银杏果浸泡法去皮的贮藏效果及贮藏生理研究

实验以海洋皇马铃型银杏种子(Ginkgo biloba L)为研究对象,研究了饱和石灰水浸泡法、乙烯利浸泡法、焦亚硫酸钠浸泡法、施保功浸泡法及自然浸泡法等常见的银杏种子浸泡去皮(外种皮)方法对银杏种子贮藏效果及贮藏生理的影响对贮藏期间银杏种子呼吸强度、细胞膜透性、α-淀粉酶活性、β-淀粉酶活性、浮水率、硬化率、霉变率及失重率等指标进行了测定.结果表明焦亚硫酸钠浸泡法为合适的银杏浸泡去皮方法.     

采前激素处理对银杏种子的脱皮与保鲜效果的研究

研究了乙烯利20、100、500 mg/L；891植物促长素20、100、500 mg/L；脱落酸20、100、500 mg/L等9种不同采前激素催熟处理方法对银杏种子采后脱皮及贮藏保鲜的影响。其中，乙烯利500 mg/L处理，采后4 d呼吸高峰到来，第6 d果肉软化，容易去皮，且贮藏90 d，除霉变率稍高外，浮水率、失水率、硬化率都较低，还原糖、蛋白质、脂肪、淀粉、蛋白质含量保持较高水平，适宜于以食用为目的的贮藏。891植物促长素500 mg/L处理发芽率高，β-淀粉酶、过氧化氢酶(CAT)、脂肪酶、多     

哈密瓜常温保鲜贮运技术研究

该研究旨在解决新疆哈密瓜采后低温贮运费用高,常温贮运腐烂率高,损失严重的问题。针对哈密瓜采后腐烂的主要病原菌匍枝根霉(R.stolonifer)、链格孢霉(A.Alternata)、半裸镰刀菌(F.semitectum)、脂状青霉(P.digitatum),筛选出对症的抑菌剂,并研究其最适使用浓度;以常温贮藏哈密的瓜失重率、呼吸强度、过氧化氢酶(CAT)、纤维素酶、果胶甲酯酶(PE)、多聚半乳糖醛酸酶(PG)活性等为指标,研究云南虫胶作为被膜对哈密瓜采后生理的影响及其最适使用浓度。含有0.60g/L扑海因、0.8g/L氯硝胺、0.6g/L特克多、0.8g/L施宝功和1∶24(V.V-1)虫胶的混合溶液浸泡哈密瓜10s,于28℃,85%RH,贮藏30d,腐烂率1.87%,同期对照腐烂率49.54%。处理后的哈密瓜在室外温度28～34℃、棚车内温度30～38℃的条件下,经过12～15d由新疆哈密运抵苏州、上海、广州的腐烂率分别为3.37%、4.12%和4.86%。哈密瓜表皮以内0.8cm几乎无抑菌剂残留。用上述配方的虫胶与抑菌剂的混合溶液浸泡哈密瓜,可有效降低哈密瓜采后常温贮运中的腐烂率,保持良好风味和商品质量,大量节省运输费用     

锥栗贮藏过程维生素C的降解速率以及保鲜条件研究

采用试验研究了锥栗贮藏过程中维生素C(维生素C)的降解速率及其保鲜条件。将3个不同品种的锥栗经不同浓度的抑霉唑浸泡，并在3个不同温度(-1～0℃、5℃、10℃)中进行贮藏，测定贮藏中锥栗维生素C的含量的变化。锥栗中维生素C的降解速率与维生素C的浓度成正比，求得降解速率系数k，对其进行非线性拟合，并用极差分析法和方差分析法进行分析研究。试验结果表明，最优的保鲜条件为：温度-1～0℃、品种为长芒、抑霉唑浓度2000 μL/L。     

银杏种子辐照效应及其保鲜的初步研究

不同剂量^60Coγ射线处理去外种皮的银杏种子,50Gy以上剂量处理的种子不能发芽,种胚活力迅速下降而趋于死亡,800Gy以下剂量处理后,胚乳仍保持较长时期的活力。2000Gy以上剂量使种子失水加快,胚乳失活且变软腐烂,共电导率迅速上升,辐照处理使银杏降种子的呼吸受到较大抑制,有利于贮藏保鲜,银杏种子密封包装后,经50－200Gy辐照工在室内常温存放,含水量40．25％的银杏种子的保质期为2－3个月,当种子含水量降至35．96％时,保持期可达4－6个月。     

