橙皮甙生理效应研究进展

橙皮甙是柑橘果皮中的主要功能活性成分,具有治疗心脑血管疾病、协同抗肿瘤、抗菌消炎、抗氧化、防止紫外辐射等多种生理功效和药理作用,引起了国内外的广泛关注。本文中综述了橙皮甙近年来在保健功效和应用方面的研究现状,包括橙皮甙的结构与理化性质,橙皮甙的抗菌消炎作用、对心血管系统的作用、对抗氧化的作用等生理效应。并对其在保健食品、医药、化妆品等方面的应用前景作了展望。     

橙皮综合利用研究进展

介绍了橙皮中有价值成分香精油、橙皮色素、果胶、橙皮苷等的提取方法以及在食品、饮料、化妆品等方面的应用.     

超声辅助响应面法优化种植佛手中橙皮苷和柚皮苷提取工艺的研究

为了优化提取万州种植佛手中橙皮苷和柚皮苷的工艺条件,采用响应面法优化超声辅助进行研究。在超声提取时间、乙醇体积、超声提取温度、液固比单因素试验基础上,研究了超声条件下影响万州种植佛手中橙皮苷和柚皮苷含量的几个因素,采用Box-Behnken试验设计和响应面分析法考察前述4个条件对万州种植佛手中橙皮苷和新橙皮苷含量的影响,采用RP-HPLC法测定万州种植佛手中橙皮苷和柚皮苷的含量。结果表明,最佳超声辅助提取工艺为超声提取时间26 min、乙醇体积分数82%、超声提取温度62℃、佛手与乙醇的质量比1:22 (g/mL),万州种植佛手中橙皮苷和柚皮苷含量分别为0.072%±0.315%和0.082%±0.753%,与预测值相符。本研究对万州种植佛手超声辅助提取的工艺可靠性高,有效成分提取率高,适合于工业化生产推广。     

桔皮中提取橙皮苷方法的研究

研究了从桔皮中提取橙皮苷的一种新方法。优化条件是：按干拮皮与９５％乙醇质量比１：２０投料,在５０℃下浸提３次,每次４ｈ,总浸提效率达５．３７％。     

Essential Oils and Antioxidants Derived From Citrus By-Products in Food Protection and Medicine: An Introduction and Review of Recent Literature

After a brief overview of the general production, market value, and uses of citrus by-products, this literature review will focus on the most highly refined “pharmaceutical grade” citrus oils and antioxidants that have been derived from peels or other fruit parts. Citrus oils have been used as a part of the perfume industry and in the practice of alternative medicine for a long time, but now there is a great deal of mainstream food processing and drug industry interest in them owing to the rediscovery or confirmation of some of their beneficial properties, as well their development for entirely new applications. Their roles as antimicrobial, immunomodulatory, and cancer chemopreventive or chemotherapeutic agents appear promising.     

Influence of citrus flavonoids on laying hen performance,inflammatory immune response,egg quality and yolk oxidative stability

1. The objective of this study was to investigate the effects of dietary supplementation with natural flavonoids (naringin and hesperidin) on laying hens’ performance, cellular immunity and egg quality parameters.

2. A total of 72 individually caged laying hens were allocated into 1 of 6 treatment groups: a control (C) group that was fed with a basal diet and groups that were offered the same diet further supplemented with hesperidin at 0.75 g/kg (E1), or 1.5 g/kg (E2), or naringin at 0.75 g/kg (N1), or 1.5 g/kg (N2) or α-tocopheryl acetate at 0.2 mg/kg (VE) for 67 d.

3. Supplementation with naringin or hesperidin did not affect the performance and egg quality (P > 0.05) apart from an improvement in the yolk colour that was more orange in naringin and hesperidin groups in comparison to the controls (P-linear < 0.05). Egg yolk and plasma cholesterol levels were not affected by citrus flavonoids (P-linear > 0.05).

4. Inflammatory immune response, measured by phytohaemagglutinin skin test (PHA), was suppressed in laying hens that were fed with either naringin (P-linear < 0.05) or hesperidin (P < 0.05). Egg yolk oxidative stability was improved from the 4th d after naringin or hesperidin supplementation. This beneficial effect was comparable to that of α-tocopheryl acetate and was observed in eggs that were stored for up to 120 d.

5. In conclusion, naringin and hesperidin may favourably prolong the shelf life of eggs, appear to possess anti-inflammatory properties and could improve the yolk colour without any side effects on the performance or egg quality traits.     

柚皮甙、橙皮甙和儿茶素硫酸酯的合成及其HPLC-MS的鉴定

目的合成柚皮甙、橙皮甙、儿茶素硫酸酯。方法柚皮甙、橙皮甙、儿茶素在冰浴中与浓硫酸反应2 h,用6N NaOH终止反应。衍生物用高效液相色谱(HPLC)-大气压化学电离(APCI)-质谱(MS)联用鉴定。结果和结论有柚皮甙、橙皮甙和儿茶素硫酸酯的生成。     

葛根素和橙皮苷对热应激条件下锦江黄牛养分表观消化率和粪便菌群组成的影响

本试验旨在研究饲粮中添加葛根素和橙皮苷对热应激条件下锦江黄牛养分表观消化率和粪便菌群组成的影响。选取4头健康、体况良好、体重在250 kg左右的锦江黄牛，采用4×4拉丁方试验设计，对照组饲喂基础饲粮，葛根素组、橙皮苷组和复合组分别在基础饲粮中添加0.04%葛根素、0.20%橙皮苷和0.04%葛根素+0.20%橙皮苷。全程共分为4个试验期，每个试验期时长为16 d。结果显示：1）试验期间，环境温湿度指数（THI）高于72，表明试验牛处于热应激状态。与对照组相比，复合组的体温显著下降（P<0.05），葛根素组、橙皮苷组的呼吸频率显著下降（P<0.05）。2）与对照组相比，葛根素组的干物质、粗蛋白质和中性洗涤纤维表观消化率显著升高（P<0.05），橙皮苷组的粗脂肪表观消化率显著升高（P<0.05），复合组的粗脂肪和中性洗涤纤维表观消化率显著升高（P<0.05）。3）与对照组相比，葛根素组的Simpson指数显著下降（P<0.05），橙皮苷组的Shannon、Sobs指数显著升高（P<0.05）,Simpson指数显著下降（P<0.05）；葛根素...     

中药橘络质量标准的建立与提高

目的：对市场上流通的橘络药材的质量进行分析研究,拟为其质量标准的提高奠定基础。方法：主要是采用薄层色谱法对橘络进行定性鉴别,采用高效液相色谱法测定橘络中橙皮苷的含量等。结果：TLC法鉴别色谱特征斑点明显。橙皮苷对照品在0.0025～0.5010 mg.mL-1的浓度范围内呈现良好的线性关系,平均回收率为99.1%,RSD为1.3%(n =6),10批橘络样品中含橙皮苷按干燥品计平均为6.19％。结论：本文建立的TLC和HPLC方法专属性强,重复性好,可用于橘络的质量控制.     

超高效液相色谱法测定桔百颗粒中的橙皮苷

本试验建立了超高效液相色谱法测定桔百颗粒中橙皮苷含量的方法.样品经甲醇提取,定容,采用超高效液相色谱分离,以保留时间定性,以峰面积定量.橙皮苷在浓度2 ～ 100μg/mL范围内线性良好,相关系数r为1.0000.平均加样回收率为98.2％,RSD为1.4％.该方法准确、可靠,为桔百颗粒的质量控制和评价提供了依据.