The design of aided software for osmotic stress responding genes mining in plant genome

A software and algorithm which based on random sequence model uses osmotic stress responding cis elements from existing information sources of biology was designed.It can infer the genic downstream function of Arabidopsis thaliana through analyzing its promoter region,and can offer effective aided analysis to mine osmotic stress responding genes in Arabidopsis thaliana genome.The practical application proves that this software can aid to analyze vast genic data and offer important data evidence.     

用最短路径传递共表达预测拟南芥基因功能

提出基因之间传递共表达可作为一个重要因素来连接同一代谢通路中的基因,而同一代谢通路中的功能相似的基因都是高表达相关的。因此可通过求代谢通路下的最短路径,由同在一条最短路径上的与未知基因高表达相关的已知基因来预测未知基因的功能。通过用最短路径算法分析拟南芥代谢通路下的共表达数据对未知基因的功能进行预测,证明了此方法可以预测出拟南芥代谢通路下未知基因的功能,并验证了通过在代谢通路下求最短路径来预测基因功能的方法具有一定的可行性和有效性。     

Cloning and Expression Level Analysis of Two BnaANT Candidate Genes in Brassica napus

AINTEGUMENTA （ANT） gene has been proved to have a novel function on controlling the organ size and the seed weight in Arabidopsis thaliana, the research on its orthologous gene in Brassica napus will be of potential interest to elucidate the molecular mechanism of rapeseed development and increase the rapeseed output. A SMART cDNA library of the flower bud from B. napus cultivar Zhongshuang 9 was constructed, and the full-length cDNAs of two homologous BnaANT candidate genes were cloned by PCR in B. napus, namely BnaX.ANT.a （GenBank accession no. DQ211969） and BnaX.ANT.b （accession no. DQ211970）. They shared 87.1 and 83.4% amino acid identity with ANT of A. thaliana, respectively. There is 87.4% amino acid identity between BnaX.ANT.a and BnaX.ANT.b. By quantitative real-time PCR, the obviously differential expression levels of the two BnaANT candidate genes were detected in the 28 DAF seeds of the big and small grains B. napus lines, the expression abundance of BnaANT in the 28 DAF seed of 9311 was over three times compared to that of 9260, which indicates that these two BnaANT genes are also likely related to the floral organ size and the seed weight in B. napus.     

雄性育性基因RNA干扰载体的构建与鉴定(英文)

cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harboring MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines.     

Isolation and Characterization of a Cinnamoyl CoA Reductase Gene（CCR） in Pear（Pyrus pyrifolia）

Pear is a popular and commercially important fresh fruit, and its texture is related to the presence of sclereid formatted by parenchyma cell with lignification in vascular plants. Previous studies have demonstrated that content of lignin may be regulated by cinnamoyl CoA reductase（CCR） in various plants. However, the function of CCR in pears remains very limited. In the present study, we isolated a cDNA encoding CCR（PpCCR, GenBank accession No. KF999958） and its promoter（proPpCCR） from Whangkeumbae pear to investigate the function of CCR in lignin biosynthesis. PpCCR-GFP expressed in rice mesophyll protoplast demonstrated that PpCCR-GFP was localized in the cytoplasm, indicating that CCR may function in cytoplasm without localization signals. In transgenic plants carrying PpCCR, we observed higher lignin content compared with that in wild type plants, further suggesting that PpCCR can affect the lignin contents through regulating lignin biosynthesis in Arabidopsis thaliana. More studies in other plants are needed to confirm our conclusion.     

