花期干旱胁迫对籼稻近等基因系水分和光合生理的影响

【Objective】An indica near-isogenic rice lines were treated with drought stress at flowering stage to study water and photosynthetic physiology changes of flag leaf. By analyzing the relationships among agronomic phenotype characters, water and photosynthetic physiology changes and fertility of rice, the authors try to accumulate data for drought tolerance evaluation of rice.【Method】The rice materials were suffered with drought stress begin from heading of main shoot for 15 days, water and photosynthetic physiology parameters were measured after treatment.【Result】The results showed that the indica near-isogenic rice represented various drought tolerance, and there was no correlation between drought tolerance and agronomic phenotype characters, neither with physiological activities of rice under well watered condition. However, under drought stress at flowering stage, the correlation coefficient between drought resistance indexes and changes of water content of flag leaf, water potential of flag leaf, stomatal conductance of flag leaf were 0.614**, 0.514** and 0.541**, respectively. This indicates that rice drought tolerance has a correlation with changes of water content, water potential and stomatal conductance. In addition, except the correlation coefficient between drought resistance indexes and changes of Fv/Fm of flag leaf (0.470*), there was no correlation between rice drought tolerance and photosynthetic physiology.【Conclusion】In summery, the changes of water physiology parameters could be used as indicators for screening rice with drought tolerance.     

水分胁迫对牡丹光合和荧光特性的影响(英文)

[Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [Method] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining the photosynthesis and fluorescence characteristics. [Result] With the aggravation of water stress,the net photosynthetic rate (Pn) and stomatal conductance (Gs) decreased,while the intercellular CO2 concentration (Ci) increased. Drought could decrease Pn,constrain gas exchange and change the daily photosynthesis. Fo of peony leaf increased and Fv/Fm decreased under water stress especially water logging,causing the inactivation of the PS II reaction center,and the chlorophyll fluorescence characters gradually recovered until afternoon. [Conclusion] The 75% soil relative water content (SRWC) is the best condition for growth of peony. Compared with drought,water logging is more unfit for the growth of peony. For the two varieties,Huhong assumed more tolerance to drought,accordingly more adaptability.     

Effects of Water Stress on Photosynthesis and Fluorescence Characteristics in Peony

[Objective] Study on the photosynthesis and fluorescence characteristics in peony under water stress. [Method] The two peony varieties Huhong and Luoyanghong were treated by different water stress for determining the photosynthesis and fluorescence characteristics. [Result] With the aggravation of water stress,the net photosynthetic rate (Pn) and stomatal conductance (Gs) decreased,while the intercellular CO2 concentration (Ci) increased. Drought could decrease Pn,constrain gas exchange and change the daily photosynthesis. Fo of peony leaf increased and Fv/Fm decreased under water stress especially water logging,causing the inactivation of the PS II reaction center,and the chlorophyll fluorescence characters gradually recovered until afternoon. [Conclusion] The 75% soil relative water content (SRWC) is the best condition for growth of peony. Compared with drought,water logging is more unfit for the growth of peony. For the two varieties,Huhong assumed more tolerance to drought,accordingly more adaptability.     

Screening of Drought Resistance Identification Indexes for Rice (Oryza sativa L.) Cultivars in Ningxia

The morphological characters, physiological characters, yield traits and yield per plant of total 49 rice cultivars from Ningxia were investigated under conditions of water stress and non-water stress so as to determine the relationship between each trait and yield per plant under water stress and the relationship between each relative character and drought resistance coefficient under water stress and non-water stress. The correlation, grey correlation, stepwise regression and path analyses showed that the til er number per plant, plant height, grain density, effective panicle number per plant and grain number per pan-icle, total 5 traits, were significantly correlated with the drought resistance of rice, and they could be used to identify the drought resistance of rice in Ningxia. In addition, the drought resistance of rice was graded qualitatively according to the subordinate function value of corresponding drought resistance coefficient. The results showed that among the 49 rice cultivars from Ningxia, 6 rice cultivars were highly drought resistant, and 9 rice cultivars were moderately drought resistant, suggesting that the evalua-tion method was feasible and effective.     

