He—Ne激光免疫调节机理研究

应用不同剂量的Ｈｅ－Ｎｅ激光照射大鼠脊背正中毛皮肤，连续１４ｄ，观察不同时间；２６．４８、３９．７２Ｊ／ｃｍ＾２的Ｈｅ－Ｎｅ激光对上述指标具有一定程度的促进作用，２６．４８，３９．７２Ｊ／ｃｍ＾２的Ｈｅ－Ｎｅ激光对上述指标具有一定程度的抑制作用。     

激光促进羊创伤愈合的病理学研究

１６只绵羊造成皮肤全层缺损创，用７．５３焦耳／ｃｍ＾２的氦氖激光照射，进行了创伤形态学，组织病理学和抗张强度的分析。结果表明激光能使创伤提前３．２５天愈合，上皮化过程提前３－４天完成，并加快毛细血管的生长和生发层细胞的再生，增加胶原纤维的数量和肉芽组织的形成，使胶原纤维的排列和力的方向一致，新生组织质地紧密，实验组比对照组抗张强度增加１．０６公斤／ｃｍ＾２。     

贵州足球场草坪建植及优化管理模型研究

在一定的生境和管理条件下,以修剪高度、修剪次数和施Ｎ、Ｐ、Ｋ量为因素,利用二次正交回归设计,用模糊综合评分法评定草坪质量,最佳３因子水平为：修剪高度５－７．４ｃｍ,修剪次数４－６次／月,施Ｎ量为２．５－３．７ｇ（ｍ＾２．年）,施Ｐ、Ｋ量各为１．２５－１．８５ｇ／（ｍ＾２．年）,建立了草坪质量与养护３因子间的显相关回归模型。     

大剂量激光,免疫抑制剂及两者配合对大鼠细胞免疫的影响

应用３９．７２４５Ｊ／ｃｍ＾２的Ｈｅ－ＮＥ激光连续１４ｄ照射健康大鼠脊背正中线皮肤、并同时分别与亚剂量的免疫抑制剂环孢霉素、硫唑嘌呤配合，观察它们对大鼠Ｔ，Ｂ淋巴细胞数、淋巴细胞转化刺激指数、１０６个淋巴细胞ＣＰＭ数的影响，结果表明：大剂量激光和Ａｚａ及激光与二者配合均可使淋巴细胞转化刺激指数在试验后４周内较试验前明显降低。     

激光照射家畜创伤对血清蛋白作用的研究

用７．５２Ｊ／ｃｍ＾２氦氖激光照射绵羊的皮肤缺损创，测定了血清总蛋白、白蛋白、α１球蛋白、α２球蛋白、β球蛋白、γ球蛋白、血红蛋白含量及Ａ／Ｇ值与血清ｐＨ值。结果表明，激光照射能显著提高血清总蛋白（５．４３％）和血红蛋白（４．１２％）含量，增加球蛋白（１４．１６％），尤其是γ球蛋白（２６．６７％）的百分比，相应减少白蛋白的比值，对血清ｐＨ无明显作用。这些变化在创伤后１５－２１天达高峰，说明激光照射     

猪细小病毒生物学特性研究

应用ＰＫ－１５传代细胞复制猪细小病毒（ＰＰＶ）参考株ＮＡＤＬ－２和云南分离株ＫＭ，研究得出细胞培养物半数细胞感染量分别为１０＾－７．９／ｍｌ和１０＾－６．８／ｍｌ经ＣｓＣｌ密度梯度离心，两种病毒颗粒其浮力密度值分别为１．３３ｇ／ｃｍ＾３和１．３９／ｃｍ，电镜观察病毒粒子呈圆形，直径约２０ｎｍ。ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）分析，表明纯化的ＰＰＶ细胞培养毒，主要含两种蛋白成分，分子量     

NJS系小鼠的多项遗传纯度监测与分析

对作者培育的ＮＪＳ小鼠进行了生化标志基因测试、免疫标志基因测试、毛色基因测试、皮肤移植试验、下颌骨形态分析等多项遗传监测。结果表明，控制ＮＪＳ小鼠的生化、免疫、形态等遗传学性状的基因位点均已纯合，该小鼠符合近交系动物的遗传纯度要求。ＮＪＳ小鼠是一株新培育的近交系，具有独特的遗传概貌和特性。所检的分布在１２条染色体上的２５个生化标志基因未见与其他近交系小鼠完全相同。Ｈ－２位点基因为Ｈ－２Ｋｄ、Ｈ－２Ｄｋ。毛色基因型为ＡＡＢＢｃｃＤＤ。下颌骨形态判别函数ＤＦ１、ＤＦ２、ＤＦ３、ＤＦ４值分别为４．５１、－６．３１、０．１６、－０．８５。与近交系ＢＡＬＢ／ｃ、Ｃ５７ＢＬ／６、６１５、ＳＭＭＣ／ｃ的遗传距离分别为８３．１９、６２．８７、４１．３４、２２．４４。     

