杀菌剂啶菌噁唑的高效液相色谱分析

建立了一种用反相高效液相色谱测定啶菌噁唑的定量分析方法。采用C18ODS色谱柱,以甲醇-水(75∶25,体积比)作为流动相,流速1.0mL/m in,检测波长220nm。啶菌噁唑色谱图显示两个吸收峰,分别为两个同分异构体Z体和E体。在400700μg/mL浓度范围内,标准溶液浓度和吸收峰面积之间有良好的线性关系,回归方程为Y=4.846 7x+232.85,相关系数r为0.999 2;对原药溶液及其制剂溶液测定的重复性SR分别为0.004 0和0.004 7。该方法准确、精密,适用于啶菌噁唑的定量分析。     

抗球虫中药复方TF-103指纹图谱的建立

采用高效液相色谱法(HPLC)建立了新型抗球虫中药复方TF-103的指纹图谱。实验采用Dia-monsil C18色谱柱(250mm×4.6mm5μm i.d.);流动相:A相为0.2%磷酸和0.1%三乙胺的水溶液,B相为乙腈,梯度洗脱;柱温:30℃;流速:1.0ml/min;紫外检测波长:330nm。以内标物香兰素、总峰面积以及峰面积最大的9个共有峰为评价指标,并采用“中药色谱图分析和数据管理系统”软件进行了相似度计算。结果表明,该法操作简单,精密度和稳定性良好,有助于该中药复方的质量控制和谱效关系的研究。     

金耳发酵液对糖尿病小鼠降血糖作用的研究

研究表明，金耳发酵液对四氧嘧啶诱导的糖尿病小鼠有降血糖作用，同模型对照组相比．大剂量组差异显著（P〈0.05）；同时，大剂量组对糖尿病小鼠摄食、饮水量的减少及体重的增加均有显著影响（P〈0.05）     

Treatment of cow-waste slurry by a microbial fuel cell and the properties of the treated slurry as a liquid manure

Resource recycling and the proper treatment of animal waste to reduce its environmental impact are currently important issues for the livestock industry. A microbial fuel cell (MFC), a new type of bioreactor, is expected to play roles in both waste‐water purification and energy recovery. However, the generation of electricity from cow waste has not yet been examined. In this study, using an MFC, we examined the possibility of generating electricity from dairy‐cow waste slurry, and analyzed the properties of the treated slurry as liquid manure for resource recycling. The MFC treatment of the slurry generated electricity in a dose‐dependent manner, and the maximum power output by the MFC from a 1 g of chemical oxygen demand/L slurry was 0.34 mW/m². After the MFC treatment, 84% of the biological oxygen demand in the slurry was removed and three essential fertilizer elements (nitrogen, phosphorus, and potassium) were retained at 84, 70, and 91% levels, respectively. The amount of ammonia nitrogen in the slurry, as an element of fast‐release fertilizer, was increased by 1.9‐fold. Although the treated slurry displayed properties that made it preferable as liquid manure, further studies to improve the electrical power output by the MFC are required for practical use.     

猪苓菌丝体多糖对小鼠免疫水平的影响

通过人工液体浅层培养方式培养猪苓菌丝体，用热水浸提法提取猪苓菌丝体多糖（PPS1），经纯化测定其糖含量，用红外光谱分析鉴定多糖，纯化鉴定的PPS1，通过小鼠进行腹腔单核巨噬细胞功能测定试验、E玫瑰花环试验、足跖肿胀厚度试验、淋巴细胞转化试验、EAC花环试验等5项免疫学试验初步探讨其免疫药理学作用，同时与猪苓菌核多糖（PPS2）进行比较。试验数据经统计学分析显示，E玫瑰花环试验中PPS1与空白对照组相比差异显著（P〈0．05）；腹腔单核巨噬细胞功能测定试验、足跖肿胀厚度试验、淋巴细胞转化试验覆EAC花环试验PPS1与空白对照组相比差异极显著（P〈0.01）；5项免疫学试验PPS1与PPS2相比差异均不显著。结果表明，PPS1能明显提高小鼠的免疫功能。     

