中华鳖裙边胶原蛋白的提取及其特征

胶原蛋白是生物体内一种重要的蛋白质,也是结缔组织的主要蛋白成分。与陆生动物(如猪、牛等)比较,水生动物胶原蛋白具有优良的理化性质和生理活性功能,已引起人们的广泛关注。中华鳖是我国传统的名贵水产品,其裙边胶原蛋白含量丰富,具有极高的药用价值和营养价值。采用胃蛋白酶酶解法在酸性条件下提取中华鳖裙边胶原蛋白,研究酶添加量、底物浓度、提取时间对提取效果的影响,确定了制备裙边胶原蛋白的最适条件为：酶添加量4 mg/mL,底物浓度4%,提取时间14 h。粗提胶原蛋白通过盐析、透析等纯化步骤,再经SDS-PAGE电泳、热变性温度测定、紫外及傅立叶变换红外扫描等技术方法分析了胶原蛋白的生化特征,结果显示提取到的裙边胶原蛋白具有较高的纯度,含有两条α链和1条β链,属于典型的I型胶原蛋白。研究表明：中华鳖裙边胶原蛋白可以作为猪皮、牛皮胶原蛋白的有效替代品,这为今后进一步综合开发利用鳖类裙边胶原多肽提供了科学的理论依据。     

蝇蛆蛋白对中华鳖生长性能及营养品质的影响

在室内、外养殖试验中研究了将家蝇蛆磨成浆按一定比例配以卵磷脂、几丁寡糖和多种维生素制成的蝇蛆蛋白对中华鳖生长性能及营养品质的影响。在水温(25±1)℃的室内养殖试验中,将36只体质量(248.15±17.10)g的中华鳖,随机分成3组(重复3次,每重复4只)饲养在120cm×60cm×55cm水族箱中,分别投喂基础饲料(对照组)、15%蝇蛆蛋白+基础饲料和30%蝇蛆蛋白+基础饲料,养殖期180d。室外养殖池面积为5000 m~2,中华鳖体质量(294.05±12.265)g,每口池塘放雄鳖7200只,投喂基础饲料(对照组)和15%蝇蛆蛋白+基础饲料,养殖期120d。结果表明,在饲料中添加15%蝇蛆蛋白显著提高了中华鳖的质量增加率与特定生长率,降低了饲料系数。当添加15%蝇蛆蛋白时,中华鳖肌肉与裙边中蛋白质、必需氨基酸、呈味氨基酸、单不饱和脂肪、多不饱和脂肪酸的含量均显著高于对照组,而饱和脂肪酸则显著低于对照组。用蝇蛆蛋白养殖的中华鳖肌肉和裙边中必需氨基酸占总氨基酸的比值、呈味氨基酸占总氨基酸的比值及二十碳五烯酸与二十二碳六烯酸的总含量也均显著高于对照组。以上结果表明,在饲料中添加15%蝇蛆蛋白能促进中华鳖生长,降低饲料系数,提高中华鳖的营养价值与药用保健功能。     

中华绒螯蟹池塘套养与温室养殖的中华鳖雄体生物学指数和营养成分比较

分别取中华绒螯蟹(Eriocheir sinensis)池塘套养和温室养殖中华鳖(Trionyx sinensis)雄体(分别简称为"套养鳖"和"温室鳖")各5只,解剖取四肢肌肉、裙边和肝脏,分析和比较两种鳖的生物学指数,可食组织常规营养成分、氨基酸含量和脂肪酸组成的差异。结果显示:(1)温室鳖裙边指数(SI)和肝体比(HSI)均显著高于套养鳖(P0.05)。(2)套养鳖肌肉灰分和裙边水分含量均显著高于温室鳖,而裙边蛋白含量则以温室鳖较高(P0.01),套养鳖和温室鳖其余常规营养成分均无显著差异(P0.05)。(3)温室鳖肌肉和裙边中的大部分氨基酸、总必需氨基酸(∑EAA)和总氨基酸(TAA)含量显著高于套养鳖,半胱氨酸含量及EAA/TAA以套养鳖较高(P0.05);套养鳖和温室鳖肌肉和裙边中的必需氨基酸评分(EAAS)较为接近,而EAAS平均值以套养鳖较高。(4)温室鳖肌肉C18:1n9、C18:1n7及单不饱和脂肪酸(∑MUFA)总量显著高于套养鳖,而C22:6n3、总多不饱和脂肪酸(∑PUFA)、∑n-3PUFA、总高度不饱和脂肪酸(∑HUFA)含量及n-3/n-6以套养鳖较高(P0.05);就裙边而言,除温室鳖C17:0和C20:2n6含量显著高于套养鳖外(P0.05),其余脂肪酸含量均无显著差异(P0.05)。综上可见,中华鳖在两种养殖模式下均具有较高的营养价值。     

