With increasing pressure for a more ecological approach to marine fisheries and environmental management, there is a growing need to understand and predict changes in marine ecosystems. Biogeochemical and physical oceanographic models are well developed, but extending these further up the food web to include zooplankton and fish is a major challenge. The difficulty arises because organisms at higher trophic levels are longer lived, with important variability in abundance and distribution at basin and decadal scales. Those organisms at higher trophic levels also have complex life histories compared to microbes, further complicating their coupling to lower trophic levels and the physical system. We discuss a strategy that builds on recent advances in modeling and observations and suggest a way forward that includes approaches to coupling across trophic levels and the inclusion of uncertainty. 相似文献
Long-term observations of Trichoniscus pusillus revealed that chemical composition and microbial activity of the leaf litter serving as food influenced the reproductive success of females and the mortality rates of juveniles as well. Of the food sources investigated, only alder litter, exhibiting high microbial activity, allowed the maintenance of a stable population with frequently reproducing females. This study concludes that the food type is a significant factor for population parameters of this parthenogenetic isopod. Our results on T. pusillus, belonging to the taxon Synocheta, are compared with data on terrestrial isopod species of the taxon Crinocheta. 相似文献
Dendroclimatological studies in a dry inner Alpine environment (750 m a.s.l.) revealed different growth response of co-occurring coniferous species to climate, which is assumed to be caused by a temporal shift in wood formation among species. The main focus of this study therefore was to monitor intra-annual dynamics of radial increment growth of mature deciduous and evergreen coniferous species (Pinus sylvestris, Larix decidua and Picea abies) during two consecutive years with contrasting climatic conditions. Radial stem growth was continuously followed by band dendrometers and modelled using Gompertz functions to determine time of maximum growth. Histological analyses of tree ring formation allowed determination of temporal dynamics of cambial activity and xylem cell development. Daily fluctuations in stem radius and radial stem increments were extracted from dendrometer traces, and correlations with environmental variables were performed. While a shift in temporal dynamics of radial growth onset and cessation was detected among co-occurring species, intra-annual radial growth peaked synchronously in late May 2011 and early June 2012. Moist atmospheric conditions, i.e. high relative air humidity, low vapour pressure deficit and low air temperature during the main growing period, favoured radial stem increment of all species. Soil water content and soil temperature were not significantly related to radial growth. Although a temporal shift in onset and cessation of wood formation was detected among species, synchronous culmination of radial growth indicates homogenous exogenous and/or endogenous control. The close coupling of radial growth to atmospheric conditions points to the importance of stem water status for intra-annual growth of drought-prone conifers. 相似文献
Isolated carp hepatocytes cultured in serum-free, chemically defined medium were used to investigate within the same cell
preparation characteristics of the binding of insulin as well as effects of insulin on cellular metabolism. The binding of
human [125I]-insulin to carp hepatocytes was studied in kinetic, saturation and displacement experiments. A dependency of insulin binding
on the collagenase used for cell isolation was demonstrated. Insulin binding decreased during the first 12h of culture but
remained constant during the following 12h. The kinetic experiments revealed that [125I]-insulin binding reached a steady state within 20–30 min of incubation. The mathematical analysis of the saturation experiments
demonstrated the existence of two populations of binding sites, one with high affinity (Kd1 = 5.5 pM) and low capacity (Bmax1 = 0.14 fmol/mg protein or 77 binding sites/cell) and one with low affinity (Kd2 = 2.4 nM) and high capacity (Bmax2 = 17.6 fmol/mg protein or 9623 binding sites/cell). In competition experiments, 312 pM [125I]-insulin was displaced by cold insulin, IGF-I and IGF-II with IC50 values of 2.2, 7.9 and 20.3 nM, respectively. Glucagon was without effect. Binding of insulin to carp hepatocytes resulted
in a significant reduction of glucose release and a significant increase of protein synthesis as of de novo fatty acid synthesis.
dedicated to Prof. Dr. W. Hanke on the occasion of his 65th birthday. 相似文献
Like marine fish freshwater fish are an important source of essential fatty acids for human nutrition. However, the fatty
acid composition of pond fish can vary considerably and strongly depends on that of the ingested food. Investigations on the
fatty acid composition of common carp (Cyprinus carpio) and tench (Tinca tinca) have shown that different methods of rearing and feeding cause substantial variations in the proportions of the n-6 and
n-3 polyunsaturated fatty acids of these fish species. Carp reared on the basis of natural food in ponds exhibit high contents
of n-6 as well as n-3 fatty acids in their muscle triacylglycerols. On the other hand carp fed supplementary wheat in ponds
resulted in somewhat lower levels of these essential fatty acids. High amounts of n-3 fatty acids can be found in carp fed
high-energy diets containing high levels of fish oil. Analogous results were obtained in experiments with tench reared under
different nutritional conditions. While rearing on the basis of only natural food in ponds as well as feeding supplementary
wheat yielded in similar levels of n-3 and n-6 polyunsaturated fatty acids, higher contents of n-3 fatty acids were recorded
in tench fed pellets. High levels of n-3 polyunsaturated fatty acids in foodstuffs have positive effects on human health.
