羊口疮病毒疫苗株与野毒株VIL-10基因的比较分析

试验旨在比较分析羊口疮病毒（orf virus,ORFV）VIL-10基因在疫苗株和野毒株之间的差异特征。参照GenBank中公布的ORFV NZ2株的VIL-10基因序列设计并合成1对特异性引物,分别以疫苗株和野毒株提取的基因组DNA为模板,采用PCR方法扩增ORFV的VIL-10基因全序列并进行测序,应用生物信息学相关软件分析基因的核苷酸、氨基酸变异情况及蛋白结构。结果显示,本试验测定的疫苗株和野毒株VIL-10基因核苷酸序列同源性为94.4%,差异主要是单个碱基的突变,其中疫苗株在132~134 bp核苷酸序列出现缺失;氨基酸序列同源性为92.5%,出现了15个氨基酸位点的突变,其中疫苗株第42位氨基酸天冬酰胺出现缺失;蛋白质在一级结构及理化性质、二级结构、三级结构、抗原表位参数及有无信号肽之间均存在一定程度的差异,而疫苗株和野毒株编码的蛋白质均无跨膜结构域。系统进化树分析结果表明,本试验测定的野毒株与疫苗株属于不同分支,遗传关系较远。研究结果提示,野毒株与疫苗株的VIL-10基因发生较明显的变异,这些变异可能与ORFV疫苗株的毒力致弱有关。     

Effects of elevated CO2 concentration on the respiratory behavior of the branches of field-grown hinoki cypress (Chamaecyparis obtusa)

The effects of elevated atmospheric CO₂ concentrations on the nighttime respiration were examined for two sample branches of a hinoki cypress tree (Chamaecyparis obtusa) growing in the field with an open gas exchange system for a one-year period from July 1994 to June 1995. The branches were of a similar size and located at a similar position within the crown. One branch was subjected to an elevated CO₂ concentration of 800 μmol mol⁻¹ and the other was subjected to ambient air which had a CO₂ concentration of about 370 μmol mol⁻¹. Nighttime respiration rate was higher in elevated CO₂ level than in ambient CO₂ level. The relationship between nighttime respiration and the corresponding nighttime air temperature was fitted by the exponential function in every month of the year. The segregation of regression lines between the two CO₂ treatments increased gradually as the seasons progressed during the treatment period. TheQ ₁₀ values for nighttime respiration were lower in elevated CO₂ (1.9 ≤Q ₁₀ ≤ 3.7) than in ambient CO₂ (2.4 ≤Q ₁₀ ≤ 4.5) in every month of the year. TheQ ₁₀ was inversely related to the monthly mean nighttime air temperature in both elevated and ambient CO₂. The estimated daily nighttime respiration rate under both CO₂ treatments had a similar seasonal pattern, which almost synchronized with the temperature change. The respiration ratio of elevated CO₂ to ambient CO₂ increased gradually from 1.1 to 1.6 until the end of the experiment. Our results indicate that the CO₂ level and the temperature have a strong interactive effect on respiration and suggest that a potential increase in respiration of branches will occur when ambient CO₂ increases.     

经济供电半径的实用计算方法与分析

基于满足负荷要求及各项技术指标的条件下,以供电范围内单位面积所承担的总费用最小为原则,提出了东北地区６６／１０ｋＶ供电方式的１０ｋＶ配电线路经济供电半径的实用计算方法。同时分析了常规变电站和小型化变电站的合理供电半径,并给出了供电半径与负荷密度之间的函数关系。     

中成药六味地黄丸粉辐照杀菌工艺研究

采用静态堆码方式对中药六味地黄丸粉进行辐照杀菌研究。结果表明,在垂直于辐照源轴线方向不同距离处的药品堆积厚度与辐照剂量率之间呈显著负相关（ ｐ＜ ００１） ,相关系数 ｒ ＝ － ０９９４７ 。辐照工艺技术的改变会不同程度地影响杀菌效果,提高产品总体吸收剂量均匀度,是保证杀菌效果的主要因素。六味地黄丸粉中杂菌的 Ｄ１０ 值为２１７ｋ Ｇｙ 。经１００ ｋ Ｇｙ 剂量辐照后的六味地黄丸粉,没有发生质量改变,其主要药效成分含量没有变化。     