壳聚糖和抗坏血酸复合处理提高台湾青枣采后保鲜效果

为了延长台湾青枣(Ziziphus mauritiana Lam.)的采后保鲜时间,基于抗坏血酸(ascorbic acid,As A)可提高果实抗氧化能力,壳聚糖涂膜处理能防止果实失水和微生物侵染,该文探讨抗坏血酸、壳聚糖涂膜及抗坏血酸和壳聚糖复合处理对台湾青枣(Ziziphus mauritiana Lam.)采后保鲜效果的影响.果实采收后当天,分别用30.0mmol/L抗坏血酸浸泡处理、8g/L壳聚糖涂膜以及30.0mmol/L抗坏血酸浸泡后并用8g/L壳聚糖涂膜复合处理,以双蒸水浸泡处理为对照,处理结束后,置于(25±1)℃和90%~95%相对湿度下贮藏,定期测定果实相关生理参数,扫描电镜观察果皮果实组织结构.结果表明,与对照相比,抗坏血酸或壳聚糖单独处理虽然对台湾青枣保鲜有一定效果,但复合处理后保鲜效果更好.表现为显著减少果实失水率和相对电导率(P<0.05),抑制果皮叶绿素降解及果实果胶酶和多聚半乳糖醛酸酶活性增加,降低原果胶分解成可溶性果胶的速率(P<0.05),使果实硬度和细胞完整性得以维持;延缓果实超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性下降,降低膜脂氧化速率,果实过氧化氢和膜脂过氧化产物丙二醛含量显著下降(P<0.05);维持果实较高的可溶性固形物(total soluble solids,SS)、可溶性总糖(total soluble sugar,TSS)、可滴定酸(titratable acidity,TA)、谷胱甘肽(glutathione,GSH)及抗坏血酸含量.研究结果说明,抗坏血酸和壳聚糖复合处理可提高台湾青枣贮藏过程中的抗氧化能力,降低氧化伤害;降低果实失水率,减缓果皮叶绿素降解速率,延缓细胞降解和软化速率,维持果实细胞完整性,降低果实的腐烂率,从而达到延长采后果实保鲜的效果.     

不同处理对柴胡种子萌发的影响

对柴胡种子进行自来水浸种、沙藏处理及6-BA、赤霉素、H2O₂和KMnO4药剂处理试验,以研究不同处理对柴胡种子发芽率的影响。结果表明,柴胡种子经自来水浸泡,随浸泡时间的延长,发芽率升高。H2O₂、6-BA、沙藏对柴胡种子发芽有抑制作用,适宜浓度的KMnO4和赤霉素对柴胡种子发芽有促进作用。加热100 ℃ 60 mg/L赤霉素浸泡24 h的柴胡种子发芽率高于其他处理,发芽率达32.00%;用自来水浸泡48 h次之,发芽率为30.00%;用6-BA处理过的柴胡种子未见发芽。     

外源ABA对冬油菜种子萌发及生理指标的影响

用不同浓度的脱落酸(ABA)溶液浸泡白菜型冬油菜陇油6号种子后进行发芽试验,统计种子萌发情况,分析幼苗相关生理指标的变化。结果表明,较低浓度(10～70 mg/L)的脱落酸溶液处理有利于种子的萌发,有效提高种子发芽率、发芽势及发芽指数,提高丙二醛和脯氨酸含量,增强幼苗中过氧化物酶(POD)活性,超氧化物歧化酶(SOD)、过氧化氢酶(CAT),减缓叶绿素含量的下降,其中以30 mg/L脱落酸效果最佳。较高浓度的脱落酸溶液抑制冬油菜种子的萌发。     

红梗叶甜菜种子发芽特性的研究

研究了不同处理条件下红梗叶甜菜种子的发芽特性。结果表明，用一定浓度的双氧水、硼酸、磷酸二氢钾浸种处理红梗叶甜菜种子，可提高种子的发芽率、发芽势和发芽指数，其中以浓度10g／L H2O2、0．5g／L H3BO3和5g／L KH2PO4处理较为适宜；赤霉素处理种子的发芽效果不明显；吸湿一回干处理对红梗叶甜菜种子发芽有良好的促进作用；红梗叶甜菜种球色泽、粒径大小与发芽的关系密切，黄色或黄褐色种球的发芽力显著高于黑色或褐色种球的，大、中粒种子的发芽力显著高于小粒种子的；叶甜菜种子不耐贮藏，发芽力下降较快，使用年限仅为1a。     

