围网与普通网箱养殖大黄鱼营养成分的比较与分析

本实验采用临近海区围网与普通网箱养殖的大黄鱼来研究其肌肉营养成分（包括常规营养成分和游离氨基酸）的含量与营养评价。结果表明：围网养殖大黄鱼的粗蛋白、氨基酸总量、呈味氨基酸、鲜味氨基酸含量明显高于普通网箱（P〈0．05）,其必需氨基酸、非必需氨基酸总量亦高于普通网箱;其粗脂肪含量则显著低于普通网箱（P〈0．05）;而粗灰分与水分含量二者没有显著性差异（P〉0．05）。围网养殖的大黄鱼肌肉营养成分明显高于普通网箱,通过改变养殖模式如嗣网养殖可作为提高大黄鱼肉质的一种可行途径。     

蓬莱玉参(Apostichopus sp.)体壁的营养成分分析及评价

采用生化方法对蓬莱玉参(Apostichopus sp.)体壁营养成分进行了测定,并分析评价了这一水产养殖新品种的营养和开发价值。结果显示,蓬莱玉参体壁中水分、粗蛋白、粗脂肪和灰分含量分别为91.29%、5.25%、0.23%和3.09%,其中,粗蛋白质和灰分高于刺参,粗脂肪低于刺参,具有高蛋白、低脂肪的特点;蓬莱玉参体壁中氨基酸总量为53.76%,明显高于刺参,氨基酸组成和含量接近于FAO/WHO的推荐模式,属于质量较好的蛋白质;蓬莱玉参体壁中呈味氨基酸含量为25.63%,占氨基酸总量的47.66%,且各种呈味氨基酸含量均高于刺参,鲜美程度要好于刺参。蓬莱玉参体壁中多不饱和脂肪酸含量(PUFA)为26.47%,DHA+EPA为12.08%,不饱和脂肪酸与饱和脂肪酸的比值(UFA/SFA)为3.19,均高于刺参,因此,脂肪质量优于刺参;蓬莱玉参体壁中常量及微量元素含量较高,Ca、P比例适宜,并富含VB6和VB1,其中,VB1含量为1.30 mg/100 g,含量远高于刺参。研究结果表明,蓬莱玉参营养价值优于刺参,具有较高的营养价值和保健作用。     

蓬莱玉参(Apostichopus sp.)体壁的营养成分分析及评价

采用生化方法对蓬莱玉参(Apostichopus sp.)体壁营养成分进行了测定,并分析评价了这一水产养殖新品种的营养和开发价值。结果显示,蓬莱玉参体壁中水分、粗蛋白、粗脂肪和灰分含量分别为91.29%、5.25%、0.23%和3.09%,其中,粗蛋白质和灰分高于刺参,粗脂肪低于刺参,具有高蛋白、低脂肪的特点;蓬莱玉参体壁中氨基酸总量为53.76%,明显高于刺参,氨基酸组成和含量接近于FAO/WHO的推荐模式,属于质量较好的蛋白质;蓬莱玉参体壁中呈味氨基酸含量为25.63%,占氨基酸总量的47.66%,且各种呈味氨基酸含量均高于刺参,鲜美程度要好于刺参。蓬莱玉参体壁中多不饱和脂肪酸含量(PUFA)为26.47%,DHA+EPA为12.08%,不饱和脂肪酸与饱和脂肪酸的比值(UFA/SFA)为3.19,均高于刺参,因此,脂肪质量优于刺参;蓬莱玉参体壁中常量及微量元素含量较高,Ca、P比例适宜,并富含VB6和VB1,其中,VB1含量为1.30 mg/100 g,含量远高于刺参。研究结果表明,蓬莱玉参营养价值优于刺参,具有较高的营养价值和保健作用。     

