Infections Investigation of ALV and REV in Guangxi Local Poultry Breeds and Sequence Analysis of gp85 Gene of ALV Isolates

In order to investigate the infection status of avian leukosis virus (ALV) and avian reticuloendotheliosis virus (REV) in the major local breeds of Qinzhou,Guangxi,totally 953 samples of egg white,cloaca swab and serum of Ma duck,Shitou goose,Tiejiao-Ma chicken,turkey and pigeon were collected from the representing flocks and detected by the commercial ELISA kits.ALV was isolated for the ALV p27 positive samples by culturing on DF-1 cells,and gp85 gene was sequenced.The results showed that the detections of ALV were negative in the samples except those of Tiejiao-Ma chicken,while REV antibody was found positive in Ma duck,Tiejiao-Ma chicken and turkey.The nucleotide sequences of gp85 gene of two isolates shared 94.5% identity with each other,and shared 86.9% to 94.9% with reference strains.The amino acid sequences of gp85 gene of two isolates shared 91.5% identity with each other,and shared 84.0% to 91.6% with reference strains.There were many variable sites in the hyper variable region hr1 and hr2,and the vr2 and vr3 variable regions were relatively conservative.Phylogenetic tree analysis showed that the two isolates shared the highest homology with SCAU11-XG strain.     

玉米瘤黑粉菌SG200效应蛋白PEP1表达及生物信息学分析

运用生物信息学手段分析pep1基因结构,并根据GenBank中玉米瘤黑粉菌pep1 DNA序列设计引物,从玉米瘤黑粉菌SG200的基因组中扩增得到了pep1基因全长,构建了pET–28a–pep1重组表达质粒,选用大肠埃希菌BL21(DE3)作为宿主菌,以1 mmol/L IPTG诱导表达。SDS–PAGE检测结果表明,诱导表达产物大小与理论值(20 800)一致,说明pET–28a–pep1能够在大肠埃希菌BL21(DE3)中高效表达。     

蛋鸡J亚群禽白血病病毒gp85基因遗传进化分析

为了解福建省蛋鸡J亚型禽白血病病毒(ALV-J)的遗传进化关系,对来自福建省蛋鸡场发病蛋鸡中分离鉴定的3株ALV-J的gp85基因进行克隆与测序,并与国内外18株ALV-J参考株的基因序列进行分析。结果表明:3株蛋鸡分离株的gp85基因与亚群2的国内蛋鸡分离株(CL09DP02、GL09DP01和HuB09JY03)以及原型株HPRS-103的亲缘关系较近,达97.8%~99.6%,表明福建省蛋鸡ALV-J株很可能与国内部分蛋鸡ALV-J分离株有着共同的来源。     

Pathogenesis of HIV-associated dementia and its potential drug targets

HIV-associated dementia (HAD) is a serious complication of AIDS patients. With the wide application of highly active antiretroviral therapy (HAART), the replication of HIV is under effective control and the incidence of HAD is also declined. However, a relatively mild HIV-associated symptom of dementia, called minor cognitively motor disorder (MCMD), become the problem and can not be neglected in the treatment of AIDS. The neural injury caused by HIV may be mediated mainly by macrophages, microglia and astrocytes, though we can not rule out the direct damage on neurons by HIV proteins. To date, the precise mechanism of neural damage caused by HIV remains unclear. The present review tries to figure out the recent progress of pathogenesis and potential drug targets for HAD.     

