共和县绵羊细颈囊尾蚴病感染情况调查

<正>为了掌握青海省共和县绵羊细颈囊尾蚴病的感染情况,制定切实有效的防制措施,2014年1月份、9月份,对本县农业区、牧业区各选10户共880只绵羊进行了投药前、投药后屠宰过程细颈囊尾蚴感染情况调查。本次调查结果表明,未投药前农业区感染率为20%,感染强度1.25(1~5)个;牧业区感染率为35.5%,感染强度1.49(1~5)个,牧业区自然放牧羊细颈囊尾蚴感染明显高于农业区舍饲放牧羊细颈囊尾蚴感染,投药后农业区感染率为     

利用类病毒脂质体抗原检测H5N1亚型禽流感病毒抗体ELISA方法的建立

利用昆虫杆状病毒表达系统制备了H5N1亚型禽流感病毒(AIV)HA蛋白、类病毒脂质体和病毒样颗粒,分别作为包被抗原,建立了相应的检测H5N1亚型禽流感病毒抗体的ELISA方法。特异性试验、敏感性试验、重复性试验和稳定性试验结果表明,利用3种抗原包被所建立的ELISA方法均具有良好的重复性和稳定性,批间和批内变异系数均小于10%,但单独表达的HA蛋白和类病毒脂质体特异性更好,而且类病毒脂质体有更高的免疫反应性,与灭活全病毒相比安全性更高,故在H5N1亚型AIV抗体水平检测方面更具有应用前景。     

产苦马豆素疯草内生真菌Undifilum oxytropis原生质体的制备和再生

以产苦马豆素内生真菌Undifilum oxytropis NX-FEL001为供试菌株,研究了培养方式、菌龄、酶解时间和酶解温度等,对U.oxytropis原生质体制备和再生的影响。结果表明,U.oxytropis菌丝在质量分数为10g/L纤维素酶+10g/L蜗牛酶+1g/L溶壁酶组成的复合酶溶液,35℃恒温,80r/min振荡孵育3h左右,原生质体释放量最高;原生质体在YCM培养基上,22℃培养9d可以再生形成菌落,培养15d其再生率可达8.5%;原生质体在YCM培养基上再生后其菌落颜色与野生型U.oxytropis不一致,呈现白色;菌丝形态与野生型U.oxytropis相一致,经检测再生后菌丝仍然能够产生苦马豆素。U.oxytropis原生质体的制备,将为进行疯草内生真菌的遗传转化和基因操作研究提供重要工具,为进一步开展疯草内生真菌合成苦马豆素的分子调控机制奠定了基础。     

microRNA let-7调控动物个体发育的研究进展

microRNA(miRNA)是一类广泛存在于多细胞动物中的进化保守的大小为18~25nt的非编码小分子RNA,可以通过与靶基因mRNA的非编码区(3′UTR)结合导致mRNA降解,或阻断mRNA翻译而调节基因表达。let-7是在线虫中发现的具有转录后调节功能的小分子RNA,具有高度的保守性,研究发现,let-7参与动物个体多个器官组织的发育过程。作者综述了近年来let-7参与调控脑、神经系统、心肺系统和肌肉发育等组织器官的研究成果,初步阐述了let-7调控组织器官发育的作用机制,以期为进一步探索let-7在动物体内的功能奠定基础。     

贵州某野生动物园长臂猿流感病毒、支原体及巴氏杆菌混合感染的诊治

为弄清贵州某野生动物园长臂猿死亡的原因,本试验采用流行病学调查、临床症状观察、病理剖检诊断和RT-PCR检测确诊等方法,对该野生动物园发病长臂猿进行了诊断。试验结果显示,流行病学调查、剖检病理初步诊断该野生动物园长臂猿疑似流感病毒、支原体和细菌混合感染,RT-PCR/PCR检测确诊发病长臂猿为流感病毒、支原体混合感染,细菌分离培养、生化特性鉴定和动物致病性试验确诊为多杀性巴氏杆菌感染。结果表明造成该野生动物园长臂猿发病死亡的原因为流感病毒、支原体和多杀性巴氏杆菌混合感染。根据诊断及药敏试验结果,制定出了免疫预防及治疗措施。     

