类黄酮对蛋鸡生产性能、蛋品质和血液生化特性的影响

试验选用864只39周龄海兰褐蛋鸡,随机分为3个处理,每个处理3个重复,每个重复96只鸡,采用单因素随机试验设计,分别饲喂添加0、30 mg/kg蜂胶黄酮（PF）和6 mg/kg大豆黄酮（DA）的玉米-豆粕型日粮8周。结果表明,日粮中添加PF和DA对蛋鸡产蛋率、破软壳率、料蛋比有改善的趋势;日粮中添加PF和DA能够显著降低蛋黄胆固醇的含量,在蛋保存的第7和14天两者均能不同程度降低蛋黄丙二醛的含量;在试验第4、8周,日粮中添加DA和PF能够显著提高血清HDL水平（P<0.05）,在试验第8周,DA显著降低血清TC和TG水平,而PF显著降低血清TC水平并显著提高血清胰岛素水平（P<0.05）;此外,日粮中添加DA和PF能够显著提高第4周时T、B淋巴细胞转化率（P<0.05）,且添加PF显著提高第8周时T淋巴细胞转化率（P<0.05）。     

蜂胶抗烟草病原真菌的研究

用蜂胶溶液进行体外抑菌实验表明 ,蜂胶对引起烟草赤星病的链格孢菌和引起黑茎病的烟草疫霉菌的真菌有明显的抑制作用     

瑞博·康思农蜂胶片对小鼠细胞、体液、巨噬细胞免疫力影响

以提纯蜂胶为主料、蜂花粉为辅料加工而成的蜂胶片分成低剂量、中剂量、高剂量,对受试动物昆明种小鼠进行试验,小鼠被随机分别分为阴性对照组及低、中、高3个剂量组,每天1次按0.4mg/20g体重,连续30d.研究了蜂胶片对小鼠细胞、体液免疫力的提高效果与巨噬细胞吞噬能力的提高效果,试验结果表明,瑞博·康思农蜂胶片具有明显增强细胞和体液免疫功能的作用,增强巨噬细胞吞噬作用.     

西方蜜蜂不同蜂种及其正反交组合采胶力研究

“浙农大 1号”意蜂 (简称Ea)分别与喀尼鄂拉蜂 (简称K)、卡尔巴阡蜂 (简称C)、高加索蜂 (简称G)、乌克兰蜂 (简称W )、东北黑蜂 (简称D)进行正反杂交组配。在山东荆条场地 ,考察这 6个西方蜜蜂蜂种及 10个正反杂交组合的 15 0个试验蜂群的蜂胶生产性能 ,分析杂交一代的杂种优势。结果表明 :Ea是六个纯种中蜂胶产量最高的蜂种 ,Ea×C是所有纯种和杂交组合中蜂胶产量最高的蜂种 ,是培育蜂胶高产杂交组合的首选蜂种 ,其他 9个杂交组合均未表现出杂种优势     

2018年国内外蜂胶的研究概况

对2018年国内外蜂胶的研究概况进行了综述,对公开发表的212篇中英文文献及2篇硕士学位论文的研究领域、地域分布、刊物分布等情况进行了统计分析和评述,并重点介绍了蜂胶的药理活性、化学成分与蜂胶的质量控制、蜂胶的安全性,以及在农业畜牧业等方面应用的研究新进展,同时也介绍了2018年国内外蜂胶相关专利的公开情况。     

乳化剂对蜂胶抑菌作用的影响

使用几种常用乳化剂对蜂胶乳化后的抑菌作用进行试验，发现乳化后的蜂胶抑菌作用降低近100倍。     

Effects of Propolis Flavone on Antiviral Ability of Host Cell

This experiment was conducted to study the effects of propolis flavone (PF) on antiviral ability of host cell.First,the maximal safe concentration of PF to CEF and PK-15 was determined by MTT method.Then PF was diluted to five concentrations (from 250 to 15.6 μg/mL) with maintenance medium and acceded to foster system of CEF or PK-15 with NDV,IBDV,TGEV and PPV,respectively,and the cell viability was observed by MTT method at 24,48 and 72 h after infection as the index of high antiviral ability of host cell.The results showed that PF could promote the antiviral ability of host cell.And the effects were related to PF concentration,as the concentration increased,the effects were better.The antiviral activity of PF to NDV and TGEV with envelope in host cell existed in infectious earlier stage,while for IBDV and PPV without envelope,the activity appeared in infectious later stage.The results indicated that the antiviral effect period and mechanism of propolis flavone was different for different viruses,propolis flavone could be use for the prevention of NDV and TGEV,and for the treatment of IBDV and PPV.     

小鹅瘟蜂胶灭活疫苗的研究

以蜂胶为佐剂制备小鹅瘟蜂胶佐剂灭活苗,将病毒液灭活后按一定比例加入蜂胶佐剂制备疫苗,并进行疫苗安全性、免疫持续期、效力对比、保存期及区域应用试验,结果表明小鹅瘟蜂胶灭活疫苗安全性好,第5天即产生抗体且抗体持续时间长（90d）,免疫效果远好于油乳剂灭活苗,推广试验也取得了令人满意的结果。     

水溶性蜂胶加工工艺的研究进展

蜂胶具有广泛的生物学活性,但蜂胶在水中的溶解度极低,稳定性差,生物利用度低,因此其应用受到限制。如何使蜂胶均匀地分散于水中,对于蜂胶产品的开发和发挥蜂胶的保健功效有着重要的价值。该文介绍了水溶性蜂胶加工工艺的研究进展,重点介绍了使用乳化剂制备水溶性蜂胶的技术方法,评价了该技术用于蜂胶的适用性和存在的问题,旨在为蜂胶的深入研究和加工利用提供参考。     

Hepatic endogenous defense potential of propolis after mercury intoxication

Exposure to mercuric chloride (HgCl₂; 5 mg kg^?1 body weight; i.p.) induced oxidative stress in mice and substantially increased lipid peroxidation (LPO) and oxidized glutathione (GSSG) levels, decreased the level of reduced glutathione (GSH) and various antioxidant enzymes in liver and also increased the activities of liver marker enzymes in serum. Therapy with propolis extract, a resinous wax‐like beehive product (200 mg kg^?1 orally, after mercury administration), for 3 days inhibited LPO and the formation of GSSG and increased the level of GSH in the liver. Release of serum transaminases, alkaline phosphatase, lactate dehydrogenase and γ‐glutamyl transpeptidase were significantly restored after propolis treatment. The activities of antioxidant enzymes, that is, superoxide dismutase, catalase, glutathione‐S‐transferase and glucose‐6‐phosphate dehydrogenase, were also concomitantly restored towards normal levels after propolis administration. These observations clearly demonstrate that propolis treatment augments antioxidant defense against mercury‐induced toxicity and provide evidence that propolis has therapeutic potential as a hepatoprotective agent.