Experiments with different cultured fish species proved that the fatty acid composition of the edible parts can be influenced
by the diet. Therefore, a finishing diet with a suitable fatty acid profile can be used to improve the nutritional quality
of fish products of farmed origin. 相似文献
Careful nitrogen (N) management will be needed to nourish the growing human population while minimizing adverse environmental impacts. Aquaponic systems (AS) have a great potential to become a sustainable technology making further use of N-rich aquaculture wastewater. In the present study, we observed the N retention and losses in a running prototype of decoupled AS with Nile tilapia (Oreochromis niloticus) and tomatoes (Solanum lycopersicum) over 24 days. N losses amounted to 32.5% of feed N input and were observed in the recirculating aquaculture system (RAS) of the AS. Fish retained 21.1% of N input while 25.2% of N input accumulated in the RAS water. About 14.1% of the loss of N was caused probably by anaerobic denitrification processes in the lamellar settler (LS). In addition, 18.4% of N input was discharged during the three cleanings of LS. In the hydroponic unit of the AS that has been due to space limitations much smaller than an optimized AS could be (only about 20% of the optimal size relative to fish biomass), the tomato plants, including fruits, leaves, and stems, recovered 3.1% of N input with water uptake of 1700 L. The fish culture management, system design, and environmental management in the greenhouse affect the N recovery in the decoupled AS.
Data on reproductive traits in a cultured population of brown trout (Salmo trutta L.) in southern Chile were collected over three consecutive reproductive seasons: 1999, 2000 and 2001, corresponding to the first (at 3 years), second and third spawnings in females. Data were collected from individual females (n=238, 273 and 169 respectively). The reproductive season lasted mainly from June to August. The peak (July) tended to increase with each season (55.46%, 62.27% and 80.81% of total spawning fish respectively). Female body weight (470.8±102.5, 735.0±150 and 1263.9±263.4 g), total fecundity (1182±344, 1904±595 and 2744±605) and egg diameter (4.64±0.11, 4.67±0.27 and 5.24±0.12 mm) increased significantly (P<0.01) over successive reproductive seasons, particularly between the second and third spawnings. Relative fecundity, on the other hand, decreased significantly with each season (3577±471, 2591±900 and 2181±360). Following analysis of the variables over the three seasons, the correlation pattern of female body weight with total fecundity (r=0.91, n=458; P<0.001) and relative fecundity (r=?034, n=451; P<0.001) proved similar to that described in other salmonid females. Fertilization rate and survival to the eyed egg stage were also positively correlated (r=0.73, n=453; P<0.001). The systematically high values obtained for these latter variables over the seasons evaluated (consistently above 90%) are clearly greater than those we registered in other species of salmonids bred in Chile under similar conditions and suggest highly efficient biological variables that determine the gamete fertilization of this species. The brown trout is, therefore, an interesting potential aquaculture resource in Chile. 相似文献
Summary Unrelated, unselected 2x haploid-species hybrids that produce 2n eggs, by second division restitution and 2n pollen due to
first division restitution were intercrossed to generate 4x progeny. The 19, 4x families were compared with seven cultivars
for tuber yield, tuber appearance and tuber set. The mean yield of the 4x families was 30% higher than the mean yield of the
cultivars. The best five 4x families outyielded the best five cultivars by 57% and 69% at two locations. Cultivars were superior
for tuber appearance and tuber set. The high yields of the 4x families are due to genetic diversity of the 2x parents and
their ability to transmit that diversity to the 4x progeny utilizing 2n gametes. The potential of the 2x×2x breeding method
is dependent on developing 2x hybrid clones that form 2n eggs and possess desirable tuber types; clones producing 2n pollen
and good tuber quality are available. 相似文献
In this study we demonstrate that the demosponge Suberites domuncula harbors a Mn(II)-oxidizing bacterium, a Bacillus strain, termed BAC-SubDo-03. Our studies showed that Mn(II) stimulates bacterial growth and induces sporulation. Moreover, we show that these bacteria immobilize manganese on their cell surface. Comparison of the 16S rDNA sequence allowed the grouping of BAC-SubDo-03 to the Mn-precipitating bacteria. Analysis of the spore cell wall revealed that it contains an Mn(II)-oxidizing enzyme. Co-incubation studies of BAC-SubDo-03 with 100 μM MnCl(2) and >1 μM of CuCl(2) showed an increase in their Mn(II)-oxidizing capacity. In order to prove that a multicopper oxidase-like enzyme(s) (MCO) exists in the cell wall of the S. domuncula-associated BAC-SubDo-03 Bacillus strain, the gene encoding this enzyme was cloned (mnxG-SubDo-03). Sequence alignment of the deduced MCO protein (MnxG-SubDo-03) revealed that the sponge bacterium clusters together with known Mn(II)-oxidizing bacteria. The expression of the mnxG-SubDo-03 gene is under strong control of extracellular Mn(II). Based on these findings, we assume that BAC-SubDo-03 might serve as a Mn reserve in the sponge providing the animal with the capacity to detoxify Mn in the environment. Applying the in vitro primmorph cell culture system we could demonstrate that sponge cells, that were co-incubated with BAC-SubDo-03 in the presence of Mn(II), show an increased proliferation potential. 相似文献