The Prediction of Rice Gene by Fgenesh

This study has been carried out to give some scientific reasons for genome annotation, shorten the annotating time, and improve the results of gene prediction. Taking the sequence of the 6th chromosome, which has more length sequences than others, of Oryza sativa L. ssp. japonica cv. Nipponbare as analysis data in this research, the gene prediction of monocots module, rice, has been done by using Fgenesh ver. 2.0, and the predicting results have been explored particularly by bioinformatics methods. Results showed that the number of predicted genes for this chromosome was very close to the number of TIGR annotated genes. The majority of the predicted genes were multi-exon genes which had a percentage of 77.52. Length range was very big in the predicted genes. According to the significant match number, multi-exon genes can be predicted more veracity than single exon genes and the support can be reached up to 100% by TIGR annotation and up to 78% by cDNA. From the angle of predicted exons location of multi-exon genes, the internal exons and last exons had a high support of cDNA. The length of internal exons was relatively short in high （〉95% length, 〉78% similarity） cDNA and/or TIGR annotation support multi-exon genes, but the first exons and last exons were on the reverse. The majority of single exon genes which had more than 95% in length, and 78% in similarity support by cDNA and/or TIGR annotation was relatively short in length. From the angle of exon number, the majority of the multi-exon genes of high （〉 95% length, 〉 78% similarity） cDNA and/or TIGR annotation support had no more than 5 exon number. It was concluded that the rice gene prediction by Fgenesh was very good but needed modification manually to some extent according to cDNA support after aligning the predicting sequence of genes with cDNA database of rice.     

牛体外发育胚胎特定阶段差异表达基因的研究

[目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异.[方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比.[结果]该序列与牛核糖体蛋白131基因(ribosomal protein 131,RPL3])具有99%的同源性.采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍.[结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据. Abstract： The cattle different stage embryos obtained from in vitro was studied using the technology of single preimplantation embryo mRNA different display:single 8-cell and blastocyst stage embryos were studied using technology of mRNA different display and one different fragment was found.The result suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31.Then to detect the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos by real-time quantitative PCR,the result showed the relative amount of 8 cells was 3.2 times of blastocyst's.     

质粒pUC19-CM-D的构建及应用

[目的]用克降载体pUC19构建乳杆菌自杀质粒及乳杆菌基因缺失工程菌.[方法]以鼠李糖乳杆菌为研究对象,在质粒pUC19的多克隆位点插入氯霉素抗性基因构建pUC19-CCM载体;在pUC19-CM载体氯霉抗性基因的两侧均添加1个用于同源重组的同源臂,再构建成自杀质粒pUC19-CM-D.将自杀质粒pUC19-CM-D.将自杀质粒pUC19-CM-D转化乳杆菌进行抗生筛选即可得到目标基因被抗性基因替换的突变株.[结果]通过改造pUC19质粒,获得了可应用于构建鼠李糖乳杆菌D-乳酸脱氢酶基因缺陷型菌株的自杀质粒pUC19-CM-D.运用抗性基因替换方法来构建基因缺陷型菌株,在一定程度上避免了质粒整合带来的问题,同时提供了一个筛选标记,能够通过一次筛选即可得到缺陷型菌株.这种自杀质粒的改造和基因缺陷型菌株的构建方法具有通用性,可以应用于其他的载体和菌株.[结论]pUC19-CM-D质粒的构建及应用为乳杆菌基因缺失工程菌的构建提供了1个快速有效的手段,也为乳杆菌基因功能研究奠定了基础. Abstract： [Objective] The aims were to construct a new suicide plasmid of Lactobacillus and gene deletion engineering bacteria of Lactobacillus with pUC19 vector,[Methods]pUC19-CM was constructed by inserting a chloramphenicol resistant gene into the multi-cloning site of pUC19,and then two homologous fragments were cloned into each side of the pUC19-CM to construct suicide plasmid pUC19-CM-D.[Results]A replacement mutant strain,whose target gene was replaced by resistant gene,could be obtained by transforming the suicide plasmid pUC19-CMD into Lactobacillus for resistance screening. [Conclusion] The construction and application of pUC19-CM-D provided a fast and efficient means of construction of gene deletion engineering bacteria of Lactobacillus,and laid a foundation for study of gene function of Lactobacillus.     

基于蒙特卡洛多重积分的DNA动力学结构参数的计算

依据最新NDB数据库中蛋白质-DNA复合物晶体结构数据,基于修正的DNA结构统计力学模型,利用蒙特卡洛多重积分计算DNA动力学结构的有关参数,并对计算得到的结果进行时间复杂度和精确度分析. Abstract： Based on protein-DNA complex crystal structural data in up-to-date Nucleic Acid Database,the related parameters of DNA Kinetic Structure were investigated by Monte-Carlo Multiple Integrals on the base of modified DNA structure statistical mechanical model,and time complexity and precision were analyzed on the calculated results.     