几种有机物对烟草细胞辅酶Q10形成的影响

采用细胞悬浮培养的方法,以烟草红花大金元细胞为材料,研究了几种有机营养物质辅酶Ｑ１０（Ｃｏ１０）合成前体物质及Ｃｏ＾６０－γ射线对烟草悬浮培养细胞生长和辅酶Ｑ１０形成的影响。实验证明,肌醇,酵母膏,盐酸硫胺素对烟草悬浮培养生长和ＣｏＱ１０含量都有一定的影响。     

Production profile and comparison analysis of main toxin components of Fusarium oxysporum f. sp. sesami isolates with different pathogenicity levels

Fusarium wilt is a common fungal disease in sesame caused by Fusarium oxysporum f. sp. sesami (FOS). To determine the toxin production profiles of the FOS isolates with different pathogenicity levels under various culture conditions, we assessed the content variation of fusaric acid (FA) and 9, 10-dehydrofusaric acid (9, 10-DFA) produced by the four representative FOS isolates. Results indicated that the concentration of FA reached to a maximum of 2848.66 ​μg/mL in Czapek medium, while 9, 10-DFA was mainly produced in Richard and Low-carbon Richard medium. The concentration of 9, 10-DFA on Richard culture medium varied from 0 ​μg/mL to 716.89 ​μg/mL. Of the five culture media used in this study, Czapek culture medium was the most conductive to produce FA. FA production was significantly affected by culture medium, culture time, and their interactions. Results suggest that there is no correlation between toxin production and pathogenicity level of FOS isolates. These findings provide key information for the mechanism analysis of FOS- sesame interaction and pathogen control.     

第十届花博会广东“粤芳园”的传统山水造园手法应用

造园,古往今来,神形兼备,“神”通过意境的营造以传达园的精神与韵味,“形”通过地形空间的塑造以形成高低、远近、虚实、藏露的丰富场景,为意境的营造提供平台。神与形相互烘托,互相成就,共同体现了中国传统造园的精髓。第十届花博会广东“粤芳园”通过地形的塑造,营造了“高山流水”“小中见大”的丰富空间与景观层次,以曲径通幽、蜿蜒前行的园径引导游人沉浸式体验绿意盎然、花木繁茂的岭南水乡的风采,在移步换景中感受中国园林的“形”与“神”,体现了传统山水造园手法在当代园林的应用。     

郑州市数码公园不同植物群落对PM2.5和PM10浓度的影响研究

以郑州市数码公园为例,研究不同植物群落对大气颗粒物污染的影响。2020年4月—2021年3月,监测郑州市数码公园内不同植物群落结构(乔灌草、乔草、乔灌、灌草、草坪)在春夏秋冬四季的PM_2.5和PM₁₀浓度以及温度、湿度和风速等气象因子,分析不同植物群落PM_2.5、PM₁₀浓度的变化规律,并研究其与气象因子的相关性。研究结果表明,不同样地内PM_2.5和PM₁₀浓度日变化基本一致,呈早高晚低趋势。不同样地内PM_2.5和PM₁₀浓度均表现为秋冬季高,春夏季低;PM_2.5和PM₁₀浓度高低依次为草坪和广场样地>灌草和乔草结构样地>乔灌草和乔灌结构样地;复合群落结构绿地对颗粒物滞尘能力大于单一群落结构。乔灌草、乔灌结构样地对PM_2.5和PM₁₀的阻滞率较高。PM_2.5和PM₁₀浓度...     

烟草赤星病对不同部位烟叶化学成分的影响

在烤烟调制期分下二棚叶、腰叶、上二棚叶及顶叶4个部位,进行烟草赤星病Alternaria alternata（Fries）Kissler不同危害程度对烟叶化学成分的影响研究。结果表明,受赤星病的危害后,烤烟4个部位烟叶总糖、还原糖含量比同部位对照（0级病害）有减少趋势,总氮、蛋白质及烟碱比同部位对照增加。下二棚叶病害级别与总氮、蛋白质、烟碱含量呈极显著强正相关。腰叶病害级别与烟碱含量呈显著正相关,与总糖、还原糖含量呈显著负相关,与总氮、蛋白质、钾含量呈极显著强正相关,与氯含量呈极显著强负相关。上二棚叶病害级别与总糖含量呈显著负相关,与总氮、蛋白质含量呈极显著强正相关。顶叶病害级别与总糖、还原糖、氯含量呈显著负相关。