Fertilization rate,motility, lipid peroxidation and pH changes after chilled storage of rainbow trout (Oncorhynchus mykiss) eggs and spermatozoa by a RMPI medium

The aim of this study was to evaluate the effects of Roswell Park Memorial Institute (RPMI) 1,640 medium on chilled storage of eggs and spermatozoa of rainbow trout (Oncorhynchus mykiss). After 3 days of storage, eggs in RPMI 1,640 media (pH 8.2, 9 and 10), Cortland medium and coelomic fluid were inseminated with fresh spermatozoa (Experiment 1). Eggs in RPMI 1,640 medium at pH 8.2 shown the lowest thiobarbituric acid‐reactive substances (TBARS, 0.053 ± 0.003 nmol/ml) and pH changes (from 8.20 ± 0.01 to 8.18 ± 0.01), the highest fertilization rate (82 ± 3%). Undiluted and diluted spermatozoa at ratios of 1:2 and 1:9 with RPMI 1,640 media (pH 8.2, 9 and 10) and Cortland medium were inseminated with fresh eggs (Experiment 2). Spermatozoa in RPMI 1,640 medium at pH 9 (1:9) caused the lowest TBARS content (0.037 ± 0.002 nmol/ml) and pH changes (from 9.00 ± 0.01 to 8.98 ± 0.01), the highest fertilization rate (77 ± 2%) and motility parameters. Based on Experiments 1 and 2, eggs and spermatozoa were stored for another 3 days in RPMI 1,640 medium at pH 8.2 and 9 (1:9) respectively (Experiment 3). Fertilization rate of storage eggs and spermatozoa in Experiment 3 was 79 ± 5%, showing successfully storage of rainbow trout gametes with the same medium.     

河北太行山区典型水土保持林乔木层生物量及碳储量研究

以河北太行山区4种典型水土保持林为研究对象,对混交林(栓皮栎-侧柏)、油松林、栓皮栎林和刺槐林的乔木层各器官生物量、含碳率以及碳储量进行比较研究。结果表明:混交林、油松林、栓皮栎林和刺槐林生物量分别为51.94,86.40,90.19,18.08t/hm^2,栓皮栎林和油松林生物量高于4种水土保持林生物量的均值(61.65t/hm^2),而混交林和刺槐林生物量分别占生物量均值的84.25%,29.33%。不同林分各器官在乔木层生物量中分配顺序均表现为树干>树根>树枝>树叶。4种典型林分各器官含碳率分别为45.16%~58.93%,58.48%~64.61%,51.16%~58.37%,52.35%~62.30%。4种典型林分碳储量为10.10~53.85t/hm^2。不同林分类型各器官碳储量与生物量呈正比关系,与生物量趋势基本相同,碳储量大小表现为油松林>栓皮栎林>混交林>刺槐林。     

电子束技术在竹材仓储中的应用前景

基于电子束技术处理仓储竹材和竹制品，是核技术和林业产业交叉发展的高科技产业。将电子束技术引入竹子仓储加工领域，使辐照代替竹材化学防护剂，辐射能代替热能，可显著降低能源消耗，符合我国节能减排、环境友好等基本国策。文章在总结竹材γ射线辐照改性技术研究的基础上，阐述了“电子加速器及其衍生技术”引入竹材改性领域的优越性，可以替代⁶⁰Co实现辐照加工竹材，解决当前辐照技术在竹质材料领域应用的科学性问题，并提出基于电子束技术的竹材仓储加工和功能性改良发展方向，对于推动竹材仓储加工技术的升级，实现竹材电子束处理技术的高效化、精准化、规模化、市场化，具有深远的科学意义。     

包装对薄皮核桃贮藏品质的影响

以阿克苏干制"温185"薄皮核桃为试材,采用包装结合脱氧剂处理核桃,并以不处理为对照,在常温(20～25℃)条件下贮藏,分别测定过氧化值、酸价、皂化价、碘价、水分含量、脂肪酶、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、脂氧合酶(LOX)、过氧化物酶(POD)和丙二醛(MDA)指标的变化,研究包装对薄皮核桃贮藏品质的影响。结果表明:采用包装结合脱氧剂处理核桃,可有效延缓薄皮核桃过氧化值、酸价及皂化价的升高,抑制碘价和水分含量的下降,并提高SOD和CAT的活性,抑制脂肪酶、LOX及POD活性,延缓MDA含量的上升,保持核桃仁的风味,其中充气(氮气N2)添加脱氧剂包装效果优于其他处理组,对干制薄皮核桃的贮藏效果较好。     

芳香植物纯露处理对鲜切芋头保鲜效果的影响

为了探究芳香植物纯露对鲜切芋头的保鲜效果,本研究观测了迷迭香纯露(RMH)、薄荷纯露(PH)和玫瑰纯露(RH)处理对4℃冷藏鲜切芋头感官品质的影响。其中,RH处理对鲜切芋头褐变的控制效果最好,为了探究其对鲜切芋头褐变的生理机理,探讨了RH处理对苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)活性和总酚含量的影响。结果表明,RMH、PH和RH处理均能有效延缓鲜切芋头硬度的下降、降低病情指数、抑制褐变,表明纯露对鲜切芋头有较好的保鲜效果;同时,RH处理降低了PAL和PPO活性以及总酚含量,表明纯露通过抑制酚类物质的生物合成和氧化代谢而控制鲜切芋头的褐变。本研究为芳香植物纯露的资源化利用和鲜切果蔬保鲜剂的开发提供了新思路。     