甜菜碱对淡水白鲳的转甲基功效及蛋白质合成的影响

以淡水白鲳(Colossoma brachypomum)鱼种为试验对象,养殖试验周期为60 d。平均试验水温(26.5±0.5)℃。基础饲料粗蛋白水平(质量分数)为41.03％,添加盐酸甜菜碱水平(质量分数)分别为0、0.1％、0.2％、0.4％、O.6％和0.8％。试验结束后,停饲24h取样测定。结果表明,盐酸甜菜碱使整鱼水分含量呈下降趋势(P>0.05),粗蛋白含量呈上升趋势,其中,0.6％甜菜碱组整鱼粗蛋白提高11.89％(P<0.05);鱼肌肉含水量下降,0.1％、O.6％和O.8％甜菜碱组分别降低1.29％(P<0.05)、1.42％(P<0.05)和2.45％(P<0.01),肌肉粗蛋白含量提高,0.4％甜菜碱组提高3.19％(P<0.05);肝脏粗蛋白含量提高,0.2％、0.4％、0.6％和0.8％组分别提高1.04％、O.74％、0.75％和0.87％(4组P<0.01);盐酸甜菜碱显著提高肌肉和肝脏中RNA和TNA含量及RNA与DNA比率;盐酸甜菜碱显著提高肝脏中甜菜碱-高半胱氨酸甲基转移酶和胱硫醚β-合成酶总活力和比活力。本试验结果提示,甜菜碱在肝脏BHMT和β-CYST的双重调控下作为甲基供体参与机体代谢活动,使肝脏和肌肉中甲基化产物(如:核酸)含量增加,从而实现其促进蛋白质合成的功能。     

蟹塘套养与温室养殖的中华鳖雌体营养组成的比较研究

实验研究了中华绒螯蟹(Eriocheir sinensis)池塘套养与温室养殖的中华鳖(Trionyx sinensis)雌体(分别简称"套养鳖"和"温室鳖")的生物学指数,肌肉和裙边中的常规营养成分、氨基酸含量和脂肪酸组成。结果显示:(1)套养鳖裙边指数略高于温室鳖,而套养鳖肝体指数显著低于温室鳖。(2)套养鳖肌肉和裙边中的水分含量显著高于温室鳖,而两种组织中的粗蛋白含量均以温室鳖较高。(3)套养鳖肌肉和裙边中的总必需氨基酸、总非必需氨基酸和总氨基酸含量均显著低于温室鳖;除色氨酸外,温室鳖肌肉中的大部分必需氨基酸分(EAAS)高于套养鳖,而裙边中的所有EAAS均以套养鳖较高。(4)套养鳖肌肉中的C16∶0、C18∶2n6、C18∶3n6、C20∶2n6和C20∶3n3含量显著低于温室鳖,而其C16∶1、C18∶3n3、C20∶3n6和C20∶4n6含量较高;套养鳖裙边中的C18∶3n6、C20∶2n6、C20∶3n6、C20∶4n6含量显著高于温室鳖,而C18∶1n9、C18∶2n6、C18∶3n3、总n-3多不饱和脂肪酸和n-3/n-6多不饱和脂肪酸之比均以温室鳖较高。结果表明,温室鳖肌肉和裙边的干物质含量较高,尤其是蛋白质含量,而套养鳖的氨基酸和脂肪酸组成更优。     