青、白刺参(Apostichopus japonicus)体壁营养成分的比较分析

通过对相同养殖环境条件下,青、白刺参(Apostichopus japonicus)体壁中营养成分(粗蛋白、粗脂肪、多糖、脂肪酸、氨基酸、微量元素等)的测定,比较两种刺参的营养品质与价值。结果显示,青、白刺参的出皮率,体壁中的水分、多糖和灰分含量差异显著(P0.05),粗蛋白和粗脂肪差异不显著(P0.05)。青、白刺参体壁中均检测出20种主要脂肪酸,脂肪酸总量和多不饱和脂肪酸(PUFA)的含量差异不显著(P0.05),而青刺参饱和脂肪酸(SFA)含量显著高于白刺参,单不饱和脂肪酸(MUFA)含量显著低于白刺参(P0.05)。MUFA中青刺参的花生四烯酸(AA)和二十二碳六烯酸(DHA)含量均显著低于白刺参(P0.05)。青、白刺参体壁中检测出17种氨基酸,必需氨基酸(EAA)、鲜味氨基酸(FAA)和药效氨基酸(DAA)的含量均无显著差异(P0.05)。青刺参体壁中天冬氨酸和精氨酸含量均显著低于白刺参(P0.05)。青刺参体壁中Mn和Cr的含量显著低于白刺参(P0.05)。重金属元素Pb、Cd、Hg、As的含量均符合相关食品卫生标准。综合评价认为,相同养殖环境条件下,白刺参的出皮率、多糖含量、脂肪酸中不饱和脂肪酸含量、AA和DHA的相对百分含量、氨基酸中天冬氨酸和精氨酸含量以及微量元素中Mn和Cr含量均优于青刺参。     

青、白刺参(Apostichopus japonicus)体壁营养成分的比较分析

通过对相同养殖环境条件下,青、白刺参(Apostichopus japonicus)体壁中营养成分(粗蛋白、粗脂肪、多糖、脂肪酸、氨基酸、微量元素等)的测定,比较两种剌参的营养品质与价值.结果显示,青、白刺参的出皮率,体壁中的水分、多糖和灰分含量差异显著(P＜0.05),粗蛋白和粗脂肪差异不显著(P＞0.05).青、白刺参体壁中均检测出20种主要脂肪酸,脂肪酸总量和多不饱和脂肪酸(PUFA)的含量差异不显著(P＞0.05),而青刺参饱和脂肪酸(SFA)含量显著高于白刺参,单不饱和脂肪酸(MUFA)含量显著低于白刺参(P＜0.05).MUFA中青刺参的花生四烯酸(AA)和二十二碳六烯酸(DHA)含量均显著低于白刺参(P＜0.05).青、白刺参体壁中检测出17种氨基酸,必需氨基酸(EAA)、鲜味氨基酸(FAA)和药效氨基酸(DAA)的含量均无显著差异(P＞0.05).青刺参体壁中天冬氨酸和精氨酸含量均显著低于白刺参(P＜0.05).青刺参体壁中Mn和Cr的含量显著低于白刺参(P＜0.05).重金属元素Pb、Cd、Hg、As的含量均符合相关食品卫生标准.综合评价认为,相同养殖环境条件下,白刺参的出皮率、多糖含量、脂肪酸中不饱和脂肪酸含量、AA和DHA的相对百分含量、氨基酸中天冬氨酸和精氨酸含量以及微量元素中Mn和Cr含量均优于青刺参.     

獐子岛夏秋季野生仿刺参体壁营养成分的分析

对大连獐子岛海域8月和11月的野生仿刺参体壁中的水分、粗蛋白质、灰分、糖、粗脂肪含量以及脂肪酸和氨基酸组成进行了测定.试验结果表明,8月和11月仿刺参体壁中的水分粗蛋白、糖含量(质量分数)分别为89.01%、91.53%;4.65%%、3.25%;0.80%、0.15%,两月间差异极显著(P<0.01);灰分、粗脂肪含量分别为3.02%,2.79%;0.28%,0.33%,两月间无显著性差异.仿刺参体壁中的必需氨基酸含量分别占氨基酸总量的30.08%和20.02%,变异系数为20.18%,说明两月仿刺参体壁中的必需氨基酸含量占氨基酸总量的变化较大;8月和11月仿刺参体壁中廿碳五烯酸(EPA)含量分别为9.94%和11.24%,廿二碳六烯酸(DHA)含量分别为5.83%和7.02%,变异系数分别为8.71%和13.01%,表明8月与11月仿刺参体壁中的EPA、DHA含量有较大变化,11月高于8月.     