Variation in glomalin in soil profiles and its association with climatic conditions,shelterbelt characteristics,and soil properties in poplar shelterbelts of Northeast China

Glomalin-related soil protein(GRSP)sequesters large amounts of carbon and plays important roles in maintaining terrestrial soil ecosystem functions and ecological restoration;however,little is known about GRSP variation in 1-m soil profiles and its association with stand characteristics,soil properties,and climatic conditions,hindering GRSP-related degraded soil improvement and GRSP evaluation.In this study,we sampled soils from 1-m profiles from poplar(Populus spp.)shelterbelts in Northeast China.GRSP contents were 1.8–2.0 times higher in the upper 40 cm soil layers than at 40–100 cm.GRSP-related soil organic carbon(SOC)sequestration in deeper soil layers was*1.2 times higher than in surface layers.The amounts of GRSP-related nutrients were similar throughout the soil profile.A redundancy analysis showed that in both surface and deeper layers,soil properties(pH,electrical conductivity,water,SOC,and soil nutrients)explained the majority of the GRSP variation(59.5–84.2%);the second-most-important factor in GRSP regulation was climatic conditions(temperature,precipitation,and altitude),while specific shelterbelt characteristics had negligible effects(<5%).Soil depth and climate indirectly affected GRSP features via soil properties,as manifested by structural equation model analysis.Our findings demonstrate that GRSP is important for carbon storage in deep soils,regardless of shelterbelt characteristics.Future glomalin assessments should consider these vertical patterns and possible regulating mechanisms that are related to soil properties and climatic changes.     

荔枝果肉热泵干燥及其储藏特性研究

为了探讨荔枝果肉热泵干燥特性及储藏过程中一些品质指标的变化规律，将去皮去核后的果肉进行热泵干燥，测定其干燥特性及储藏过程中色泽、褐变度、总酸、糖组分、质构及微生物指标的变化。结果表明：荔枝果肉在60 ℃下热泵干燥2 h，干燥速度升至最大值后进入降速干燥阶段，干燥过程中，亮度L*值变化不大，a*值和b*值显著增大；干燥18~20 h，样品的水分含量达25%左右、颜色呈金黄色；不同水分含量的荔枝果肉干在储藏过程中，L*值、a*值和b*值下降，果肉发生褐变，总酸含量呈上升趋势，果糖、葡萄糖和蔗糖呈减少趋势；水分含量35%的样品储藏180 d褐变明显；荔枝果干的硬度和咀嚼性在储藏后期明显增大，黏着性变化不显著。采用热泵干燥荔枝果肉，25%水分含量的样品品质和储藏性较佳。     

不同品种茭白贮藏期间的品质及外观商品性变化比较

为筛选出耐贮藏茭白品种和最佳贮藏时间,以6个品种带壳新鲜茭白为试验材料,在(1±1)℃温度下进行贮藏,分析随着贮藏时间的变化,茭白还原糖含量、维生素C含量、粗蛋白含量、粗纤维含量、含水量、表皮色泽等品质及外观商品性变化规律。结果表明,6个供试品种的还原糖含量在整个贮藏期间持续下降且后期下降幅度比前期大;维生素C含量在贮藏1～15 d期间,下降幅度为8.05%～32.06%,贮藏60 d时供试品种的维生素C含量在32.4～34.2mg·kg~(-1)之间;粗蛋白含量在贮藏后期下降幅度比前期大,其中浙茭3号、浙茭6号、浙茭7号和六安茭降幅在40.0%左右;贮藏期间各品种的粗纤维含量均呈上升趋势,其中浙茭3号在贮藏60d时纤维素含量比入贮时增加了127.58%;含水量在贮藏期间保持缓慢下降趋势。综上,贮藏30 d,各供试品种均能保持较好的商品性,但品质均有不同程度的下降;贮藏45 d,浙茭6号、浙茭3号和六安茭还能保持较好的外观商品性,但品质下降较明显;贮藏60 d,只有六安茭还能保持较好的外观商品性,但品质变差。     

不同处理对鲜切莴苣褐变及贮藏品质的影响

以莴苣为研究材料,以10 g/L L-抗坏血酸、5 g/L柠檬酸、0.2 g/L水杨酸和0.2 g/L阿魏酸处理鲜切莴苣,去离子水浸泡为对照组,通过测定褐变度、总酚、类黄酮、多酚氧化酶(PPO)活性、过氧化物酶(POD)活性及苯丙氨酸解氨酶(PAL)活性,探讨不同抗褐变剂对鲜切莴苣褐变的影响。结果表明,4种抗褐变剂处理均能够有效抑制酶促褐变有关酶活性的升高,减少多酚物质的含量,降低褐变度,有效地保证了鲜切莴苣的贮藏品质,其中0.2 g/L阿魏酸处理的效果最好。