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1.
The effects of schisandrin B (Sch B) and its analogs on solar irradiation-induced oxidative injury were examined in BJ human fibroblasts. Sch B and schisandrin C (Sch C) increased cellular reduced glutathione (GSH) level and protected against solar irradiation-induced oxidative injury. The photoprotection was paralleled by decreases in the elastases-type protease activity and matrix-metalloproteinases-1 expression in solar-irradiated fibroblasts. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production. The results suggest that by virtue of its pro-oxidant action and the subsequent glutathione antioxidant response, Sch B or Sch C may offer the prospect of preventing skin photo-aging. 相似文献
2.
Mei Lin Neo Kah Kheng Lim Sung‐Yin Yang Giun Yee Soong Giovanni D. Masucci Piera Biondi Hin Boo Wee Hiroki Kise James D. Reimer 《水产资源保护:海洋与淡水生态系统》2019,29(6):1002-1011
- Giant clams provide and support valuable functions to coral reefs, as well as represent a sustainable resource for traditional fisheries throughout the Indo‐Pacific region. The Ryukyu Archipelago (southern Japan) is known to be the northern latitudinal limits of giant clam distribution, but there is only limited information in the literature regarding species diversity, status, and distribution in this region.
- In this study, we report findings from a rapid survey in 2016, the first of its kind for the Ryukyu Islands, to determine species distribution and abundance of giant clams (tridacnines) around Okinawa‐jima Island.
- Results indicate the presence of four species with an overall density of 5.03 per 100 m2, from most to least abundant: Tridacna crocea, Tridacna maxima, Tridacna squamosa, and Tridacna noae. The previously reported species Tridacna gigas and Hippopus hippopus were both absent from the surveys. The densities and distributions of tridacnines varied among species and sites, which are likely attributable to efforts in replenishing and protecting stocks of selected species.
- The most abundant species, T. crocea, is an important fishery species in Okinawa, and has been widely cultured and restocked to augment depleted populations. In comparison, restocking efforts for less popular species, such as T. squamosa, have been limited, and their current sizes and numbers suggest recruitment constraints.
- Given the importance of the giant clam fishery in this region and the current declines of various species (except T. crocea), further regulations should focus on the protection of larger and mature clams that function as broodstock necessary to maintain spawning and natural recruitment.
3.
Sui Hyang Kuit Louisa Shobhini Ponnampalam Jol Ern Ng Ving Ching Chong Amy Yee‐Hui Then 《水产资源保护:海洋与淡水生态系统》2019,29(10):1681-1696
- Coastal cetaceans in Southeast Asia are poorly studied and are particularly vulnerable to anthropogenic threats, especially in intensive fishing grounds.
- To investigate the distribution and habitat characteristics of cetaceans in the productive coastal waters of Matang, Perak, Malaysia, boat‐based line transect surveys were conducted between 2013 and 2016.
- The Irrawaddy dolphin (Orcaella brevirostris) was most frequently encountered at 3.87 sightings per 100 km, followed by the Indo‐Pacific finless porpoise (Neophocaena phocaenoides) at 1.72 sightings per 100 km, and the Indo‐Pacific humpback dolphin (Sousa chinensis) at 0.66 sightings per 100 km.
- The mean group size was largest for humpback dolphins (8.4 individuals), followed by Irrawaddy dolphins (6.4 individuals), and finless porpoises (2.8 individuals).
- Humpback dolphins exhibited a clustered distribution concentrated mainly in shallow estuarine waters (<10 m deep and <5 km from river mouths), whereas Irrawaddy dolphins were more widely distributed in farther coastal waters (<15 m deep and <15 km from river mouths), and finless porpoises were mostly found farthest from the shore in coastal waters (10–25 m deep and >15 km from river mouths).
- The spatial distribution of the three cetaceans overlapped minimally, and this is likely to reflect the distribution of preferred prey resources, species interactions, and their differential responses to anthropogenic activities and species dominance.
- The results from our study serve as baseline information for future research, conservation, and habitat management of these vulnerable and endangered coastal cetaceans. Conservation actions are recommended for the Matang area.
4.
Wai Yee Wong Lai Him Chow Tsz Huen Wu Ka Yan Ma Ling Ming Tsang Ka Hou Chu 《水产资源保护:海洋与淡水生态系统》2019,29(12):2204-2218
- Freshwater fauna is highly threatened owing to various human disturbances and a lack of natural connectivity among populations. Immediate conservation actions are vital to save freshwater wildlife from population decline and extinction. Given the paucity of resources, conservation prioritization is essential to guide resource allocation for effective and practical management; however, the incorporation of quantitative ranking approaches in population genetic studies is highly limited.
- This study scrutinized the genetic diversity and examined the population structure of three invertebrates (the atyid shrimp Caridina cantonensis, the pachychilid snail Sulcospira hainanensis, the palaemonid shrimp Macrobrachium hainanense) and five fishes (the Chinese barb Barbodes semifasciolatus, the paradise fish Marcopodus opercularis, the Hong Kong paradise fish Macropodus hongkongensis, the Beijiang thick‐lipped barb Acrossocheilus beijiangensis, and the rosy bitterling Rhodeus ocellatus) in Hong Kong, based on mitochondrial cytochrome c oxidase subunit I gene and control region, respectively. In general, the three fish species of conservation concern (M. hongkongensis, A. beijiangensis, and R. ocellatus) showed low to no genetic diversity while the other species showed high genetic diversity and strong population differentiation.
