Relation of Seed Structures to Soybean Cultivar Difference in Pre-germination Flooding Tolerance

Abstract

Flooding during germination inhibits the sprouting and emergence of soybean [Glycine max (L.) Merr.], reducing subsequent growth and yield. Previous studies revealed that cultivars tolerant to pre-germination flooding have a mechanism of reducing water absorption speed (WAS) during the initial stage of inundation; however, seed structures involved in WAS have not been fully clarified to date. The objectives of this study were to identify possible seed structures responsible for cultivar difference in WAS and pre-germination flooding tolerance. WAS of two tolerant cultivars (Peking and Williams) and two susceptible cultivars (Nakasennari and Enrei), which were identified in our previous study, was compared in relation to seed structures. In Peking, WAS was markedly lower than that of the other cultivars, either in intact seed or seed with the seed coat removed, suggesting that both the seed coat and the embryo have a mechanism of reducing WAS in this cultivar. WAS of the hilum side tended to be higher than that of the back side, and sealing of the micropyle significantly lowered WAS, showing that the micropyle rather than the hilum appeared to be responsible for the higher WAS of the hilum side regardless of cultivar. A comparison of cross section area of the hilum revealed that the tolerant cultivars tended to have a larger area than the susceptible cultivars, suggesting that an inner space of the hilum can act as a reservoir at the initial stage of inundation, thereby reducing WAS in tolerant cultivars.     

Effect of Synthetic Cytokinin Application on Pod Setting of Individual Florets within Raceme in Soybean

Abstract

The application of synthetic cytokinin (6-benzylaminopurine, BA) to racemes of soybean genotype IX93-100 at 7 days after anthesis (DAA) enhanced pod-set percentage of the florets at the 5th position and above (numbered from the base on rachis). The endogenous cytokinin (trans-zeatin riboside) content of individual florets was measured at the 1, 3, 5, 7th position every 3 days after anthesis. Cytokinin was detected only from the florets at 9 DAA, and the content was higher in the more proximal florets while it became negligible in the 7th floret. These results suggest that an increase in the amount of cytokinin in individual florets might enhance the pod setting of the florets positioned at the middle or distal part within the raceme.     

Influence of Seed Treatment with Uniconazole Powder on Soybean Growth,Photosynthesis, Dry Matter Accumulation after Flowering and Yield in Relay Strip Intercropping System

Abstract

The relay strip intercropping system of wheat-corn-soybean is widely used in southwest China. However, it is hard to produce soybean stably with this system, since the growth of soybean plants is slower under shading by corn at the seedling stage, and it is compensated by accelerated growth after the symbiotic stage. Soybean plants show excessive vegetative growth due to more rain during the flowering stage, which results in fallen petals, fallen pods and lower yield. This study investigated whether seed treatment with uniconazole powder (0, 2, 4 and 8 mg kg^–1 seed) suppresses excessive vegetative growth of soybean plants during the flowering stage and delays senescence of photosynthetically active leaves at the pod-setting stage. If such events are correlated with changes in photosynthesis, they may affect dry matter accumulation and seed yield in the relay stripping system. Uniconazole promoted biomass accumulation from 31 (R₃) to 61 (R₅) days after flowering (DAF) and seed yield. Seed treatment with uniconazole raised the net photosynthetic rate, stomatal conductance, transpiration rate, and total chlorophyll and chlorophyll a contents. In contrast, uniconazole reduced leaf area index (LAI) from 1 DAF (R₁) to 46 DAF (R₄) with the increase in uniconazole concentration, whereas, uniconazole significantly increased LAI at 61 DAF, and the greatest promotion occurred at 2 mg kg^–1 treatment. The study clearly showed that uniconazole effectively suppressed excessive vegetative growth of soybean during flowering stage, delayed senescence of photosynthetically active leaves at pod-setting stage and induced higher yield, which were related to the changes in photosynthetic rate, chlorophyll content, dry matter accumulation and LAI in the relay strip intercropping system.     