浅谈奶牛附红细胞体病防治

<正>附红细胞体病寄生于人和动物细胞表面、血浆骨髓内引起以贫血、黄疸、发热等为主要症状的人畜共患传染病。1病原学附红细胞体呈圆、环形、月芽、网球拍、杆形、逗号形等形态,附在红细胞上单个或成链,可游离在血浆中。姬姆萨和瑞氏染色成紫红色和蓝紫色。以二分裂方式增殖,无性繁殖。一般消毒药几分钟可杀死,较抵温度下呈较强抵抗力。2流行病学几十种动物感染本病,接触、血源、垂直和昆虫传播,人为因素也可传播,四季均可发生,但以夏季     

奶牛腐蹄病的防治

<正>腐蹄病是奶牛养殖过程中常见的一种蹄部疾病。它是由化脓杆菌、金黄色葡萄球菌、坏死杆菌等病原微生物感染引起的蹄真皮和角质层组织发生的化脓性病理过程的一种疾病。若不及时治疗,轻者会引起奶牛泌乳量下降,病牛消瘦,常并发蹄关节炎,严重的会导致蹄壳腐烂、坏死、脱落,或出现败血症,甚至引起死亡。病牛往往卧地不起,不愿站立,站立时病蹄负重差,常半悬提,行走时跛行,有疼痛感,触之避让。检查蹄底时会发现蹄底枕部有黑斑,或嵌满粪污的     

美洲红鹮种鸟繁殖管理技术初探

<正>美洲红鹮(学名:Eudocimus ruber)具有世界最红的鸟类之称。它体态玲珑,行为乖巧,具有极高的观赏价值。美洲红在野外的寿命大约为15年,人工饲养下可达到20年。2012年被列入《世界自然保护联盟》(IUCN)濒危物种红色名录ver3.1——低危(LC)。美洲红属于极高神经质的鸟类,由于自然环境和人工饲养环境差异极大,因此驯养和繁殖难度较高,成功率极低。本文针对美洲红的种鸟繁殖管理技术进行了初步探讨:1美洲红鹮的生物学特性体长56~61cm,翼展     

Pathogen Analysis of Bovine Respiratory Disease Complex

To investigate the pathogen of bovine respiratory disease complex (BRDC) of a dairy farm in Guangxi, a strain of Mycoplasma and a strain of gram-negative pathogenic bacterium were isolated and identified by the means of field surveys, clinic observation, pathological examination, isolation studies and so on.Treatments were taken according to drug sensitivity test results.The Mycoplasma strain, growing on PPLO medium, formed typical "fried egg" colonies.A 448 bp of oppF fragment was amplified by PCR from the strain and had 98.4% nucleotide identity with Mycoplasma bovis reference isolate PG5 of USA.The biochemical features of the gram-negative bacterial isolate were same with Serratia marcescens.The PCR amplified 16S rDNA of the gram-negative pathogenic bacterium strain was 1 400 bp.It shared 99.0% nucleotide identity with other Serratia marcescens reference strains obtained from GenBank.Animal experiment showed that the gram-negative pathogenic bacterium isolate could cause the mice to die.The drug sensitivity tests showed all isolates were sensitive to spectinomycin, azithromycin, amikacin, gentamicin and neomycin.It was effective to treat with dexamethasone and spectinomycin.Pathogen analysis and drug treatment showed that the BRDC was caused by Mycoplasma bovis and Serratia marcescens.     

Effect of Heat Shock Protein-90 (HSP-90) mRNA Expression in Intestinal Tissuse of Chickens Infected with E.necatrix

The 90 chickens were randomly divided into control group, E.necatrix one time infection group and E.necatrix two times infection group.We used duplex PCR method to test the expression changes of HSP-90 mRNA in intestinal tissue of chickens infected with E.necatrix and researched the effects of it comprehensively and systematically.The results were showed that after 0 to 14 days the expression of HSP-90 mRNA in intestinal tissues of 14-day-old chickens infected with E.necatrix presented a upward trend though it was lower than the control chickens.These chickens were infected with E.necatrix again at 28 days, HSP-90 mRNA expressions of duodenum, jejunum and ileum decreased and then increased.However, it always showed a downward trend in appendix.When 28 days chickens were infected with high-does E.necatrix once, HSP-90 mRNA expressions of duodenum, jejunum and appendix were in a declining curve.While it was increased originally and then decreased in ileum.This study indicated that HSP-90 mRNA expressions in intestinal tissue of chickens infected with E.necatrix were inhibited at first, and the expression quantity was closely related to the degrees of intestinal tissue damage.