- This study also investigated the use of Heightened Evolutionary Distinctiveness (HED) estimation on quantitatively prioritizing the 95 populations for conservation, including three previously studied species (the white cheek goby Rhinogobius duospilus, the sucker‐belly loach Pseudogastromyzon myersi, and the striped loach Schistura fasciolata). Only five sites had relatively high normalized HED values (>0.70) for all taxa occurring there, and thus are of relatively higher conservation priority. Many sites harboured evolutionarily distinct populations for only one to three species; therefore, a general plan for all freshwater inhabitants cannot be formulated.
- This comprehensive comparative study highlights the importance of genetic attributes in conservation decision making and sheds light on plausible conservation management in Hong Kong and other highly developed areas with significant freshwater biodiversity.
5.
Su Lai Yee Mon Moe Lwin Aye Aye Maw Lat Lat Htun Saw Bawm Kotaro Kawabe Yasuhiko Wada Shin Okamoto Takeshi Shimogiri 《Animal Science Journal》2021,92(1):e13647
Myanmar indigenous chickens play important roles in food, entertainment, and farm business for the people of Myanmar. In this study, complete mitochondrial D-loop sequences (1232 bp) were analyzed using 176 chickens, including three indigenous breeds, two fighting cock populations, and three indigenous populations to elucidate genetic diversity and accomplish a phylogenetic analysis of Myanmar indigenous chickens. The average haplotype and nucleotide diversities were 0.948 ± 0.009 and 0.00814 ± 0.00024, respectively, exhibiting high genetic diversity of Myanmar indigenous chickens. Sixty-four haplotypes were classified as seven haplogroups, with the majority being haplogroup F. The breeds and populations except Inbinwa had multiple maternal haplogroups, suggesting that they experienced no recent purifying selection and bottleneck events. All breeds and populations examined shared haplogroup F. When 232 sequences belonging to haplogroup F (79 from Myanmar and 153 deposited sequences from other Asian countries/region) were analyzed together, the highest genetic diversity was observed in Myanmar indigenous chickens. Furthermore, Myanmar indigenous chickens and red junglefowls were observed in the center of the star-like median-joining network of 37 F-haplotypes, suggesting that Myanmar is one of the origins of haplogroup F. These findings revealed the unique genetic characteristic of Myanmar indigenous chickens as important genetic resources. 相似文献
6.
Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization 总被引:34,自引:0,他引:34
Defensins (molecular weight 3500 to 4000) act in the mammalian immune response by permeabilizing the plasma membranes of a broad spectrum of target organisms, including bacteria, fungi, and enveloped viruses. The high-resolution crystal structure of defensin HNP-3 (1.9 angstrom resolution, R factor 0.19) reveals a dimeric beta sheet that has an architecture very different from other lytic peptides. The dimeric assembly suggests mechanisms by which defensins might bind to and permeabilize the lipid bilayer. 相似文献
7.
J K Yee 《Science (New York, N.Y.)》1989,246(4930):658-661
An 88-base pair fragment in the core promoter of the human hepatitis B virus (HBV) contains a functional promoter and a strong liver-specific enhancer. This enhancer functions in human hepatoma cells, where it is much more active than the previously described HBV enhancer in stimulating expression of the linked bacterial chloramphenicol acetyltransferase gene expressed from heterologous promoters. Studies of the role of this enhancer-promoter in HBV may help to clarify mechanisms of gene expression in cells infected with HBV and the role of the virus in the pathogenesis of hepatitis and hepatocellular carcinoma. 相似文献
8.
Suppression of the neoplastic phenotype by replacement of the RB gene in human cancer cells 总被引:80,自引:0,他引:80
H J Huang J K Yee J Y Shew P L Chen R Bookstein T Friedmann E Y Lee W H Lee 《Science (New York, N.Y.)》1988,242(4885):1563-1566
Mutational inactivation of the retinoblastoma susceptibility (RB) gene has been proposed as a crucial step in the formation of retinoblastoma and other types of human cancer. This hypothesis was tested by introducing, via retroviral-mediated gene transfer, a cloned RB gene into retinoblastoma or osteosarcoma cells that had inactivated endogenous RB genes. Expression of the exogenous RB gene affected cell morphology, growth rate, soft agar colony formation, and tumorigenicity in nude mice. This demonstration of suppression of the neoplastic phenotype by a single gene provides direct evidence for an essential role of the RB gene in tumorigenesis. 相似文献
9.
Wang Z Carter JA Lagutchev A Koh YK Seong NH Cahill DG Dlott DD 《Science (New York, N.Y.)》2007,317(5839):787-790
At the level of individual molecules, familiar concepts of heat transport no longer apply. When large amounts of heat are transported through a molecule, a crucial process in molecular electronic devices, energy is carried by discrete molecular vibrational excitations. We studied heat transport through self-assembled monolayers of long-chain hydrocarbon molecules anchored to a gold substrate by ultrafast heating of the gold with a femtosecond laser pulse. When the heat reached the methyl groups at the chain ends, a nonlinear coherent vibrational spectroscopy technique detected the resulting thermally induced disorder. The flow of heat into the chains was limited by the interface conductance. The leading edge of the heat burst traveled ballistically along the chains at a velocity of 1 kilometer per second. The molecular conductance per chain was 50 picowatts per kelvin. 相似文献