多效唑对花生种子萌发及植株生长的影响

以不同浓度的多效唑，对花生种子进行浸种处理和盛花期叶面喷施，观测种子萌发、幼苗生长情况和植株农艺性状，收获时进行产量测定。结果表明：(1)多效唑浸种延缓花生种子萌发，使发芽率有所下降，矮化植株；(2)盛花期喷施适宜浓度的多效唑能抑制花生地上部分的生长，促进其横向生长，使主茎和分枝比对照的短，分枝数、单株结荚数、饱果率和产量均比对照的高，其中以喷施150mg／L多效唑的处理产量最高，达3806．3kg／hm^2，比对照增产12．60%。     

“绿色革命”以来大气CO2浓度升高对C3作物营养品质的影响

自20世纪60年代"绿色革命"以来,育种技术和农耕技术的发展促进了农作物产量的大幅提升,然而作物的营养品质出现下降趋势。在相似的遗传背景下,大气CO_2浓度升高会使单位体积农作物产品的营养元素含量下降,因此"绿色革命"至今,农作物产品的营养元素下降可能受大气CO_2浓度升高影响。通过植物生长箱模拟"绿色革命"初期和目前的大气CO_2浓度水平(310μmol/mol和400μmol/mol),针对主要C_3作物水稻、小麦和大豆,研究"绿色革命"以来大气CO_2浓度升高对其籽粒的C、N、Fe、Zn元素含量的影响,结果表明:CO_2浓度升高对3种作物籽粒的C元素含量几乎没有影响,变化幅度在±1.5%之间;籽粒的N、Fe、Zn元素含量普遍呈现下降趋势,但均未达到显著水平。     

Potential of wild species for genetic enhancement of some semi-arid food crops

Discovery and incorporation of genes from wild species provide means to sustain crop improvement, particularly when levels of resistance in the cultigens are low and virulent strains of pests and pathogens overcome the host plant resistance. The extent of utilization and the potential of the wild genepool for genetic enhancement were reviewed in five important food crops viz. sorghum, pearl millet, chickpea, pigeonpea and groundnut grown in the semi-arid tropics. Introgression from compatible wild germplasm in the primary gene pool resulted in transfer of new cytoplasmic male sterility systems in pearl millet and pigeonpea, development of high protein, cleistogamous flower and dwarf pigeonpea lines and foliar disease resistant groundnut cultivars. Utilization of wild species in secondary and tertiary gene pools has been generally limited due to sterility, restricted recombination or cross incompatibility. Nevertheless, these species are extremely important as they contain high levels of resistance to several important biotic and abiotic stresses. Several of them, like those belonging to the Parasorghum section and the rhizomatous Arachis species are sources of multiple resistances and hold great promise to sustain crop productivity.     

Faculty positions:Center for Agricultural Resources Research,Chinese Academy of Sciences

正The Center for Agricultural Resources Research(CARR),the Institute of Genetics and Developmental Biology(IGDB),Chinese Academy of Sciences,invites applicants for several research group leader positions.CARR is one of the research organizations in Chinese Academy of Sciences(CAS).We seek nominations and applications     

Soil-profile distribution of primary and secondary plant-available nutrients under conventional and no tillage

Nutrient distributions under no tillage (NT) compared with conventional disk-and-bed tillage (CT) management in the warm, humid region of the southeastern USA need to be assessed so that future placement, quantity, and type of fertilizers can be altered, if necessary, to efficiently match crop demands. We determined soil-profile distributions of pH, N, P, S, K, Ca, Mg, Na, Zn, Fe, Mn, and Cu to a depth of 0.9 m at the end of 8.5 years of continuous CT and NT management on a Weswood silty clay loam (fine, mixed, thermic Fluventic Ustochrept) in southcentral Texas. Most dramatic changes occurred within the 0–0.05 m depth, where soil under NT had lower pH, Fe, and Cu than under CT, but greater P, K, Zn, and Mn. Greater P and K under NT than under CT also occurred below the till-zone (0.15–0.3 m). At a depth of 0–0.3 m, soil under NT contained greater amounts of extractable P, K, Zn, Fe, Mn, and Cu than under CT. Nitrogen fertilization had little effect on nutrient distributions, except resulting in greater extractable K at 0–0.05 m and greater nitrate at 0–0.15 m. Few changes in soil-profile distributions were observed for extractable S, Ca, Mg, and Na. Long-term continuous use of NT on this fine-textured, high-fertility (except for N) soil had no apparent adverse effects on nutrient distributions relative to CT, but enhanced conservation and availability of P, K, Zn, Fe, Mn, and Cu near the soil surface where crop roots proliferate.     

