贵阳地区猪弓形虫病的血清学检测方法分析

为全面了解贵阳市地区猪弓形虫感染情况,于2014年4月～2015年5月分别采集贵阳地区猪血清样本共984份。采用弓形虫间接血凝试验（IHA）和弓形虫IgG抗体ELISA两种方法来检测。结果显示,两种方法存在关联性,前者阳性率为17.37%,后者阳性率为20.73%。表明贵阳地区不同类别的养猪场户存在弓形虫病感染,此外,ELISA方法检测弓形虫抗体检出率高于间接血凝试验。     

贵州省猪弓形虫病的血清学调查

为全面了解贵州省猪弓形虫感染情况,对采自9个市（州、地）264个养猪场（户）的2 906份血清用酶联免疫吸附试验（ELISA）检测猪弓形虫抗体,总体阳性率为65.83%,变化范围为27.88%~85.42%。采集65份猪血清做ELISA和间接血凝试验（indirect heamagglutination assay,IHA）比较测定,两种方法总体符合率为58.46%。IHA方法补充检测177份猪血清,弓形虫抗体阳性率为27.68%。血清学调查结果与国内部分省（市）报道相符。     

贵州省猪弓形虫病的血清学调查

近年来,猪弓形虫感染全国都较严重,国内部分省市（浙江省、辽宁省、山东省、河北省、上海地区）猪弓形虫抗体间接血凝试验（IHA）检测阳性率为29.7%～66.7%,重庆地区酶联免疫吸附试验（ELISA）检测阳性率为60.42%。     

松江区家禽弓形虫感染的血清学调查

应用间接血凝试验(IHA)对松江区13个镇(街道)的禽类养殖户及3个规模养鸡场的580份禽类血清进行了弓形虫感染抗体检测,共检出抗体阳性血清91份,总抗体阳性率为15.69%(其中鸡抗体阳性率为10.03%、鸭抗体阳性率为26.54%、鸽抗体阳性率为5.0%)。结果表明,松江区禽类养殖场(户)均存在不同程度的弓形虫感染,且散养鸡的阳性率明显高于规模场鸡,散养鸭的阳性率明显高于散养鸡。     

猪O型口蹄疫3种抗体检测方法的比较

用液相阻断ELISA、正向间接血凝试验、VP1结构蛋白ELISA3种抗体检测方法同时检测来自全国10个省25个规模猪场、18个屠宰场及散养户的937份猪血清中的O型口蹄疫抗体。结果表明,这3种方法检测的抗体阳性率分别为75.9%、81.8%和62%。与液相阻断ELISA相比,正向间接血凝试验比VP1结构蛋白ELISA的符合率高,前者的敏感性为94.9%,特异性为59.3%,后者的敏感性为75.2%,特异性为79.6%。研究为猪O型口蹄疫免疫抗体监测工作的实施提供了较好的参考。     

内蒙古部分地区放牧牛羊弓形虫血清学调查

[目的]了解内蒙古部分地区放牧牛羊弓形虫病感染情况。[方法]采用间接血凝试验(IHA)对阿拉善盟及呼伦贝尔市随机采集的286份放牧牛羊血清样本进行弓形虫抗体检测。[结果]绵羊血清弓形虫抗体总阳性率为1.68%,其中,阿拉善盟阳性率为4.84%,呼伦贝尔市阳性率为0.56%;经统计学分析,两个地区的绵羊血清弓形虫抗体阳性率差异不显著(P>0.05);牛血清弓形虫抗体总阳性率为0。[结论]内蒙古主要牧区放牧绵羊存在弓形虫感染,应引起足够的重视,并制定相应的弓形虫病防治措施。     

河北省猪弓形虫的血清学检测

应用间接血凝试验(IHA)对采自我省11个地区定点屠宰厂,养殖场及散养户的1300份猪血清进行了弓形虫病抗体检测,结果表明,阳性464份,阳性率为35.69%。     

辽宁省盖州市绒山羊弓形虫病的血清学调查

应用弓形虫的间接血凝试验(IHA),对来自辽宁省盖州市的360份绒山羊的血清,进行弓形虫间接血凝抗体的检测。结果检出15份阳性血清,血清阳性率为4.17%。     

羊流产嗜衣原体病和弓形虫病IHA与ELISA诊断方法的比较

通过比较研究羊流产嗜衣原体与弓形虫抗体的间接血凝试验(IHA)与酶联免疫吸附试验(ELISA)检测结果,选择适宜贵州省山羊流产血清学调查的检测方法。通过IHA和ELISA两种方法对贵州省195份山羊血清进行羊流产嗜衣原体与弓形虫抗体的检测,统计并分析两种疫病的阳性符合率、阴性符合率和总符合率。结果表明,羊流产嗜衣原体与弓形虫IHA与ELISA的总符合率分别为77.95%和78.97%,阳性符合率均仅为50%,阴性符合率为80.45%和79.27%。说明在检测两种疫病血清抗体方面,IHA比ELISA更适合在贵州省基层推广。     