噬菌体Bp7尾丝蛋白gp37的原核表达与鉴定

为了研究噬菌体尾丝蛋白gp37在识别宿主菌大肠埃希菌K12过程中的作用,PCR扩增获得gp37基因C端1 161bp序列,以EGFP为荧光标签,构建重组表达质粒pET-28a-EGFP-gp37,在大肠埃希菌BL21(DE3)中IPTG诱导表达重组蛋白EGFP-gp37,亲和层析纯化蛋白。SDS-PAGE及Western blot检测结果表明,重组蛋白EGFP-gp37在大肠埃希菌BL21(DE3)为可溶性表达,表达量占菌体总蛋白量的23%。EGFP-gp37融合蛋白与大肠埃希菌K12相互作用,激光共聚焦显微镜检测结果显示,尾丝蛋白gp37能够吸附到宿主菌K12表面,表明尾丝蛋白gp37参与宿主菌受体的识别和吸附过程。     

酵素剂SS-200在菠菜和黄瓜上的施用效果研究

在保护地栽培,高肥力土壤条件下,研究施用天然复合酵素剂SS-200对菠菜、黄瓜生长发育、产量和品质的影响。结果表明:与常规施用化学肥料相比,施用天然复合酵素剂SS-200,有利于菠菜、黄瓜上的生长发育,其叶片数,茎粗,株高都有显著增加;施用天然复合酵素剂SS-200增加了菠菜产量,增产达到24.5%,也提高黄瓜的果实产量,平均1 hm2可以增产13.9%;同时施用SS-200菠菜叶柄中的硝酸盐含量降低了700 mg/L,达到9.2%,而SS-200对黄瓜的可溶性糖和Vc含量影响不大;由于肥料投入的降低及产量的增加,1 hm2菠菜的利润达到55 284元/hm2,黄瓜的利润也达到了67 082元/hm2。     

小区庭院道路照明设计

本文介绍了小区道路照明设计特点，并就设计中遇到的照明控制问题进行了探讨。     

LncFAM200B对牦牛肌内前体脂肪细胞脂质沉积的影响

【目的】拟通过腺病毒介导的超表达及siRNA干扰试验,分析lncFAM200B对牦牛肌内脂肪细胞脂质沉积的影响,为解析lncFAM200B对牦牛肌内脂肪细胞脂质沉积的调控机制奠定基础。【方法】采集牦牛背最长肌组织,采用酶消化法和差速贴壁法分离牦牛肌内前体脂肪细胞;构建包装lncFAM200B超表达腺病毒,设计合成其siRNA干扰序列;通过实时荧光定量PCR（RT-qPCR）分析lncFAM200B超表达与干扰后脂肪分化标志基因PPARγ、C/EBPα、AP2,lncFAM200B潜在靶基因SIRT1、PTEN的表达水平;采用油红O染色、甘油三酯（TAG）测定、CCK-8检测等方法检测细胞内脂滴沉积情况、甘油三酯含量变化及细胞增殖情况。【结果】从牦牛背最长肌成功分离获得肌内前体脂肪细胞;lncFAM200B超表达后,脂肪分化标志基因C/EBPα、AP2表达量显著升高（P<0.05）,随着诱导分化时间的增加,lncFAM200B潜在靶基因SIRT1表达量呈先降低后升高的趋势,PTEN表达量呈现先增高后降低趋势;细胞脂滴沉积显著增加（P<0.05）,且胞内形成较大脂滴;超表达4 d后,细胞内甘油三酯含量显著增加（P<0.05）。干扰lncFAM200B可显著降低脂肪分化标志基因PPARγ、C/EBPα、AP2的表达水平（P<0.05）,降低细胞脂肪沉积,且诱导分化第6天细胞内甘油三酯含量显著降低（P<0.05）;干扰lncFAM200B后其潜在靶基因SIRT1呈现先升高后降低的表达趋势,PTEN则相反。CCK-8增殖实验表明,lncFAM200B超表达72 h后,细胞增殖效率显著降低（P<0.05）,而干扰lncFAM200B后72 h后细胞增殖效率显著增强（P<0.05）。【结论】lncFAM200B可能通过影响脂肪分化标志基因C/EBPα和AP2,脂肪合成相关基因SIRT1和PTEN的表达从而影响牦牛肌内脂肪沉积,但其具体机制还需要进一步研究。     

基于线性光电耦合器的电流检测电路

利用线性光电耦合器HCNR200和运算放大器TLC2262,设计了智能开关电源的电流检测电路。电路具有良好的线性度和高准确度;电路结构简单,实验效果好。