Effects of NaCl Stress on Chlorophyll Fluorescence Parameters in Cinnamomum japonicum var. chenii

[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.     

NaCl胁迫对普陀樟叶绿素荧光参数的影响(英文)

[Objective] Study on the changes of chlorophyll fluorescence parameters in Cinnamomum japonicum var. chenii under NaCl stress. [Method] The seedling growth increment,chlorophyll content and chlorophyll fluorescence parameters in leaves of 1-year old Cinnamomum japonicum var. chenii were investigated in field experiment. [Result] Under NaCl stress,seedling growth increment reduced and the chlorophyll content decreased to a stable value; changes of Fv/Fm and Fv/Fo showed identical increasing trend and double peak type. With the aggravation of salt stress,most variations were observed in Fo,correlations among chlorophyll fluorescence parameters presented "rise-drop" trend (in the treatment of 7 g/L NaCl). [Conclusion] Cinnamomum japonicum var. chenii is endowed with strong salt resistance and wide adaptability.     

Evaluation of Chlorophyll Content and Fluorescence Parameters as Indicators of Drought Tolerance in Barley

Drought is a major abiotic stress that severely affects food production worldwide. Agronomic and physiological traits associated with drought tolerance are suitable indicators for selection of drought tolerance genotypes to reduce the impact of water deficit on crop yield in breeding program. The objective of this study was to identify indicators related to drought tolerance through analysis of photosynthetic traits in barley （Hordeum vulgare L.）. These traits included chlorophyll content, initial fluorescence （Fo）, maximum primary yield of photochemistry of photosystem Ⅱ （Fv /Fo） and maximum quantum yield of photosystem Ⅱ （Fv/Fm）. Four genotypes （Tadmor, Arta, Morocco9-75 and WI2291） variable in drought tolerance were used to investigate the correlation between these traits and drought tolerance. The results reflected that all of these traits were affected negatively in the four genotypes at different levels of post-anthesis drought stress, but the decrease in drought tolerant genotypes was much less than that of drought sensitive genotypes. The results further revealed that the components of the photosynthetic apparatus could be damaged significantly in drought sensitive genotypes, while drought tolerant genotypes were relatively less affected. On the other hand, the values of chlorophyll content, Fo, Fv/Fo and Fv/Fm in drought tolerance genotypes were significantly higher than those in drought sensitive genotypes under drought stress. It was concluded that chlorophyll content, Fo, Fv/Fo and Fv/Fm could be considered as reliable indicators in screening barley germplasm for drought tolerance.     

Advances in Research on the Approaches of Improving Water Utilization Efficiency in Rice

Water shortage is increasingly an important factor limiting the sustainable development of global economy, posing a huge threat to social security and human existence. Water usage in agriculture accounts for about 70% of total water consumption in the world, and rice cultivation is in turn the largest water user, which accounts for about 50% of total water usage in agriculture. Therefore, it is quite important to improve water utilization efficiency to reduce water consumption in rice. Water stress causes severe inhibition of plant growth and development as well as yield reduction, however the extent of inhibition or reduction varies greatly with the growth stages, duration and severity of stress, and plant genotypes. In rice, drought resistance and water utilization efficiency might be improved by developing stress resistant cultivars and conducting proper agronomic practices. It is hence imperative to determine the suitable criteria in morphological and physiological traits for drought resistance and water utilization efficiency in conventional breeding of rice. At present, leaf rolling, leaf water potential and carbon isotope discrimination are commonly used criteria for the evaluation and identification of germplasm with high drought resistance or water utilization efficiency. With rapid development of molecular biology, marker-assisted selection has been used in rice breeding against water stress. In this review, therefore, the agronomic aspect of water saving techniques such as selection of suitable rice cultivars, planting pattern, mulching, deficit irrigation and alternative drying and moist irrigation are discussed and effective approaches are also recommended.     