Assessing the effectiveness of conservation measures: Resolving the “wicked” problem of the Steller sea lion

“Wicked” problems are those that are complex and that change when solutions are applied. Many conflicts in conservation fall into this category. The study approached the problem of how to constrain the apparent wickedness of a problem in the conservation management of a species by using simple empirical indicators to carry out iterative assessment of the risk to a population and to document how this risk evolves in relation to the addition of new data and the implementation of management actions. Effects of high levels of uncertainty within data and also concerning population structure were examined through stochastic simulation and by exploration of scenarios. Historical trends in the example used, the Steller sea lion, showed rapid declines in abundance in some regions during the 1980s. The current total population is 130,000-150,000 Steller sea lions through Alaska and British Columbia and this number has been stable since about 1990 in spite of regional differences in population dynamics. Regional differences in the sequence of changes in the number of pups and non-pups, suggested that an internal re-distribution of juveniles could have happened between 1980 and 1990. Current productivity also appears close to the long-term mean. Stochastic population projection using various scenarios showed that, based upon this history, the risk of extinction for the population has declined and is below reasonable thresholds for considering the population to be endangered. The trends in risk suggest that management actions taken since 1990 have probably been effective. Consequently, the conservation management objectives for the Steller sea lion are probably being met. The approach provides a mechanism, based upon experience and scenario analysis, for exploring future policy options and may help to constrain the debate amongst stakeholders about the cost-benefit trade-offs associated with different options.     

Collection and preliminary evaluation of native turfgrass accessions in Italy

Despite a raising interest on turfs in Italy, all theavailable varieties of this kind in the Country are of foreign origin, and areoften poorly adapted to the prevailing climatic conditions. This prompted tobegin a collection activity of indigenous turfgrass species, with the ultimategoal of identifying promising materials for future breeding based on localgenetic resources. The collection was carried out in three areas of Italy, viz.the northern Po Plain, the coastal region of Liguria, and the island of Sardiniathat are characterised, respectively, by subcontinental, warm temperate, andtypical Mediterranean climate. Altogether, 141 sites were visited, yielding 226accessions belonging to eight species of potential interest for turfs:Poa pratensis, Poa trivialis,Festuca rubra, Festuca arundinacea,Lolium perenne, Agrostis stolonifera,Agrostis tenuis, and Cynodon dactylon,this last being a warm-season grass. Poa pratensis andCynodon dactylon were mostly collected in northern Italyand Sardinia, respectively, whereas Festuca arundinacea andLolium perenne were rather ubiquitous. The collection sitesranged from 0 to 1040 m asl, but sites over 750 m wereonly visited in the inner part of Sardinia. All the accessions, collected aswhole plants, were transplanted at Lodi, northern Italy, where they are beingevaluated. Their preliminary evaluation for traits of importance for turf use,such as sward colour and overall quality, highlighted the great variation andthe occurrence of interesting accessions in all species. Other characters wererecorded, bearing specific importance in individual species, and in all casespromising accessions were identified. The germplasm of Festucarubra, Festuca arundinacea, and Loliumperenne proved highly infected by endophytic (symbiotic) fungi of thegenus Neotyphodium.     

Enhanced establishment and growth of giant cardon cactus in an eroded field in the Sonoran Desert using native legume trees as nurse plants aided by plant growth-promoting microorganisms and compost

To evaluate the feasibility of long-term desert reforestation technology of mixed vegetation, cardon cactus (Pachycereus pringlei) seedlings from indoor and outdoor nurseries were planted in the field adjacent to one seedling of potential legume nurse trees: mesquite amargo (Prosopis articulata), yellow palo verde (Parkinsonia microphylla), and blue palo verde (Parkinsonia florida). Some of the planting holes were also supplemented with common dairy compost. Additionally, the combinations of legume tree–cactus were inoculated with either a consortium of desert arbuscular mycorrhizal (AM) fungi, plant growth promoting bacteria (PGPB; the diazotroph Azospirillum brasilense Cd, and the phosphate solubilizer Paenibacillus sp.), or a mixture of all. The field experiments were evaluated periodically during 30 months for survival and growth. Cardons reared in an outdoor screen house survived better in the field than those reared in a controlled growth chamber and hardened later outdoors. Association with any legume nurse tree increased survival and enhanced growth of untreated cardons. For cardons growing alone, application of either compost, AM fungi, and all the treatments combined increased survival. For these plants, no treatment affected plant growth during the first 3 months after transplanting. Later, all treatments, except for AM fungi, enhanced plant growth. However, only 2 years after transplanting the enhanced growth effect of AM fungi was also significant. In the presence of the legume nurse trees, transient positive effects on cardon growth were recorded. General evaluation after 30 months of cultivation showed that the treatments positively affected cardon growth when growing alone or in combination only with mesquite amargo but not with the other two legume trees. This study proposes that young legume trees have the capacity to enhance survival and growth of cardon cactus, depending on the legume cactus combination. Additional treatments such as compost or PGPB can either amplify the effect or else attenuate it.     