蛋氨酸硒含量对食用仿刺参生长和体壁营养成分的影响

在水温10.2~12.4℃、p H 7.8~8.0、盐度24~28条件下,将平均体质量为(45±5)g的仿刺参Apostichopus japonicus饲养在室内养殖池的网箱中,投喂蛋氨酸硒添加量分别为0mg/kg、0.2mg/kg、0.4mg/kg、0.6mg/kg、0.8mg/kg和1.0mg/kg的6组饲料。饲养60d后,测定仿刺参的特定生长率(SGR)和体壁中常规营养成分含量。结果表明:当蛋氨酸硒添加量为1.0mg/kg饲料时,仿刺参的特定生长率、粗蛋白、粗灰分含量最大,较对照组分别高155.56%、5.65%和2.9%(P0.05),而仿粗脂肪含量最低,较对照组低10.61%(P0.05)。     

花生秧部分替代马尾藻对仿刺参生长、消化的影响

利用花生秧粉替代配合饲料中的马尾藻粉,研究不同比例花生秧替代马尾藻的配合饲料对仿刺参幼参的生长和消化的影响。试验设6组,替代比例分别为0％（对照组）、8％、16％、24％、32％和40％,每组3个重复,试验进行60 d。试验结果表明,各组仿刺参存活率均超过95％,且组间差异不显著（P ＞0．05）;24％替代组仿刺参特定生长率最大（0．58％／d）,高于对照组28．9％,8％替代组和16％替代组生长稍慢于对照组,但差异不显著（ P ＞0．05）,而32％替代组和40％替代组仿刺参生长明显慢于对照组（P ＜0．05）;各替代组水体化学需氧量、氨氮和亚硝酸盐氮含量与对照组差异不显著（P ＞0．05）;各替代组幼参对饲料消化率随替代比例变大而降低;试验前30 d肠道内蛋白酶和淀粉酶活性均高于后30 d ,淀粉酶活性对照组表现最大,并有随花生秧粉替代比例变大含量下降的趋势;5个替代组仿刺参体腔超氧化物歧化酶和碱性磷酸酶活力与对照组差异不显著（P ＞0．05）。     

北方刺参与南方糙海参口感及营养成分比较分析

为了解南方和北方主要海参品种的口感及营养品质差异,分别对北方刺参(Apostichopus japonicus)和南方糙海参(Holothuria scabra)体壁的口感和营养成分,包括粗蛋白、灰分、总糖、粗脂肪、氨基酸组成、活性多糖及3种主要常量元素进行了比较分析。结果显示,糙海参的硬度、弹性明显高于刺参,而吞咽度和润滑感低于刺参;干样复水后,刺参的含水量(94.36%)明显高于糙海参(81.79%)。以干基计,刺参和糙海参的粗蛋白和灰分质量分数分别为62.51%、5.62%和81.01%、1.43%;总糖和粗脂肪质量分数分别为8.20%、1.23%和2.15%、1.40%。氨基酸分析显示:糙海参的谷氨酸、甘氨酸和脯氨酸的含量明显高于刺参,尤其胶原蛋白特征氨基酸——甘氨酸、脯氨酸的含量明显高于刺参,因此糙海参含有较多的胶原蛋白;刺参和糙海参的必需氨基酸含量分别为33.06%和18.51%,糙海参明显低于刺参。以干基计,刺参体内矿物元素Ca、Fe和Zn的含量和多糖含量高于糙海参。     

不同剂量的β-胡萝卜素对仿刺参（Apostichopus japonicus）组织和粪便中β-胡萝卜素含量的影响

饲料中分别添加30（C组）、60（B组）、90（A组）mg／kg的β-胡萝卜素。A组仿刺参肠中β-胡萝卜素含量最高（42．67μg／g）,B组次之（12．07μg／g）,对照组第三（11．76μg／g）,C组最低（10.45μg／g）,A组极显著高于其他组（P〈0．010）,其他3组相差不明显（P〉0．050）,可以看出高剂量β-胡萝卜素添加时仿刺参肠中可大量储存β-胡萝卜素。体壁中,B组仿刺参β-胡萝卜素含量最高（1．61μg／g）,A组次之（1．58μg／g）,对照组第三（1．55μg／g）,C组最低（1．54μg,／g）,四组差异均不显著（（P〉0．050）,可知β-胡萝卜素对仿刺参体壁中β-胡萝卜素没有显著性影响。粪便中β-胡萝b素含量：A组〉B组〉C组,D（对照）组,且与饲料中β-胡萝卜素添加量有良好的相关性,说明仿刺参的β-胡萝卜素摄入量与排泄量正相关。     