催产素在广西本地水牛下丘脑-垂体-卵巢轴的免疫组化定位

[目的]研究催产素(OT)在广西本当水牛下丘脑、垂体及卵巢的分布上的联系,进而了解OT在下丘脑、垂体及卵巢3者之间分泌释放途径.[方法]采用免疫组织化学SuperPicTureTM二步法检测5头广西本地水牛的下丘脑、垂体和卵巢中催产素(OT)的分布情况.[结果]下丘脑中分泌OT的神经元主要分布在弓状核、视上核及室旁核,在腹内侧核、腹外侧核、交叉上核、背内侧核、乳头体、下丘脑前核等核团也有一定数量的阳性神经元;在腺垂体发现OT免疫反应阳性产物,自垂体柄和正中隆起的一侧可见到平行排列的OT阳性神经纤维断续地延伸至神经部;卵巢中只在生殖上皮、卵泡颗粒细胞和黄体细胞发现有大量OT免疫阳性产物.[结论]首次发现OT存在于广西本地水牛下丘脑-垂体-卵巢轴的各个环节,并且下丘脑中各主要核团均有OT免疫阳性神经元的分布,尤其以弓状核、视上核、室旁核分布最多,为进一步研究OT的合成和作用机制提供形态学依据,并对广西本地水牛的繁殖育种及泌乳起到一定的参考指导作用. Abstract： To study the association of oxytocin (OT)'s distribution in hypothalamatic,pituitary and ovary,and understand how the OT secrete releasing in hypothalamus,pituitary and ovaries,the paraffin section immunohistochemistry SuparPicTureTM two step method was used to detect the distribution of OT in hypothalamatic-pituitary-ovary axis of five femal Guangxi local buffalo.The test results could provide morphology according to study the OT's synthesis and mechanism of action ,and could play reference and directions part in breeding Guangxi local buffalo.The test results display:oxytocin immuno reactive (OT-IR) neuronsw eremainly distributed arcuate nucleus,supraoptic nucleus and paraventricular nucleus,and OT-IR neurons was also found in ventrornedial nucleus,ventrolateralis nucleus,suprachiasrnaticus nucleus,dorsomediai nucleus,mamillary body,anterior hypothalamic nucleus and so on.The OT immunoactive production was found in pituitary and few OT-IR nerve fibers extended to post pituitary from hypophyseal stalk and medium eminence.In ovaries,OT immunoactive productions were only distributed in germinal epithelium cells,granulosa cells and lutein ceils.The OT was first discovered in singulorum link of hypothalamatic-pituitary-ovary axis of Guangxi local buffalo.The OT immunoactive neurons were first discovered in every main nucleus of Guangxi local buffalo hypothalamus,especially distributed in arcuate nucleus,supraoptic nucleus and paraventricular nucleus.     

干旱胁迫对苗期甘蔗叶片水分和叶绿素荧光参数的影响

[目的]研究干旱胁迫对甘蔗叶片水分和叶绿素荧光参数的影响,为甘蔗生产及评价研究提供依据.[方法]选取7个抗旱性不同的甘蔗品种,在苗期进行干旱胁迫,并测定胁迫条件下甘蔗叶片水分含量和叶绿素荧光参数的变化.[结果]甘蔗叶片水势和相对含水量与土壤相对含水量存在一定的内在联系,耐旱强的品种对土壤水分的利用率较高;相关分析和因子分析表明茁期干旱存活率、Fc/Fm、叶片水势和相对含水量可被用作抗旱性评价指标.[结论]水势表现为一个相对独立的影响因子,对甘蔗抗早性有支配作用,并验证了Fv/Fm作为甘蔗抗旱评价指标的可靠性. Abstract： [Objective] The aim was to explore the effects of water stress on leaf water and chlorophyll fluorescence parameters of sugarcane seedling,as well as to provide basis for the study on sugarcane production and evaluation.[Method]Seven different sugarcane varieties were studied at the seedling stage under drought stress,and the changes of leaf water and chlorophyll fluorescence parameters under stress conditions were detected. [Result] leaf water potential,leaf relative water content and soil relative water content showed a certain amount of internal relationship,the sugarcane varieties that had more tolerant to drought had higher utilization rate of soil water;the correlation analysis and factor analysis suggested that the survival rate at seedling stage under drought stress,Fv/Fm,leaf water potential and relative water content could be used as drought resistance evaluation indicators. [Conclusion] As a relatively independent influencing factor,water potential had dominating effect on drought resistance,and the reliability of Fv/Fm as drought resistance evaluation indicator had been verified.     

