| Isolation and Characterization of a Cinnamoyl CoA Reductase Gene（CCR） in Pear（Pyrus pyrifolia） |
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| 作者姓名： | Meng HU Boya TAN Tao WU Xianming LI Junfan TU Fuchen YANG Hongyan ZHU Zhongqi QIN |
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| 作者单位： | [1]College of Life Sciences, Wuhan University, Wuhan 430072, China; [2]Institute of Fruit and Tea, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; [3]Hubei Innovation Center of Agricultural Science and Technology, Wuhan 430064, China |
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| 摘 要： | Pear is a popular and commercially important fresh fruit, and its texture is related to the presence of sclereid formatted by parenchyma cell with lignification in vascular plants. Previous studies have demonstrated that content of lignin may be regulated by cinnamoyl CoA reductase（CCR） in various plants. However, the function of CCR in pears remains very limited. In the present study, we isolated a cDNA encoding CCR（PpCCR, GenBank accession No. KF999958） and its promoter（proPpCCR） from Whangkeumbae pear to investigate the function of CCR in lignin biosynthesis. PpCCR-GFP expressed in rice mesophyll protoplast demonstrated that PpCCR-GFP was localized in the cytoplasm, indicating that CCR may function in cytoplasm without localization signals. In transgenic plants carrying PpCCR, we observed higher lignin content compared with that in wild type plants, further suggesting that PpCCR can affect the lignin contents through regulating lignin biosynthesis in Arabidopsis thaliana. More studies in other plants are needed to confirm our conclusion.
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| 关 键 词： | Pear (Pyrus pyrifolia) Stone cell Lignin CinnamoyI-CoA-reductase |
Isolation and Characterization of a Cinnamoyl CoA Reductase Gene(CCR) in Pear(Pyrus pyrifolia) |
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| Meng HU,Boya TAN,Tao WU,Xianming LI,Junfan TU,Fuchen YANG,Hongyan ZHU,Zhongqi QIN.Isolation and Characterization of a Cinnamoyl CoA Reductase Gene(CCR) in Pear(Pyrus pyrifolia)[J].Agricultural Science ＆ Technology,2014(6):926-932. |
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| Abstract: | Pear is a popular and commercially important fresh fruit, and its texture is related to the presence of sclereid formatted by parenchyma cell with lignification in vascular plants. Previous studies have demonstrated that content of lignin may be regulated by cinnamoyl CoA reductase(CCR) in various plants. However, the function of CCR in pears remains very limited. In the present study, we isolated a cDNA encoding CCR(PpCCR, GenBank accession No. KF999958) and its promoter(proPpCCR) from Whangkeumbae pear to investigate the function of CCR in lignin biosynthesis. PpCCR-GFP expressed in rice mesophyll protoplast demonstrated that PpCCR-GFP was localized in the cytoplasm, indicating that CCR may function in cytoplasm without localization signals. In transgenic plants carrying PpCCR, we observed higher lignin content compared with that in wild type plants, further suggesting that PpCCR can affect the lignin contents through regulating lignin biosynthesis in Arabidopsis thaliana. More studies in other plants are needed to confirm our conclusion. |
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