| PttGA20-氧化酶基因dsRNA抑止载体的构建(英文) |
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| 作者单位： | 北京林业大学林木花卉遗传育种教育部重点实验室，华南农业大学林学院 |
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| 摘 要： | Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.Conclusion] The experiment provided a new way for culturing dwarfed plant.
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RNAi Plasmid Construction of PttGA 20-oxidase Gene |
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| Abstract: | Objective] Genetic Engineering technology was used to regulate the expression of PttGA20-oxidase gene thus restrained plant height growth and internode elongation for cultivating dwarfed plant.Method] Based on the RNAi principle,the gene specific sequences of PttGA20-oxidase in the antisense and sense orientations interrupted by a gene sequence from GUS were cloned into a binary vector pBI121.The selection marker gene npt Ⅱ was replaced with bar gene to RNAi plasmid.Result] After undergone different endonuclease restrictions,the constructed constraint vector released different segments whose sizes were similar to that of target segment,which demonstrated that the RNAi plasmid of PttGA20-oxidase gene was successfully constructed.Conclusion] The experiment provided a new way for culturing dwarfed plant. |
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| Keywords: | Gibberellins PttGA 20-oxidase gene RNAi Vector construction |
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