白草群落生物量初步研究

研究了黄土区白草群落６月和７月的生物量，测定结果：６月和７月地上生物量分别为９０．９７ｇ／ｍ＾２和９５．０２ｇ／ｍ＾２，地下生物量为３２４．００ｇ／ｍ＾２和４５２．６１ｇ／ｍ＾２。地上生物量积累很少，地下生物量积累较多，该现象决定于白草生长发育特点。     

重组牛白细胞介素-2增强小鼠对伊氏锥虫可溶性抗原免疫应答的研究

使用伊氏锥虫可溶性抗原免疫小鼠，研究了同时注射重组牛白细胞介素－２（ｒＢＩＬ－２）对ＮＫ细胞杀伤活性及特异性抗体水平的影响。试验鼠随机分为４组：Ａｇ组，ｒＢＩＬ－２组，Ａｇ＋ｒＢＩＬ－２组和ＰＢＳ对照组。以ＬＤＨ释放法和间接ＥＬＩＳＡ法分别检测脾ＮＫ细胞杀伤活性及抗体水平。结果表明，首次免疫后２４ｄ，Ａｇ组ＮＫ细胞杀伤活性为３１．６％，ｒＢＩＬ－２组为９９．６６％，Ａｇ＋ｒＢＩＬ－２组为９８．９８％，对照组为１９．７３％；抗体效价Ａｇ组为２４，Ａｇ＋ｒＢＩＬ－２组为２６。说明ｒＢＩＬ－２可显著提高ＮＫ细胞杀伤活性及促进抗体生成，提高宿主免疫力。     

抗鸡IgG重链单克隆抗体的制备与应用

以提纯鸡ＩｇＧ做抗原免疫Ｂａｌｌ／ｃ小鼠，取鼠细胞在ＰＥＧ１０００作用下与小鼠骨髓细胞（Ｓｐ２／ｏＡｇ１４）融合，采用间接免疫荧光（ＩＦＡ）和酶联免疫吸附试验（ＥＬＩＳＡ）检测上清抗体，阳性孔经有限稀法进行细胞克隆，共获得了７株分泌抗鸡ＩｇＧ单克隆抗体的杂交瘤细胞（２Ｈ８、４Ｄ８、４Ｄ８、１Ｂ７、２Ａ７、２Ｂ３、１Ｇ１２、３Ｄ１２）将这些细胞分别接种间系小鼠制备出腹水抗体，ＥＬＩＳＡ效价可达１０＾     

氦氖激光合用环磷酰胺对S180荷瘤鼠脾脏淋巴细胞IL-2诱生活性和增殖反应的影响

以S180腹水瘤小鼠为动物模型，应用11.00、14.67和22.00J/cm^2 3种剂量氦氖激光照射荷瘤鼠哈德腺，同时联合应用化疗药物环磷酰胺，对S180荷瘤鼠脾脏淋巴细胞IL-2诱生活性和淋巴细胞增殖反应的影响作了系统地研究。结果表明：CYT/氦氖激光照射联合应用组IL-2诱生活性和淋巴细胞增殖反应均明显高于CYT对照组。CYT可对荷瘤体产生某种程度的免疫抑制效应，而氦氖激光照射则可在某种程度上改善这一效应。     

低功率氦氖激光加血卟啉衍生物对动物移植肿瘤的疗效观察

用血卟啉衍生物结合氦氖激光,治疗小鼠S180纤维肉瘤。其显效率高达74.51％,肿瘤消失率达64.71％;在治疗后第13、19天,肿瘤的平均重量和体积分别与治疗前比较,差异极显著(P<0.01);当激光剂量不变时,随着血卟啉衍生物用量增加,其疗效增加。     

激光照射对雄性梅花鹿睾酮,雌二醇含量影响的试验

本文以８头雄性梅花鹿为材料，应用输出功率１７ｍｗ，能量密度为５７．７２Ｊ／ｃｍ２的氦氖激光对其中４头进行照射试验；应用放射免疫测定法测其外周血清中睾酮、雌二醇含量的变化。结果表明：激光照射能够影响梅花鹿外周血清中性激素水平，使睾酮和雌二醇水平显著下降（Ｐ＜０．０５），睾酮降低４１．２５％，雌二醇降低４３．５５％。     

Immunologic and genetic characterization of S180, a cell line of murine origin capable of growing in different inbred strains of mice

Some of the immunologic and genetic properties of the cell line S180 have been examined. These cells grew without restrictions in the peritoneal cavity of different inbred strains of mice and invariably killed the animals. With Northern blots it was demonstrated that S180 cells contained class I mRNAs but failed to transcribe B2m genes. However, under experimental conditions, a protective humoral immune response mediated by cytotoxic antibodies and complement against S180 cells was obtained through non-H-2 antigens in C57BL/6J mice.     

