用茎瘤芥再生芽离体筛选抗S－（2－氨乙基）－L－半胱氨酸变异体研究

将茎瘤芥“棒棒菜”（Ｂｒａｓｓｉｃａｊｕｎｃｅａｖａｒ．ｔｕｍｉｄａｃｖ″Ｂａｎｇｂａｎｇｃａｉ″）下胚轴离休培养的再生芽，用１２０～４０Ｇｙγ射线照射后，在原培养基（ＭＳ＋ＮＡＡ０．２ｍｇ／ｌ＋ＢＡ２．０ｍｇ／ｌ）上培养１周，转到加Ｓ－（２－氨乙基）－Ｌ－半胱氨酸（ＡＥＣ）的原培养基中，经过连续７代选择，获得了抗ＡＥＣ的变异体植株。其蛋氨酸和精氨酸含量分别为对照的１．８４倍和３倍，赖氨酸比对照略有增加，总氨基酸含量为对照的１．４５倍。     

苹果属－亚种──中国苹果

苹果属－亚种──中国苹果李育农（西南农业大学）关键词：苹果属；西洋苹果；亚种ＭＡＬＵＳＤＯＭＥＳＴＩＣＡＳＵＢＳＰ．ＣＨＩＮＥＮＳＩＳＹ．Ｎ．ＬＩ．－ＡＳＵＢＳＰＥＣＩＥＳＯＦＭＡＬＵＳＭＩＬＬ．￥ＬｉＹｕｎｏｎｇ（ＳｏｕｔｈｗｅｓｔＡｇｒｉｃｕｌｔ...     

Anatomical Structure of Root and Root Nodule of Alnus sibirica-Host o fBoschniakia rosica

ＡｎａｔｏｍｉｃａｌＳｔｒｕｃｔｕｒｅｏｆＲｏｏｔａｎｄＲｏｏｔＮｏｄｕｌｅｏｆＡｌｎｕｓｓｉｂｉｒｉｃａ－ＨｏｓｔｏｆＢｏｓｃｈｎｉａｋｉａｒｏｓｉｃａＨｕＢａｏｚｈｏｎｇ，ＬｉＪｉｎｇ，ＧｕｉＭｉｎｇｚｈｕ，ＬｉＧｕｉｑｉｎａｎｄＸｕＬｉｊｕｎ（...     

Plant Regeneration of Irradiated Barley Haploid Calus Derived by Bulbosum Method

ＰｌａｎｔＲｅｇｅｎｅｒａｔｉｏｎｏｆＩｒｒａｄｉａｔｅｄＢａｒｌｅｙＨａｐｌｏｉｄＣａｌｕｓＤｅｒｉｖｅｄｂｙＢｕｌｂｏｓｕｍＭｅｔｈｏｄＷａｎｇＧｕａｎｇｊｉｎ（ＨｅｉｌｏｎｇｊｉａｎｇＡｃａｄｅｍｙｏｆＡｇｒｉｃｕｌｔｕｒａｌＳｃｉｅｎｃｅｓ，...     

小麦转基因植株的后代鉴定及遗传学分析

偏、粘、易型非１Ｂ／１Ｒ小麦雄性不育系育成ＢｒｅｅｄｉｎｇｏｆＭａｌｅＳｔｅｒｉｌｅＬｉｎｅｓｏｆＮｏｎ－１Ｂ／１ＲＷｈｅａｔｗｉｔｈＡｅ．ｖｅｎｔｒｉｃｏｓａ，Ａｅ．ｋｏｔｓｃｈｙｉａｎｄＡｅ．ｖａｒｉａｂｉｌｉｓＣｙｔｏｐｌａｓｍｓ实践证明，近年...     

