain HSJB1, and the biomass for this strain by flask shaking fermentation was 7.60 g dry cell weight/L while the GSH yield was 211.96 mg/L. [Conclusion] The biomass of the mutant obtained by breeding is increased by 96.38% than that of the original strain, and the GSH yield of the mutant obtained by breeding is increased by 130.72% than that from the original strain, which indicates that the breeding method is feasible.     

广西北海红树林土壤放线菌的分离与鉴定

[目的]鉴定广西北海红树林土壤放线菌的物种多样性.[方法]从广西北海红树林土壤中分离、筛选10株典型放线菌菌株,提取基因组DNA,进行16S rDNA PCR扩增与测序,并构建进化树.[结果]通过Blast比对,10株放线菌属于2个属,其中8株为链霉菌属(80%),2株为拟诺卡氏菌属(20%).[结论]广西北海红树林土壤蕴减着种类丰富的放线菌. Abstract： [Objective] The aim was to identify the species diversity of Actinomycetes from Mangrove forest in Beihai,Guangxi Province.[Method]10 strains of typical Actinomycetes were isolated from Mangrove forest soil,and the Actinomycetes genomic DNA was successful extracted.16S rDNA was amplified by PCR and sequenced by Sanger dideoxy sequencing method.[Rcsult]All the sequences were blasted in genbank,eight strains belonged to the genus of Streptomyces (80%),and two strains belonged to the genus of Nocardiopsis (20%).[Coacluslon]There are many different Actinomycetes species in Mangrove forest soil samples in Beihai,Guangxi Province.     

猪粪中高温纤维素分解菌的分离、筛选和鉴定

[目的]为研制高效生物降解菌剂奠定基础.[方法]在60℃条件下对猪粪中纤维素分解菌进行了分离研究,对分离到的菌株进行羧甲基纤维素酶活(CMCase)和滤纸酶活(FPase)测定,并进行16S rRNA基因分析.[结果]BC1和BC3号菌株CMCase、Fpase都较高,但相差不大,通过16S rDNA序列分析发现BC1和BC3号菌株分别与Pseudomonas sp.的相似性达到98%和99%.[结论]该研究为研制高效生物增温剂奠定了基础. Abstract： [Objective] The research aimed to lay the foundation for high-efficiency biological degradation microbial inoculums.[Method]under the 60℃ temperature the cellulolytic microbes in pig manure were isolated and determined the CMCase and Fpase,then proceeded the 16S rRNA gene analysis. [Result] The results showed that:BC1 and BC3 strains characterized higher carboxymethyl cellulose enzyme activity and higher filter paper activity,but their difference was small,then the 16S rDNA sequence of BC1 and BC3 strains were related to pseudomonas sp.(98% and 99% similarities,respectively).[Coaclusion]the experiment laid foundation for high-efficiency biological heating agent.     

基于蒙特卡洛多重积分的DNA动力学结构参数的计算

依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析. Abstract： Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.     

用最短路径传递共表达预测拟南芥基因功能

提出基因之间传递共表达可作为一个重要因素来连接同一代谢通路中的基因,而同一代谢通路中的功能相似的基因都是高表达相关的.因此可通过求代谢通路下的最短路径,南同在一条最短路径上的与未知基因高表达相关的已知基因来预测末知基因的功能.通过用最短路径算法分析拟南芥代谢通路下的共表达数据对未知基因的功能进行预测,证明了此方法可以预测出拟南芥代谢通路下未知基因的功能,并验证了通过在代谢通路下求最短路径来预测基因功能的方法具有一定的可行性和有效性. Abstract： The present paper predicted the function of unknow genes by analyzing the co-expression data of Arabidopsis thaliana from biological pathway based on the shortest-path algorithm.This paper proposed that transitive co-expression among genes can be used as an important attribute to link genes of the same biological pathway.The genes from the same biological pathway with similar functions are strongly correlated in expression.Moreover,the function of unknown genes can be predicted by the known genes where they are strongly correlated in expression lying on the same shortest-path from the biological pathway.Analyzing the Arabidopsis thaliana from the biological pathway,this study showed that this method can reliably reveal function of the unknown Arabidopsis thaliana genes and the approach of predicting gene function by transitiving coexpression in shortest-path is feasible and effective.     

