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山西省枣树上啤酒花矮化类病毒的检测及序列分析 总被引:1,自引:0,他引:1
[目的] 从枣树样品中分离鉴定啤酒花矮化类病毒(HSVd)。[方法] 从山西省农业科学院果树研究所国家枣种质资源圃采集70份枣树叶片样品,提取小分子RNA后通过Northern杂交、RT PCR进行检测,并对阳性样品中的类病毒进行克隆测序,利用生物学软件对所得序列进行分析。 [结果] 70份枣树样品中有1份样品感染HSVd,克隆测序后,共获得13条HSVd序列,它们与GenBank上首次报道的HSVd序列相似性为92.6%~92.8% 。[结论] 本研究首次在国内报道了枣树上分离得到的HSVd序列,HSVd枣树分离物与已报道的HSVd分离物差异较大。 相似文献
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Sugarcane bacilliform virus(SCBV) was detected by PCR from sugarcane showing chlorosis and mottle symptom from Kaiyuan, Yunnan Province.Part sequence of replicase gene of the isolate SCBV-Kaiyuan was determined.Sequence analysis indicated that the 589 bp of SCBV-Kaiyuan shared identities of 73.2%-74.0% and 83.1%-84.1% at nucleotide and amino acid levels with SCBV-Australia respectively, 66.7%-68.4% and 65.6%-67.7% with SCBV-Morocco.The quality and yield of the sugarcane infected with SCBV-Kaiyuan was also investigated.The juice extraction, sucrose content, gravity purity and average stalk weight were decreased 1.55%, 1.24%, 2.22% and 0.26 kg in plants infected with SCBV-Kaiyuan, but reducing sugar was increased by 0.21% in infected plants. 相似文献
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病毒侵染直接影响草莓的生长发育及果实品质,快速检测及鉴定病毒是病毒防治的前提。本研究以‘丰香’草莓和‘哈尼’草莓为试材,利用小RNA(sRNA)测序结合RT-PCR技术对2种栽培草莓品种中存在的病毒进行了研究。对sRNA测序结果进行组装和注释,结果表明:在混合样品中共有242个contigs比对到5种草莓病毒,分别为草莓白化病毒(strawberry pallidosis associated virus,SPaV)、草莓镶脉病毒(strawberry vein banding virus,SVBV)、草莓斑驳病毒(strawberry mottle virus,SMoV)、草莓毛形病毒3(strawberry crinivirus 3,SCrV 3)和草莓毛形病毒4(strawberry crinivirus 4,SCrV 4)。利用RT-PCR技术对sRNA测序结果进行验证,结果表明:SMoV、SVBV和SCrV 3在‘丰香’草莓和‘哈尼’草莓中均检测到,而SPaV和SCrV 4只在‘哈尼’草莓样品中检测到。本研究利用sRNA测序技术鉴定了2个草莓品种中存在的病毒,首次在我国生产的同一个草莓品种中检测到5种病毒,为草莓病毒的检测和防控提供了新的思路。 相似文献
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中国锦紫苏类病毒的检测及其分子生物学特征研究 总被引:4,自引:0,他引:4
从无明显症状表现的11株锦紫苏叶片中抽提低分子量的RNA,经Return-PAGE、RT-PCR和Dot-blot hybridization检测,结果表明11株锦紫苏全部带有锦紫苏类病毒(Coleus blumei viroid,CBVd)。将部分PCR产物克隆到pGEM-3Zf(+)载体上并进行DNA序列测定。序列分析结果,所克隆的序列(GenBank登录号分别为DQ178395、DQ178396、DQ178397、DQ178398和DQ178399)与GenBank中报道的锦紫苏类病毒1号(CBVd-1)序列同源性为85.23%~99.20%。从市场上购买的锦紫苏种子经Return-PAGE和RT-PCR检测不携带锦紫苏类病毒。这是中国发生的锦紫苏类病毒的首次报道。 相似文献
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地高辛标记cDNA探针检测苹果茎痘病毒 总被引:3,自引:0,他引:3
Partial sequence(314 bp) of ASPV was cloned and used as a probe labelled with digoxigenin-11dUTP. The total RNA extracted from samples with Apple stem pitting virus and a series of dilutions of plasmid with ASPV-cDNA were detected by dot blot hybridization. The results showed that the probe was sensitive and specific. The probe couldn't hybridize with total RNA of Apple stem grooving virus, Apple mosaic virus and Apple chlorotic leaf spot virus samples as well as negative control, only hybridized with that extracted from dormant shoot infected with ASPV. The sensitivity for detection of plasmid contained ASPV-cDNA was 1.64 μg. 相似文献
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