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利用黑斑病的高抗无性系美洲黑杨I-69及黑斑病的高感无性系欧美杨I-45为材料建立的2个cDNA文库,随机挑取cDNA克隆进行5'端EST序列测序,共获得有效序列20 023条.序列经聚类分析和拼接后,共获得10 816个Unigene,其中包括3 734个Contig,7 082个独立的Singleton.被注释的8 853个具有同源性匹配序列基因中,按照GO的分子功能、生物过程和细胞组分3个不同分类角度进行分类.在具有功能注释的8 853个Unigene中,选出330个与完成全基因组测序的毛果杨序列进行BLAST分析,结果发现有177个抗病相关候选基因出现在282个Unigene中,其中135个分布于杨树的18个不同连锁群上,其他42个基因位于还没定位的scaffolds上.所测定的这些EST序列为后期在基因组水平上研究杨树黑斑病的水平抗性遗传机制及进一步的相关基因发现奠定基础.Abstract: In an attempt to elucidate the molecular mechanism for resistance of black spot disease in poplar,gene expression profiles in leaves of Populus deltoides'Lus'(I-69/55)and P.euramericana'I-45/51',which were inoculated with the pathogen Marssonina brunnea f.sp.brunnea,were analyzed based on expressed sequence tags (ESTs).A total of 20 023 valid ESTs from the 5'terminal ends derived from corresponding cDNA libraries of the two poplar species were sequenced.Cluster analysis of the 20 023 sequences yielded 10 816 tentative unigenes,including 3 734 contigs and 7 082 singletons.All tentative unigenes were classified by Gene Ontology functional categories.To find resistance-associated candidate genes and locate them on poplar genome,330 unigenes was chosen from 8 853 annotated tentative unigenes,and their BLAST alignment was conducted with Populus trichocarpa assembly,1 77 related candidate resistant genes were found,and they presented in 282 unigenes.Among these genes,there were 1 35 genes located on 18 different linkage groups of poplar genome,and 42 genes located on the different scaffolds.This study supplied a resource of candidate genes for further exploring the genetic mechanism for the host horizontal resistance to the pathogen Marssonina brunnea at the whole genome range,and provided important information for further gene discovery. 相似文献
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‘美洲黑杨’不同家系苗期生长特征比较 总被引:1,自引:0,他引:1
为获得适合长江中下游江汉平原发展的杨树新品种,以优良美洲黑杨无性系为亲本,配制了4个杂交组合,对获得的子代群体无性系化后进行生长性状调查,比较不同家系生长特征和生长节律变化。结果表明:ZS8×2-7的苗高和地径均服从正态分布(P=0.2000.050),2-2×2-46的地径服从正态分布(P=0.0570.050),其余家系的生长性状偏度值在-0.482~0.304之间,峰度值大多在-0.363~0.155之间,接近正态分布,4个家系苗高分布呈左偏态,大于均值的株数较多,地径分布呈右偏态,小于均值的株数相对较多;2个生长性状在家系间均存在显著差异(P0.05),但优势并不明显,苗高均值最大的2-2×2-38比均值最小的ZS8×2-7仅高7.14%,地径均值最大的2-2×2-9比均值最小的ZS8×2-7仅粗7.52%;4个家系从6月到10月的生长节律相近,其中在7月初到7月中旬之间可能出现了夏季休眠。基于各家系生长性状的分布情况,均可开展一定强度的早期选择和淘汰以选择优良无性系。 相似文献
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宜昌市城市森林生态系统建设技术 总被引:2,自引:0,他引:2
从宜昌市自然、社会和经济等信息源入手,按照系统的物质流、能量流、信息流和人口流相互联系的规律,运用城市复合生态观、城市景观生态学等方法,开展城市园林绿地景观类型及空间布局、城市森林水文效益、城市不同绿地类型环境效应等的定性与定量的研究,并通过对宜昌市城市森林生态系统的辩识、诊断、调整与重构等过程,建立一个以成片森林为主,乔、灌、草相结合的复合森林生态系统,达到改善城市生态环境,提高城市生活质量,支持城市持续发展的目的。 相似文献
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