中草药提取液对黄瓜苗期杀根结线虫的活性研究

[目的]研究中草药提取液对黄瓜苗期杀根结线虫的活性.[方法]利用植物水培技术,结合生长条件易控制、根系生长过程易连续观察的特点,应用5种具有较强室内触杀根结线虫活性的植物提取液及其与阿维菌素的复配液,对黄瓜苗期水培根系杀线活性进行系统研究.[结果]胡黄连和石榴皮提取液可以在不影响水培黄瓜苗期植株生长的前提下表现出对根结线虫良好的活体植株根系寄生防治和杀灭活性,且达到了与阿维菌素相近的作用水平;而狗脊、木香和蛇床子提取液对黄瓜的生长表现出不同程度的抑制作用,其对根结线虫的防治效用和杀灭活性也较弱.[结论]触杀效果和杀线活性成分能否被植物吸收利用共同制约着具有杀线活性中草药提取液的开发应用. Abstract： Present mature plants hydroponic technology was used,combined with some excellent characteristics,such as growth conditions was easy to control and process of root growth was easy to continuously observe,the nematicidal activity of 5 kinds of Chinese herbs extracts and the compound solution of Avermectin,with strong contact toxicity effect indoor,was systematically studied and investigated the affection on the rootknot nematode parasitized on the cucumber seeding stage.It is found that under the premise of no influence on root growth of cucumber,extracts from Picrorhiza scrophulariiflora and Punica granatum showed strong prevention and nematicidal activity,and had the similar efficacy of Avermectin;while the extracts from Cibotium barornetz,Aucklandia lappa Decne and Fructus cnidii showed low nematicidal activity and various degrees inhibition effect on plant growth.     

不同土地利用方式下球囊霉素相关土壤蛋白与有机碳及土壤质地的关系

[目的]揭示土地利用方式对球囊霉素相关土壤蛋白(GRSP)的影响.[方法]测定了海南岛5种主要土地利用方式下GRSP、土壤有机碳(Soil oaganic carbon)与土壤质地(Soil texture)在0～10、10～20、20～50cm3个土壤层次中的分布情况.[结果]相对于保持较好的次生林,4种不同耕作土壤的GRSP与有机碳含量均有所下降.GRSP在海南岛5种主要土地利用方式土壤中的含量为0.53～4.80 mg/g,占有机碳的百分比为7.9%～23.4%,是碳库的重要组成部分.GRSP占有机碳的比例在不同土地利用方式下差异显著,在土层之间差异不显著,GRSP和有机碳含量在土壤剖面上垂直分布较明显.GRSP与有机碳、砂粒含量呈显著正相关,与粉粒和粘粒呈显著负相关.[结论]砂粒含量在很大程度上决定了CRSP的含量,壤土相对粘土更有利于GRSP的累积. Abstract： [Objective] The aim was to reveal the effects of different land use types on soil composition.[Method]GPRS,Soil organic carbon content and soil texture in 3 depths(0-10,10-20,20-50 cm)of 5 main kind of selected land use type were examined in Hainan. [Result] The results showed that GRSP and SOC content of four artificial land use types decreased compared with the natural sacondary foreSt land,the GRSP content of all samples ranged from 0.53-4.80 mg/g,accounting for 7.9%-23.4% of the SOC,which means that GRSP was one important component of SOC pool in soil.The ratio of GRSP to SOC was signiflcantly different among land use types but the depths.GRSP and SOC exhibited obvious vertical distribution pattern.GRSP was significantly positively related to SOC and sand content but negatively related to silt and clay content. [Conclusion] The sand content determined the GRSP content significantly and Joarn was better matrix for GRSP accumulation than clay.     