催产素在广西本地水牛下丘脑-垂体-卵巢轴的免疫组化定位

[目的]研究催产素(OT)在广西本当水牛下丘脑、垂体及卵巢的分布上的联系,进而了解OT在下丘脑、垂体及卵巢3者之间分泌释放途径.[方法]采用免疫组织化学SuperPicTureTM二步法检测5头广西本地水牛的下丘脑、垂体和卵巢中催产素(OT)的分布情况.[结果]下丘脑中分泌OT的神经元主要分布在弓状核、视上核及室旁核,在腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑前核等核团也有一定数量的阳性神经元;在腺垂体发现OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部;卵巢中只在生殖上皮、卵泡颗粒细胞和黄体细胞发现有大量OT免疫阳性产物.[结论]首次发现OT存在于广西本地水牛下丘脑-垂体-卵巢轴的各个环节,并且下丘脑中各主要核团均有OT免疫阳性神经元的分布,尤其以弓状核、视上核、室旁核分布最多,为进一步研究OT的合成和作用机制提供形态学依据,并对广西本地水牛的繁殖育种及泌乳起到一定的参考指导作用. Abstract： To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuparPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo.The test results could provide morphology according to study the OT's synthesis and mechanism of action ,and could play reference and directions part in breeding Guangxi local buffalo.The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventrornedial nucleus,ventrolateralis nucleus,suprachiasrnaticus nucleus,dorsomediai nucleus,mamillary body,anterior hypothalamic nucleus and so on.The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence.In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein ceils.The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo.The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.     

干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响

[目的]研究干旱胁迫对甘蔗叶片水分和叶绿素荧光参数的影响,为甘蔗生产及评价研究提供依据.[方法]选取7个抗旱性不同的甘蔗品种,在苗期进行干旱胁迫,并测定胁迫条件下甘蔗叶片水分含量和叶绿素荧光参数的变化.[结果]甘蔗叶片水势和相对含水量与土壤相对含水量存在一定的内在联系,耐旱强的品种对土壤水分的利用率较高;相关分析和因子分析表明茁期干旱存活率、Fc/Fm、叶片水势和相对含水量可被用作抗旱性评价指标.[结论]水势表现为一个相对独立的影响因子,对甘蔗抗早性有支配作用,并验证了Fv/Fm作为甘蔗抗旱评价指标的可靠性. Abstract： [Objective] The aim was to explore the effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling,as well as to provide basis for the study on sugarcane production and evaluation.[Method]Seven different sugarcane varieties were studied at the seedling stage under drought stress,and the changes of leaf water and chlorophyll fluorescence parameters under stress conditions were detected. [Result] leaf water potential,leaf relative water content and soil relative water content showed a certain amount of internal relationship,the sugarcane varieties that had more tolerant to drought had higher utilization rate of soil water;the correlation analysis and factor analysis suggested that the survival rate at seedling stage under drought stress,Fv/Fm,leaf water potential and relative water content could be used as drought resistance evaluation indicators. [Conclusion] As a relatively independent influencing factor,water potential had dominating effect on drought resistance,and the reliability of Fv/Fm as drought resistance evaluation indicator had been verified.     

中草药提取液对黄瓜苗期杀根结线虫的活性研究

[目的]研究中草药提取液对黄瓜苗期杀根结线虫的活性.[方法]利用植物水培技术,结合生长条件易控制、根系生长过程易连续观察的特点,应用5种具有较强室内触杀根结线虫活性的植物提取液及其与阿维菌素的复配液,对黄瓜苗期水培根系杀线活性进行系统研究.[结果]胡黄连和石榴皮提取液可以在不影响水培黄瓜苗期植株生长的前提下表现出对根结线虫良好的活体植株根系寄生防治和杀灭活性,且达到了与阿维菌素相近的作用水平;而狗脊、木香和蛇床子提取液对黄瓜的生长表现出不同程度的抑制作用,其对根结线虫的防治效用和杀灭活性也较弱.[结论]触杀效果和杀线活性成分能否被植物吸收利用共同制约着具有杀线活性中草药提取液的开发应用. Abstract： Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the rootknot nematode parasitized on the cucumber seeding stage.It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin;while the extracts from Cibotium barornetz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth.     

