禽波氏杆菌对雏鸡和鸡胚毒力的测定

本文介绍了禽波氏杆菌对雏鸡和鸡胚毒力的测定。测定结果表明,禽波氏杆菌对2日龄公雏腹腔接种的 LD_(50)为1.352×10~4个菌,对12日龄鸡胚尿囊腔内接种的 MLD 低于196个菌。     

检测H9亚型AIV的压电免疫传感器研究(英文)

[目的]研制检测H9亚型禽流感病毒的压电免疫传感器。[方法]用巯基丙酸在镀银电极石英晶体自组装巯基丙酸单分子膜再通过N-乙基-N'-(3-二甲氨基)丙基碳化二亚胺盐酸(EDC)和N-羟基琥珀酰亚胺(NHS)偶联抗H9亚型禽流感病毒的特异性单抗构建传感器芯片,建立可以检测H9N2亚型禽流感病毒的免疫传感器。[结果]该方法具有较好的特异性,不与H5亚型流感病毒和新城疫病毒(NDV)反应,检测灵敏度达到20～100个EID50。[结论]该结果为检测禽流感病毒免疫传感器的进一步深入研究奠定了基础,这为其他相关病毒的监测提供了一种新途径。     

鸡卡氏杆菌的分子鉴定及其16S rRNA基因序列分析(英文)

Two strains of Gallibacterium, isolated from one laying hen flock in Zhengzhou City of Henan Province, were identified by the morphological observation, genus-specific PCR, and analysis of 16S rRNA gene, which was used to generate the phylogenetic tree, with the 21 members of the 12 genera belonging to Pasteurellaceae to analyze the homology. Two strains were named Yu-ZZ-HL-I-SLG and Yu-ZZ-HL-II-GZ. The comparative result of the 16S rDNA sequence shows that the 2 isolated strains are identical in sequence; the highest identity (99.9%) was observed between the isolated strain and one of the strains of Gallibacterium anatis (AF228002), the homologies between the isolated strain and 3 strains of gallibacterium accessed in NCBI (AF228016, Gallibacterium genomosp.1, AF228017, Gallibacterium genomosp.2, AF228018, Gallibacterium genomosp.1) were above 97.1%, higher than that of the isolated strain and the other strains of the other 11 genera which were between 90.7%-93.2%. It can be seen from the phylogenetic tree that the 2 isolated strains and the other 4 strain of gallibacterium fell into the same branch, furthermore the 2 isolated strains and the strain of Gallibacterium anatis locate in an internal branch, indicating that the 2 isolated strains belong to Gallibacterium anatis.     

3个外种猪Nramp1基因多态性研究

采用PCR-RFLP方法,对3个外种猪群Nramp1基因第6内含子序列多态性进行了检测。结果表明,在3个外种猪群中均仅检测到AB和BB2种基因型,其中AB基因型为优势基因型,BB基因型为数极少,未检测到AA基因型。卡方检验结果表明,3个外种猪群该位点均处于遗传不平衡状态（P〈0.01）。群体中该位点杂合度较高、纯合度较低,具有中度遗传多态性,可在后续的育种方案中实施分化选择和培育,为抗病育种提供遗传素材。     

PCR技术在住肉孢子虫虫种鉴定上的应用

采用 RAPD 方法对水牛梭形住内孢子虫、待定种及黄牛枯氏住内孢子虫缓殖子基因组 DNA 进行了分析,待定种 PCR 产物的电泳带型与枯氏住肉孢子虫的差异较梭形住内孢子虫大,这与二者表型性状一致的研究结果不吻合.对3种包囊种特异性的 PCR 指纹进行了筛选,回收和纯化了3种包囊种特异性的 DNA 片段,待定种0.7kb 片段经α-~(32)PdATP 标记后用作探针,只与同源 DNA 杂交,不与其它包囊、宿主细胞和艾美耳球虫杂交,用 pGEM-T Easy Vector 对该片段克隆成功,并测出其全长序列,为进一步设计特异引物建立标准 PCR 诊断方法和用作核酸探针奠定了基础。     

安秦F_1代、和秦F_1代与秦川牛生长性能比较

[目的]为探讨安秦F1代与和秦F1代与秦川牛的生长发育情况。[方法]选择安秦、和秦、秦川公牛各10头作为2个试验组和1个对照组,测定6、12、18、24月龄的空腹体重、体长、体高、胸围数据,并进行比较分析。[结果]表明,在集约化饲养条件下,6～24月龄的安秦F1代体重及各项体尺指标,均显著高于秦川牛（P〈0.05）,和...     

测定王浆腺活性的研究

本文提出了一种新的测定王浆腺活性的方法——10-HDA法,其理论依据是:王浆腺在离体培养时,必然把合成的王浆分泌在培养液中,因此培养液中10-HDA含量,可以直接反映出培养液中的王浆含量,从而间接地反映出王浆腺的活性。     

高寒草甸退化草地土壤特性分析(英文)

[目的]探讨草地退化对土壤理化性状的影响。[方法]研究退化高寒草甸土壤草土比、土壤质地等物理性状,用土壤养分常规分析法分析不同退化草地土壤速效氮、速效磷、速效钾、有效铜、有效锌、有机质及pH等。[结果]随着退化梯度的加剧和土层的加深,草土比逐渐减小;不同退化梯度的草地草土比在土层0～10cm中最大,该层的草土比在0.00100～0.04000变化,土壤类型为壤土类。不同退化梯度上土壤化学性质均发生变化,其中速效氮、磷、钾含量随土层的加深和退化程度的加剧明显减少。退化草地的微量元素铜、锌含量比较缺乏。[结论]各种养分含量和pH与退化草地演替度的大小无显著相关。     

青海大通牦牛肌间脂肪酸组成分析(英文)

[Objective] The study aimed to provide a theoretical basis for rational utilization of Datong yak resource. [Method] By qualitative and quantitative determination with gas chromatograph, the composition and content of intermuscular fatty acid of Datong yak were compared with those of Datong yellow cattle. [Result] Comparing with Datong yellow cattle, Datong yak had lower saturated fatty acid (SFA) content and higher polyunsaturated fatty acid (PUFA) content at extremely significant level (P<0.01), while the difference of monounsaturated fatty acid (MUFA) contents between them was not significant (P>0.05). The PUFA/SFA and n-6/n-3 PUFA of Datong yak were in accordance with the recommended proportions. [Conclusion] The intermuscular fatty acid composition of Datong yak is superior to that of local yellow cattle, which indicates that Datong yak meat has high nutritive value and wide development prospect.     

三峡水库万州大周库湾网箱养鱼对水环境的影响

2005年6月至2006年4月,对三峡水库万州大周库湾投饵式网箱养鱼基地的水质和底泥进行了调查与分析.结果表明:万州大周投饵式网箱养殖对水温、DO、透明度、pH及电导率的影响都不显著.网箱养殖区水温、DO略低于网箱上游和网箱下游.氨氮、硝酸盐、亚硝酸盐、总氮、总磷及正磷酸盐的含量趋势为网箱区最高,其次网箱下游,网箱上游最低,ANOVA分析结果显示差异不显著(p0.05).无机氮的主要形态以硝态氮为主,其次是氨氮,亚硝态氮最少.以总氮和总磷作为氮、磷阈值来分析,网箱上游水质已达到富营养化水平,按地表水环境质量标准(GB3838-2002)评价,网箱上游为Ⅲ类、劣Ⅲ类水质,网箱区为劣Ⅳ类,网箱下游为Ⅳ类水,网箱养殖加重了该水域水质的富营养化.网箱养殖使底泥中氮、磷、硫化物、有机质大量富集.