圈养华南虎血液生化指标正常参考值初步研究

以国内部分圈养的健康华南虎Panthera tigris amoyensis作为研究对象,通过收集华南虎血液生化报告和采集华南虎血液样本检测建立样本数据,血样检测采用自动血液生化分析仪干式试剂片生化技术测定,测定血液生化指标包括血糖(GLU)、总蛋白(TP)、总胆固醇(CHOL)、总胆红素(TBIL)、肌酐(CREA)等。得到华南虎血液生化共38项指标的正常参考值范围(95%概率),其中几项主要指标正常值范围如下:GLU(2.82-11.55)mmol/L、TP(56.58-85.93)g/L、CHOL(3.32-8.66)μmol/L、TBIL(0-5.30)μmol/L、CREA(52-349)μmol/L等。统计结果显示,雌雄华南虎间血液生化指标的部分项目有差异(P <0.05),幼龄组和成年组间各项生化指标差异不显著(P >0.05),与其他虎亚种相关血液生化报道的生化指标部分有差别。华南虎血液生化指标正常参考值范围不同于其他虎亚种,建立华南虎血液生化指标正常值对华南虎的饲养管理、疾病诊治预防和保育繁殖研究工作有重要作用。     

不同条件对色木槭种子萌发特性的影响

采用不同质量浓度的IAA和不同浸种时间处理色木槭种子,进行种子发芽率、发芽指数及活力指数测定试验.结果表明:随IAA质量浓度、浸种时间的增加,种子发芽率、发芽指数及活力指数呈先上升后下降的趋势,最适的IAA质量浓度为150 mg·L-1,最佳的浸种时间为21 h,此时的发芽率、发芽指数及活力指数分别为85％、9.6、14.4.     

Modulation of plant root traits by nitrogen and phosphate: transporters,long-distance signaling proteins and peptides,and potential artificial traps

As sessile organisms, plants rely on their roots for anchorage and uptake of water and nutrients. Plant root is an organ showing extensive morphological and metabolic plasticity in response to diverse environmental stimuli including nitrogen (N) and phosphorus (P) nutrition/stresses. N and P are two essential macronutrients serving as not only cell structural components but also local and systemic signals triggering root acclimatory responses. Here, we mainly focused on the current advances on root responses to N and P nutrition/stresses regarding transporters as well as long-distance mobile proteins and peptides, which largely represent local and systemic regulators, respectively. Moreover, we exemplified some of the potential pitfalls in experimental design, which has been routinely adopted for decades. These commonly accepted methods may help researchers gain fundamental mechanistic insights into plant intrinsic responses, yet the output might lack strong relevance to the real situation in the context of natural and agricultural ecosystems. On this basis, we further discuss the established—and yet to be validated—improvements in experimental design, aiming at interpreting the data obtained under laboratory conditions in a more practical view.     

不同宿主源弓形虫ROP16基因的遗传变异分析

为研究不同分离株弓形虫棒状体蛋白ROP16基因的遗传变异，扩增9种不同来源的弓形虫样品的ROP16基因，并对其序列进行比对及构建进化树的分析。结果表明，弓形虫不同分离株ROP16序列的同源性均在990%以上，但存在限制性位点多态性；用弓形虫ROP16作为PCR-RFLP的标记分子，未能鉴别出传统的弓形虫Ⅰ型、Ⅱ型、Ⅲ型，却鉴定出一株特殊的猪源弓形虫分离株。弓形虫ROP16基因的株间保守性提示其是一个用于预防弓形虫病的潜在疫苗候选分子，值得对其进行深入研究。     

NaCl对不同抗性水稻氮代谢及相关基因表达的影响

为了探究盐胁迫下不同抗性水稻氮代谢的变化,以抗性品种东稻4号、盐敏感品种日本晴为材料,测定NaCl胁迫下水稻根和叶中氮含量、氮代谢相关酶活及相关基因表达的变化。结果表明,0.3%的NaCl胁迫后水稻根和叶中NO~-_3含量、硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、依赖NADH的谷氨酸合酶(NADH-GOGAT)活性均有不同程度的下降,而NH~+_4含量、依赖Fd的谷氨酸合酶(Fd-GOGAT)、谷氨酸脱氢酶(GDH)、天冬氨酸转氨酶(AST)活性均升高,东稻4号氮含量及氮代谢酶活性比日本晴稳定,根和叶中变化一致。检测氮转运及代谢相关基因表达发现,硝酸根转运蛋白基因OsNRT1;1、OsNRT1;5、OsNRT1;8、OsNRT2;1,铵转运蛋白基因OsAMT1;1、OsAMT1;2及谷氨酰胺合成酶基因OsGS1;2、天冬氨酸转氨酶基因OsAS盐胁迫后在2个品种中均升高,在高表达部位升高显著;谷氨酸合酶基因OsFd-GOGAT在两品种叶中升高显著,根中稍有下降;谷氨酸合酶基因OsNADH-GOGAT和谷氨酸脱氢酶基因OsGDH在日本晴根中升高显著,在叶中及东稻4号中升高但不显著。OsNR1在NaCl胁迫后两品种叶中表达量显著降低,根中无差异。东稻4号OsNRTs及氮代谢相关基因表达量总体上高于日本晴,而日本晴OsAMTs升高幅度大于东稻4号。研究表明,NaCl胁迫下,东稻4号相比日本晴更能保持氮代谢稳定性,这可能是水稻抗性品种耐盐的一个重要机制。     

