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排序方式: 共有5143条查询结果,搜索用时 15 毫秒
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Detection of challenge virus in fetal tissues by nested PCR as a test of the potency of a porcine parvovirus vaccine 总被引:1,自引:0,他引:1
Belák S. Rivera E. Ballagi-Pordány A. Hanzhong W. Widén F. Soós T. 《Veterinary research communications》1998,22(2):139-146
To estimate the potency of a porcine parvovirus (PPV) vaccine, three vaccinated and three non-vaccinated pregnant gilts were infected with PPV and the distribution of the virus was studied in the tissues of their 51 fetuses. Virus detection was attempted using haemagglutination (HA) and immunofluorescence (IF) assays, as well as by standard (single) and nested polymerase chain reactions (PCR). None of the detection methods yielded positive results when used to test for the presence of virus in suspensions of organs from the fetuses from the vaccinated gilts. However, the virus was detected in the fetuses from non-vaccinated gilts as follows: HA was positive in 14 cases out of 23 (60.8%), IF in 16/23 (69.5%), standard PCR in 12/20 (60%), and the nested PCR in 19/23 (82.6%). Although the correlation among the results of various methods of virus detection was rather close (r<0.83), the sensitivity of the nested PCR was the highest, both when testing dilutions of PPV and when analysing the fetal organs. The nested PCR therefore provides a reliable approach for studies of virus distribution in fetal organs, with special reference to potency tests on vaccines. 相似文献
176.
Preliminary evidence for the presence of Helicobacter-like bacteria was sought in 395 porcine gastric samples by a urease test. Of the samples, 37% (146/395) were urease-positive and 82% (82/100) of the Gram-stained urease-positive samples showed large, tightly spiralled organisms. Several methods were applied to culture the organisms but isolation was unsuccessful, contaminant organisms being considered to be one of the major problems. PCR with Helicobacter genus-specific primers for 16S rRNA and ureB genes, and primers for H. pylori vacA and cagA genes were tested with 102 urease-positive biopsy samples. The PCR results showed some evidence for the presence of the urease and the vacA genes in porcine Helicobacter-like bacteria and raises the possibility of pathogenicity by these organisms. 相似文献
177.
土霉素注射液在猪体内药代动力学比较 总被引:6,自引:5,他引:1
对长效土霉素注射液进行了猪体内药代动力学的比较研究。试验将三种产品(A、B、C)分别一次肌肉注射猪3头,剂量20mg/kg。结果表明,消除半衰期以产品B最长,血药峰浓度以产品B最高,达到峰浓度时间以产品A最慢,药时曲线下面积以产品B最大,24小时血药浓度以产品B最高,48小时血药浓度以产品C最低。以血浆土霉素浓度0.5μg/ml为最小有效浓度,产品A和B能够维持有效血药浓度24小时,产品C仅能够维持有效血药浓度12小时。 相似文献
178.
为了探讨在自体移植的情况下,每头受体移入的微注射基因胚胎的数量对猪基因导入总效率的影响,在导入PEFhDAF(Humandecayacceleratingfactor,人类补体衰减加速因子)基因的实验过程中,将受体母猪按称入胚数分成三组:第一组12~15枚,第二组16~20枚,第三组21~32枚,其受孕率分别为80%,90.9%,和70%,三组之间的差异不显著(P〉0.05);共产仔率分别为19. 相似文献
179.
ULTRASONOGRAPHIC DETECTION OF ADRENAL GLAND TUMOR AND URETEROLITHIASIS IN A GUINEA PIG 总被引:1,自引:0,他引:1
Lorrie Gaschen DVM Cornelia Ketz Dr. med. vet. Johann Lang Dr. med. vet Urs Weber Dr. med. vet. L. Bacciarini Dr. med. vet. Ingrid Kohler med. vet. 《Veterinary radiology & ultrasound》1998,39(1):43-46
A 5-year-old guinea pig was presented to the University of Berne Small Animal Radiology Department for an ultrasound examination of the abdomen to confirm a suspected diagnosis of Cushing's syndrome. The patient had bilateral alopecia, was apathic and obese. Ultrasonographically, a tumor of the left adrenal gland, obstruction of the left ureter by an ureterolith, as well as hydronephrosis of the left kidney were detected. During surgery to relieve the ureteral obstruction the adrenal gland tumor was removed. The guinea pig died post-operatively due to blood loss. The left adrenal gland tumor was found histopathologically to be an adenoma and the right adrenal gland also had multiple small adenomas, but grossly appeared normal. The ureterolith was analyzed and found by x-ray diffraction to consist of calcium carbonate. 相似文献
180.
松辽黑猪生长发育性能研究 总被引:2,自引:0,他引:2
[目的]为松辽黑猪新品系和吉松配套系选育优质肉猪新品种提供理论依据。[方法]选取松辽黑猪,松野杂猪和杜长大三元杂交育肥猪各15头,分成3个组分别为S、Y和B组,组内设3个重复,每个重复5头猪,在同一条件下进行饲养试验,并对其生长发育性能进行测定。[结果]S组松辽黑猪的胸围发育程度明显高于Y组松野杂猪和B组杜长大三元杂交猪,差异极显著(P〈0.01),其他指标均差异不显著;Y组松野杂猪的增重速度、发育程度、屠宰率和产瘦肉率与B组杜长大三元杂交猪相比,差异均不显著。[结论]在增重速度和产肉率方面,松辽黑猪新品系和松野杂猪新品种的选育是成功的。 相似文献