5种海藻在刺参幼参饲料中的应用研究

本研究通过分析刺参(Apostichopus japonicus)幼参[(10.02±0.03) g]生长性能、体组成、肠道消化酶活性及非特异性免疫性能的变化,评价鼠尾藻(Sargassum thunbergii)、铜藻(Sargassaum horneri)、海带(Saccharina japonica)、海带渣、石莼(Ulva lactuca L.)及混合藻粉在刺参幼参养殖中的应用效果,在室内循环水系统中进行了为期56 d的养殖实验。结果显示,1) 鼠尾藻和混合藻粉组刺参的增重率(WGR)和特定生长率(SGR)极显著高于其他各藻粉组(P<0.01),海带渣组刺参的WGR和SGR最低,肠体比(IBR)和脏体比(VBR)均显著低于其他各组(P<0.05),藻粉对刺参存活率(SR)影响不显著(P>0.05);2) 不同藻粉对刺参体壁水分、粗灰分、粗蛋白和粗脂肪影响不显著(P>0.05);3) 鼠尾藻和混合藻粉组肠道胰蛋白酶活性极显著高于海带、海带渣和石莼组,海带渣组最低(P<0.01);藻粉对α-淀粉酶和脂肪酶活性无显著影响(P>0.05),但对刺参粪便的酸不溶性灰分具有极显著影响(P<0.01);4) 鼠尾藻组和混合藻粉组刺参肠道超氧化物歧化酶(T-SOD)、酸性磷酸酶(ACP)和碱性磷酸酶(AKP)活性极显著高于其他各组(P<0.01),海带渣组丙二醛(MDA)含量极显著高于其他各组(P<0.01)。研究表明,在本实验条件下,综合考虑刺参的生长性能及非特异性免疫性能,添加任一海藻均未达到替代鼠尾藻的饲喂效果,但多种海藻混合添加,其生长性能及非特异性免疫性能与单独添加鼠尾藻效果一致。     

3种不同体色刺参体壁营养成分的比较研究

本研究分别对青色、紫色和白色刺参(Apostichopus japonicus)体壁的营养成分,包括蛋白、脂肪、粗多糖、氨基酸组成、脂肪酸组成及微量元素进行了比较分析。结果显示,青色刺参蛋白含量显著低于紫色和白色刺参(P<0.05),脂肪含量三者无显著差异(P>0.05),青色刺参和紫色刺参的粗多糖含量高于白色刺参(P<0.05)。3种体色刺参检测出17种氨基酸,白色和紫色刺参氨基酸含量相对较高,为48%左右,而青色刺参体壁氨基酸含量为44%左右。紫色和白色刺参的赖氨酸、苯丙氨酸、苏氨酸、缬氨酸等氨基酸的含量均显著高于青色刺参(P<0.05)。亚油酸(LA)、花生四烯酸(AA)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)在3种刺参占较高比重,其中,LA作为重要必需脂肪酸在紫色和白色刺参中均显著高于青色刺参(P<0.05),为青色刺参的4.6倍左右;紫色和白色刺参的AA含量也显著高于青色刺参(P<0.05);而青色刺参中的EPA和DHA均显著高于紫色和白色刺参(P<0.05)。在检测的矿物质中,Fe在3种刺参中含量最高,紫色刺参Fe的含量显著低于青色和白色刺参(P<0.05);青色刺参Mn的含量显著高于紫色和白色刺参(P<0.05);白色刺参Cr的含量显著高于青色和紫色刺参(P<0.05)。研究表明,3种体色刺参的营养成分存在较大差异,均具有进一步开发利用价值。     

Effects of dietary lipid level on growth performance,body composition and digestive enzymes activity of juvenile sea cucumber,Apostichopus japonicus

A feeding trial aimed to determine the effects of dietary lipid level on growth performance, body composition and digestive enzymes activity of juvenile sea cucumber, Apostichopus japonicus. Diets with six crude lipid levels (1.9, 13.8, 29.1, 43.6, 59.6 and 71.6 g kg^?1) were fed to sea cucumbers (initial weights 0.65 ± 0.01 g) at a density of 30 juveniles, once a day. After 60 days, body weight gain (BWG), specific growth rate (SGR), feed intake (FI) and protein efficiency ratio (PER) decreased with increasing dietary lipid levels. The sea cucumbers fed 1.9 g kg^?1 crude lipid showed significantly higher (P < 0.05) BWG than those of the sea cucumbers fed 59.6 and 71.6 g kg^?1 crude lipid. Intestinal protease and lipase activities generally increased with increasing dietary lipid levels. Eicosapentaenoic acid (EPA) content of body walls generally increased with increasing dietary lipid levels. Docosahexaenoic acid (DHA) content of body walls reached the maximum value at a dietary lipid level of 13.8 g kg^?1. N‐3 highly unsaturated fatty acid content followed the same pattern of DHA. According to the growth performance and body composition of sea cucumbers, it can be indicated that the optimum dietary lipid level for juvenile sea cucumbers is between 1.9 and 13.8 g kg^?1.     