大豆新品种中黄30种植密度试验

为了探索大豆新品种中黄30在承德地区的最佳种植密度，试验采用回归设计法，设种植密度18.0万、22.5万、27.0万、33.8万、36.0万和45.0万株/hm 2计6个处理，对不同种植密度下的大豆病虫害发生情况和产量进行了分析；并对其种植密度与产量的关系进行了回归分析，建立了回归方程。结果表明：在试验设定的密度范围内，随着种植密度的增大，大豆虫食率、褐斑病率和紫斑病率均呈先降低后升高的变化趋势，其中密度为22.5万株/hm 2时，3种病虫害的发生率均最低，分别为0.4％、0.5％和0.1％；大豆产量呈先增加后降低的变化趋势，产量与种植密度的二次回归方程为 Y ＝-2731.4259＋358.9022 X-5.0335X 2。本研究条件下，密度为33.8万株/hm 2时大豆产量最高，为3801.667 kg/hm 2。     

大豆缺蔸效应及在品种区域试验中应用的研究

利用一个主茎型和一个分枝型品系，研究了大豆在不同长度断条下的缺蔸效应。结果表明，分枝型大豆的缺蔸效应大于主茎型大豆，随着断条长度的增加，断条行和相邻全苗行的缺蔸效应均增大。分析了大豆品种区域试验中产生缺蔸效应值，建立了不同类型品种在不同断条方式下的断条长度与实际补偿长度的回归方程。用此方程即可对品种或其它小区试验中的缺蔸效应进行校正。     

Application of the Cropping System Model (CSM)‐CROPGRO‐Soybean for Determining Optimum Management Strategies for Soybean in Tropical Environments

The determination of optimum crop management practices for increasing soybean production can provide valuable information for strategic planning in the tropics. However, this process is time consuming and expensive. The use of a dynamic crop simulation model can be an alternative option to help estimate yield levels under various growing conditions. The objectives of this study were to evaluate the performance of the Cropping System Model (CSM)‐CROPGRO‐Soybean and to determine optimum management practices for soybean for growing conditions in the Phu Pha Man district, Thailand. Data from two soybean experiments that were conducted in 1991 at Chiang Mai University and in 2003 at Khon Kaen University were used to determine the cultivar coefficients for the cultivars CM 60 and SJ 5. The CSM‐CROPGRO‐Soybean model was evaluated with data from two experiments that were conducted at Chiang Mai University. The observed data sets from farmers’ fields located in the Phu Pha Man district were also used for model evaluation. Simulations for different management scenarios were conducted with soil property information for seven different soil series and historical weather data for the period 1972–2003 to predict the optimum crop management practices for soybean production in the Phu Pha Man district. The results of this study indicated that the cultivar coefficients of the two soybean cultivars resulted in simulated growth and development parameters that were in good agreement with almost all observed parameters. Model evaluation showed a good agreement between simulated and observed data for phenology and growth of soybean, and demonstrated the potential of the CSM‐CROPGRO‐Soybean model to simulate growth and yield for local environments, including farmers’ fields, in Thailand. The CSM‐CROPGRO‐Soybean simulations indicated that the optimum planting dates from June 15 to July 15 produced maximum soybean yield in a rainfed environment. However, the planting date December 15 produced the highest yield under quality irrigation. Soybean yield was slightly improved by applying nitrogen at a rate of 30 kg N ha^?1 at planting. Soybean yield also improved when the plant density was increased from 20 to 40 plants m^?2. The results from this study suggest that the CSM‐CROPGRO‐Soybean model can be a valuable tool in assisting with determining optimum management practices for soybean cropping systems in the Phu Pha Man district and might be applicable to other agricultural production areas in Thailand and southeast Asia.     

