GM1 gangliosidosis in a Japanese domestic cat: a new variant identified in Hokkaido,Japan

A male Japanese domestic cat with retarded growth in Hokkaido, Japan, showed progressive motor dysfunction, such as ataxia starting at 3 months of age and tremors, visual disorder and seizure after 4 months of age. Finally, the cat died of neurological deterioration at 9 months of age. Approximately half of the peripheral blood lymphocytes had multiple abnormal vacuoles. Magnetic resonance imaging showed bisymmetrical hyperintensity in the white matter of the parietal and occipital lobes in the forebrain on T2-weighted and fluid-attenuated inversion recovery images, and mild encephalatrophy of the olfactory bulbs and temporal lobes. The activity of lysosomal acid β-galactosidase in leukocytes was negligible, resulting in the biochemical diagnosis of GM1 gangliosidosis. Histologically, swollen neurons characterized by accumulation of pale, slightly granular cytoplasmic materials were observed throughout the central nervous system. Dysmyelination or demyelination and gemistocytic astrocytosis were observed in the white matter. Ultrastructually, membranous cytoplasmic bodies were detected in the lysosomes of neurons. However, genetic analysis did not identify the c.1448G>C mutation, which is the single known mutation of feline GM1 gangliosidosis, suggesting that the cat was affected with a new variant of the feline disease.     

相变材料在绿色储粮中的应用探讨

相变材料能够储存或释放显热,在熔化或凝固过程中虽然温度不变但吸收的潜热却相当大。根据相变材料可以吸收环境热能并在需要时向环境发出热能的特征,研究相变材料在粮食仓储中的应用,减小外界环境对粮仓温度的影响,达到恒温绿色生态储粮的目的。     

江苏徐州市果树经济林的碳储量估算

采用生物量法和重铬酸钾-硫酸氧化法测定全碳含量,对徐州市果树经济林的碳贮量、分布格局进行初步研究。徐州市果树经济林碳储量为39.21×104 t,其中,银杏18.45×104 t、梨3.82×104 t、苹果16.63×104 t、桃2.67×104 t。碳密度为8.7 t/hm2,并分析其空间分布特点。     

婴幼儿配方奶粉在不同贮藏条件下维生素D3稳定性的研究

本文跟踪研究了婴幼儿配方奶粉（简称“婴配粉”）中脂溶性维生素D₃在贮藏过程中稳定性的变化。试验方法：将3 种婴配粉经氢氧化钾乙醇溶液皂化、提取、净化、浓缩后,用正相高效液相色谱柱制备,反相C18高效液相色谱柱分离,经紫外检测器检测,外标法定量检测。试验结果：婴配粉称样量为10.0 g,定容体积为2 mL时,方法检出限为0.303 μg/100 g,方法定量限为0.909 μg/100 g。在此范围内线性回归方程Y=3.3608X+0.0388,此时R²=0.9991。结论婴配粉中维生素D₃的含量在常温和阴凉干燥环境下受到的影响较小;在低温和高温条件下随贮藏时间延长,脂溶性维生素D₃因温度分解含量减少。     

稻谷储藏真菌危害早期预测的研究

对稻谷储藏过程中,水分和温度的变化与真菌生长关系进行了研究,建立了一种稻谷储藏真菌危害早期预测方法.将12.5％％、13.4％、14.5％、15.6％和16.2％水分稻谷样品,置于10℃、15℃、20℃、25℃、30℃、35℃恒温箱中储藏180 d,每10 d取一次样检测真菌生长状况.结果表明:12.5％水分稻谷长期储藏是安全的;13.4％水分稻谷短期储存是安全的,长期储藏仍存在潜在风险;14.5％水分稻谷在15℃以下储存半年是安全的;15.6％和16.2％稻谷在实验的6个温度下储存20 d内均检出有真菌生长.不同温度下稻谷储藏真菌生长顺序为:35℃＞30℃＞25℃＞20℃＞15℃＞10℃.基于上述真菌生长规律的研究,对稻谷储存水分、温度和真菌起始生长时间进行幂函数分析,得出稻谷储存水分、温度和真菌危害早期预测曲线,通过本曲线可进行高水分稻谷储存安全期预测.     

低酚棉子蛋白乳化容量的研究

本文以低酚棉子蛋白为研究材料,采用电导法测定低酚棉子蛋白乳化油脂时的乳化容量。结果表明,低酚棉子蛋白在乳化时其耗油量与蛋白质含量呈线性相关.适合方程y=a+bx,线性回归系数b的值即为低酚棉子蛋白的乳化容量。中性蛋白酶水解低酚棉子蛋白,发现其乳化性能的变化为,酶水解使非贮存蛋白的乳化容量呈下降趋势;酶水解使贮存蛋白在水中的溶解度显著增加,其乳化容量先下降而后又趋上升。     

低温储粮在浅圆仓中的应用及探讨

综合利用冬季粮食入库时的自然低温，采用粮面覆盖密闭等手段控制粮堆温度和仓内温度，可使粮食在低温状态下安全储藏，且成本低廉。     

Limited but specific variations of seed storage proteins in Japanese common wheat (Triticum aestivum L.)

The electrophoretic banding patterns ofgliadin in common wheat lines derived fromJapan were determined byacid-polyacrylamide gel electrophoresis. For the 107 wheat lines used in our study,27 different patterns were identified, 13corresponding to the -gliadin, 8 tothe , -gliadin and 6 to the-gliadin. The gliadin patterns ofJapanese wheat cultivars and landracesgreatly differed from the patterns of wheatlines from other countries, and thevariation seen in wheat lines from Japanwas limited to 46 patterns. Sevencollection or breeding areas in Japanshowed different frequencies in theirgliadin patterns. Combining the gliadinpatterns with high molecular weightglutenin subunit compositions, 67combinations were observed. One gliadinpattern consisting of -gliadinpattern F, , -gliadinpattern H and -gliadin pattern Dwas frequently found in many Japanese wheatlines, though the other patterns werelimited to only one or two wheat lines.     

高大平房仓低温密闭储粮试验

利用冬春季节气温较低的有利时机,对高大平房仓散装小麦进行机械通风,将粮食降到较低温度,然后对粮堆实行隔热密闭并进行低温储粮试验.试验在隔热和防虫方面都取得了较好的效果.试验结果表明,只要对粮堆采取积极有效的隔热密闭措施就能够使粮堆保持较长时间的低温状态,抑制了储粮害虫的孳生繁衍,再采取防止外部害虫感染的措施,就能够实现粮食的元药保管.