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利用黑斑病的高抗无性系美洲黑杨I-69及黑斑病的高感无性系欧美杨I-45为材料建立的2个cDNA文库,随机挑取cDNA克隆进行5'端EST序列测序,共获得有效序列20 023条.序列经聚类分析和拼接后,共获得10 816个Unigene,其中包括3 734个Contig,7 082个独立的Singleton.被注释的8 853个具有同源性匹配序列基因中,按照GO的分子功能、生物过程和细胞组分3个不同分类角度进行分类.在具有功能注释的8 853个Unigene中,选出330个与完成全基因组测序的毛果杨序列进行BLAST分析,结果发现有177个抗病相关候选基因出现在282个Unigene中,其中135个分布于杨树的18个不同连锁群上,其他42个基因位于还没定位的scaffolds上.所测定的这些EST序列为后期在基因组水平上研究杨树黑斑病的水平抗性遗传机制及进一步的相关基因发现奠定基础.Abstract: In an attempt to elucidate the molecular mechanism for resistance of black spot disease in poplar,gene expression profiles in leaves of Populus deltoides'Lus'(I-69/55)and P.euramericana'I-45/51',which were inoculated with the pathogen Marssonina brunnea f.sp.brunnea,were analyzed based on expressed sequence tags (ESTs).A total of 20 023 valid ESTs from the 5'terminal ends derived from corresponding cDNA libraries of the two poplar species were sequenced.Cluster analysis of the 20 023 sequences yielded 10 816 tentative unigenes,including 3 734 contigs and 7 082 singletons.All tentative unigenes were classified by Gene Ontology functional categories.To find resistance-associated candidate genes and locate them on poplar genome,330 unigenes was chosen from 8 853 annotated tentative unigenes,and their BLAST alignment was conducted with Populus trichocarpa assembly,1 77 related candidate resistant genes were found,and they presented in 282 unigenes.Among these genes,there were 1 35 genes located on 18 different linkage groups of poplar genome,and 42 genes located on the different scaffolds.This study supplied a resource of candidate genes for further exploring the genetic mechanism for the host horizontal resistance to the pathogen Marssonina brunnea at the whole genome range,and provided important information for further gene discovery. 相似文献
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宜昌市城市森林生态系统建设技术 总被引:2,自引:0,他引:2
从宜昌市自然、社会和经济等信息源入手,按照系统的物质流、能量流、信息流和人口流相互联系的规律,运用城市复合生态观、城市景观生态学等方法,开展城市园林绿地景观类型及空间布局、城市森林水文效益、城市不同绿地类型环境效应等的定性与定量的研究,并通过对宜昌市城市森林生态系统的辩识、诊断、调整与重构等过程,建立一个以成片森林为主,乔、灌、草相结合的复合森林生态系统,达到改善城市生态环境,提高城市生活质量,支持城市持续发展的目的。 相似文献
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以砂生槐种子为材料,利用清水、300mg.L- 1和600mg.L- 1GA3浸泡种子2d、4d和8d,探讨不同处理对砂生槐 种子萌发特性的影响。结果表明,随着浸泡时间的延长,砂生槐种子的发芽率有所提高,而且4~8d的300mg.L- 1 GA3 浸种可显著提高砂生槐种子的发芽率,相比清水浸泡发芽率提高10%~15%。 对于发芽势,清水、300mg.L- 1及600mg .L- 13种处理在浸泡2d和8d后只有 一个发芽势高峰,而浸泡4d后有两个发芽势高峰。 同时,方差分析结果表明,发芽 率在9种不同处理间呈极显著差异,腐烂率呈显著差异,但硬实率在不同处理间没有显著差异。 相似文献
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