全文获取类型
收费全文 | 9861篇 |
免费 | 856篇 |
国内免费 | 399篇 |
专业分类
林业 | 2591篇 |
农学 | 458篇 |
基础科学 | 40篇 |
1014篇 | |
综合类 | 3185篇 |
农作物 | 355篇 |
水产渔业 | 1304篇 |
畜牧兽医 | 895篇 |
园艺 | 362篇 |
植物保护 | 912篇 |
出版年
2024年 | 14篇 |
2023年 | 162篇 |
2022年 | 190篇 |
2021年 | 321篇 |
2020年 | 326篇 |
2019年 | 362篇 |
2018年 | 255篇 |
2017年 | 420篇 |
2016年 | 434篇 |
2015年 | 378篇 |
2014年 | 493篇 |
2013年 | 624篇 |
2012年 | 667篇 |
2011年 | 722篇 |
2010年 | 606篇 |
2009年 | 585篇 |
2008年 | 566篇 |
2007年 | 601篇 |
2006年 | 508篇 |
2005年 | 419篇 |
2004年 | 332篇 |
2003年 | 285篇 |
2002年 | 212篇 |
2001年 | 211篇 |
2000年 | 173篇 |
1999年 | 157篇 |
1998年 | 150篇 |
1997年 | 116篇 |
1996年 | 119篇 |
1995年 | 125篇 |
1994年 | 100篇 |
1993年 | 92篇 |
1992年 | 105篇 |
1991年 | 87篇 |
1990年 | 68篇 |
1989年 | 48篇 |
1988年 | 31篇 |
1987年 | 16篇 |
1986年 | 9篇 |
1985年 | 4篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1976年 | 2篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
利用HPLC-ICP-MS方法测定中华绒螯蟹不同形态砷的残留量 总被引:1,自引:1,他引:0
采用高效液相色谱串联电感耦合等离子体质谱仪(HPLC-ICP-MS)测定中华绒螯蟹可食部位6种砷形态的残留量。经过前处理、提取和脱脂后,通过色谱柱、流动相、pH和流速等参数调节,筛选得到最优化的分离和检测条件。结果表明,6种砷形态的混合标准溶液在质量浓度0~50 μg/L范围时,均呈现良好的线性关系,其R2达到0.999,检测限为0.0060~0.0912 μg/kg,定量限为0.020~0.304 μg/kg。在样品中添加0.5、5、50 μg/L浓度水平,回收率良好,范围是70%~120%,相对标准偏差(RSD)均<5%,方法精密度与重复性均良好。本方法为中华绒螯蟹可食部位砷形态残留量建立了有效检测方法,为水产品砷残留快速检测提供技术支撑。 相似文献
3.
Cutaneous leishmaniasis (CL) is an infectious disease that commonly caused by Leishmania (L.) major and L.tropica. Recently there has been a growing interest in proteomics analysis on Leishmania for drug target discovery. Therefore, we aimed to distinguish proteins which might be characteristic for each of the species from those shared by both to the detection of drug targets, which may become helpful for designing new drugs for CL. To identify differences in protein profiles of L. major and L. tropica, we conducted a Sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) analysis. Totally 67 differentially expressed proteins (DEPs) (fold change> 2 and p < 0.05) were identified between species. Of these, 42 and 25 proteins were up-regulated in L. major and L. tropica, respectively. Several enriched GO terms were identified via biological process of up-regulated proteins. Furthermore, the small molecule metabolic process and translation were detected as significant biological processes for up-regulated proteins in L. major, while translation was identified for L. tropica. Also, KEGG analysis has revealed glycolysis/gluconeogenesis and translation as the top pathways in the proteins up-regulated in L. major and L. tropica, respectively. Finally glycosomal malate dehydrogenase was identified as putative drug target using network and homology analyses. The DEPs between the species are essential in host-pathogen interactions and parasite survival in the macrophage. Furthermore, L. major and L. tropica possibly uses different pathogenicity mechanisms that leads to anthroponotic or zoonotic CL. Our results may help in the drug discovery and chemotherapeutic interventions. 相似文献
4.
5.
渠堤化是干扰城镇河流的常见建设方式,掌握植物特征及对其产生显著影响的用地因素,对于优化渠化的城镇河岸植被带具有重要意义。采用样带全面调查的方法,对北京段已基本全线渠堤化潮白河河岸带植物进行调查,通过多重比较分析河岸带植物丰富度在不同空间位置的差异,并利用NMDS非约束排序拟合解译的周边缓冲区土地利用数据,探讨对渠堤化河岸带植物丰富度和种类分布产生显著影响的因素。结果表明:1)河岸带植物种类174种(乔、灌、草分别为52、29、90种),以草本为主;2)全线渠堤化城镇河流河岸带植物群落种类及丰富度均存在建成区和非建成区的显著差异,总体植物、乔木和灌木植物丰富度在建成区显著大于非建成区,但入侵植物沿渠堤化河岸带不存在空间位置的差异,入侵可能沿渠堤蔓延;3)周边缓冲区道路、其他不透水地表面积是城镇地区全线渠堤化河岸带植物丰富度的显著正相关影响因子,对主要观赏灌木和乔木种类分布存在积极影响,对原生灌木和草本种类分布存在消极影响,这种分布特征受人工干扰影响。 相似文献
6.