山区生态友好型土地利用对策研究——以重庆市黔江区为例

以黔江区为研究对象,通过对生态环境影响因子的分析,发现制约黔江区土地利用环境发展的因素主要有区域气候变化、土壤要素、水文环境以及水土流失和土地退化等.并任以上分析的基础上,进一步分析了土地利用变化对生态环境的影响和制约黔江生态环境及区域发展的因素,最后提出了适应山区土地利用与生态环境协凋的对策,对黔江社会经济发展和生态建设具有重要意义. Abstract： Accompanying the development of social economy,the land use model of mountainous area,typically eco-weak area,is changing gradually.Here the establishment of eco-friendly land use model in mountainous area,will pioneer the model of sustainable development in that area.Concerning Qianjiang District of Chongqing Municipality,huge change of land use model,mainly embodied in the unceasing increase of construction land and gradual decrease of agricultural use land,has taken place in recent years.To explore the eco-friendly land use model in mountainous area,Qianjiang District was chosen as the study object in the present study.Via analyzing the changes in land use model,we found that related eco-environment restrictive factors mainly regional,climatic change,soil texture,hydrological environment as well as soil erosion and land degradation,etc.And based on these results,we further analyzed the effect of land use change on eco-environment and the factors restricting the maintenance of eco-environment and regional development,finally put forward the counter measures for balancing land use and co-environment in mountainous area.The results will be important for the development of social economy and eco-system construction in Qianjiang District.     

水磷耦合对甘蔗经济性状的影响

[目的]研究不同供水量与施磷量对甘蔗经济性状的耦合效应.[方法]以广西甘蔗品种新台糖22号(ROC22)为材料,通过供水量设置3个水平,在甘蔗分蘖初期至伸长末期,每旬供水量分别为199.5m3/hm2(A1),400.5 m3/hm2(A2)、600.0 m3/hm2(A3);磷肥施用量设置4个水平,作为基肥分别施用P2O5 0 kg/hm2(B1)、120 kg/hm2(B2)、240 kg/hm2(B3)、360 kg/hm2(B4),来研究不同供水水平与磷肥水平组合处理对甘蔗产量和品质性状的影响.[结果]处理A2B2为较适合提高甘蔗经济性状的水磷配比处理.[结论]该研究结果为广西甘蔗生产中的水分、磷肥高效利用及甘蔗高产、高糖栽培提供理论依据. Abstract： [Objective] The aim was to study the coupling effect of water and phosphate on economic traits of sugarcane.[Method]Taking sugarcane variety ROC22 as tested material,coupling effects of different levels of water supply quantity and different levels of phosphorus fertilizer on the yield and quality of sugarcane were studied.Among them,water supply quantity had 3 levels,that was,the water supply quantity per 10 days from the early tillering stage of sugarcane to the end of elongation was 199.5 m3/hm2 (A1),400.5 m3/hm2 (A2) and 600.0 m3/hm2 (A3),respectively;Phosphorus fertilizer as basic fertilizer had 4 levels:P2O5 0 kg/hm2 (B1),120 kg/hm2 (B2),240 kg/hm2 (B3) and 360 kg/hm2(B4). [Result] Treatment A3B2 in water-fertilizer coupling was more suitable to improve economic traits of sugarcane. [Conclusion] The research results provide theoretical basis for the efficient utilization of water and phosphorus fertilizer in production of Guangxi sugarcane and the cultivation of high-yield and high-glucose sugarcane.     

三峡库区消落区生态安全预警系统研究

简要介绍了三峡库区消落区的面积、范围以及可能出现的生态环境问题,初步构建了消落区生态安全预警框架,包含生态安全动态监测、生态安全评价、生态安全警情预报和生念安全决策管理4个子系统.并针对消落区可能出现的生念环境问题初步建立了生态安全综合评价指标体系,包含生态环境污染、土地利用与土地覆被变化、地质灾害和流行病病情、疫情4个预警评价.同时选取29个评价指标,分为基础信息因子数据、联动影响因了数据和致灾诱发因子数据,其中致灾诱发因子数据需要实时搜集乃至实时监控. Abstract： The area.the scope as well as some ecological environment questions in Three Gorges Reservoir was briefly introduced.Then its early warning-system frame was preliminarily constructed,which includes ecological security dynamic monitoring,ecological security appraisal,ecological security forecast and ecological security decision-making management.The synthetic evaluation indicator system of the ecological security quality were initially established,which includes ecological environment pollution,land use and land cover change,geological hazard and epidemic outbreaks.At the same time,29 evaluating indicators were selected,divides into the basic factors,response factors and inducing factors,which need to be Real-time monitored.