This paper explores the salt-tolerance response of Melilotus species, a forage legume, based on tolerance/susceptibility indices, leaf water relations, leaf/root ion concentrations and multivariate (factor and function discriminate) analyses. In a greenhouse experiment, salinity-susceptibility of 12 accessions of Melilotus (M. albus, M. indicus, M. officinalis) in addition, two controls (alfalfa and Persian clover) were evaluated at 0 mM, 100 mM, 200 mM, and 300 mM of sodium chloride (NaCl). The experiment was conducted in a split plot design with two replications. Stress tolerance score (STS) was calculated based on tolerance/susceptibility indices. Salt-tolerant accessions were identified. An extracted bi-plot based on factor analysis confirmed the results of tolerance/susceptibility indices. There were significant correlations between STS and multivariate statistics. Root potassium cation (K+) concentration was higher in Melilotus species than the control species in all the salinity treatments. STS, root K+ concentration, leaf turgidity, and leaf succulence characteristics can be used for screening salinity-tolerance in Melilotus accessions. In all treatments, M. officinalis, M. indicus, and M. albus were salinity-tolerant species in descending order.
Abbreviations: Total biomass yield under control (Yp) and stress (Ys) mediums, geometric mean productivity (GMP), leaf area (LA), leaf tissue density (LTD), linear regression coefficient (b), mean productivity (MP), relative water content (RWC%), Succulence (S), specific leaf area (SLA), stress susceptibility index (SSI), stress tolerance index (STI), tolerance (TOL), water content at saturation (WCS), water saturation deficit (WSD), sodium chloride (NaCl), potassium cation (K+), sodium cation (Na+), analysis of variance (ANOVA), least significant difference (LSD), alfalfa control accession (CHA), Persian clover control accession (CHC), coefficient of variation (CV), statistics of factor (F) and discriminate function (D) 相似文献
Permeability and water-bearing space are important hydrological characteristics of the loess strata. In this study a systematic experiment was conducted to measure the magnetic susceptibility, grain size, porosity, and infiltration rate of the loess and palaeosol layers on a loess tableland of the central Chinese Loess Plateau, in order to investigate the differences in hydrological conditions between the loess and palaeosol layers. The magnetic susceptibility of the loess layer was lower than that of the palaeosol layer, but the average quasi-steady infiltration rate was about 0.31 mm min~(-1) higher, the coarse silt and very fine sand contents were about7.1% greater, and the porosity was about 5.7% higher. These differences were mainly due to pedogenesis, which was affected by the Quaternary climate. The pedogenesis differences between the loess and palaeosol layers resulted in hydrological property differences in terms of permeability and water-bearing space. The loess layer had a higher permeability and more water-bearing space than the palaeosol layer, which meant that the loess layer is more likely to form aquifers and the palaeosol layer is more prone to form aquitards.The groundwater in the loess strata had a multilayered characteristic, which depended on the relative impermeability of palaeosol layer and the alternate deposition of loess-palaeosol layers. The hydrological characteristics of the loess strata demonstrated that the Quaternary climate had an important control function on the formation and movement of groundwater. This knowledge provides a reliable theoretical basis for water resource development and utilization on the Chinese Loess Plateau, and this study extends the application of Quaternary climate change theory to hydrological systems in loess deposits. 相似文献
为探明发病怀头鲶Silurus soldatovi的病原及其生物学特征,从发病怀头鲶体内分离出1株革兰氏阴性细菌SG-1,通过细菌形态学观察、理化特性、16S r DNA测序和构建系统发育进化树分析对其进行了鉴定。结果表明:分离菌SG-1菌株葡萄糖产气、氧化酶、硝酸盐还原为阳性,鸟氨酸脱羧酶、硫化氢为阴性;进一步采用PCR方法扩增其16S r DNA序列,测序获得片段大小为1409 bp,与维氏气单胞菌Aeromonas veronii模式菌株ATCC35624序列相似性达99.86%;构建系统发育树分析显示,分离菌与维氏气单胞菌自然聚为一支,最终判定SG-1菌株为维氏气单胞菌;人工感染斑马鱼Danio rerio、鲶S.soldatovi,其死亡率均达100%,病鱼呈现体表出血、肛门红肿、腹水等症状;药敏试验结果显示,该菌对头孢克肟、头孢哌酮、头孢噻肟、诺氟沙星、左氟沙星、恩诺沙星、氟苯尼考等药物敏感,对阿莫西林、氨苄西林、磺胺异恶唑、甲氧嘧啶、复方新诺明等耐药。本研究结果为了解维氏气单胞菌感染鲶的致病机理及其该病的预防治疗提供了科学参考。 相似文献