青海省天峻县藏系羊弓形虫病的血清学调查

应用弓形虫的间接血凝试验(IHA),对来自青海省天峻县的360份藏系绵羊的血清,进行弓形虫间接血凝抗体的检测。结果检出10份阳性血清,血清阳性率为2.78%。     

喜马拉雅旱獭弓形虫病血清学检测

用弓形虫的重组蛋白GST-SAG1作为ELISA诊断抗原,采用ELISA检测方法,对青海省祁连县喜马拉雅旱獭血清进行了弓形虫病的血清学检测。结果表明,被检92份喜马拉雅旱獭共检出阳性血清25份,阳性率约为27.17%。结果提示,青海省祁连县的喜马拉雅旱獭中存在弓形虫病的感染。     

青海省牛弓形虫病的ELISA诊断及试剂盒建立

用弓形虫的重组蛋白SAG1作为ELISA诊断抗原,建立了ELISA诊断试剂盒,并对青海省牛群进行弓形虫病的血清学诊断。经对所收集的1856份牛血清进行弓形虫抗体检测,共检出阳性血清71份,阳性率约为3.83%。结果表明青海省牛群中存在弓形虫病的感染。     

犬弓形虫抗体间接ELISA检测方法的建立

试验以利用重组弓形虫SAG1基因转染蜥蜴利什曼原虫所表达获得的目的蛋白作为包被抗原,建立了一种快速、特异、可检测犬弓形虫抗体的间接ELISA方法。确定了抗原最佳包被浓度为6.75 μg/mL,血清最佳稀释度为1∶100,对已知阳性血清检测的下限可达1∶6400,批间和批内重复性试验的变异系数均小于10%,包被抗原的酶标板在4、-20 ℃环境中可保存8个月以上。建立的间接ELISA方法应用于犬弓形虫抗体的检测具有较好的敏感性及特异性。     

几种检测猪弓形虫病ELISA诊断试剂盒的对比

以人工感染弓形虫的猪阳性血清及感染前的阴性血清为样品,比较3个国产(A、B、C)和2个国外(D、E)ELISA试剂盒检测猪弓形虫病的敏感性。用这5种ELISA试剂盒,检测人工感染弓形虫的猪阳性血清42份及感染前的阴性血清45份,并参考PCR扩增结果,比较这5种ELISA试剂盒检测猪弓形虫病的敏感性。5种试剂盒中,1种国产试剂盒的检测结果与PCR结果完全一致,其余4种试剂盒的检测结果都与PCR检测结果差别较大。结果表明,国产试剂盒A的检测效果最佳,国外试剂盒与部分国内试剂盒的检测结果相当,临床选用试剂盒时,可选择检测效果最佳的试剂盒A,不必盲目选择国外试剂盒。     

成都市区犬猫狂犬病病毒和弓形虫感染的调查

为了解四川省成都市区家养犬、猫狂犬病病毒及弓形虫的感染情况,采用商品化狂犬病病毒和弓形虫抗原快速检测试纸卡,对2010年8～10月间来自成都市区的103份家养犬以及75份家猫的唾液和血液样品进行检测;同时采用文献报道的PCR方法对家养犬血液样品中的弓形虫核酸进行检测。结果显示,103份家养犬唾液样品狂犬病病毒抗原均为阴性,而75份家猫唾液样品中,检出阳性样品5份(阳性率6.7%),可疑样品7份;103份家养犬血液样品弓形虫抗原阳性样品33份(32.0%),可疑样品22份,75份家猫血液样品中,检出阳性样品2份(阳性率2.7%),可疑样品3份。弓形虫核酸PCR检测结果显示,96份家养犬血液样品弓形虫核酸阳性样品57份(阳性率59.4%),与弓形虫抗原阳性和可疑样品总和所占比例基本一致(53.4%)。提示应重视源于家猫的狂犬病病毒和家养犬弓形虫对人的威胁。     