丁遒劲 《农业图书情报学刊》2021,33(8):71-78
[目的/意义]分析联合目录在资源发现中的功能作用,优化联合目录体系,提升资源发现服务效益。[方法/过程]通过调研国外典型联合目录建设现状,分析联合目录建设发展特征,结合中国文献资源建设现状提出构建新型联合目录体系,自下而上包括协调运行层、系统工具层、数据汇交层、数据管理层和数据服务层。[结果/结论]面向发现服务的联合目录体系特征突出表现在3个方面,参建主体多样化、建设对象同时涵盖印本资源和电子资源,数据、系统和服务的开放性得到明显强化,与数字出版时代的发展要求相一致,能够在一定程度上打破资源发现服务中的馆藏壁垒,进一步推动文献资源的共享利用。 相似文献
7.
Overdose and death of both target and non-target species are not uncommon when α–chloralose is used for animal control. Alpha–chloralose appears to depress the central nervous system by having an affect on the GABA-A receptor in a manner similar to ethanol. Recently central administration of oxytocin was shown to block ethanol-induced impairment in rodents. Our study investigates whether IM administration of oxytocin speeds avian recovery from α–chloralose and reduces mortality under simulated field conditions. We found that when injected twice with oxytocin (30 uL/kg) chickens entered α–chloralose narcosis more quickly and showed greater behavioral impairment. When fed the LD50 dose of α–chloralose, repeated injection with oxytocin produced no significant difference in mortality rate or time to death in comparison to the control group. The effects of peripheral oxytocin on female chicks were different than results found previously in cockerels given oxytocin centrally. Oxytocin is not a suitable antidote for α–chloralose overdose during animal capture in the field but instead accentuates α–chloralose narcosis, making capture more likely and efficient. 相似文献
8.
城市半自然森林具有独特的群落结构和生物多样性特征,研究城市化影响的半自然森林更新特征,可以为城市生物多样性保育与地带性植被恢复重建提供借鉴。文章以上海辰山残存半自然森林1 hm^2固定监测样地为对象,调查分析半自然森林更新苗的物种组成特征。结果表明:1 hm^2样地更新苗共5 937株,分属22科32属37种,其中上海自然分布的乡土种占57.89%,明显高于城市人工林的乡土树种比例;香樟、构树、天竺桂、朴树和小蜡等5种更新苗占总数的57.2%,聚集性分布格局明显;在11个植被群落类型中,各类型更新苗的丰富度普遍较高,不同树种的更新苗存活和发育潜力存在差异。 相似文献
9.
Pugnose minnow (Opsopoeodus emiliae) is a small, reclusive species that is widespread in North America, but is one of the rarest fishes in Canada, found in less than 12 known localities in southwestern Ontario. In contrast to most pugnose minnow populations across the global range, Canadian populations are primarily found in turbid systems, potentially indicating persistence in suboptimal conditions. We used data from a multi-gear species and habitat survey in the Canard River, Ontario, a system dominated by agricultural inputs and the best-known capture site of the species in Canada, to parameterise multi-gear occupancy models for understanding the relationship between pugnose minnow occupancy and microhabitat features, including the role of turbidity. Almost 300 pugnose minnow were captured, representing the largest single collection of the species in Canadian history. The best occupancy model indicated that the probability of pugnose minnow occupancy was highest in the deepest sites with the lowest water clarity (i.e. high turbidity); however, competing models suggested that occupancy was highest at sites with wild celery (Vallisneria americana) and higher water clarity, signifying that habitats with low turbidity may be utilised if sufficient physical cover exists. Together, our results suggest that Canadian pugnose minnow populations occupy and potentially favour turbid conditions, possibly to avoid visual predators in clearer habitats. It remains uncertain whether this abiotic association represents a long-term, viable, local adaptation or whether persistence of pugnose minnow in the Canard River is at risk unless significant water quality improvements can be made. 相似文献
10.
以鱼腥草为材料,以日光灯处理为对照,分析了不同光质LED光源(白、红、黄、绿、蓝LED灯)对鱼腥草叶片活性氧、类黄酮含量及抗氧化酶的影响。结果表明,蓝光下叶片中TBARS(硫代巴比妥酸)含量最高,较对照高30.18%,与希夫试剂染色结果相一致;过氧化氢产生最多,较对照高190.21%,与DAB染色结果一致;超氧阴离子产生速率在蓝光下最高,为对照组的1.54倍,白光下最低;蓝、绿光下脯氨酸含量是对照的2倍;蓝光和黄光处理下,黄酮含量较对照提高了21.4%。蓝、绿光提高了SOD2同工酶的表达,且在蓝光下最高;蓝、绿、红、黄光处理分别诱导了更多的POD基因位点的表达;不同LED光质均提高了CAT抗氧化酶活性,其中蓝、绿光下CAT同工酶的表达量明显高于对照。综上所述,蓝色光处理可以有效提高幼苗的抗氧化能力,可为鱼腥草品质的控制提供参考。 相似文献