大菱鲆不同部位营养与质构品质分析评价

以大菱鲆(Scophthatmus maximus)为研究对象,系统比较分析了其背部、腹部、胸腔部、尾部、裙边和鱼皮的基本营养成分、胶原蛋白含量、氨基酸与脂肪酸组成及质构特性,并进行了营养价值评价,获得了大菱鲆各部位营养与质构品质的基础数据。研究结果显示,不同部位的粗蛋白含量存在差异,鱼皮中含量最高为29.04%,而裙边含量最低为12.99%,与其他部位肌肉差异均极显著(P<0.01)。背部、腹部、胸腔部和尾部的粗蛋白含量分别为18.76%、18.96%、17.91%和18.39%;裙边粗脂肪含量最高达17.47%,脂肪酸种类最多为27种,并且含量达318.09 mg/g,多不饱和脂肪酸的含量为其他部位的3.90~6.76倍,其中亚油酸含量最高(107.26 mg/g),其次为DHA(64.39 mg/g)和EPA(26.61 mg/g);鱼皮中胶原蛋白含量达224.69 mg/g,显著高于其余部位(P<0.01),可作为制备胶原蛋白的原料。各部位中均检测出18种氨基酸,背部、腹部、胸腔部、尾部和裙边中第一限制性氨基酸均为Met+Cys,而在鱼皮中第一限制性氨基酸为Trp;另外,大菱鲆除鱼皮之外,其余各部位氨基酸组成均符合FAO/WHO参考模式标准;通过质构特性分析,发现胸腔部肌肉的硬度、咀嚼性和弹性显著高于其余部位(P<0.05),口感更佳。     

中华鳖营养、食用开发现状及湖南地区产业优势

中华鳖(Pelodiscus sinensis)是我国特色水产养殖品种,因具独特的营养价值和膳食功效,自古以来是食疗滋补上品。本文综述了中华鳖滋补功效及其机理研究进展和食用开发的现状,以及湖南地区养殖优势。首先阐述中华鳖富含多糖、胶原蛋白、不饱和脂肪酸等多种营养物质,不仅可以作为高蛋白低脂肪的滋补原料,而且在肿瘤相关疾病防治、免疫力提高与延缓衰老等方面食用功效显著。     

草鱼(Ctenopharyngodon idellus)、梭鱼(Liza haematocheila)、黑石斑鱼(Centropristis striata)的营养成分及加工品质比较

本研究对不同养殖环境和食性的草鱼(Ctenopharyngodon idellus)、梭鱼(Liza haematocheila)、黑石斑鱼(Centropristis striata)的营养成分和加工品质进行了分析比较。对鱼体的解剖测量结果显示,梭鱼头重占体重的比例为17.09%,低于黑石斑鱼和草鱼。对3种鱼的胶原蛋白和氨基酸成分分析结果显示,黑石斑鱼肌肉可溶性与不可溶性胶原蛋白含量均为最高,分别为0.22 mg/ml和2.12 mg/ml,梭鱼含量次之,分别为0.05 mg/ml和0.82 mg/ml。黑石斑鱼的鲜味氨基酸含量最高(44.37%),梭鱼为32.80%,均显著高于草鱼(24.50%)。6种必需氨基酸总含量:草鱼最低,为53.31%,梭鱼为57.14%,黑石斑鱼最高,为62.64%,三者之间差异显著。肌肉脂肪酸检测显示,梭鱼饱和脂肪酸(SFA)含量最高,为41.26%(P0.05);黑石斑鱼多不饱和脂肪酸含量(PUFA)最高,为34.58%(P0.05)。梭鱼二十碳五烯酸(EPA)的含量为8.27%,与黑石斑鱼(7.85%)无显著差异。黑石斑鱼的二十二碳六烯酸(DHA)含量最高,为13.51%,显著高于草鱼(3.84%)和梭鱼(3.02%)。3种鱼肌肉中超氧化物歧化酶(SOD)活力有显著差异,梭鱼最强,黑石斑鱼最弱。丙二醛(MDA)含量检测结果显示,黑石斑鱼的MDA含量为19.98 nmol/mg,显著高于草鱼和梭鱼,草鱼和梭鱼差异不显著,可见梭鱼肌肉抗氧化能力较强,有利于进行长期保存、加工。     