检测实验动物弓形虫感染的两种PCR方法的建立和比较

为了建立敏感、稳定、特异的PCR检测体系，用于实验动物弓形虫感染的检测。采用B1基因设计引物，建立常规PCR，用P30基因设计引物，建立巢式PCR；用两种PCR方法检测实验感染弓形虫小鼠血液和腹腔波中的DNA动态变化；用巢式PCR检测自然状态下的普通级豚鼠、教学和科研用兔的弓形虫感染率，并和常规PCR检到结果比较。结果，巢式PCR可检测到1fgDNA含量，比常规PCR敏感lOO倍；对其他微生物DNA无交叉现象，特异性强；对同一样品重复检测3次，阴、阳性结果一致，稳定性好。小鼠感染弓形虫2d后，巢式PCR对小民腹腔液的阳性检出率为83．3％，对血液的阳性检出率为33．3％；感染3d后，腹腔液阳性检出率为100％；而常规PCR在小鼠感染3d和4d后才能在腹腔液和血液中检测到，检出率各为16．7％。受检普通级豚鼠没有感染弓形虫，教学和科研用兔的弓形虫总感染率为14．3％。结论认为，巢式PCR方法可用于实验动物弓形虫早期感染的检测，具有敏感性高、特务性强、稳定性好的特点。     

新疆部分地区规模化猪场弓形虫抗体监测与分析

为了查明新疆部分地区规模化猪场弓形虫流行情况,应用酶联免疫吸附试验对5个猪场随机采集的173分血清样品进行弓形虫抗体监测。结果表明,猪弓形虫抗体阳性率高达82.7%(143/173),其中3个猪场的后备母猪的弓形虫抗体阳性率高达100%,5个猪场公猪的弓形虫抗体阳性率在85%~75%之间。     

Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig

The modified agglutination test (MAT) and a commercially available enzyme-linked immunosorbent assay (ELISA) were compared for detection of antibodies to Toxoplasma gondii in naturally-infected market-aged pigs. Infected pigs were obtained from commercial slaughter facilities and from farms where infection had previously been detected. Infection was confirmed by bioassay in cats. For 70 bioassay positive pigs, 60 were positive by MAT (85.7% sensitivity) and 62 were positive by ELISA (88.6% sensitivity). Of 204 bioassay negative samples 193 were negative by MAT (94.6% specificity) and 200 were negative by ELISA (98.0% specificity). Good correlation was seen between MAT and ELISA results. The results suggest that the ELISA may be a good tool for epidemiological studies of Toxoplasma infection on pig farms.     

A serological study on the prevalence of Toxoplasma gondii in sheep of Lithuania

In the present study the seroprevalence of the protozoan parasite Toxoplasma gondii infection in sheep was investigated in 6 regions of Lithuania. Blood samples were taken from 354 sheep and were tested using commercial ELISA method. The total seroprevalence of Toxoplasma gondii infection in sheep was 42.1%. Significant differences in seroprevalence were observed between age groups (P < or = 0.05). The results of this investigation suggest that the Toxoplasma gondii parasite is widely spread, and can be one of reasons of sheep abortion in Lithuania.     

Toxoplasmosis and border disease in 54 Swedish sheep flocks. Seroprevalence and incidence during one gestation period.

Serum samples from 704 animals from 54 Swedish sheep flocks were analysed by ELISA twice during 1 breeding season for antibodies to Toxoplasma gondii and border disease virus (BDV). An ELISA, originally developed for the detection of antibodies to bovine viral diarrhoea virus (BVDV) in cattle, was assessed on sheep sera and the results were compared with those obtained in a virus neutralization test. The correlation between the 2 assays proved good. Before breeding, 132 (19%) sheep in 42 flocks had antibodies to T. gondii and 7 (1%) sheep in 5 flocks were seropositive to BDV. During the observation period 4 sheep seroconverted to T. gondii and 13 to BDV, giving an incidence rate of 0.7% and 1.9% respectively. No clinical signs due to the infections were observed. In 5 flocks the frequency of barrenness, abortion or stillbirths exceeded 5%, 5% and 8%, respectively, but there was no evidence that this was attributable to the agents studied. The proportion of BDV-positive flocks was significantly higher among flocks that had been in contact with cattle than among those that had not.