Feasibility of Offshore Co‐culture of Abalone,Haliotis discus hannai Ino,and Sea Cucumber,Apostichopus japonicus,in a Temperate Zone

Feasibility of offshore co‐culture of abalone and sea cucumber was investigated in Northern China. Survival and growth of abalone, Haliotis discus hannai Ino, and sea cucumber, Apostichopus japonicus, co‐cultured in abalone cages from suspended longlines, in the offshore area, were examined. Abalone and sea cucumbers were co‐cultured at density ratios of both 3:1 and 6:1 for 1 yr. Abalone were fed with fresh kelp and no additional feed was given to sea cucumbers. Survival of abalone and sea cucumber was 100% for all treatments. Abalone and sea cucumber grew well; the body weight (BW) of abalone and sea cucumber was nearly doubled and had reached a commercial size. There were no significant differences in the growth rates for both abalone and sea cucumber between the two density treatments. The specific growth rate of BW of abalone (SGRbw) was highest in June, with a value of 0.536%/d. Growth rate of sea cucumber (SGRsc) was highest in December, reached 1.84%/d, with an annual average SGRsc of 0.182%/d. Results suggested that the offshore co‐culture of abalone and sea cucumber was feasible offshore. The co‐culture of abalone with sea cucumbers may provide an additional valuable crop without additional financial input.     

几种饲料原料对刺参幼参生长和体成分的影响

研究了不同饲料原料对刺参Apostichopus japonicus(Selenka)幼参生长和体成分的影响.实验采用鱼粉、鼠尾藻Sargassum thunbergii Kuntze、浒苔Enteromorpha prolifera、海带Laminaria japonica和海泥5种主要原料配制的饲料投喂体长为4.18±0.23cm、体重为5.99±0.26g的刺参幼参,进行了为期80d的养殖实验.实验结束时,投喂鼠尾藻饲料、浒苔饲料和动物性饲料的刺参特定生长率(SGR)分别为95.36%/d、92.29%/d和84.87%/d.这三者之间无显著差异,但生长效果要好于投喂海带饲料和海泥饲料的刺参.实验结果表明,在特定的室内养殖条件下,虽然刺参能够更好地利用植物性蛋白,但是可以将动物性蛋白作为刺参的辅助性饲料成分,配制出营养更全面的配合饲料;浒苔作为刺参幼参的养殖饲料原料是完全可行的;海带粗加工产品不适于用做刺参饲料的主要成分.     

Effects of Dietary Lysine Levels on Growth,Intestinal Digestive Enzymes,and Coelomic Fluid Nonspecific Immune Enzymes of Sea Cucumber,Apostichopus japonicus,Juveniles

A 56‐d feeding trial was conducted to evaluate the effects of dietary lysine levels on growth performance, intestinal digestive enzymes, and nonspecific immune response of Apostichopus japonicus juveniles. Five semipurified diets were formulated to contain graded levels of lysine (from 0.28 to 2.32% of dry matter). Although no significant differences (P > 0.05) were observed in survival rate, final weight (FW), and body weight gain (BWG), the FW and BWG increased from 0.28 to 1.19% with increasing dietary lysine levels, then showed a declining tendency. Broken‐line regression analysis of BWG indicated sea cucumber requires lysine at 0.76% of dry diet. Proximate composition, lysine content of the body wall, and total amino acid profiles were not affected by dietary treatments. Intestinal protease level increased with increasing dietary lysine level from 0.28 to 1.19%, then decreased. Acid phosphatase and superoxide dismutase activities of the sea cucumbers were not affected by various dietary lysine levels; however, significantly (P < 0.05) higher alkaline phosphatase activity was observed in sea cucumbers fed 2.32% lysine diet than those fed diets having 0.28 and 1.89% lysine. Overall, sea cucumber juveniles fed with diets containing between 0.76 and 1.19% lysine showed better growth performance, digestive enzymes, and nonspecific immune enzyme activities.     