Research Progress on Water Use Efficiency and Drought Resistance of Turfgrass

Improving turfgrass drought resistance and save-water features are very important to solve water deficiency in turfgrass production and management. This research did some surveys of systematic literature review about turfgrass drought tolerance morphological characteristics, physiological characteristics and water use efficiency, and provided the detailed information for enhancing turfgrass drought resistant and water use efficiency in the future.     

山东省蔬菜产地灌溉水重金属环境质量分析与评价

[目的]评价山东省蔬菜基地灌溉水重金属环境质量.[方法]对山东省主要蔬菜产地的灌溉水进行了重金属Hg、Cd、As、六价Cr、Pb、Cu和Zn含量的抽样调查分析,并采用单项质量指数与综合质量指数相结合的方法对重金属的环境质量状况进行了评价.[结果]寿光、莱阳、金乡和章丘4个蔬菜产地灌溉水中各重金属的平均含量均远低于《食用农产品产地环境质量评价标准》(HJ332-2006)规定的限值,未发现重金属含量超标现象;4地灌溉水重金属的单项质量指数均小于0.5,综合质量指数分别为0.3178、0.3204、0.2326和0.2607,灌溉水环境质量均为1级.[结论]4个蔬菜产地的灌溉水环境质量属于清洁水平,适宜作为无公害蔬菜的灌溉用水. Abstract： [Objective] The aim was to evaluate heavy metal environmental quality of irrigation water in vegetable farmlands of Shandong Province.[Method]Heavy metal contents including Hg,Cd,As,Cr (+6),Pb,Cu and Zn in irrigation water of main vegetable farmlands of Shandong Province were investigated by randomly sampling,and the environmental quality conditions of these heavy metals were evaluated by methods of single quality index and complex quality index. [Result] The results showed that the average contents of heavy metals in irrigation water of Shouguang,Laiyang,Jinxiang and Zhangqiu were all far lower than the limit values prescribed by "Farmland Environmental Quality Evaluation Standards for Edible Agricultural Products" (HJ332-2006),and no heavy metal was found beyond the limit value in every sample.The single quality indices of the 7 elements in the studied 4 places were all lower than 0.5.The comprehensive quality index of the seven elements was 0.317 8 in Shouguang,0.320 4 in Laiyang,0.232 6 in Jinxiang,and 0.260 7 in Zhangqiu.The environmental quality of irrigation water in the studied four places were all set at the first class. [Conclusion] The environmental quality of irrigation water in the 4 places belonged to clean level and were fit for the plantation of no pollution vegetables.     

水磷耦合对甘蔗经济性状的影响

[目的]研究不同供水量与施磷量对甘蔗经济性状的耦合效应.[方法]以广西甘蔗品种新台糖22号(ROC22)为材料,通过供水量设置3个水平,在甘蔗分蘖初期至伸长末期,每旬供水量分别为199.5m3/hm2(A1),400.5 m3/hm2(A2)、600.0 m3/hm2(A3);磷肥施用量设置4个水平,作为基肥分别施用P2O5 0 kg/hm2(B1)、120 kg/hm2(B2)、240 kg/hm2(B3)、360 kg/hm2(B4),来研究不同供水水平与磷肥水平组合处理对甘蔗产量和品质性状的影响.[结果]处理A2B2为较适合提高甘蔗经济性状的水磷配比处理.[结论]该研究结果为广西甘蔗生产中的水分、磷肥高效利用及甘蔗高产、高糖栽培提供理论依据. Abstract： [Objective] The aim was to study the coupling effect of water and phosphate on economic traits of sugarcane.[Method]Taking sugarcane variety ROC22 as tested material,coupling effects of different levels of water supply quantity and different levels of phosphorus fertilizer on the yield and quality of sugarcane were studied.Among them,water supply quantity had 3 levels,that was,the water supply quantity per 10 days from the early tillering stage of sugarcane to the end of elongation was 199.5 m3/hm2 (A1),400.5 m3/hm2 (A2) and 600.0 m3/hm2 (A3),respectively;Phosphorus fertilizer as basic fertilizer had 4 levels:P2O5 0 kg/hm2 (B1),120 kg/hm2 (B2),240 kg/hm2 (B3) and 360 kg/hm2(B4). [Result] Treatment A3B2 in water-fertilizer coupling was more suitable to improve economic traits of sugarcane. [Conclusion] The research results provide theoretical basis for the efficient utilization of water and phosphorus fertilizer in production of Guangxi sugarcane and the cultivation of high-yield and high-glucose sugarcane.     