饲料蛋白质和脂肪水平对中华鳖生长性能、肌肉质构指标及肝脏相关基因表达的影响

为研究饲料蛋白质和脂肪水平对中华鳖(Pelodiscus sinensis)的生长性能、肌肉质构指标及肝脏相关基因表达的影响,实验采用双因素3×2因子试验设计,制成蛋白质水平分别为40%、45%和50%,脂肪水平分别为6%和9%的6组试验饲料,饲喂均重为(293.11±7.84)g的中华鳖,8周后测定相关指标。结果显示:饲料蛋白质和脂肪水平对中华鳖存活率无显著影响,但对增重率、特定生长率、饲料系数和肝体比均有显著影响。随着饲料蛋白质水平的升高,增重率和特定生长率均显著升高,45%和50%蛋白组增重率和特定生长率显著高于40%组,45%组饲料系数显著低于40%组,50%组的肝体比显著高于40%组。饲料脂肪含量对中华鳖生长性能无显著影响,9%脂肪组的肝体比显著高于6%组。饲料蛋白质和脂肪水平对肌肉水分和粗灰分含量无显著影响,肌肉蛋白质含量随着饲料蛋白质水平的增加先显著升高,然后在45%时达到稳定水平。肌肉脂肪含量随着饲料脂肪水平增加显著升高。饲料蛋白质和脂肪水平对中华鳖四肢肌肉的回复性、弹性和凝聚性无显著影响,硬度、黏性和咀嚼性随着饲料蛋白质水平升高而显著升高,随着脂肪水平升高而显著下降。肝脏igf-I基因mRNA表达水平随着饲料蛋白质水平的升高而显著升高,9%脂肪组的肝脏igf-I和lpl基因mRNA表达水平显著高于6%组。综合中华鳖生长性能及营养品质的试验结果,温室养殖条件下,中华鳖饲料适宜的蛋白质和脂肪水平分别为45%和6%。     

桑叶黄酮对吉富罗非鱼肌肉抗氧化指标及营养组成的影响

为研究饲料中添加桑叶黄酮对吉富罗非鱼(Oreochromis niloticus)肌肉抗氧化指标及营养组成的影响,选取初始体重为(1.51±0.02)g的吉富罗非鱼840尾,随机分为6组,每组4个重复,每个重复35尾鱼,在56 d饲养期中分别投喂添加桑叶黄酮0、50、100、300、500和1 000 mg/kg的试验饲料。结果显示:100、300 mg/kg组肌肉超氧化物歧化酶(SOD)活性显著高于其他各组,且分别比对照组提高了6.2%和8.7%;500 mg/kg组肌肉总抗氧化力(T-AOC)显著高于1 000 mg/kg组及对照组;50、100、300、1 000 mg/kg组肌肉丙二醛(MDA)含量显著低于对照组。饲料中添加桑叶黄酮对吉富罗非鱼肌肉粗蛋白、粗脂肪、灰分和水分含量没有显著影响,对肌肉滴水损失影响不显著。500 mg/kg组胶原蛋白含量显著高于对照组,与其他组差异不显著。50 mg/kg组肌肉苯丙氨酸含量与对照组相比显著升高。100 mg/kg组肌肉丙氨酸含量显著高于对照组和50、300、500 mg/kg组。300 mg/kg组肌肉亮氨酸、蛋氨酸和谷氨酸和1 000 mg/kg组肌肉组氨酸含量显著高于对照组。结果表明,添加适量的桑叶黄酮可以显著提高吉富罗非鱼肌肉抗氧化指标和胶原蛋白含量,改善肌肉氨基酸组成。综合肌肉抗氧化性能、胶原蛋白含量和氨基酸组成的指标来评价,桑叶黄酮添加水平在300~500 mg/kg时效果较好。     

Effects of dietary zinc levels on growth,serum zinc,haematological parameters and tissue trace elements of soft‐shelled turtles,Pelodiscus sinensis

A 10‐week feeding trial was conducted to evaluate the effects of dietary zinc (Zn) contents on the growth, tissue trace element contents and serum Zn levels in soft‐shelled turtles, Pelodiscus sinensis. Juvenile soft‐shelled turtles approximately 4.8 g in body weight were fed casein‐based diets containing seven levels of Zn (14, 23, 32, 43, 58, 87 and 100 mg kg^?1) for 10 weeks. There were no significant differences (P > 0.05) in weight gain (WG), feed conversion ratio (FCR) or protein efficiency ratio (PER) among the dietary treatments. However, Zn concentrations in the liver, serum and carapace of turtles fed the basal diet containing 14 mg Zn kg^?1 were the lowest among all groups. Zn contents in the liver, serum and carapace increased when dietary Zn increased up to a dietary Zn level of approximately 43 mg kg^?1. Beyond this dietary level, tissue Zn contents were relatively constant. Carapace iron (Fe), selenium (Se) in hard tissues and haemoglobin concentrations decreased when dietary Zn increased. Dietary Zn requirements of juvenile soft‐shelled turtles derived from regression modelling using the liver, serum, carapace and bone Zn contents as indicators were 42, 39, 35 and 46 mg Zn kg^?1, respectively.     