不同土地利用方式下球囊霉素相关土壤蛋白与有机碳及土壤质地的关系

[目的]揭示土地利用方式对球囊霉素相关土壤蛋白(GRSP)的影响.[方法]测定了海南岛5种主要土地利用方式下GRSP、土壤有机碳(Soil oaganic carbon)与土壤质地(Soil texture)在0～10、10～20、20～50cm3个土壤层次中的分布情况.[结果]相对于保持较好的次生林,4种不同耕作土壤的GRSP与有机碳含量均有所下降.GRSP在海南岛5种主要土地利用方式土壤中的含量为0.53～4.80 mg/g,占有机碳的百分比为7.9%～23.4%,是碳库的重要组成部分.GRSP占有机碳的比例在不同土地利用方式下差异显著,在土层之间差异不显著,GRSP和有机碳含量在土壤剖面上垂直分布较明显.GRSP与有机碳、砂粒含量呈显著正相关,与粉粒和粘粒呈显著负相关.[结论]砂粒含量在很大程度上决定了CRSP的含量,壤土相对粘土更有利于GRSP的累积. Abstract： [Objective] The aim was to reveal the effects of different land use types on soil composition.[Method]GPRS,Soil organic carbon content and soil texture in 3 depths(0-10,10-20,20-50 cm)of 5 main kind of selected land use type were examined in Hainan. [Result] The results showed that GRSP and SOC content of four artificial land use types decreased compared with the natural sacondary foreSt land,the GRSP content of all samples ranged from 0.53-4.80 mg/g,accounting for 7.9%-23.4% of the SOC,which means that GRSP was one important component of SOC pool in soil.The ratio of GRSP to SOC was signiflcantly different among land use types but the depths.GRSP and SOC exhibited obvious vertical distribution pattern.GRSP was significantly positively related to SOC and sand content but negatively related to silt and clay content. [Conclusion] The sand content determined the GRSP content significantly and Joarn was better matrix for GRSP accumulation than clay.     

山区生态友好型土地利用对策研究——以重庆市黔江区为例

以黔江区为研究对象,通过对生态环境影响因子的分析,发现制约黔江区土地利用环境发展的因素主要有区域气候变化、土壤要素、水文环境以及水土流失和土地退化等.并任以上分析的基础上,进一步分析了土地利用变化对生态环境的影响和制约黔江生态环境及区域发展的因素,最后提出了适应山区土地利用与生态环境协凋的对策,对黔江社会经济发展和生态建设具有重要意义. Abstract： Accompanying the development of social economy,the land use model of mountainous area,typically eco-weak area,is changing gradually.Here the establishment of eco-friendly land use model in mountainous area,will pioneer the model of sustainable development in that area.Concerning Qianjiang District of Chongqing Municipality,huge change of land use model,mainly embodied in the unceasing increase of construction land and gradual decrease of agricultural use land,has taken place in recent years.To explore the eco-friendly land use model in mountainous area,Qianjiang District was chosen as the study object in the present study.Via analyzing the changes in land use model,we found that related eco-environment restrictive factors mainly regional,climatic change,soil texture,hydrological environment as well as soil erosion and land degradation,etc.And based on these results,we further analyzed the effect of land use change on eco-environment and the factors restricting the maintenance of eco-environment and regional development,finally put forward the counter measures for balancing land use and co-environment in mountainous area.The results will be important for the development of social economy and eco-system construction in Qianjiang District.     

水磷耦合对甘蔗经济性状的影响

[目的]研究不同供水量与施磷量对甘蔗经济性状的耦合效应.[方法]以广西甘蔗品种新台糖22号(ROC22)为材料,通过供水量设置3个水平,在甘蔗分蘖初期至伸长末期,每旬供水量分别为199.5m3/hm2(A1),400.5 m3/hm2(A2)、600.0 m3/hm2(A3);磷肥施用量设置4个水平,作为基肥分别施用P2O5 0 kg/hm2(B1)、120 kg/hm2(B2)、240 kg/hm2(B3)、360 kg/hm2(B4),来研究不同供水水平与磷肥水平组合处理对甘蔗产量和品质性状的影响.[结果]处理A2B2为较适合提高甘蔗经济性状的水磷配比处理.[结论]该研究结果为广西甘蔗生产中的水分、磷肥高效利用及甘蔗高产、高糖栽培提供理论依据. Abstract： [Objective] The aim was to study the coupling effect of water and phosphate on economic traits of sugarcane.[Method]Taking sugarcane variety ROC22 as tested material,coupling effects of different levels of water supply quantity and different levels of phosphorus fertilizer on the yield and quality of sugarcane were studied.Among them,water supply quantity had 3 levels,that was,the water supply quantity per 10 days from the early tillering stage of sugarcane to the end of elongation was 199.5 m3/hm2 (A1),400.5 m3/hm2 (A2) and 600.0 m3/hm2 (A3),respectively;Phosphorus fertilizer as basic fertilizer had 4 levels:P2O5 0 kg/hm2 (B1),120 kg/hm2 (B2),240 kg/hm2 (B3) and 360 kg/hm2(B4). [Result] Treatment A3B2 in water-fertilizer coupling was more suitable to improve economic traits of sugarcane. [Conclusion] The research results provide theoretical basis for the efficient utilization of water and phosphorus fertilizer in production of Guangxi sugarcane and the cultivation of high-yield and high-glucose sugarcane.