北方主要造林树种耐旱机理及其分类模型的研究(Ⅰ)——苗木叶水势与土壤含水量的关系及分类

本文详细研究了我国北方部分主要造林树种苗木叶水势与土壤含水量的关系,结果表明:①苗木叶水势Ψ_w与土壤含水量SWC呈明显的“反J型”关系,应用双曲线方程y=a+b/x和指数方程y=ae~(b/x)拟合效果最优.②不同树种Ψ_w随SWC下降的变化差异很大,从而表明不同树种对土壤干旱胁迫的反应和适应不尽相同.依据Levitt和Kramer以及李吉跃等人对植物适应干旱方式的分类方法,应用系统聚类法将21个树种分为两大类4个亚类,即高水势延迟脱水树种(包括油松、樟子松、长白松、白皮松,华山松),亚高水势延迟脱水树种(包括二白杨、火炬树、华北落叶松),亚低水势忍耐脱水树种(包括文冠果、杜仲、沙枣、栾树、花椒、白榆、新疆杨.毛条、胡杨、红柳、侧柏),低水势忍耐脱水树种(包括山杏和山桃).相应于每一类为其建立了Fuzzy模式识别模型.这一模型可以在更为广阔的范围内判别其它树种的耐旱机理及其类型.     

底栖鱼类对水田上覆水中磷素动态的扰动效应

[目的]研究底栖鱼类泥鳅对水田上覆水中磷素动态的扰动效应,探讨生物扰动机制.[方法]基于模拟试验,使用离子色谱法和分光光度法,对比分析上覆水中磷素含量在有/无泥鳅活动时的差异.[结果]扰动组的TP、DTP和PP浓度在试验开始阶段与对照组无显著差异,在试验中、后期显著高于对照(P<0.05).扰动组要的PP/TP高于对照组,扰动组中TP浓度的增加主要是由于PP的增加,扰动组的DIP/DT在试验中、后期显著高于对照(P<0.05).[结论]底栖鱼类对水田上覆水中的磷素产生了扰动作用,增加了水稻生长可利用的的磷素养分. Abstract： [Objective] The research aimed to investigate the bioturbation effects of benthic fish Misgurnus anguillicaudatus on phosphorus dynamic in overlying water of paddy field,as well as to explore the bioturbation mechanism.[Method]Based on simulation experiment,the phosphorus contents in overlying water were analyzed comparatively with and without Misgurnus anguillicaudatus by the using of ion chromatography and spectrophotometry. [Result] The concentrations of total phosphorus (TP),dissolved total phosphorus(DTP)and particular phosphorus(PP) in bioturbation group had no significant differences with those in control group in initial stage of experiment,and became significantly higher than control group in middle and late stages of experiment(P<0.05).The PP/TP ratios in bioturbation group were bigger than those in control group,the increase of TP concentration in bioturbation group was mainly due to the increase of PP.The ratios of dissolved inorganic phosphorus(DIP) to DTP (DIP/DTP) were significantly bigger than those in control group in middle and late stages of experiment (P<0.05). [Conclusion] The benthic fish had bioturbation effects on phosphorus in overlying water of paddy field,which increased the available phosphorus for rice growth.     

