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鲤鱼HSP70基因组织表达差异研究 总被引:2,自引:0,他引:2
1材料与方法
1.1材料 供试鲤鱼购自成都市洗面桥市场。主要试剂:TR—Iaol总RNA提取试剂盒购自Invitrogen公司,反转录试剂盒、pMD-18T载体、D12000DNA和如DNA聚合酶购自大连宝生物工程有限公司;琼脂糖凝胶DNA回收试剂盒购自Axygen公司;DEPC原液(Sigma分装)、JM-109感受态细胞购自大连宝生物工程有限公司,其他试剂均为国产分析纯。 相似文献
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cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harboring MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines. 相似文献
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通过基因工程手段抑制甘蓝型油菜Δ12-油酸去饱和酶(delta-12 oleate desaturase FAD2)基因的表达,从而使油酸脱饱和产生亚油酸的步骤受阻,达到富集油酸,减少多不饱和脂肪酸含量的最终目的。依据植物RNA干扰的原理和研究中用于构建RNAi载体的基本经验,选择油菜fad2基因片段(510bp)分别以反向和正向的形式插入到油菜napin启动子下游,并在反向和正向插入的基因片段之间即间隔区导入1个来源于豌豆的rbcS-3C基因内含子(83bp)及其剪切位点之前5bp、之后4bp片段。组装完成的fad2RNAi载体转入到植物双元表达载体pCAMBIA3301,植物筛选标记基因采用抗灭生性除草剂PPT的选择基因bar及报告基因gus,从而构建成以甘蓝型油菜fad2基因为靶标的RNAi植物表达载体pCAMBIA3301-fad2i。 相似文献
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PttGA20-氧化酶基因dsRNA抑止载体的构建(英文) 总被引:1,自引:0,他引:1
[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant. 相似文献
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《(《农业科学与技术》)编辑部》2008,(4)
[Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.[Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.[Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.[Conclusion] The experiment provided a new way for culturing dwarfed plant. 相似文献
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为进一步研究瓜叶菊Mlo基因的功能以及为将来获得具有广谱与持久的白粉菌抗性瓜叶菊种质资源奠定基础,以瓜叶菊(Pericallis hybrida B.Nord)‘大花’为试验材料,克隆瓜叶菊‘大花’Mlo基因保守片段,构建了瓜叶菊‘大花’RNAi载体;同时构建了瓜叶菊‘大花’高频再生体系;并利用根癌农杆菌介导法转化瓜叶菊‘大花’进而建立了遗传转化体系。经酶切鉴定,成功构建了Mlo基因的RNAi载体质粒pTCK303-mlo-RNAi,构建了瓜叶菊‘大花’高频再生体系,最终确定瓜叶菊‘大花’最佳愈伤诱导培养基为:MS+6-BA 2.4mg/L+NAA 1.0mg/L+KT 0.3mg/L+2,4-D 1.0mg/L;瓜叶菊‘大花’愈伤组织的分化培养最适培养基为:MS+6-BA 2.0mg/L+NAA0.1mg/L;瓜叶菊‘大花’生根培养的最佳培养基为1/2MS+NAA 0.5mg·L-1,并且初步探索了瓜叶菊‘大花’Mlo基因转化体系。 相似文献
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[目的]通过基因工程手段抑止赤霉素调控基因PttGA20-氧化酶基因的表达,从而抑制植物高生长和节间伸长,达到培育矮化植株的目的。[方法]依据RNAi原理,设计引物扩增正义及反义PttGA20ox片段插入pBI121载体CaMV35S启动子下游区域,并在正义和反义片段间隔区插入茎环结构GUS基因片段,将npⅡt标记基因替换为抗除草剂基因bar,构建dsRNA抑止载体。[结果]所构建的抑止载体经经不同内切酶酶切鉴定后均可释放出与目的条带大小相同片段,表明已成功构建PttGA20-氧化酶基因dsRNA抑止载体。[结论]该研究为培育矮化植株提供了新的途径。 相似文献
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利用i TRAQ技术从陆地棉(Gossypium hirsutum L.)HM-40中筛选并克隆到S-腺苷甲硫氨酸合成酶Gh SAMS基因。Gh SAMS基因的开放阅读框全长为1 182 bp,编码393个氨基酸,预测Gh SAMS蛋白质含有15个磷酸化位点,推测其功能的行使可能与激酶磷酸化相关,进化分析表明S-腺苷甲硫氨酸合成酶与茶树(Camellia sinensis)的SAMS蛋白质相似性最高。q RT-PCR分析表明,在接种黄萎病菌VD07菌系后,Gh SAMS表达量逐渐增加,随着接种时间的延长,其相对表达量随之增加,推测其在陆地棉抗黄萎病防卫反应中可能起重要作用。在嫁接棉株中,利用VIGS技术成功地沉默Gh SAMS基因,然后对沉默棉株接种黄萎病菌VD07,鉴定其病情指数为62.5,抗性级别为感病;而转化空载体和未接种载体处理的嫁接棉株病情指数分别为30.0和31.2,抗性级别为耐病,表明Gh SAMS基因沉默后嫁接棉对黄萎病的抗性丧失。推测Gh SAMS基因在陆地棉抗黄萎病的过程中可能起重要作用。 相似文献
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棉花GhCAD6基因表达载体构建及GUS基因的瞬时表达 总被引:2,自引:0,他引:2
[目的]以GUS基因为报告基因,构建GhCAD6基因的瞬时表达载体.[方法]采用PCR方法和基因枪法.[结果]用PCR法,以pGEM-T-CAD6质粒为模板,获得GhCAD6基因目的片段.然后将其克隆到瞬时表达载体pRTL2-GUS/NIa中,获得由CaMV35S启动子调控目的基因的pGUS-CAD6融合表达载体,使目的基因能够和GUS基因同时表达.采用基因枪法将pGUS-CAD6转化到洋葱表皮细胞中,暗培养24 h,经GUS组织化学染色,检测到多个洋葱细胞呈现蓝色.[结论]构建的瞬时表达载体可在植物细胞中高效表达,为进一步研究棉花GhCAD6基因的功能奠定了实验基础. 相似文献