大豆脂肪酸含量的QTL分析

利用Charleston(♀)×东农594(♂)的F14和F15代永久自交系群体154个单株后代,在2年3点条件下用气相色谱法测得其籽粒5种脂肪酸的含量,利用Win QTL Cartographer2.5复合区间作图法(CIM)进行QTL分析。结果共检测到47个相关的QTL,分布在13个连锁群上。多年多点同时检测到的QTL共有15个,其中控制软脂酸性状的2个,包括qPal-C2-2和qPal-A1-1;控制硬脂酸性状的4个,包括qSt-B1-1、qSt-B1-2、qSt-D1a-1和qSt-C2-1;控制油酸性状的3个,包括qOle-B2-1、qOle-G-1和qOle-H-1;控制亚油酸性状的有2个,包括qLin-C2-1和qLin-H-1;控制亚麻酸性状的4个,包括qLino-B1-1、qLino-C2-1、qLino-D1b-1和qLino-J-1。这些QTL的一致性较高,为特异脂肪酸含量标记辅助育种奠定了基础。大豆脂肪酸含量的主效QTL数量不多,效应大的不多,可能还受许多未能检测出来的微效基因控制。     

大豆高蛋白组合杂交F4代与亲本蛋白质含量的相关性分析

[目的]探讨大豆高蛋白组合杂交F4代蛋白质含量与其亲本的相互关系。[方法]2005年以蛋白质含量高（45.00%以上）或产量高的8个大豆品种或品系为亲本,配制6个杂交组合进行杂交,2008年获得杂种F4代相关资料,分析杂种F4代蛋白质含量与亲本的相关关系。[结果]双亲蛋白质含量中值,尤其是母本蛋白质含量对F4代蛋白质含量仍有很大影响,呈正相关;高蛋白组合F4代蛋白质含量分离较大,蛋白质含量降低,低于双亲;F4代蛋白质含量与单株产量呈负相关,与百粒重呈正相关;F4代蛋白质含量与亲本的熟期差异呈负相关,相关性不显著。[结论]若要提高大豆F4代蛋白质含量,应选择蛋白质含量高的材料作为母本;在F4代单株个体的选择上,为保证后代产量应选择大粒型为主。     

极细链格孢激活蛋白对大豆光合特性、叶绿素荧光参数及产量的影响

为探讨激活蛋白对大豆生长、产量的影响,以大豆冀豆17为研究对象,利用光合仪与称重法测量了不同浓度极细链格孢激活蛋白(1,2,3,4,5,6μg/m L)处理后大豆的光合特性、叶绿素荧光参数及产量。结果表明,随着施用的极细链格孢激活蛋白浓度的增加,大豆叶绿素含量、净光合速率、气孔导度、蒸腾速率、最大光化学量子效率(Fv/Fm)、有效量子效率(ΦPSⅡ)和光化学猝灭系数(q P)呈先上升后下降的趋势。施用1,2,3,4μg/m L浓度的极细链格孢激活蛋白不仅提高了大豆叶绿素含量,而且增加了大豆叶片的净光合速率、气孔导度、蒸腾速率,同时还能提高叶绿素荧光的最大光化学量子效率(Fv/Fm)、有效量子效率(ΦPSⅡ)和光化学猝灭系数(q P)。其中以3,4μg/m L浓度的处理效果最好。施用更高浓度的激活蛋白(5,6μg/m L),上述光合特性与叶绿素荧光促进效果下降或者消失。施用中浓度激活蛋白(3,4μg/m L)大豆产量增加了11.21%~14.76%。结果表明,喷洒合适浓度的极细链格孢激活蛋白是一种促进大豆增产的有效措施。     

向大豆导入几丁质酶基因的初步研究

以根癌农杆菌的Ｔｉ质粒为介导，将抗真菌病的几丁质酶基因，导入默经江省栽培大豆一东农３７号，吉林２８号等１４个品种。从子叶节和下胚轴诱导出丛生芽，并再生植株，经ＰＣＲ和ＤＮＡ分子杂交检测，经ＰＣＲ和ＤＮＡ分子杂交检测的１１３株大豆幼苗中，有７株呈阳性反应，证明几丁质酶基因已导入并整合到大豆的基因组中。