Biological Effects of Deer Semen on Development of Testes in Mice

ＢｉｏｌｏｇｉｃａｌＥｆｆｅｃｔｓｏｆＤｅｅｒＳｅｍｅｎｏｎＤｅｖｅｌｏｐｍｅｎｔｏｆＴｅｓｔｅｓｉｎＭｉｃｅ＊ＬｉａｎＺｈｅｎｇｘｉｎｇ，ＬｉＷｕ（ＮｏｒｔｈｅａｓｔＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ，Ｈａｒｂｉｎ１５００３０，ＰＲＣ）Ａ...     

The Resumption of Ovarian Activity in Postpartum of Chinese—Holstein Cows

ＴｈｅＲｅｓｕｍｐｔｉｏｎｏｆＯｖａｒｉａｎＡｃｔｉｖｉｔｙｉｎＰｏｓｔｐａｒｔｕｍｏｆＣｈｉｎｅｓｅ－ＨｏｌｓｔｅｉｎＣｏｗｓＺｈｏｕＨｏｎｇｚｈｅ，ＴｉａｎＷｅｎｒｕ（ＮｏｒｔｈｅａｓｔＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ，Ｈａｒｂｉｎ...     

The Efect of High Temperature and Clenb-uterol on Performance and Carcas Composition of Broilers

ＴｈｅＥｆｅｃｔｏｆＨｉｇｈＴｅｍｐｅｒａｔｕｒｅａｎｄＣｌｅｎｂ－ｕｔｅｒｏｌｏｎＰｅｒｆｏｒｍａｎｃｅａｎｄＣａｒｃａｓｓＣｏｍｐｏｓｉｔｉｏｎｏｆＢｒｏｉｌｅｒｓＬｉＸｕｅ，ＬｉＹｕｚｈｉ，ＷａｎｇＱｉｎｇｈａｏ，ＭｅｎｇＸｉａｎｇｆｅｎｇ，Ｓ...     

On the Analysis Method of the Triple Test Cross Design

ＯｎｔｈｅＡｎａｌｙｓｉｓＭｅｔｈｏｄｏｆｔｈｅＴｒｉｐｌｅＴｅｓｔＣｒｏｓｓＤｅｓｉｇｎ￥ＪｉｎＹｉ（ＮｏｒｔｈｅａｓｔＡｇｒｉｃｕｌｔｕｒａｌＵｎｉｖｅｒｓｉｔｙ，Ｈａｒｂｉｎ１５００３０，ＰＲＣ）Ａｂｓｔｒａｃｔ：Ｔｈｅａｎａｌｙｓｉｓｍｅｔｈ...     

Trace Residue Analysis of the Herbicide Imazethapyr in Soil by Gas Chromatography-Nitrogen/Phosphorous Detector

ＴｒａｃｅＲｅｓｉｄｕｅＡｎａｌｙｓｉｓｏｆｔｈｅＨｅｒｂｉｃｉｄｅＩｍａｚｅｔｈａｐｙｒｉｎＳｏｉｌｂｙＧａｓＣｈｒｏｍａｔｏｇｒａｐｈｙ－Ｎｉｔｒｏｇｅｎ／ＰｈｏｓｐｈｏｒｏｕｓＤｅｔｅｃｔｏｒＸｉａｎｇＷｅｎｓｈｅｎｇ，ＳｕＳｈａｏｑｕａｎ，Ｗ...     

犊牛腹泻轮状病毒在3个传代细胞上培养特性的研究

对犊牛腹泻轮状病毒（ＮＣＤＶ）在３种传代细胞系上进行了培养，并将其培养特性进行了比较研究。结果表明：犊牛腹泻轮状病毒在ＭＡ－１０４细胞上生长，其病毒抗原的生长部位在细胞浆，培养后可出现细胞病变；犊牛腹泻轮状病毒在ＬＬｃ－ｍｋ２细胞上也生长，且随着培养代次的增加，病毒抗原的滴度相应增加，增长到一定滴定后即较稳定。     