Defocused CO2 laser on equine skin: a histological examination

REASONS FOR PERFORMING STUDY: No studies have been published on effects of treatment with a defocused beam carbon dioxide (CO2) laser on equine skin histology. A better understanding of this will help to define how lasers should be used, in order to reduce potential side effects. OBJECTIVE: To describe the acute effects of different doses of defocused CO2 laser, ranging from therapeutic to surgical levels, on equine skin. METHODS: Defocused CO2 laser was administered to the skin in the hamstrings (91 J/cm2), fetlock (137 J/cm2) and loin (450 J/cm2) areas of 13 Standardbred horses. The acute effects on skin histology were examined 90 min after the end of therapy. RESULTS: Mild changes with focal spongiosis and subepidermal clefts were found after 91 J/cm2 irradiation and more severe changes with diffuse subepidermal clefts after the 137 J/cm2 dose. A homogeneous eosinophilic acellular zone of dermis and destruction of adnexal structures, and significant thinning of the epidermis was observed after the 450 J/cm2 dose. CONCLUSIONS: The present study indicates acute dose-dependent changes in equine skin histology after laser treatment Severe tissue damage was induced using a 450 J/cm2 dose. POTENTIAL RELEVANCE: To reduce the potential side effects of defocused CO2 laser treatment, the laser parameters must be carefully evaluated. Caution should be taken if doses higher than 91 J/cm2 (16 W, 4 min, and 42 cm2) are used in irradiation of equine skin.     

重离子12C6+治疗小鼠移植性肿瘤S180的试验研究

从实验肿瘤学、肿瘤病理学等角度对碳离子治疗小鼠移植性肿瘤S180进行了研究，结果表明，在有氧和急性缺氧情况下碳离子可有效治疗小鼠移植性肿瘤，碳粒子照射后主要引起肿瘤组织的坏死变化。     

Fluorescence detection of a new photosensitizer, PAD-S31, in tumour tissues and its use as a photodynamic treatment for skin tumours in dogs and a cat: a preliminary report

We describe here the detection by fluorescence of a new photosensitizer, PAD-S31, in tumours in dogs and cats and the effect of photodynamic therapy (PDT) by using PAD-S31 for skin tumours in two dogs and one cat. PAD-S31 is a hydrophilic photosensitizer that has two peaks at absorption wavelengths 406 and 665 nm in distilled water. In a preliminary experiment in mice transplanted with SCCVII and colon 26, PAD-S31 was retained in tumour tissues rather than in other organs. The tumours resected from dogs and cats after intravenous administration of PAD-S31 at a dose of 15 mg/kg emitted strong red fluorescence under light illumination of 402 nm wavelength. Animals given PAD-S31 showed no cutaneous photosensitivity under room light illumination. Irradiation at laser light 670 nm wavelength (fluence rate 150 mW/cm2 and total light dosage 150 J/cm2) on cutaneous mast cell tumours in dogs ( n=2 ) and a cutaneous basal cell tumour in a cat induced complete remission. These results suggest PAD-S31 could be a promising photosensitizer for use in a small animal veterinary practice.     

淫蜂佐剂对实验动物NK细胞活性的影响

用微量乳酸脱氢酶（LDH）释放法分别观察了淫羊藿－蜂胶佐剂（以下简称“淫蜂佐剂”）对小鼠脾脏和雏鸡外周血中自然杀伤细胞（NK细胞）活性的影响。结果：小鼠NK细胞活性在淫蜂佐剂（Ⅰ）组、生理盐水对照（Ⅳ）组、淫蜂＋环磷酰胺（Ⅱ）组和环磷酰胺（Ⅲ）组的上述各组间差异显著或极显著（P＜0．05或P＜0．01）（Ⅱ组和Ⅳ组间差异不显著，P＞0．05），这种差异在4周龄小鼠较8周龄小鼠更为明显。雏鸡NK细胞活性，35日龄时，注射淫蜂佐剂雏鸡极显著高于注射生理盐水雏鸡，0．4mL／羽组明显高于0．2mL/ 羽组。上述结果表明，淫蜂佐剂能提高实验动物NK细胞活性，并拮抗环磷酰胺对NK细胞活性的抑制作用。淫蜂佐剂的这种作用在适龄的幼年动物中表现更为显著，并存在一定的量效关系。