2种保鲜方法对脐橙果实风味和色泽变化的影响

以赣南纽荷尔脐橙冷藏和留树保鲜果实为材料,进行品质分析,并运用HPLC技术分析果皮与果肉的类胡萝卜素组成和色泽变化。结果表明,冷藏果实固酸比(17.08)比对照(21.36)降低了4.28;而留树保鲜果实固酸比增加到33.33,约为冷藏果实的2倍;冷藏果实在缺乏光照条件下仍可合成类胡萝卜素,尤其是β-隐黄质和玉米黄质,但是果皮偏黄色,可能是由类胡萝卜素的组成、比例及空间分布的改变引起的;留树保鲜类胡萝卜素的合成积累约为冷藏的1.5倍,果皮呈现鲜艳的橙色,是紫黄质和(9Z)-紫黄质大量积累的结果。     

Cryopreservation of carnation (Dianthus caryophyllus L.) shoot tips by encapsulation-vitrification

Shoot tips excised from in vitro cultured plants of Dianthus caryophyllus L. (cv. Pallas, cv. Pink Candy and cv. Wanessa) were successfully cryopreserved using an encapsulation-vitrification method. Shoot tips (2–3 mm in length) were encapsulated in sodium alginate, precultured on liquid Murashige and Skoog (1962) medium supplemented with various sucrose concentrations (0.25, 0.5, 0.75, 1.0 M) for 24 h or 48 h and dehydrated with the vitrification solution PVS2 (up to 4 h) at 24 °C or 0 °C prior to direct immersion in liquid nitrogen (−196 °C). A maximum of shoot regeneration from cryopreserved shoot tips was obtained with the following combinations: preculture in 0.5 M sucrose and 180 min dehydration treatment at 0 °C for cv. Pallas (60% shoot formation), or preculture in 0.75 M and 200 min dehydration at the same temperature for cv. Pink Candy (66.6% shoot formation) and cv. Wanessa (73% shoot formation).     

不同装液量对金顶侧耳液体摇瓶培养的影响

以食用菌金顶侧耳为试验研究对象;用综合PDA(马铃薯20 g,葡萄糖20 g,硫酸镁1.5g,磷酸二氢钾3.0g,水1 000 mL,pH自然)为液体培养基;以(25±1)℃,180 r/min为气浴恒温振荡器的温度和振荡速率.设定了60、80、100、120、140 mL 5个不同的装液量.经过7 d的摇瓶培养,过滤发酵液,得到菌丝体和粗酶液.再通过测定菌丝体烘干后的生物量,以及测定粗酶液中多酚氧化酶和漆酶活性,得出了:装液量为100 mL,菌丝体烘干生物量明显大于其他装液量;装液量为140 mL,有利于多酚氧化酶的分泌,酶的活力较强,并且其活力随装液量的递增而变强.装液量为100mL,有利于漆酶的分泌,酶的活力较强,不同装液量的菌丝生长都在第6天出现了高峰期,其中以装液量为100mL/250mL时,其菌丝球数量较多.     

淀粉废液发酵香菇菌丝体

作者以淀粉废液为主要碳源,运用正交试验,采用摇瓶发酵筛选香菇液体发酵培养基。结果显示,在以淀粉废液为主要碳源的培养基中,每升含玉米浆１０ｇ,甲基纤维素１０ｇ,碳酸镁８．８ｍｇ和硫酸亚铁５ｍｇ最适宜于香菇菌丝生长发育。在诸因素中,甲基纤维素最重要。提示香菇液体发酵菌丝球的数量与大小,除与菌种、营养成分有关外,还与培养基的粘度（Ｖｉｓｃｏｓｉｔｙ）相关。添加适量的甲基纤维素能使香菇菌丝球变小,均匀而菌丝量增加。     

落叶松播种苗使用钼酸铵根外追肥试验报告

通过对落叶松播种苗根外追施钼酸铵液，促进苗高，地径生长，较明显地提高了苗木整体质量，对苗木木质化和提高苗木对不良环境的抵抗力有着积极的促进作用。