Effects of Matrine on Bovine Papilloma Virus-infected C127 Cells

The aim of this study was to select drugs for prevention and treatment of bovine papilloma in vitro, using bovine papilloma virus(BPV-2) infected mouse mammary epithelial(C127) cell lines as the infectious cell model system. C127 cell shapes changed with BPV infection observed under light microscopy. C127 cell shapes changed with BPV infection observed under light microscopy. PCR was used to confirm BPV infected C127 cells. mR NA levels of matrine to BPV-2 infected C127 cells were detected by RT-PCR. Apoptosis of BPV-infected C127 cells treated with matrine was analyzed by flow cytometry. Results showed that 80 μg· mL~(-1) matrine had a direct virucidal and intervention effect of BPV-2. Matrine inhibited the proliferation of BPV-infected cells. Therefore, matrine might be regarded as a therapeutic drug candidate for BPV induced bovine papilloma disease treatment in future.     

视网膜色素上皮细胞光损伤模型的建立与评估

目的 通过不同光照时间和光照强度,探索建立最佳视网膜色素上皮细胞光损伤模型,为视网膜光损伤细胞造模方法提供理论依据。方法 选取原代大鼠视网膜色素上皮细胞和大鼠视网膜色素上皮细胞系,LED白色冷光灯模拟自然光源,选取（2 500、5 000、7 500、10 000 Lux）作为光照强度,以不同光照时间（6、12 h） 对细胞进行照射,建立光损伤模型,镜下观察细胞形态,采用MTT法检测细胞活力。结果 光照处理后,RPE细胞活力受到抑制,随着光照强度及时间增强,细胞活力降低明显,原代细胞6 h 7 500、10 000 Lux和12 h 5 000、7 500、10 000 Lux,细胞系6 h 10 000 Lux和12 h 7 500、10 000 Lux,视网膜色素上皮细胞活力均低于空白组（P<0.01）,差异有统计学意义。结论 RPE细胞活力受光照时间及强度抑制,原代细胞较细胞系对光损伤更敏感。     

我国两系法杂交水稻研究的形势,任务和发展前景

从育种上讲，杂交水稻育种可以分为三系法、两系法和一系法三个发展阶段；从利用杂种优势水平来看，杂交水稻育种可以分为品种间、亚种间和远缘杂种优势利用三个发展阶段。与三系法比较，两系法具有配组自由、种子生产成本低、无不育细胞质负效应、易转育新不育系等优点。迄今为止，我国已育成２０多个通过省级鉴定的实用光温敏核不育系，７个通过省级审定的两系法杂交稻组合，解决了低温敏不育系繁殖产量低而不稳的难题，实施了光温     

Functional properties of antigen-specific T cells infected by human T-cell leukemia-lymphoma virus (HTLV-I)

Tetanus-toxoid specific helper-inducer T-cell clones, which had been infected and transformed by human T-cell leukemia-lymphoma virus (HTLV-I), were obtained from an antigen-specific human T cell line by using a limiting dilution technique in the presence of the virus. These HTLV-I-infected T-cell clones proliferated specifically in response to soluble tetanus toxoid but, unlike normal T cells, they could do so in the absence of accessory cells. The HTLV-I-infected T-cell clones did not present the antigen to autologous antigen-specific T cells that were not infected with HTLV-I. The capacity of helper-inducer T cells to retain antigen-specific reactivity after infection by HTLV-I, while losing the normal T-cell requirement for accessory cells, has clinical and theoretical implications.     

薯蓣皂苷元对HL-60细胞增殖及细胞周期的影响

采用XTT比色法和流式细胞仪技术观察薯蓣皂苷元(Diosgenin，Dio)对人急性髓性白血病细胞株HL-60的增殖抑制作用以及细胞周期变化。结果表明：XTT法检测其24h的半数抑制浓度(IC50)为7．36mg/L．流式细胞仪检测Dio作用24h后，G0/Gl期细胞增多，S期细胞减少，凋亡细胞增多。Dio可明显抑制HL-60细胞的增殖。     