Effects of dietary magnesium on the growth,carapace strength and tissue magnesium concentrations of soft‐shelled turtle,Pelodiscus sinensis (Wiegmann)

A feeding trial was conducted to evaluate the effects of dietary magnesium on the growth, carapace strength, tissue and serum Mg concentration of soft‐shelled turtles, Pelodiscus sinensis (Wiegmann). Juvenile soft‐shelled turtles of approximate 5.4 g body weight were fed diets with seven levels of Mg (48, 206, 369, 670, 955, 1195 and 1500 mg Mg kg^?1) for eight weeks. No significant difference (P ≥ 0.05) was found in weight gain (WG), feed conversion ratio or protein efficiency ratio among treatments. However, the WG of turtles continued to increase with increasing dietary Mg levels up to 670 mg kg^?1, beyond which the WG levelled off. The plasma alkaline phosphatase activity and the muscle, bone Mg concentrations of the turtles increased with the increasing dietary Mg levels between 48 and 955 mg kg^?1, beyond which the tissue Mg concentrations remained relatively constant. Furthermore, the carapace strengths of turtles fed with the control diet of 48 mg Mg kg^?1 were significantly weaker (P < 0.05) than that of turtles fed with diets containing higher Mg levels. Based on a broken‐line modelling analysis, the required dietary Mg level for the optimal WG of juvenile soft‐shelled turtles was estimated to be approximately 650 mg kg^?1. By contrast, the required dietary Mg levels for turtles to reach the optimal muscle and bone Mg concentrations were 1050 and 1000 mg kg^?1 respectively. The required dietary Mg level for maximal alkaline phosphatase activity was approximately 980 mg kg^?1.     

Fillet texture and muscle structure in brown trout (Salmo trutta) subjected to long-term exercise

Brown trout (Salmo trutta) (2.7 g initial mean weight) were reared in freshwater for 8 months at water velocities of <0.1 (control group), 1 or 2 body lengths per second (BL s^?1) (exercise groups). Growth (body weight, body length and body width), muscle structure (muscle fibre diameter and width of myosepta) and flesh quality parameters (dry matter, muscle pH, collagen content and solubility, instrumental evaluation of texture) were measured at the end of the experiment. The body weight of fish at 1 BL s^?1 was 22% higher than the control group. Muscle development was stimulated at 1 and 2 BL s^?1, leading to a higher condition factor, greater body height and width, and muscle fibre hypertrophy (55 vs. 59.5 μm fibre diameter in the control and 2 BL s^?1 groups respectively). Connective tissue and collagen were only slightly affected by exercise (no difference in collagen solubility, but a greater proportion of γ trimer and fewer α chains in the control compared with the exercised group). Flesh quality was affected, with greater dry matter content and lower post‐mortem pH in the 1 BL s^?1 group compared with the control and 2 BL s^?1 groups. The mechanical resistance of the raw fillets was slightly but significantly increased by exercise. The exercise‐related changes in muscle structure and texture are discussed.     

Estimation of dietary vitamin K requirement of juvenile Chinese soft‐shelled turtle,Pelodiscus sinensis

A 10‐week feeding trial was conducted to estimate the dietary vitamin K requirements of juvenile Chinese soft‐shelled turtles, Pelodiscus sinensis, using menadione sodium bisulphite (MSB) as the vitamin K source. Juvenile soft‐shelled turtles with 6.1 ± 0.1 g body weight were individually reared and fed diets containing seven levels of MSB (0.0, 5.0, 8.9, 12.3, 16.6, 20.7 and 41.0 mg/kg). Turtles fed with an MSB‐free diet exhibited the lowest feed utilization, carapace Ca concentrations, carapace strength or liver menaquinone (MK‐4) concentrations among all dietary groups. Weight gain (WG) in the turtles tended to increase with increase in dietary MSB until a dietary MSB level of 16.6 mg/kg, where WG levelled off. Total plasma prothrombin concentrations, an indicator for blood clotting function, in the turtles fed on MSB supplemented diets were significantly (p < 0.05) lower than those in turtles fed on MSB supplemented diets. Liver MSB concentrations increased with increase in dietary MSB. Using broken‐line or sigmoidal regression model, the vitamin K requirements of juvenile Chinese soft‐shelled turtles based on WG, total plasma prothrombin concentrations and liver vitamin K were 21.5, 25.8 and 29.9 mg MSB per kg, respectively.     

