高压均质法制备O/W型蜂胶纳米乳液的研究

以蜂胶醇提物为原料,橄榄油为食品级油相,吐温80为表面活性剂,无水乙醇为助表面活性剂,在确定最佳配比的基础上制备O/W型蜂胶纳米乳液,研究了不同均质压力参数（0,40,80 MPa）设定条件下乳化液的平均粒径与分布情况。研究结果表明,均质压力对乳化液的平均粒径与分散指数具有显著影响,经80 MPa高压均质处理所得的乳化液粒子平均直径为（1432±1.4）nm,且粒径分布均一,具有较好的稳定性。     

Effects of dietary propolis and vitamin E on growth performance and antioxidant status in juvenile rainbow trout (Oncorhynchus mykiss) under different flow rate

The present study investigated the effects of propolis and vitamin E supplementation in diets of juvenile rainbow trout subjected to two different flow rates (0.9 and 2.1 L min^?1) on growth performance, and vitamin A, C and E concentrations in tissues as well as malondialdehyde (MDA) levels. Juvenile rainbow trout were fed with diets containing 10 and 30 g propolis kg^?1, 60 mg kg^?1 vitamin E (Rovimix E‐50 adsorbate; min.%50 dl‐α‐tokopherly acetate) and without supplemented basal diet for 12 weeks. Weight gain (WG) in the C group was significantly lower (P < 0.05) than P10, P30 and E60 groups at both flow rate treatments. At 2.1 L min^?1, specific growth rate (SGR) in the C group was significantly lower (P < 0.05) than other groups, but at 0.9 L min^?1, SGR of fish did not differ among the diets groups (P > 0.05). Survival rate (SUR) was higher in propolis and vitamin E supplemented diet groups (P < 0.05) than control diet group at 0.9 L min^?1. Fish fed on diet E60 had higher (P < 0.05) tissue vitamin E concentration than fishes fed on other diets groups. Vitamin C concentration in rainbow trout tissues was significantly affected by the 30 g propolis supplemented diet group (P < 0.05), followed by the 10 g propolis supplemented diet group (P < 0.05). MDA level of E60 group was found significantly decreased instead of different than other groups (P < 0.05). The results of Student's t‐test revealed that WG, SGR, SUR values, vitamin (A, C, E) concentrations and MDA levels of tissues were negatively affected by 0.9 L min^?1flow rate treatment in juvenile rainbow trout.     

不同工艺提取的蜂胶对2型糖尿病大鼠降血糖作用比较

探讨了采用乙醇提取和超临界萃取的2种蜂胶的降血糖作用,并进行比较研究.将大鼠随机分组后连续9周灌喂蜂胶,每2周测空腹血糖和餐后血糖;于第9周进行糖耐量试验;处死大鼠后,测定其血液中甘油三脂、胆固醇、尿酸、肌酐、尿素氮的含量;解剖大鼠后分别称取其肝、肾重量.结果表明造模后试验组的终重与正常组的差异显著;超临界试验组的糖耐量和胆固醇指标与模型1组的差异显著;其他各项指标的差异均不显著.     

蜂胶对金黄色葡萄球菌α-溶血素的抑制作用

通过吸光度测量和荧光定量PCR的方法,在体外检测亚抑菌浓度的蜂胶作用下金黄色葡萄球菌α-溶血素活力变化及agrA、hla的mRNA表达量。结果显示,不同浓度蜂胶对金黄色葡萄球菌α-溶血素有不同程度的抑制作用（P〈0.05）;同时荧光定量PCR检测agrA和hla基因表明,蜂胶能够显著减少agrA和hla mRNA的相对表达量,进而抑制α-溶血素的表达。     

云南蜂胶化学成分研究(Ⅱ)

研究了从云南蜂胶中获得的3个化合物,经波谱分析(1HNMR、13CNMR、EI-MS)为球松素、白杨素和咖啡酸。3个化合物均为蜂胶中的活性物质,其中球松素从云南蜂胶中首次分离得到。从而进一步阐明了云南蜂胶的药用价值以及为开发利用蜂胶资源提供了新的实验依据。     

奶牛乳头外用蜂胶保护膜的研制

为了改善由于抗生素的长期应用,各种细菌对其产生了很强的耐药性的状况,试验利用蜂胶、聚乙烯醇(PVA)17-88、PVA05-88、吐温80、聚乙二醇、氮酮、丙二醇、东蓑若碱、白及等制备奶牛乳头外用蜂胶保护膜,以便能将其应用于奶牛乳房疾病的治疗与预防.调节基质的pH值,可控制成膜的时间与性状.实验结果表明,pH值均在5....     

鸽新城疫(ND)蜂胶佐剂灭活疫苗免疫效果对比试验

采用鸽新城疫ND-GS 01株毒为种毒,分别研制出了尿囊液毒蜂胶佐剂灭活疫苗和四元材料(鸡胚尿囊液、羊水、尿囊膜、羊膜)混合病毒蜂胶佐剂灭活苗。对研制的两种灭活疫苗经安全性检验、急性毒性试验,免疫抗体消长水平与免疫持续期对比试验,结果表明,四元材料的疫苗与尿囊液疫苗性能一致。     

气相色谱-质谱法分析蜂胶与杨树胶化学组分及鉴别研究

以气相色谱-质谱联用仪分别对蜂胶和杨树胶的化学组分进行了分离鉴定,共鉴定出75种物质。初步确定两种样品中2,6-二羟基-4-甲氧基查耳酮、高良姜素和柚木柯因的含量有明显差异,而且仅在杨树胶中发现了愈创木醇、1-甲基-4-(6-甲基-5-庚烯-2-基)苯、2-苯基苯并咪唑。结果表明,可将上述物质作为蜂胶中的标志性物质进行检测与辨别。     

蜂胶对淋巴细胞调节作用的研究进展

综述了蜂胶对特异性免疫系统影响的国内外研究进展,特别是蜂胶对抗体和淋巴因子分泌、淋巴细胞增殖和分化的调控.结果表明:蜂胶可促进抗体的产生,促进免疫低下的机体和接种疫苗或肿瘤细胞的机体淋巴细胞的增殖,增强机体免疫力;蜂胶能调节淋巴细胞分化和淋巴因子的产生,抑制淋巴细胞的增殖,与其抗炎特性相关.