E2蛋白介导猪瘟病毒的细胞吸附与内化

【目的】已有研究显示,猪瘟病毒(CSFV)通过网格蛋白介导的内吞作用侵入宿主细胞,然而参与侵入过程的病毒蛋白尚待阐明。研究旨在明确E2蛋白在CSFV侵入过程中的作用。【方法】通过瞬时转染包装携带E2基因的慢病毒,将慢病毒转导悬浮293细胞构建稳定表达重组E2蛋白的悬浮293细胞系,利用亲和层析的方法纯化重组E2蛋白,同时优化表达时间以增加蛋白产量。利用SDS-PAGE和Western blotting分别鉴定了重组E2蛋白的表达水平及其反应原性。通过感染试验、吸附试验以及内化试验分别探究了E2蛋白对CSFV感染、吸附以及内化的影响。将重组E2蛋白免疫BALB/c小鼠制备多克隆抗体,通过阻断ELISA检测血清中多克隆抗体的阻断率。利用制备的多克隆抗体与CSFV感作后进行吸附和内化试验,进一步探究了E2蛋白在介导CSFV吸附和内化中的作用。【结果】通过倒置荧光显微镜观察转导慢病毒后的细胞系,与对照细胞相比可见明显的绿色荧光,表明慢病毒成功转导悬浮293细胞。SDS-PAGE结果显示,在还原和非还原条件下均出现与预期大小相符的蛋白条带,经Western blotting验证,上清中表达的重组E2蛋白能够被抗E2蛋白单克隆抗体WH303所识别,表明构建的悬浮293细胞系能够表达重组E2蛋白。通过优化表达条件,悬浮293细胞培养第8天时上清中重组E2蛋白浓度最高可达5.84μg·m L-1。可溶性蛋白阻断试验结果显示,重组E2蛋白具有阻断CSFV感染的活性。利用重组E2蛋白在病毒入侵过程中处理细胞对CSFV的感染同样具有显著抑制作用;阻断ELISA和中和试验结果显示,针对E2蛋白的多克隆抗体能够阻断CSFV感染,且具有良好的中和活性;吸附试验和内化试验证明,重组E2蛋白处理细胞能够显著抑制CSFV的内化,而利用多克隆抗体与CSFV感作后能够显著抑制其吸附。【结论】E2蛋白参与CSFV吸附和内化。     

Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV

Cells infected with the human immunodeficiency virus (HIV) show decreased expression of the 58-kilodalton T4 (CD4) antigen on their surface. In this study, the effect of HIV infection on the synthesis of T4 messenger RNA (mRNA) and protein products was evaluated in T-cell lines. Metabolically labeled lysates from the T4+ cell line Sup-T1 were immunoprecipitated with monoclonal antibodies to T4. Compared with uninfected cells, HIV-infected Sup-T1 cells showed decreased amounts of T4 that coprecipitated with both the 120-kilodalton viral envelope and the 150-kilodalton envelope precursor molecules. In four of five HIV-producing T-cell lines studied, the steady-state levels of T4 mRNA were also reduced. Thus, the decreased T4 antigen on HIV-infected cells is due to at least three factors: reduced steady-state levels of T4-specific mRNA, reduced amounts of immunoprecipitable T4 antigen, and the complexing of available T4 antigen with viral envelope gene products. The data suggested that the T4 protein produced after infection may be complexed with viral envelope gene products within infected cells. Retroviral envelope-receptor complexes may thus participate in a general mechanism by which receptors for retroviruses are down-modulated and alterations in cellular function develop after infection.     

Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line

Human T-cell leukemia virus (HTLV), American PL isolate, was transmitted by cocultivation and by cell-free filtrates to a nonlymphoid human osteogenic sarcoma (HOS) cell line, designated HOS/PL, but not to nine other lines bearing receptors for HTLV. HOS and HOS/PL cells are not dependent on interleukin-2 and do not express interleukin-2 receptors that are recognized by anti-Tac monoclonal antibody. HTLV released by the Japanese MT2 cell line was also transmitted to HOS cells. The infected HOS cells release substantial titers of progeny HTLV which is antigenically indistinguishable from parental virus and is able to transform T cells.