Effects of dietary betaine in allogynogenetic gibel carp (Carassius auratus gibelio): Enhanced growth,reduced lipid deposition and depressed lipogenic gene expression

A 10‐week feeding experiment was conducted to evaluate the effects of supplemental betaine on growth performance, body composition and lipid deposition in allogynogenetic gibel carp. Four isoproteic (37% crude protein) and isolipidic (5% crude lipid) artificial diets with 0%, 0.08%, 0.4% and 2% betaine supplementation were formulated, and named VB₀, VB_0.08, VB_0.4 and VB₂, respectively. Each diet was fed in triplicate to fish about 10 g in weight. The results showed that 0.4% betaine supplementation significantly improved growth performance and reduced lipid content in the hepatopancreas, muscle and the whole body compared with the control group. Moreover, both fatty acid synthase and acetyl‐CoA carboxylase, two important lipogenic genes, showed significantly lower expression in the VB_0.4 group than in the control group, and a strong correlation was detected between lipid content and mRNA expression levels for FAS and ACC in the hepatopancreas. Taken together, appropriate (0.4%) betaine supplementation in the diet not only improved growth, but also reduced lipid deposition in allogynogenetic gibel carp, probably by diminishing lipogenic gene expression.     

杜仲对凡纳滨对虾生长、血清非特异性免疫和肌肉成分的影响

在基础饲料中分别添加0.0%(对照组)、0.5%、1.0%、2.0%和3.0%的杜仲(Eucommia ulmoides), 饲喂体质量为(7.5±0.2) g的凡纳滨对虾(Litopenaeus vannamei), 实验共分5个处理组, 每处理组4个重复, 每重复40尾虾。经过42 d养殖, 各处理组均有较高的存活率, 且无显著差异(P>0.05); 2.0%杜仲组的虾体增重率最高(136.1%), 饲料系数最低(1.33), 较对照组提高增重率9.8%(P<0.05), 降低饲料系数0.13(P<0.05); 饲料中添加0.5%、1.0%杜仲, 显著提高了对虾血清LSZ、PO活性, 添加1.0%杜仲, 显著降低了血清MDA含量, 提高了肝胰腺蛋白酶活性(P<0.05); 攻毒实验结果表明, 以溶藻弧菌(Vibrio alginolyticus)肌肉注射虾体后96 h, 0.5%、1.0%、2.0%杜仲组的虾体死亡率均较对照组显著降低(P<0.05); 在肌肉成分方面, 添加2.0%、3.0%杜仲显著提高了肌肉胶原蛋白含量, 各处理在肌肉水分、灰分、粗蛋白、粗脂肪含量方面没有显著差异。上述研究表明, 在凡纳滨对虾饲料中添加杜仲2.0%, 可显著改善生产性能, 提高肌肉胶原蛋白含量; 在饲料中添加杜仲0.5%～1.0%, 可提高凡纳滨对虾非特异性免疫能力。本研究旨在考察杜仲对凡纳滨对虾生长、血清非特异性免疫和肌肉成分的影响, 为杜仲在对虾饲料中的合理应用提供科学依据。

     

脂质对中华花龟不同组织脂肪酸组成的影响

将体质量(1828±468)g的4龄雌性成体中华花龟随机分成4组,常温饲养在150cm×50cm×50cm的水池中,每组6只,分别投喂饲料中鱼油为豆油替代0%、33.3%、66.7%和100%的饲料,每周投喂2次。饲养1年后,从每组中随机取6只中华花龟,检测肝脏、肌肉、卵巢中脂肪酸的组成。试验结果表明,饲料中鱼油比例较高(豆油替代0%和33.3%鱼油组)能促进肝脏和肌肉中二十碳五烯酸和二十二碳六烯酸的积累,但豆油替代率对卵巢二十碳五烯酸和二十二碳六烯酸的积累影响不显著。饲料中花生四烯酸在肌肉和卵巢中大量累积,豆油替代33.3%鱼油组肌肉和豆油替代0%鱼油组卵巢中花生四烯酸占总脂肪酸的含量[(5.26±0.94)%、(1.31±0.04%)]最高,肝脏中花生四烯酸积累较少。豆油替代66.7%和100%鱼油组肝脏中亚油酸含量显著高于其他两组(P0.05),豆油替代100%鱼油组卵巢中亚麻酸含量最高(P0.05)。饲料中豆油替代鱼油比例升高促进了中华花龟组织中亚油酸和亚麻酸的积累,抑制了二十碳五烯酸、二十二碳六烯酸和花生四烯酸等的积累;当豆油替代33.3%鱼油时,中华花龟肝脏、肌肉、卵巢中脂肪酸组成及含量与全鱼油组差异不显著。