| 外源蛋白定位马铃薯淀粉粒表达载体的构建 |
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| 引用本文： | 李珺,马力通,姚新灵.外源蛋白定位马铃薯淀粉粒表达载体的构建[J].农业科学与技术,2009,10(4):72-74. |
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| 作者姓名： | 李珺 马力通 姚新灵 |
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| 作者单位： | 李珺 ,马力通(内蒙古科技大学,内蒙古包头,014010);姚新灵(宁夏大学生命科学学院,宁夏银川,750021) |
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| 基金项目： | the National Natural Science Foundation Program (30160010).国家自然科学基金项目 |
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| 摘 要： | 1材料与方法
1．1材料马铃薯品系：郑1011。受体菌：大肠杆菌BL21。载体质粒pCambia1305—1购自TaKaRa公司；反转录试剂、TaqDNA聚合酶、T4DNA连接酶、限制性内切酶购自TaKaRa公司；DNA回收试剂盒Wizard PCR preps DNA Purification System购自Promega公司。
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| 关 键 词： | 马铃薯 表达载体 蛋白定位 TaqDNA聚合酶 淀粉粒 T4DNA连接酶 omega公司 限制性内切酶 |
Construction of Vectors to Express Foreign Protein within Potato Starch Grains |
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| LI Jun,MA Li-tong,YAO Xin-ling.Construction of Vectors to Express Foreign Protein within Potato Starch Grains[J].Agricultural Science ＆ Technology,2009,10(4):72-74. |
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| Authors: | LI Jun MA Li-tong YAO Xin-ling |
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| Institution: | 1. Inner Mongolia University of Science ＆ Technology, Baotou 014010 ; 2. School of Biological Science, Ningxia University, Yinchuan 750021) |
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| Abstract: | Objective] The aim is to study the construction of vectors expressing foreign protein in potato starch grains specifically, and provide some reference for solving industrialized core problem of high cost and low expression level of foreign protein. Method] By using molecular biological techniques of RT-PCR and nested PCR, plant expression vector for the foreign protein locating in the potato starch grains was constructed. Result] Coding sequence （ GC20 ） of potato starch grains that was located and expressed by GBSSI promoter was cloned. Plant expression vector was screened out through connection, transformation and enzyme digestion identification. Conclusion] This result laid a foundation for further screening the foreign protein on the potato starch grains. |
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| Keywords: | Foreign protein Plant bioreactor GBSSI GC20 Starch grains |
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