饥饿胁迫对刺参(Apostichopus japonicus)免疫和生长的影响

为了探究饥饿胁迫对刺参免疫和生长的影响,在11?13℃条件下,研究体质量为(20±0.15) g的刺参在不同时间(0、10、20、30、40、50、60 d),饥饿胁迫对体腔液中酸性磷酸酶(ACP)活性、溶菌酶(LZM)活性、超氧化物歧化酶(SOD)活性、呼吸爆发(RB)活性、吞噬活性以及对刺参体质量、脏壁比、存活率的影响。研究结果表明,随着饥饿时间的延长,刺参体腔液中的ACP活性、LZM活性呈现降低的趋势,饥饿60 d后,比初始值分别下降47.06%、17.57%;SOD活性、RB活性和吞噬活性呈现出先上升后下降的趋势,分别在饥饿胁迫第20、20、10天时达到最高值依次为32.88、0.328、1.35 U/ml,其后显著下降,第60天时显著低于初始值,分别下降27.87%、38.08%、53.43%;其体质量在第60天时为初始体质量的68.08%,呈现负生长;脏壁比逐渐增大,第60天时为0.56,显著高于初始值0.44 (P<0.05)。随着存储营养物质的消耗,刺参体质量损失率增加,存活率下降,存活率与体质量损失率之间存在着y = ?0.0354x2+0.4354x 99.117的函数关系,呈二次曲线线性负相关。研究结果显示,饥饿时刺参通过消耗体内的营养物质来满足机体需要,长期的饥饿有可能降低刺参的免疫能力,直接影响刺参的健康和生长。     

Optimum dietary protein level for growth and coelomic fluid non‐specific immune enzymes of sea cucumber Apostichopus japonicus juvenile

A feeding trial was conducted to determine the optimum dietary protein level of sea cucumber Apostichopus japonicus juvenile focusing on growth performance and non‐specific immune response. Diets with seven crude protein levels (42.0, 108.9, 155.2, 216.7, 258.0, 313.3 and 357.5 g kg^?1) were fed to sea cucumber juveniles (1.05 ±0.01 g) once a day for 100 days. More than 70% survival was observed, and there was no significant difference among all treatments. The sea cucumbers fed diets containing 108.9 g kg^?1 crude protein showed significantly (P < 0.05) higher body weight gain than those of the sea cucumbers fed diets containing 42.0, 216.7, 258.0, 313.3 and 357.5 g kg^?1 crude protein. No significantly differences (P > 0.05) were observed in moisture, crude protein, crude lipid, ash and carbohydrate content of the body wall among all treatments. The coelomic fluid catalase activity of the sea cucumbers generally increased with increasing dietary protein levels. Therefore, the acid phosphatase, superoxide dismutase and lysozyme activity increased with increasing dietary protein levels at first and decreased subsequently. The relationship between dietary protein levels and body weight gain was analysed by a second‐order polynomial regression analysis model. The result indicates that the optimum dietary protein level for sea cucumber juveniles is 135.4 g kg^?1.     

Optimum Level of Dietary n‐3 Highly Unsaturated Fatty Acids for Juvenile Sea Cucumber,Apostichopus japonicus

A feeding trial was conducted to estimate the optimum level of dietary n‐3 highly unsaturated fatty acids (HUFAs) for juvenile sea cucumber, Apostichopus japonicas, based on growth performance and fatty acid compositions. Diets with five n‐3 HUFAs levels (0.15, 0.22, 0.33, 0.38, and 0.46%) were fed to sea cucumber juveniles (1.97 ± 0.01 g) once a day for 60 d. The sea cucumbers fed diets containing 0.22% n‐3 HUFAs showed significantly (P < 0.05) higher body weight gain, feed efficiency, and protein efficiency ratio than the sea cucumbers fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.33, 0.38, and 0.46% n‐3 HUFAs. The sea cucumbers fed diets containing 0.46% n‐3 HUFAs showed significantly (P < 0.05) higher eicosapentaenoic acid and saturated fatty acid than the sea cucumber fed diets containing 0.15% n‐3 HUFAs, but not significantly different (P > 0.05) from those of sea cucumbers fed diets containing 0.22, 0.33, and 0.38% n‐3 HUFAs. The results of growth performance and n‐3 HUFA compositions of body wall indicated that the optimum level of dietary n‐3 HUFAs for juvenile sea cucumber is between 0.22 and 0.46%.