牛体外发育胚胎特定阶段差异表达基因的研究 |
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引用本文: | 栗雪冰,仓明.牛体外发育胚胎特定阶段差异表达基因的研究[J].农业科学与技术,2010(5). |
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作者姓名: | 栗雪冰 仓明 |
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作者单位: | 内蒙古大学哺乳动物生殖生物学和生物技术教育部重点实验室,内蒙古呼和浩特,010021 |
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基金项目: | 国家"863"项目(2008AA101007).Supported by National "863" Project |
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摘 要: | 目的]研究不同发育时期牛体外受精胚胎在基因表达模式上的差异.方法]利用单个胚胎mRNA差异显示技术,对单个8细胞期胚胎与囊胚进行mRNA差异显示,获得1条特异表达条带,对其进行克隆、测序,并与GenBank进行对比.结果]该序列与牛核糖体蛋白131基因(ribosomal protein 131,RPL3])具有99%的同源性.采用实时定量PCR技术检测8细胞期和囊胚期胚胎RPL31的mRNA表达量,结果表明,RPL31在8细胞期胚胎的相对表达量为囊胚期胚胎的3.2倍.结论]为揭示和阐明控制牛早期胚胎发育的相关机理提供依据.
Abstract:
The cattle different stage embryos obtained from in vitro was studied using the technology of single preimplantation embryo mRNA different display:single 8-cell and blastocyst stage embryos were studied using technology of mRNA different display and one different fragment was found.The result suggested that this fragment displayed high homology (99%) to cattle mRNA for ribosomal protein L31.Then to detect the expression of RPL31 mRNA in 8 cell and blastocyst stage embryos by real-time quantitative PCR,the result showed the relative amount of 8 cells was 3.2 times of blastocyst's.
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关 键 词: | 体外发育 牛体外受精胚胎 特定阶段 差异表达基因 IVF Gene Expression different mRNA差异显示技术 real-time quantitative PCR technology stage display 早期胚胎发育 细胞 囊胚期 基因表达模式 不同发育时期 表达量 核糖体蛋白 相关机理 |
The Study on the Gene Expression of Preimplantation IVF Bovine Embryos |
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Abstract: | Single preimplantation Embryo mRNA different display Cattle embryo Gene Real-time quantitative PCR |
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Keywords: | Single preimplantation Embryo mRNA different display Cattle embryo Gene Real-time quantitative PCR |
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