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| 苹果锈果类病毒山东栖霞分离物的分子鉴定及序列分析 | |
| 引用本文： | 马伟,姜冬梅,陈丽芳,张志想,李世访,贺振,史学功,王红清.苹果锈果类病毒山东栖霞分离物的分子鉴定及序列分析[J].植物保护,2011,37(2):91-94. |
| 作者姓名： | 马伟 姜冬梅 陈丽芳 张志想 李世访 贺振 史学功 王红清 |
| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100193;中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100193 2. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100193 3. 山东省栖霞市果业发展局,栖霞,265300 4. 中国农业大学农学与生物技术学院,北京,100193 |
| 基金项目： | 现代农业产业技术体系建设专项资金 |
| 摘 要： | 本研究以采自山东省栖霞市的两个苹果样品为试验材料，利用二维电泳、斑点杂交、RT PCR等分子生物学技术对样品中的苹果锈果类病毒(ASSVd)带毒情况进行了检测。结果表明：两个样品均携带ASSVd。对两个样品中的ASSVd进行克隆测序，并利用生物学软件DNAMAN对所得序列进行分析，结果表明：与世界上首次报道的ASSVd序列(X17696)相比，本研究所得到的ASSVd序列变异较小，相对比较保守。 |
| 关 键 词： | 苹果锈果类病毒 检测 序列分析 |
Molecular identification and sequence analysis of the Apple scar skin viroid isolated from Qixia in Shandong Province |
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| Ma Wei,Jiang Dongmei,Chen Lifang,Zhang Zhixiang,Li Shifang,He Zhen,Shi Xuegong,Wang Hongqing.Molecular identification and sequence analysis of the Apple scar skin viroid isolated from Qixia in Shandong Province[J].Plant Protection,2011,37(2):91-94. | |
| Authors: | Ma Wei Jiang Dongmei Chen Lifang Zhang Zhixiang Li Shifang He Zhen Shi Xuegong Wang Hongqing |
| Institution: | Ma Wei1,2,Jiang Dongmei2,Chen Lifang2,Zhang Zhixiang2,Li Shifang2,He Zhen2,Shi Xuegong3,Wang Hongqing1(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing100193,China,2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,3.Qixia Development Bureau of Fruit Industry,Qixia265300,China) |
| Abstract: | Two samples,collected from Qixia in Shandong Province,were detected for Apple scar skin viroid(ASSVd) by 2-dimension polyacrylamide gel electrophoresis(2D-PAGE),dot-blot hybridization and RT-PCR.The detection results demonstrated that both of the two samples were infected with ASSVd.After the two isolates were cloned and sequenced,we used biological software DNAMAN to analyze the sequences.The results suggested that the isolate was relatively conservative and the variation was little when compared with the ... |
| Keywords: | Apple scar skin viroid (ASSVd) detection sequence analysis |
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