花生AhPEPC1基因抑制表达的转基因后代转录组分析

磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvatecarboxylase,PEPC)是控制油料作物种子中蛋白质/油脂含量比率的一个关键酶。本研究检测了花生AhPEPC1基因抑制表达的转基因株系种子含油量,与非转基因花生相比,转基因花生种子含油量提高了5.7%～10.3%。利用转录组测序(RNA-Seq)技术分析花生中AhPEPC1基因的抑制表达是否影响其他基因的功能。结果表明,转录组分析筛选到110个基因差异表达,其中25个基因上调表达,85个基因表达下调。对110个差异表达基因进行了KEGG富集分析,其中有34个基因成功获得了KEGG注释,发现氨基酸的生物合成途径中有2个基因(Aradu.M0JX8,Aradu.FE0Z7)下调表达。利用荧光定量PCR分析了15个DEG(differential expressed gene)在非转基因对照和转基因花生种子中的表达情况,发现其趋势与转录组测序结果基本一致。研究结果可在一定程度上解析AhPEPC1基因调控花生种子含油量的分子机制。     

华南虎皮肤成纤维细胞的分离培养

应用酶消化法（胶原酶消化：0.1%胶原酶Ⅰ型＋0.1%BSA;胰蛋白酶消化：0.25%胰蛋白酶＋1mM EDTA）和植块法从华南虎皮肤组织中分离成纤维细胞。研究结果表明,植块法较适合分离华南虎皮肤成纤维细胞,胶原酶消化法次之,而胰蛋白酶消化法不宜用于分离华南虎皮肤成纤维细胞。DMEM/F12（1：1）培养基＋10%FCS＋10 ng/ml EGF＋5μg/ml胰岛素＋100 IU/ml青霉素＋100μg/ml链霉素组成的培养体系适用于华南虎皮肤成纤维细胞的体外培养。     

Effect of peroxisome proliferator-activated receptor γ agonist rosiglitazone on intestinal injury in rats undergoing orthotopic autologous liver transplantation by inhibiting inflammatory response

AIM: To investigate the effect of rosiglitazone, a peroxisome proliferators-activated receptor γ(PPARγ) agonist, on the expression of PPARγ, the activation of NF-κB and intestine injury in the rats undergoing orthotopic autologous liver transplantation(OALT).METHODS: Sprague-Dawley male rats were randomly divided into 4 groups:control group, sham group, OALT group and rosiglitazone(0.3 mg/kg, iv) pretreatment(ROS+OALT) group. The OALT model was established, and the intestinal tissues were collected 8 h after the liver reperfusion. The intestinal tissue sections were stained to visualize the damage. The expression of PPARγ and NF-κB in the tissues, the concentrations of diamine oxidase(DAO) and fatty acid-binding protein 2(FABP2) in the serum and the concentration of TNF-α and IL-6 in the tissues were measured.RESULTS: Compared with sham group, the intestinal mucosa of the rats showed obvious pathological injury after liver reperfusion in OALT group and ROS group, the Chiu,s scores of intestinal mucosa was significantly higher, and the serum concentrations of DAO and FABP2 increased(P<0.05). After rosiglitazone pretreatment, the injury of intestinal mucosa of the rats was alleviated, the Chiu,s scores was lower and the serum concentrations of DAO and FABP2 decreased(P<0.05), the PPARγ expression was obviously up-regulated in the intestinal tissues, the nuclear translocation of NF-κB was reduced and the concentrations of IL-6 and TNF-α were decreased.CONCLUSION: During perioperative period of OALT in rats, the inflammatory responses are obvious. Furthermore, obvious intestinal injury occurs. PPARγ agonist rosiglitazone obviously up-regulates PPARγ expression and inhibits the inflammation in the intestines, thus protecting against intestinal injury in rats undergoing OALT.     

流域次降雨侵蚀产沙的BP神经网络模拟

在分析黄土高原韭园沟流域多年观测资料的基础上,应用BP神经网络建模方法,建立了流域次降雨侵蚀产沙的神经网络模型。通过输入模型变量流域次降雨量、平均降雨强度、径流深和洪峰流量模数,对流域次降雨侵蚀产沙量进行了训练和预测。预测结果表明,所建BP神经网络模型预测精度较高,可近似揭示复杂非线性流域次降雨侵蚀产沙系统的产沙规律,为建立较高预报精度的黄土高原流域次降雨侵蚀产沙预报模型提供了依据。     

Weeds of East Pakistan

Abstract

In a weed survey, 88 species of weeds, belonging to 30 families, were found growing in the arable lands of the East Pakistan Agricultural University Farms. In East Pakistan, hand-weeding is still almost the only means used for controlling weeds in crop fields. The results of trials conducted at the East Pakistan Agricultural University for controlling weeds in jute are reported.