Differential effects of -mannose and 2-deoxy- -glucose on attempted powdery mildew fungal infection of inappropriate and appropriate ramineae

Barley, oat and wheat were used as both inappropriate hosts (IH) and appropriate hosts (AH) for three formae speciales of the fungus Blumeria graminis, the causal agent of powdery mildew disease. Treatment with either the glucose analog 2-deoxy- -glucose (DDG) or with -mannose dramatically suppressed penetration resistance in IH and to a much lesser extent in AH combinations. Other effects of DDG and -mannose were strikingly dissimilar. DDG greatly reduced localized autofluorescence at fungal attack sites on epidermal cells, and prevented hypersensitive epidermal cell death (HR). -mannose had little effect on autofluorescence or HR. DDG arrested the development of fungal haustoria and apparently prohibited biotrophy leading to secondary hyphae. -mannose allowed haustorial development and functional biotrophy leading to the production of elongating secondary hyphae. This suggests that B. graminis is in some way capable of utilizing -mannose as a carbon substrate. Results with IH combinations paralleled those of known mlo -barley responses to DDG and -mannose. Results are discussed in relation to specific physiological processes known to be influenced by either DDG or by -mannose, or by both compounds.     

Characterization of Degraded Soils in the Humid Ethiopian Highlands

Hardpan is a major cause of land degradation that affects agricultural productivity in developing countries. However, relatively, little is known about the interaction of land degradation and hardpans. The objective of this study was, therefore, to investigate soil degradation and the formation of hardpans in crop/livestock‐mixed rainfed agriculture systems and to assess how changes in soil properties are related to the conversion of land from forest to agriculture. Two watersheds (Anjeni and Debre Mewi) were selected in the humid Ethiopian highlands. For both watersheds, 0–45 cm soil penetration resistance (SPR, n  = 180) and soil physical properties (particle size, soil organic matter, pH, base ions, cation exchange capacity, silica content, bulk density and moisture content) were determined at 15 cm depth increments for three land uses: cultivated, pasture and forest. SPR of agricultural fields was significantly greater than that of forest lands. Dense layers with a critical SPR threshold of ≥2000 kPa were observed in the cultivated and pasture lands starting at a depth of 15–30 cm but did not occur in the undisturbed forest land. Compared with the original forest soils, agricultural fields were lower in organic matter, cation exchange capacity, and exchangeable base cations; more acidic; had a higher bulk density and more fine particles (clay and silt); and contained less soluble silica. Overall, our findings suggest that soil physical and chemical properties in agricultural lands are deteriorated, causing disintegration of soil aggregates, resulting in greater sediment concentration in infiltration water that clogged up macro‐pores, thereby disconnecting deep flow paths found in original forest soils. Copyright © 2016 John Wiley & Sons, Ltd.     

动探与承载力关系的研究

通过对动力触探试验与载荷试验的对比,绘制P-S曲线,保留曲线变化均匀,拐点相对明显的数据保留;剔除不理想的试验数据,进行动力触探试验和载荷试验对比分析,总结动力触探与地层承载力关系的规律。     

境外网络游戏的软权力作用分析

境外网络游戏是软权力在文化领域的重要载体之一,正日益凸显其重要地位,大多是通过网络游戏的内容和背景展现出来,意在增加本国经济效益的同时,以达到能够吸引不同国家和地区的更多玩家,灌输和宣传本国的思想和文化,使之成为配合其各国软权力战略实施的双重作用。面对这种新的文化渗透模式,研究如何应对境外网络游戏的隐性影响,是目前研究的主要课题。     

Characterization and quantitative assessment of interspecific and intraspecific penetration of below-ground vegetation by cogongrass (Imperata cylindrica (L.) Beauv.) rhizomes

Cogongrass ( Imperata cylindrica (L.) Beauv.), an invasive C4 perennial grass, negatively influences native plant communities by forming dense monotypic stands that alter ecosystem properties and lower local species diversity. A hypothesized mechanism by which cogongrass achieves competitive dominance is a novel use of below-ground vegetative disturbance of neighboring vegetation (i.e. puncturing). However, very little empirical evidence is found in the peer-reviewed literature to quantify this phenomenon, much less establish it as a true form of competitive interaction. The present field study was conducted to quantify the occurrence of rhizome-mediated, below-ground vegetative penetration, both interspecifically and intraspecifically, document how this phenomenon is influenced by spatial location relative to the cogongrass stand, and determine the role that above-ground and below-ground biomass might have in the process. Analyses of 100 sod samples indicated that rhizome-mediated, below-ground vegetative penetration is a much larger intraspecific phenomenon than an interspecific one. The data also strongly suggested spatial location as a significant factor, with most penetrations occurring in the interior of a cogongrass stand as opposed to the advancing border. Significant correlations between rhizome-mediated, below-ground vegetative penetration and above-ground cogongrass biomass were found in the overall plot analysis and most strongly in the advancing border of the cogongrass stands.     

