Bactrian camel is an ancient and precious species of livestock; that is, unique resources exist in the desert and have important economic and scientific value. In recent years, the number of Bactrian camels has declined sharply. Due to its long reproductive cycle and seasonal oestrus, the mechanism of oestrus is unknown. To identify candidate biomarkers of reproduction, we performed a comprehensive proteomic analysis of serum from Bactrian camel in oestrus and non-oestrus, using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry. We identified 359 proteins, of which 32 were differentially expressed: 11 were up-regulated and 21 were down-regulated in samples from camels in oestrus. We validated the differential expression of a subset of these proteins using qPCR and Western blot. Gene ontology annotation identified that the differentially expressed proteins function in cellular processes, metabolic processes and immune system processes. Notably, five of the differentially expressed proteins, PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2, are involved in reproductive regulatory processes in other animals. KEGG enrichment analysis demonstrated significant enrichment in several cardiac-related pathways, such as ‘dilated cardiomyopathy’, ‘hypertrophic cardiomyopathy’, ‘cardiac muscle contraction’ and ‘adrenergic signalling in cardiomyopathy’. Our results suggest that candidate biomarker (PCGF5, histone H1.2, RBP4, FOLR1 and ANTXR2) discovery can aid in understanding reproduction in Bactrian camels. We conclude that the profiling of serum proteomes, followed by the measurement of selected proteins using more targeted methods, offers a promising approach for studying mechanisms of oestrus. 相似文献
Floods and eolian activities are the dominant external agents to shape the topographic forms in ephemeral desert streams of drylands. So far, few studies have discussed the modern processes of eolian–fluvial interactions. To bridge this gap, we studied the modern interactions of eolian and fluvial process in a desert ephemeral river, the Maobula Gully in Inner Mongolia, which exhibits typical eolian–fluvial interactions.
Materials and methods
Multisource data such as integrated particle size data, hydrological data from the Tugerige Hydrological Station, high-spatial-resolution satellite images, and an eolian sediment saltation emission model were integrated to analyze the effects of eolian and fluvial delivery to the sediment on the riverbed, the eolian sediment feeding rate to the gully, the transport of sediment in flood events, and the interactions between eolian and fluvial processes.
Results and discussion
The desert reach of the Maobula Gully is a replacement reach between coarse sediment from the upper reaches and eolian sediment from the Kubuqi Desert. The annual eolian sediment feeding into the gully exhibited a significant decreasing trend. The eolian sediment into the gully increases the available sediment and the bed roughness, affecting the transport of sediment during floods. The sediment concentration and yields in flood events are mainly decided by the discharge and water yield, respectively. Through a comparison of the channel forms between 1970 and 2013, a recovery mechanism in the Maobula Gully was identified, which involves the equilibrium state between abrupt flood erosion and continuous dune migration.
Conclusions
This study analyzed the modern processes of eolian and fluvial processes and their interactions in a typical ephemeral desert stream named the Maobula Gully, and some interesting results were found. We believe that the methodology and results could provide references and evidence for understanding the mechanisms of fluvial and eolian interactions in other ephemeral desert streams around the world.
Density-dependent non-monotonic species interactions are important in maintaining ecosystem stability and function, but empirical evidences are still rare. Rodents, as both seed dispersers and seed predators, have dual effects on plant regeneration and may result in non-monotonic rodent-plant interactions. According to the non-monotonic models, the relative positive or negative effects of rodents on seedling establishment can be measured based on the positive or negative association of seedling recruitment rate and rodent abundance. In this study, we investigated the fates of acorns of Quercus serrata by tracking tagged seeds on 21 fragmented subtropical islands in the Thousand Island Lake, China. We found that the proportion of germinated seeds of all released seeds showed a dome-shaped association with rodent abundance per seed. The proportion of removed seeds and cached seeds showed a saturated- and a weak dome-shaped association with rodent abundance per seed, respectively. Our results demonstrated a clear empirical evidence that rodent abundance per seed triggered a switch between the relative mutualism and predation in a rodent–seed system. Our study implied that the observed non-monotonic interactions between plants and animals may play a significant role in maintaining biodiversity and ecosystem function. We appeal for more investigations of the complex non-monotonic interactions in various ecosystems. 相似文献