Karyotypic and genome size variation in Cajanus cajan (L.) Millsp. (pigeonpea) and some wild relatives

Detailed karyotypes and 4C DNA amounts have been studied in five cultivars of Cajanus cajan and 20 species belonging to Cajanus, Rhynchosia, Dunbaria, Flemingia and Paracalyx. C. cajan shows intraspecific variability and its karyotype is most similar to that of C. cajanifolius (sect. Cajanus) in the morphology and number of satellite chromosomes and the lack of any correlation between chromosome size within the complement and asymmetry. Karyotypes of C. lineatus and C. sericeus belonging to sect. Atylia are similar with respect to maximum r-index and the ratio of longest and shortest chromosomes in their respective complements. C. acutifolius (sect. Frutocosa) is distinct in having a very low ratio (1.44) between the longest and the shortest chromosomes, while in C. albicans, C. goensis, C. scarabaeoides (sect. Cantharospermum) there is no chromosome pair with r-index >2.0. C. mollis and C. volubilis (sect. Volubilis) show similarity with regard to ratio between the longest and the shortest chromosomes and a distinct chromosome pair with an arm ratio of 2.7 and C. platycarpus is distinct (sect. Rhynchosoides) in having the smallest ratio (1.36) between the longest and the shortest chromosomes. 4C DNA amounts in Cajanus and Rhynchosia vary between 3.28 pg to 11.69 pg and 4.92 pg to 11.13 pg respectively, while in Dunbaria, Flemingia and Paracalyx these vary between 4.70 and 7:06 pg. In the genus Cajanus karyotypic features and 4C DNA amounts agree with the sectional classification and bring out a close relationship between C. cajan and C. cajanifolius. This is supported by studies based on seed protein patterns, isozyme analysis, trypsin and chymotrypsin inhibitor patterns, RFLP and RAPD data and crossability relationships. A clear relation between DNA amount and abnormalities effecting the extent of pairing and recombination in interspecific hybrids shows the importance of this study in developing future breeding programme involving C. cajan and its wild relatives that are of potential value.     

Effect of some fungicide seed treatments on the survival of Bradyrhizobium japonicum and on the nodulation and yield of soybean [Glycine max. (L) Merr.]

Several commercial fungicide seed treatments were evaluated for their possible effect on the survival of Bradyrhizobium japonicum on seeds and on the nodulation and yield of soybeans in a greenhouse and a field experiment. quinolate Pro (carbendazim and oxine copper), Vitavax 200FF (carboxin and thiram), and Monceren (pencycuron) had a small effect or no effect on the survival of B. japonicum and on the nodulation and yield of soybeans. They can thus be considered compatible with soybean seed inoculation. Germipro UFB (carbendazim and iprodione), Apron 35J (metalaxyl), and Tachigaren (hymexazol) decreased B. japonicum survival and the nodulation and yield of soybeans and thus cannot be considered compatible with soybean seed inoculation.     

Leaf position and genotype differences for mineral element concentrations in sorghum grown on tropical acid soil 1

Mineral element deficiencies and toxicities are common problems associated with sorghum [Sorghum bicolor (L.) Moench] production on acid soils. To better understand some of the mineral element problems and the analysis of plant tissue of sorghum plants grown on acid soils, four sorghum genotypes were grown on an acid Oxisol at Carimagua, Colombia limed with dolomite at 2 and 6 Mg ha^‐1.

Samples for mineral element analyses were obtained from leaves at different positions on the four genotypes. Concentrations of P and Mg were highest in the flag leaf (Leaf No. 1) and decreased as the position on the plant declined from the top of the plant for plants grown at 2 Mg lime ha^‐1. Similar decreases in P, Mg, K, and Zn concentrations occurred in plants grown with 6 Mg lime ha^‐1. Concentrations of Ca, S, Si, Mn, Fe, Cu, and Al increased as leaf position declined from the flag leaf for plants grown at 2 and 6 Mg lime ha^‐1. The higher lime supply enhanced Ca and reduced Mn and Fe concentrations in leaves. Differences in mineral element concentrations for the four genotypes used were fairly extensive. The elements to show the greatest range among genotypes were Al and Si and the elements to show the least range among genotypes were P, K, and S. Care should be used in collecting leaf samples for plant analysis and genotypic differences for accumulation of mineral elements should be considered in interpretation of results.