Comparison ofPotato Virus Y andPlum Pox Virus transmission by two aphid species in relation to their probing behavior

Two different aphid species,Myzus persicae (Sulzer) andHyalopterus pruni (Geoffroy) (Homoptera: Aphididae), were used to analyze their ability to transmit two different potyviruses,Potato virus Y (PVY) andPlum pox virus (PPV), to pepper (Capsicum annuum) andNicotiana benthamiana plants, respectively. In parallel experiments,M. persicae consistently transmitted both viruses with high efficiency, whereasH. pruni always failed to transmit either virus. This is in contrast to previous reports describingH. pruni as a vector of these potyviruses. Different aphid probing behavior among individual aphids of each species was obtained in electrical penetration graph (EPG) experiments performed on pepper plants. This suggested thatH. pruni did not transmit these potyviruses due to behavioral differences during probing that impeded virus acquisition and/or inoculation. It was found thatM. persicae usually makes its first probe within the first 2 min, whereasH. pruni individuals remained for more than 10 min on the plant before starting to probe. Furthermore,M. persicae individuals displayed their first intracellular puncture during the first minute of probing whereasH. pruni needed ∼ 15 min to penetrate the cell plasmalemma with their stylets. In addition, intracellular stylet punctures occurred very frequently forM. persicae but was a rare event, never exceeding a single one, forH. pruni. The relevance of these findings for the epidemiological spread of potyviruses by different aphid species is discussed. http://www.phytoparasitica.org posting May 14, 2006.     

Effects of Clear‐Cutting Alder Coppice on Surface Soil Properties and Aboveground Herbaceous Plant Biomass

The purpose of this investigation was to evaluate the effects of clear‐cutting and skidding impacts on surface soils in an alder coppice and aboveground herbaceous biomass. For this purpose, experimental sites used in the study were a randomized complete block with four replications. Some soil properties were measured at 60 pits at 0 to 5 cm and 5 to 10 cm deep in control, normal harvest, and main skid trail sites. In the main skid trail site, as compared to the control and normal harvest site, the bulk density increased from 0.90 to 1.52g cm^?3, the soil organic‐matter content decreased from 4.77% to 1.65%, and saturated hydraulic conductivity decreased from 86.34 to 9.6 cm h^?1 at 0 to 5 cm deep. Optimization of harvesting time and rehabilitation of skid roads needed to be done to prevent and minimize negative impacts of the skid roads on soils.     

基于芯样的沥青路面抗剪性能评价指标

以沥青路面的取芯芯样为研究对象,采用动载单轴贯入试验方法,通过有限元理论分析和对比试验确定了试验条件和参数,确定以一定作用次数下的贯入深度作为指标,能够较好地评价沥青路面的抗剪切性能.     

初始含水率对SiO2溶胶在两种木材中渗透性的影响

采用两种粒径（30 nm和150 nm）的SiO2溶胶真空浸渍不同含水率（绝干、气干、调湿）的欧洲赤松和南方松边材,比较其24 h内吸液率变化规律和24 h增重率,并通过质量法分析硅溶胶处理材轴向SiO2的浓度梯度,从而考察处理材的初始含水率对SiO2溶胶在木材中渗透性的影响。研究结果表明：（1）两种木材均在绝干状态下吸液率最低,30 nm硅溶胶在气干状态下吸液率最高,而150 nm硅溶胶在调湿状态下吸液率最高。（2）初始含水率对增重率的影响与吸液率不同,30 nm硅溶胶在调湿状态下浸渍木材的增重率低于绝干状态,欧洲赤松气干状态的增重率最高。（3）初始含水率对试材中整体SiO2量的影响与增重率结果基本一致,欧洲赤松中SiO2轴向分布梯度远高于南方松,其渗透的均匀性较差。