吉林西部土壤微量营养元素有效量及其影响因素

通过对吉林西部3个工作区土样的微量营养元素全量、有效量以及土壤pH值和有机质含量的测定,分析了土壤微量营养元素有效量特征及其影响因素结果表明,吉林西部有效B基本不缺乏;有效Mn含量极丰富;有效Cu含量农安极丰富,明显高于其它2区,大安有效Cu含量样点间差异较大;土壤有效Zn基本不缺乏;有效Mo严重缺乏,且地区差异性较大;吉林西部有效Fe含量极丰富.土壤有效Mn、Cu和Fe含量受全量控制;B、Zn 元素有效量受土壤pH值的制约;土壤有机质则与有效Mn和Fe呈极显著正相关. Abstract： Based on the assay of the total content and available content of the trace nutrient elements and the soil pH and organic matter of the soil samples,the characteristic of the available content of the trace nutrient elements and their affecting factors are studied.The results show that the available B in western Jilin is in a middle level,the content in Nong'an is higher than that in the others;the available Mn is extremely abundant;the available Cu in Nong'an is obviously higher than other areas,and the content in Da'an differs greatly;the available Zn is in a middle level;the severe shortage of available Mo differs between different areas;and the available Fe is extremely abundant.The available Mn,Cu and Fe have significantly positive correlation with their total content;the available B has significantly positive correlation with pH,and Zn has obviously negative correlation with pH;the available Mn and Fe had significantly positive correlation with soil organic matter.     

干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响

[目的]研究干旱胁迫对甘蔗叶片水分和叶绿素荧光参数的影响,为甘蔗生产及评价研究提供依据。[方法]选取7个抗旱性不同的甘蔗品种,在苗期进行干旱胁迫,并测定胁迫条件下甘蔗叶片水分含量和叶绿素荧光参数的变化。[结果]甘蔗叶片水势和相对含水量与土壤相对含水量存在一定的内在联系,耐旱强的品种对土壤水分的利用率较高;相关分析和因子分析表明苗期干旱存活率、Fv/Fm、叶片水势和相对含水量可被用作抗旱性评价指标。[结论]水势表现为一个相对独立的影响因子,对甘蔗抗旱性有支配作用,并验证了Fv/Fm作为甘蔗抗旱评价指标的可靠性。     

吐丝期干旱胁迫对玉米生长及产量的影响

为促进玉米抗旱栽培,以先玉335为材料,采用大田小区对比试验,开展吐丝期干旱胁迫对玉米生长及产量影响的研究。通过对土壤含水量、玉米叶片形态和生理、果穗性状及产量等指标的测定,分析干旱胁迫对玉米生长及产量的影响。结果表明:土层0~20cm、20~40cm和40~60cm,不同处理的土壤含水量显著差异(P0.05),无干旱胁迫(T2)干旱胁迫5d(T3)干旱胁迫10d(T4)干旱胁迫15d(T5)自然生长(T1)。叶片卷曲度T1T5T4T3T2。叶片保绿性、相对含水量、SPAD值均为T2T3T4T5T1。T2叶片相对含水量分别比T1、T3、T4、T5高55.38%、5.31%、24.17%和37.91%,T2的SPAD值分别比T1、T3、T4、T5高38.70%、9.45%、18.97%和27.10%。干旱胁迫对玉米性状及产量具有影响,干旱导致玉米穗长缩短,秃尖变长,穗粗、行粒数减少,产量下降(P0.05),T2产量分别比T1、T3、T4和T5高50.25%、8.94%、15.06%和28.12%。     

水分胁迫对洛麦9133幼苗叶片生理特性的影响

盆栽试验研究了水分胁迫条件下洛麦9133幼苗叶片生理特性。结果表明:随土壤水分的减少,幼苗叶水势、叶片相对含水率、叶绿素a、叶绿素b和叶绿素总量均呈降低趋势;叶片水分饱和亏、可溶性糖含量、脯氨酸含量、质膜透性、MDA含量以及叶片SOD、POD活性则均呈增加趋势。说明,在干旱胁迫下,洛麦9133幼苗叶片通过改变内部的生理特性以提高自身的抗旱性。     

基于蒙特卡洛多重积分的DNA动力学结构参数的计算

依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析. Abstract： Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.