猪白细胞介素-6基因的非融合原核表达研究

用DNA重组技术，将已克隆的猪IL-6基因阅读框片段插入非融合原核表达质粒pBV220中的适当位置，获得重组质粒pBVpIL-6，转化大肠杆菌DH5α后，42℃诱导阳性表达菌。经SDS-PAGE检测发现目的蛋白获得了高效表达，表达量约占总蛋白的20％。表达的蛋白以包涵体形式存在，包涵体经过变性溶解和复性后用酶联免疫吸附试验（ELISA）做定性定量检测。ELISA结果证实了所表达的蛋白为猪IL-6，复性后具有一定活性．并测出了复性后活性蛋白的浓度。     

银杏叶类胡萝卜素的研究

银杏叶和提取黄酮(GBE)生产过程中的废弃物含有的类胡萝卜素主要为叶黄素、紫堇新质、隐黄质、新黄质和其他胡萝卜素,是天然的着色剂,如能加以利用,是银杏产业资源综合利用和循环经济发展的途径之一。     

谷氨酰胺对高温下黄羽肉鸡增重及血液生化指标的影响

选用42日龄健康“岭南黄”黄羽肉鸡60只,饲养于32±2℃的封闭舍内,随机分成4组,A组为对照组,饲喂基础日粮,B、C和D组为试验组,分别在基础日粮中添加0.2%、0.5%和0.8%的谷氨酰胺,试验期14 d。试验结果表明,高温下在日粮中添加谷氨酰胺能提高黄羽肉鸡的平均日增重,其中添加0.5%谷氨酰胺的效果显著(P<0.05),其显著提高49、56日龄的血清总蛋白含量(P<0.05),添加0.2%谷氨酰胺显著降低56日龄的血清尿素氮含量(P<0.05),添加谷氨酰胺对血清中的葡萄糖含量无影响(P>0.05)。说明谷氨酰胺有缓解肉鸡高温应激的作用。     

一类p-Laplacian方程非平凡解的存在性

用山路引理得到了一类带有Dirichlet边值条件的p-Laplacian方程的非平凡解的存在性及多解性.     

Effects of Carbon Dioxide Insufflation Combined With Changes in Body Position on Blood Gas and Acid-Base Status in Anesthetized Llamas (Llama glama)

Objective— To study the combined effects of intra-abdominal CO₂ insufflation with changes in body position during laparoscopy in xylazine-ketamine-halothane anesthetized llamas. Study Design— Prospective, controlled study. Animals— Nine castrated, male llamas weighing 114 ± 23 kg, 3 to 13 years old. Methods— Three llamas (preliminary study [PS] group) were used to study the effect of right lateral, dorsal, and left lateral recumbency on gas exchange and acid-base status. The other six (experimental study [ES] group) were used to study the combined effects of changes in body position and CO₂ insufflation to an intraabdominal pressure of 10 to 12 mm Hg. Heart rate, respiratory rate, and indirect arterial blood pressures (systolic [SAP], mean [MAP], and diastolic [DAP]) were recorded every 5 minutes during anesthesia. Arterial blood gases (PaO₂ and PaCO₂) and acid-base status (pHa and HCO₃) were measured immediately after induction of anesthesia and before each change of position. Results— In the PS group, significant decreases in SAP, MAP and PaCO₂ and increases in PaO₂ and pHa were observed when the llamas were turned from right lateral to dorsal recumbency. Values for HCO_-3 were lower than the postinduction values, but they remained unaffected by the changes in position. In the ES group, values for MAP were significantly lower when the llamas were placed in dorsal and left lateral recumbency than those observed during right lateral recumbency. Arterial O₂ tension during right lateral recumbency was lower but returned to preinsufflation values when the llamas were placed in the dorsal position. All llamas recovered uneventfully within 30 minutes after termination of anesthesia. Conclusions— Insufflation of CO₂ and changing body position induce minor and transient changes in cardiovascular and respiratory function. Clinical Relevance— Laparoscopy with mild intra-abdominal CO₂ insufflation (10 to 12 mm Hg) can be used safely in spontaneously breathing llamas anesthetized with xylazine, ketamine, and halothane.     

用套式PCR方法对几种猪用冻干疫苗的PCV2检测

参照Genbank已发表的猪圆环病毒2型的基因序列,取其比较保守的基因片段ORF2,利用引物设计软件Oli-go5.0设计两对PCV2型特异的引物,其中第一对引物扩增跨度为ORF2全基因片段,长度为702 bp,第二对引物扩增ORF2中间的一个小片段,跨度大小为494 bp.首先用第一对引物扩增阳性DNA,阳性DNA的浓度从10-1稀释到10-11,然后以第一次PCR产物为模板,用第二对引物进行PCR,发现这种套式PCR的方法比用普通PCR灵敏度提高102倍.同这种套式PCR方法对广东省市场上出售的几种猪用弱毒疫苗进行猪圆环病毒2型检测,结果发现这几种疫苗全部为阴性,本实验说明目前广东市场上出售的弱毒疫苗在制作过程中没有受到PCV2的污染,解除了广大猪场主的顾虑.     

Basic fibroblast growth factor and Alzheimer's disease

Alzheimer's disease (AD), which is one type of senile deimentia,has three remarkable pathologic characteristics-senile plaque, neurofibrillar tangles and neuronal death. As a neurotrophic factor, basic fibroblast growth factor (bFGF) is closely related to AD. This art icle reviewed the interaction between bFGF and AD-associated gene products (such as amyliod protein, presenilin, apoloprotein E and microtubulin-associated protein tau) and neuronal apoptosis. Some researchers suggested that bFGF may be put forward as potential therapeutic agent in AD and play a role in preventing or retarding the pathological process of this disease, alleviating or even eradicat ing the pathological insults of this disease.     

尤溪县林业分类经营改革试点的实践与启示

通过对尤溪县的三个具有代表性试点单位进行林业分类经营改革实践 ,探索出一套既科学又可操作的林业分类经营改革作法 ,为推进林业分类经营改革提供借鉴经验。目前 ,这些做法已在尤溪全县林业分类经营改革中得到应用     

几种中药及营养物质抗寒作用的研究进展

寒冷是高寒地区动物和人的一种常见应激原。急性（短期）暴露于寒冷环境的动物将产生冷应激（Cold stress）反应。适当的应激反应对机体适应各种刺激因素的作用，维护自身平衡和稳定是有益的，但过强或长期的应激则会给机体带来严重危害。本文对近期有关中药和营养物质抗寒作用方面的研究做了综述。     

西藏砂生槐种子萌发特性研究

自然条件下藏砂生槐种子萌发比较困难，不同收获年份砂生槐种子发芽率不同，砂生槐种子的休眠由种皮障碍所致，物理处理比化学处理发芽率高，最佳处理为刀刺破种皮及砂布磨擦种皮发毛（用具消毒），发芽率可达90％和83％。